FastQCFastQC Report
Sat 31 Dec 2022
SRR5844687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224370
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC5280.2353255782858671No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT5050.22507465347417216RNA PCR Primer, Index 19 (95% over 22bp)
GTTCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA3260.14529571689619825No Hit
ATCTGCGGGCACCCCAGAACACCACATTGGCTCCACAACACAAGGAACTCAC2520.11231448054552746No Hit
GCCAGAAAAACATTGAAATGCAGCTGCAAGCCATTCGAATAATTCAAGAGAG2460.10964032624682443No Hit
AACAAAGGGAGAATCCCACAGAAGCAGAGTTACAGGACATGATTAATGAAGT2360.10518340241565272No Hit
GGACAAGGGGGCTGAAATATTTTGGGGACTTTGAAAAAAGAATTCCTCGTGA2350.10473771003253554No Hit
TAACAGGGGGGGAGCCGGGCCGGGGCGATGTGGAGCGCGGGCCGCGGCGGGG2300.10250924811694968No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)