FastQCFastQC Report
Sat 31 Dec 2022
SRR5844701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239472
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGCCGTCT14740.6155208124540656No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCAGGACTCATCTCGTATGC13060.5453664729070622No Hit
ACACTCGGGCTAAGGAAACCACTAACAAAGCATAACTTTCAAGTTTAGTAGT3040.12694594775172044No Hit
AAAAGTGGGGATTCAGGTGTGCTGTATTCAGGGTTCCTATCTCATGTGCAAA2870.1198469967261308No Hit
AAACAAAAACGGACAAATGGAATCACTTCAAGTTAAAATGCTTTTGCGCAGC2700.1127480457005412No Hit
TTGCATGGGATTTCTGTTTTGCTTCTGTTTTATGCAAATATAAATCATTTTT2620.1094073628649696No Hit
TGGTCTGGGTGAAGTCTGCCACTAGGAAAACAAAAGCACTAGCTTTTGTTCT2600.10857219215607669No Hit
TGTCAGGGTATTCAGCATCCTGGAACTTATGTGGGATTCAAACTTTATGAAT2600.10857219215607669No Hit
GCACTGGGGACATTGACCTTAGCCAGTTTCTCTTGTCATATTTTCAACTGCC2440.10189082648493351No Hit
TGACGCGGGGGAAGCGAGTAAATAAAAAGAAACGAAGTTGAAAGACTTGCTG2400.10022048506714773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)