FastQCFastQC Report
Sat 31 Dec 2022
SRR5844689.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844689.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280374
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT8600.30673314929344375RNA PCR Primer, Index 19 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC8380.29888648733477424No Hit
TGTTCGTGGGACTGTAAGCACCCAGGTTCAAGTCACTGTCCTCTGGTGCCTT3190.11377659840070764No Hit
TTCAGCGAGAGAAAAAAGATTTGATTATCATCACAGCAGAAAAAAGTCATTG2890.10307660482070377No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCTTATAC2860.10200660546270338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)