FastQCFastQC Report
Sat 31 Dec 2022
SRR5844698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395520
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC15850.4007382686084142No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT15700.3969457928802589RNA PCR Primer, Index 19 (95% over 22bp)
AGCCTGGGGGACAGAGCGAGACTGTCTCAAAAACAAACAAACAAACAAACAA5480.1385517799352751No Hit
GAATTTGGGCTACTTAAGGGATATCTAAAACAATTTCTATACTCTAAAAGAA4410.11149878640776699No Hit
CATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAAG4170.10543082524271845No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)