FastQCFastQC Report
Sat 31 Dec 2022
SRR5844725.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844725.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205135
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC10170.49577107758305505No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT8320.4055865649450362No Hit
CCAAGCGGACTGTGAACATTTGTTTTCTTTTTCGTGTGTGTCAAACTAACTG3130.15258244570648596No Hit
CACACTCGGGAAGCTGATGCTGACAACATTTTTGGCAGATGGACTGTGATTT3010.14673263948131718No Hit
ATACAAGGGAATCTTGAATAAAATATTATCCCACTTCTACAGGGGATCCAGG2780.135520510883077No Hit
TAGGCGGGATGGTAAAGATTTAAGAAAGATCCCCTCCTCCTTTGGAAAGGGA2750.1340580593267848No Hit
CACATGGGGCAGCAAGGTCTCTGGATAAAAGATTATCACCCAAATTCAGTTA2740.1335705754746874No Hit
TAGTCAGGGATTGATTTTCACTGCAGTTGAATGAACAAATTATTTCAGTGTG2590.1262583176932264No Hit
CTACCAAGGGGTCTGATAAAAATCAATTCTAGCACTGTGAAGCTTCAGACAC2470.12040851146805762No Hit
ATAACGGGGATCGCTGTGCTCCCGGTTACCCTGGATACCCCGATGAACAGGA2400.11699612450337582No Hit
CTATGAGGGGACCTGTAATTAATCATTTGCTTCTCTTCTAATTTTGTTGTAA2390.11650864065127842No Hit
TCACATGGGAGTTTGATGTATTTAGGGCAATTCTGAAATTCTGAAACACATA2360.11504618909498622No Hit
TACCTTGGGATATGTGTGAATAGTAGCTTCTGTCAATAAGCCCTCTGTAAAT2330.11358373753869402No Hit
TTAACAGCGGGGCAGTAGGCATAATTCAGCAACAAACAATCTTCCTTTGGGA2330.11358373753869402No Hit
ATTCATGGGTAGCATAAACAAATACCAGTTTTGAGATTTGTGAATACACATT2300.11212128598240183No Hit
ACTAACGGGTGTCCTTCCAAGTAGAAACCTTGCAAAGTTGTAGCTTTTTAAA2300.11212128598240183No Hit
CGCACAGGTCTGTAGGAACTTTTAGTTCCATTATATGAACACTGGATAACCT2290.11163380213030444No Hit
AATAACGGGTGTTTAGAACATTCAAGCTATTTGAAGCAATTTCCAAAATGTC2280.11114631827820703No Hit
AAAAATGGGAAGTGTTCATATTCACATAAGAGAATTTTAAATGAAGAAAATT2260.11017135057401223No Hit
TATGCGGGATTTTAATTAATAATGACCACAAAGTATAGGAGTAGTGTTGTTG2240.10919638286981743No Hit
TAGCCTTGGGGTCTGGCGGAGAACTAGCCATCAGCCTCCTGAAGCCTGCCAT2240.10919638286981743No Hit
TGAGTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2230.10870889901772003No Hit
TATCACGGGGAAGCAACTTTTTCTAGTGAGAGCTTTCCTGCTGCAGAAAACC2210.10773393131352524No Hit
TGGTTTGGGAATTAAGGTGAGTAATTAGAGAAATATTGGTTTTATCGAATGA2200.10724644746142784No Hit
TGTTTTGGGTGCCAAGGTTTTAAGATAATTTATTGAAATCTCCTGTAAACTT2200.10724644746142784No Hit
TTAATGGGGATACAGACAAATGATTGCATGAGGCCATGTTTATATGTGTGAC2180.10627147975723304No Hit
ATCCAGGGGCATTCAGACATAGCAATCCAAACCCTTGTTCCTGCTGTAAATG2180.10627147975723304No Hit
TTATGTGGGCAATTTAAAATGTTCTCAAATCAGAAGAATTGCAAAAGAATGC2170.10578399590513564No Hit
CTTGGAGGGAAAATGGAGAAAATATAAAAAGATGTGAAAAAGGAAAAAGGAA2170.10578399590513564No Hit
GCTATGCGGGGTATCCTAGACATGATTTTTTCATTTGCTGCATATTAAACAT2160.10529651205303825No Hit
GACACAGGGAATAAAGACTACTGCAGGAATTAATAATTGAAACACTTATTAA2160.10529651205303825No Hit
TCGTTAGGGGATCTTTAAAGGTAGAATTTTAAATAGGCATGAAATATAGTGA2150.10480902820094085No Hit
GACATTGGGGAGCCTTGGATAAAGGGTCAGGCTCCTTTTTAGTTCAGAGATT2140.10432154434884344No Hit
TACAGTGGGAGAACTTGTTCATTTTCCTCTTAAGTAGCTTCTGATGGGTGTC2140.10432154434884344No Hit
TCACCAGGGGAAAAACAGACACCTGAAAGTCTTCCTCAAAATAACAATCCAG2130.10383406049674605No Hit
GTACTTGGGAAGCTTGTCTTAGAGCAATATGTGGTTTGGCATTTTCTGAGTT2130.10383406049674605No Hit
ATTGTTGGGATATGAACTGTGTTTCCTACTTGATTAAAAAATATAAAGAACT2120.10334657664464865No Hit
ACACTCGGGTCGGCCTCCCAATTAGCTGGAACTGCAGGCACACCACCTCATC2100.10237160894045386No Hit
TTTTTGGGGAGAACATAATTTGTGCTCTCAGAAGCTTACTAGTTAGCAGCTA2100.10237160894045386No Hit
CTCTGCATGGGTGGTTAAATAAACTACTGCACATCCTTACCAAGGGATGGTG2090.10188412508835645No Hit
TATGTTGGGGCTTGTGATTCCTGGGTGGAAACCATGGCAGTGGACAGAAGCA2090.10188412508835645No Hit
AGTTGCGGGGATTCTGAAGCTATTAGAATTTTTTCCCCTGTCACAATAGCAG2090.10188412508835645No Hit
TAAAATGGGGCCCAACCAGCCTTAATTCTTAATAAAGGAAGTTGAAAAATAA2080.10139664123625905No Hit
ATCTCAGGGATATTGAGAAAATCATGACTCTAAATTCTTTAGGATTTATTTT2080.10139664123625905No Hit
GTTAAGGGGAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCCT2080.10139664123625905No Hit
AACAGCGGGGCTTTCTGTTACTGTGTTAACATTTGAGAAATGAGTATTTCAG2080.10139664123625905No Hit
TGTGGCGGAGAATTAGCATGGATATACCGGGACCTCATGCAGCTTGGCAGAT2070.10090915738416165No Hit
TGCATAGGGAGGCATGAGCCACAATACCTGGCCAAGTCCTTTTTTTTAATCA2070.10090915738416165No Hit
GCTTTGGGGTATTTACTAAAATGTAGCATATGCCAGTTATTTTAGAAAATTT2070.10090915738416165No Hit
AAGCTAGGGGGAATACTTAGGATTTGAAAGACAATTAATCAAATTGTTTTTG2060.10042167353206426No Hit
AAAACAGGGCTTTAGGGAATGGAAGTAAGTTCATTTTTAAGTTGTGTTGTCA2060.10042167353206426No Hit
TCCTACGGGAAGAACCTAGGATTCAATTCCAACTTTCTCTGTTTGCCTTGGG2060.10042167353206426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)