FastQCFastQC Report
Sat 31 Dec 2022
SRR5844692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences276567
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC10780.38977896856819505No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9710.3510903325414818RNA PCR Primer, Index 19 (95% over 22bp)
GCAAGACTTAAAAAATTCCACTTCTAATTCCCCACCTGCATGGAGGGCACAA3550.12835949335965607No Hit
TTGTTAGGGGCTCCATATCAGATGCTTGATATGGTGCATTGCGGAGGACATA3420.12365900487042922No Hit
CACCTTGGGCATCATTCAGGGATCGTCAGCGTTCACCTATTGCACTTCCTGT3380.1222127007198979No Hit
CGTTAAGGGTACTAGAATTAATGAACTAAAAATTTAAAAGTTACTTTAGCAA3360.12148954864463221No Hit
ATTGTCGGGGGATCAGTTTGGCTGAATAGATAGATATACTCATGTATGTAGT3340.12076639656936655No Hit
TGTACCGGGTACCAAGTATTAATAAGTCAAAAATTCTTTTGTGAAATGTATC3230.11678906015540537No Hit
TGCAGAGGGGAATATTTTTAAAATGCTGACCAAATGCCTTTATGTTTTTATA3230.11678906015540537No Hit
CTTTCTGGGCGATCTTAAGCTGGGGCATTCCTAAAATTATTTTCTACTGACC3220.11642748411777254No Hit
CTATGACGGGTGTTTGTAAAGCTGACATTTTAGCTGAAAAATCCTTCTGGCC3050.11028069147801438No Hit
TTATTTGGGAACTTGAAGATTGGTTCTCTAAAATAAGATGGACAAAAATAAA3000.1084728112898502No Hit
GATACTGGGACTAAAAGACCTTCTGGATGAAGTAATTTTACTAATTTATTCC2980.10774965921458451No Hit
ACATGAGGGATTGTATCTATGATAATTCAGTTACCCACAATACCCTTTTAAA2970.10738808317695168No Hit
GATGGATTAATGCCCTCTGAAAGATGTTAAAAATGTGAAACATCACTCCTGC2960.10702650713931885No Hit
GATCATGGGGGAGTTCCACTGTGGGCAAAATTCTATCAAACAGCATCATACA2910.10521862695115469No Hit
ATGTTTGGGGACCTAGATCAAATAGATATCTACAGAACTCTCCACCCAAAAA2900.10485705091352185No Hit
ACAACGGGTCTAATGGAACTTTAAAAAATCCATCTCAAAATATTACAGGGAT2880.10413389883825619No Hit
GATGGAGGGGCTCCTCAGAAGTCGCTTAGCTCTTCGGTGGTTGTCACACGTC2870.10377232280062336No Hit
AAACGGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTC2860.10341074676299053No Hit
GCATTAGGGAGGGTGCGCGGGTGCAGTATATCTCGCGCTCTCTCCCCTTTCC2850.10304917072535769No Hit
ACAGTCGGGATGACTCCACATGAACCTTCACATTTGTTCGCTCATAATCTAC2850.10304917072535769No Hit
AACGCTGGGTAAAGCAACTGGAAAACAAACAAACACAAAGTTCACAAGAGAA2810.10160286657482635No Hit
TCACTGGGGAGGATCACATGCGTGAAGCAGGTGATGTATGTTATGCTGATGT2800.10124129053719352No Hit
GGTTATGGGCTGTTAAGAAAAGGGAGTGTTTATGTGTTTGTTTCCAAATATT2800.10124129053719352No Hit
TTTCAAGGGGTAAACAAAATCTATTGCTTCCATCATCATTCTCAGTTTAAAT2780.10051813846192785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)