FastQCFastQC Report
Sat 31 Dec 2022
SRR5844693.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844693.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275481
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC11470.4163626529597323No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9300.3375913402376207RNA PCR Primer, Index 19 (95% over 22bp)
TGTACCGGGTACCAAGTATTAATAAGTCAAAAATTCTTTTGTGAAATGTATC3470.12596150006715526No Hit
TTATTTGGGAACTTGAAGATTGGTTCTCTAAAATAAGATGGACAAAAATAAA3200.1161604611570308No Hit
GATGGATTAATGCCCTCTGAAAGATGTTAAAAATGTGAAACATCACTCCTGC3170.11507145683368362No Hit
AGTAACGGGGAAAAAAAGGAAGGAAGGAGAAGGAAGGAAGGAGAAGAAAAGG3170.11507145683368362No Hit
ACAACGGGTCTAATGGAACTTTAAAAAATCCATCTCAAAATATTACAGGGAT3140.11398245251033647No Hit
TTCAACGGGGACCCGCGGGTTTGCTATGGCGATGAGCAGCGGCGGCAGTGGT3130.11361945106922074No Hit
ATGTTTGGGGACCTAGATCAAATAGATATCTACAGAACTCTCCACCCAAAAA3110.1128934481869893No Hit
GGTTATGGGCTGTTAAGAAAAGGGAGTGTTTATGTGTTTGTTTCCAAATATT3100.11253044674587358No Hit
CGTTAAGGGTACTAGAATTAATGAACTAAAAATTTAAAAGTTACTTTAGCAA3090.11216744530475786No Hit
CATGCCGGGATAATTTAAATTGTAAGACATCATTCACATTTATCAATGTTAC3090.11216744530475786No Hit
AATAGAGGGTACATTTAAACATTAATATTTAAAAGCAGTATTTCATTCTTAA3060.1110784409814107No Hit
CACCTTGGGCATCATTCAGGGATCGTCAGCGTTCACCTATTGCACTTCCTGT3060.1110784409814107No Hit
GCAAGACTTAAAAAATTCCACTTCTAATTCCCCACCTGCATGGAGGGCACAA3050.11071543954029497No Hit
ACAAACGGGATGTCTGGAAAAAATATCCTTCAAGCATGAAGGAGAAAGAAAG3000.10890043233471637No Hit
GCATTAGGGAGGGTGCGCGGGTGCAGTATATCTCGCGCTCTCTCCCCTTTCC2990.10853743089360064No Hit
AGCGATGGGATAGCCAAGATTTCATAGTCCTTTAAATGAAACTAGTGTACTC2940.10672242368802204No Hit
ATTGCAGGGCCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCCG2940.10672242368802204No Hit
GATCATGGGGGAGTTCCACTGTGGGCAAAATTCTATCAAACAGCATCATACA2940.10672242368802204No Hit
GTGTTGGGGCATAGCTCTAACATCGATTTGACTTTTAAAATTGTGCTTACTT2940.10672242368802204No Hit
TGCAGAGGGGAATATTTTTAAAATGCTGACCAAATGCCTTTATGTTTTTATA2930.10635942224690634No Hit
CTTTCTGGGCGATCTTAAGCTGGGGCATTCCTAAAATTATTTTCTACTGACC2930.10635942224690634No Hit
GCGCTGCGGGAACATAGAAAAAACATTTGACAAAATCTAACACCCATTCATA2910.10563341936467488No Hit
AACTTGGGAGCAGGGAAGTAAAATAATTATGGGAAGAGTGTCTTAAGCCTAA2910.10563341936467488No Hit
ACAGTCGGGATGACTCCACATGAACCTTCACATTTGTTCGCTCATAATCTAC2910.10563341936467488No Hit
TTGTTAGGGGCTCCATATCAGATGCTTGATATGGTGCATTGCGGAGGACATA2910.10563341936467488No Hit
TCCATGGGGAAGGGACAATGGAGAGTCAGAAACAATTCTCTCCAATTTGGCT2890.10490741648244344No Hit
AACGCTGGGTAAAGCAACTGGAAAACAAACAAACACAAAGTTCACAAGAGAA2880.1045444150413277No Hit
CTATGACGGGTGTTTGTAAAGCTGACATTTTAGCTGAAAAATCCTTCTGGCC2850.10345541071798055No Hit
TTCTGCGGGAACTGAAATGATTTTTCTTCCAACATTTTATGTTGAAAAATTT2850.10345541071798055No Hit
AACTTGGGGATTCCACCGACGTGGAGGCAGCCGTCAACAGCCTGGTCAATTT2850.10345541071798055No Hit
GCTGTTGGGGGGGCAACCTCCAAGATTCACAACCCAGGAAAAGTACTGCTTT2850.10345541071798055No Hit
TAACTGGGGATGGGCACTATCACTACAGCTATGGACTCAAGCAACTTCTTAA2850.10345541071798055No Hit
ATTGTCGGGGGATCAGTTTGGCTGAATAGATAGATATACTCATGTATGTAGT2810.10200340495351767No Hit
TTAGTAGGGTATCAAATGTGTTAAAAGTGCTTAACCCACTGCCAAATATTCT2800.10164040351240194No Hit
CAAACCGGGGCATATGGATGGATTGGGGATGATTTGCCATGAGTGTATGGTT2780.1009144006301705No Hit
AAACGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTC2780.1009144006301705No Hit
GCTTCGGGGGAGGTAATTTGACACTGCTGCTGGCAGTAGTTCTCTATTCACC2760.10018839774793907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)