FastQCFastQC Report
Sat 31 Dec 2022
SRR5844694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275928
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC11550.4185874575976341No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT10070.36495027688382475RNA PCR Primer, Index 19 (95% over 22bp)
GCAAGACTTAAAAAATTCCACTTCTAATTCCCCACCTGCATGGAGGGCACAA3500.12684468412049518No Hit
TTGTTAGGGGCTCCATATCAGATGCTTGATATGGTGCATTGCGGAGGACATA3480.12611985735409237No Hit
CACCTTGGGCATCATTCAGGGATCGTCAGCGTTCACCTATTGCACTTCCTGT3410.12358296367168246No Hit
CGTTAAGGGTACTAGAATTAATGAACTAAAAATTTAAAAGTTACTTTAGCAA3360.1217708967556754No Hit
GATGGATTAATGCCCTCTGAAAGATGTTAAAAATGTGAAACATCACTCCTGC3360.1217708967556754No Hit
AATAGAGGGTACATTTAAACATTAATATTTAAAAGCAGTATTTCATTCTTAA3260.11814676292366125No Hit
CTTTCTGGGCGATCTTAAGCTGGGGCATTCCTAAAATTATTTTCTACTGACC3260.11814676292366125No Hit
AAACGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTC3250.11778434954045983No Hit
ATGTTTGGGGACCTAGATCAAATAGATATCTACAGAACTCTCCACCCAAAAA3170.11488504247484851No Hit
TAACTGGGGATGGGCACTATCACTACAGCTATGGACTCAAGCAACTTCTTAA3140.11379780232524427No Hit
TGCAGAGGGGAATATTTTTAAAATGCTGACCAAATGCCTTTATGTTTTTATA3060.11089849525963294No Hit
TGTACCGGGTACCAAGTATTAATAAGTCAAAAATTCTTTTGTGAAATGTATC3010.10908642834362588No Hit
CATGCCGGGATAATTTAAATTGTAAGACATCATTCACATTTATCAATGTTAC3000.10872401496042446No Hit
TTCAACGGGGACCCGCGGGTTTGCTATGGCGATGAGCAGCGGCGGCAGTGGT2990.10836160157722305No Hit
GCTCAAGGGCTTCTCAGGGCTATTTCAAACTCTACAATCAGATACATTAATA2980.10799918819402164No Hit
TTATTTGGGAACTTGAAGATTGGTTCTCTAAAATAAGATGGACAAAAATAAA2960.1072743614276188No Hit
GGTTATGGGCTGTTAAGAAAAGGGAGTGTTTATGTGTTTGTTTCCAAATATT2960.1072743614276188No Hit
GATACTGGGACTAAAAGACCTTCTGGATGAAGTAATTTTACTAATTTATTCC2910.10546229451161171No Hit
GCTTCGGGGGAGGTAATTTGACACTGCTGCTGGCAGTAGTTCTCTATTCACC2900.1050998811284103No Hit
GTGTTGGGGCATAGCTCTAACATCGATTTGACTTTTAAAATTGTGCTTACTT2880.10437505436200747No Hit
ATTGTCGGGGGATCAGTTTGGCTGAATAGATAGATATACTCATGTATGTAGT2880.10437505436200747No Hit
GCGCTGCGGGAACATAGAAAAAACATTTGACAAAATCTAACACCCATTCATA2860.10365022759560466No Hit
AGCGATGGGATAGCCAAGATTTCATAGTCCTTTAAATGAAACTAGTGTACTC2840.10292540082920182No Hit
TTAGTAGGGTATCAAATGTGTTAAAAGTGCTTAACCCACTGCCAAATATTCT2840.10292540082920182No Hit
ACAACGGGTCTAATGGAACTTTAAAAAATCCATCTCAAAATATTACAGGGAT2840.10292540082920182No Hit
TCGGATGGGATTTCATGGTGTTTCTTAACCTTGTCACCTCCTTTCCTGTACA2830.10256298744600041No Hit
GTAGTTGGGTACACACAGTCAAAAGTTTTCCGTGACTGTGAAATCCAGTGCA2810.10183816067959757No Hit
GTGTTCGGGTCGGGCTGCAAGGACGTCACGGGTCCAGATGAGGAGAGTTTTC2790.10111333391319476No Hit
GATGGAGGGGCTCCTCAGAAGTCGCTTAGCTCTTCGGTGGTTGTCACACGTC2760.1000260937635905No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)