FastQCFastQC Report
Sat 31 Dec 2022
SRR5844673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175966
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC10480.5955695986724708No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9590.5449916461134537RNA PCR Primer, Index 19 (95% over 22bp)
TTTTCTGGGAATGAGAAGTTGCCACTGCAGGGCTAACTCGCCTTCAGAAATA2680.15230214927883795No Hit
GTCTGCGGGATCTTGTGATTAAATTATAGACCAAAATGGCTTAAAAATAGTA2500.1420729004466772No Hit
GTTATGGGAGAGGGAGTGGTGGAAAGAAACAATAGGATGGGAAAAAATGAAA2490.1415046088448905No Hit
CTATGAGGGGACTCCCACACAATAATAATGGGAGACTTTAACACCCCACTGT2360.13411681802166328No Hit
TAGATGGGGCTGGAAAAAGAAGAAGAAAAGAATGGGGCTAGTGTGAAATCTT2330.13241194321630315No Hit
CTTAGCAAGGGGAGTGTGACTCAGAAGGTCCTCAGAGCTGGTAAAAAGTGTA2290.1301387768091563No Hit
GTCGCCGGGATGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAATT2280.1295704852073696No Hit
CTCACTGGCAAGGAAACAAGGCAAATACAGGAGGAAAAAGGTAGGCTGCTGT2270.1290021936055829No Hit
CATCACGGGATATCACACAAATTCAAATGAAATTATCTTACTACATGTTAGA2250.12786561040200947No Hit
TTTTATGGGGTGGGTTTTTCATTCGATTTTACAAAATAGGGAAAGGCACTTC2240.12729731880022277No Hit
TCTTCGATGGGACTTCAGAATATATCTTAGTTTCCCCTTTCCCAGTTTAACA2230.12672902719843604No Hit
TCAATAGGGACCTATGTCCACTGAAAGTTTAAAGCATAGCAACAATTCTACG2220.12616073559664936No Hit
ATGTTTGGGTGATCAGAAGCAGGTAGCCAATGGGAAGAGCACTTCTGAGTGA2210.12559244399486263No Hit
CTTGCCGGGGACGGATAATGAAAACGCTGCCACCCCAGAGAGGGTAAACCCA2180.12388756918950251No Hit
CGTTGCGGGGAGGCTCGGGAGCCGGGAGGCTGCGGGCGGCCGCTGGACCCGA2170.1233192775877158No Hit
CGCTGAGGGGTAATTATTTCTGTTTCTGTATTATTCTGCCCTTAACCAATTC2160.12275098598592911No Hit
CAGTCCGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC2140.12161440278235568No Hit
TCGTATAGGGGGCAGCCATGAGGTGAGGGCGAGCTGGTCTCCATCAGGCGCT2130.12104611118056899No Hit
TACTCAGGGACAAACAAATGGAAAAACATTCCATACTCATGGATAGGAAGAA2130.12104611118056899No Hit
TTTCTGGGGATATCAGTTTTGGTATCTTCCTCTCTAGAGCATCATTTGAACA2110.11990952797699556No Hit
TGCACTGGGATGGAACGCTTTCATAAACTTCAGGGATCCCGTGTTGCCATGG2110.11990952797699556No Hit
TCTAAAGGTGGGGGGCCCAACGCGTTGACCTCGCGGTCAGGTTGCTTCCGCG2110.11990952797699556No Hit
GTTAATAGGAAGATTGTAGTGGTAACTGCAGGAGTCCGTCAGCAAGAAGGGG2090.11877294477342215No Hit
GACGTAGGGACTAAGGGTGCCTTTTAGGAGAAATAAAGAGGAAGATTTGCAA2080.11820465317163543No Hit
CGAATGGGGGGGGGCCAGCCGCCGCCATGATCCTGCTGGAGGTGAACAACCG2080.11820465317163543No Hit
CAAAATGGGATTCTCACTGGGCGAGAGAAATGGATAGGGAATGATAATGATG2040.11593148676448858No Hit
AAAAAAGGGGAATAATATCTTAAATTATTAAAAGAAAACAATGTCTAGCTTC2000.11365832035734175No Hit
TTCATGCGGGGATTCAACCAGCTCATTTTGGGGAGGAGAGAAAATATTCTTA2000.11365832035734175No Hit
TGTAGCGGGTTCCAAACTTGCATTATTCATGAACAACCTTCATGACTTATAT1990.11309002875555506No Hit
TATTAAGGGTCGAGCAGGGGTTGTCAAACTTGGCAATGGCTAAAAAAATAAG1990.11309002875555506No Hit
ATTATAGGGATAATTTTCACATTTAGGAGGCAATAGCAAATCTGGGAAGCAG1990.11309002875555506No Hit
CGTACCAGGGAGAAACAAATGAGAGCAAAATGTTAGGTCCTTTGACAAGCAT1980.11252173715376834No Hit
TTAATGGGGGAGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCG1980.11252173715376834No Hit
TTGTCTGGGGTTTCATTTACTTTCCAAATAGCAAATAAACCAAGTTCTACTG1970.11195344555198163No Hit
ATACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1970.11195344555198163No Hit
GCTGCAGGGGGAAAATGAAAAAGCATCATAAAATACCATGATTTTGTGGGCT1970.11195344555198163No Hit
TAAGATGGGGTGGCAAGCTATACCGGAAAAGCGGCATGTCAAAAATACTTTA1970.11195344555198163No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCTTATAC1970.11195344555198163No Hit
TTATGAGGGAGGCCAGCCTGTCACGTGGGAGGGAGGCTCGGCGCTCAGGAAG1970.11195344555198163No Hit
ATTGTCGGGCTGAGCCTAGATTTGCCAGGGTCTTGGTGCCACGCAAATGGTT1960.11138515395019494No Hit
TTGCTTGGGATTGATAACAATCTCTGTTTTATAGATGACCTAGTATTTTTCA1960.11138515395019494No Hit
TAACGAGGGTAGCTGTGAACCTGCACTTCTCTAAAAAATAAAATTAGTTAAA1940.1102485707466215No Hit
CCGTGAGAGCCTCAACGTGGACGTGGTCAAGTACGAGAGTGGCCCTGACGGA1940.1102485707466215No Hit
TCGTCGGGGCGTCTTTCTGGGCTAGGGATGGGGAACAGAAAGGGAGTATGGA1940.1102485707466215No Hit
TAGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1930.1096802791448348No Hit
AAGAATGGGAAACAAGATAGAGATGTTTTAAAGGCTTCTGGGTTTGATTTCT1930.1096802791448348No Hit
TTAGGGGAATTGTGGTTGGATTTCATCCCTTACTGGTCTCAACCAAAATATT1920.10911198754304809No Hit
GATTGAGGGAATGAAAGATCAGGGAAAATATCTGCAATGTATTTTCCAGATA1920.10911198754304809No Hit
CACCTGGGGCATAGTTTGACATTTTTCTGGCACATTAAATTTAAGAGAATTT1910.10854369594126137No Hit
TCACTCGGGGACGCTCTTCCGATCTTCTTTCGGGCAGTGATGTGTGAGCTAT1910.10854369594126137No Hit
TCATCTGGGGGCTTATAAGTGAGAACATGCGTTATTTGGTTTTCTGTTCCTG1910.10854369594126137No Hit
TACACGGGGGCCTTATGAAGGTTGTATTTGCAGCATCAGGTAGAGTTCAGCT1910.10854369594126137No Hit
ATAACAGGGGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGCCAGACTTTCT1910.10854369594126137No Hit
TGCGATGGGGGCGGGTGCAGGTGGCGCGCTATGGCCGCCGGGACCTGCCCCG1900.10797540433947467No Hit
CTTAGTGGGATCATTTGGCATTCTAAAGGAAGAACGGCTGAAGTAAACCACA1890.10740711273768797No Hit
AGCTTCGGGGAATTAAAATTGTCCTGGAAGACATCTTTACTTTATGGAGACA1890.10740711273768797No Hit
TGGCTAGGACGGCAACCTCCTTTGCCAGGAACTATTTATAAACATCCTGCAG1880.10683882113590125No Hit
TTCAGTGGGCTTGGTTGATATTTGAGTTTTAAAAGGGTCAAATCTTTCTAAT1880.10683882113590125No Hit
TCGCTGGGGTGGAAACTAAGACATTGGACTGAAGGAACTGGCCTGAGGGGGG1880.10683882113590125No Hit
GGTTTGGGGCATTATTTAACATTTAACATTTACAGTGCATCAGGCAGTGTGG1880.10683882113590125No Hit
CATTTAGGGTGGAAAATGCCACATGGGATAGATGACGGATTAGAATGTATAC1880.10683882113590125No Hit
GTCTCAGGGATTGGAGAATATATTAATCAGAAGAAACGTGAGAGATCTGAAG1880.10683882113590125No Hit
ATACGCGGGCATGGAACTGCCCAAACTAAAGCTTTCGGGAGTGGTCAGACTG1870.10627052953411453No Hit
AACATCGGGCTGAGGAAATGTGTTGTACAAAAGCACCAAATAAGGGCACCCT1870.10627052953411453No Hit
AAGGATAGGGATAACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCA1840.10456565472875441No Hit
CTCATAGGGACTGCAGGTTGTGGGTCAAAGTTCTATTTATATATATATTCTG1840.10456565472875441No Hit
AAGCTAGGGGTCTTGGAGGCTTAAATTGTAAAATACATCCCTTATGGAATCC1840.10456565472875441No Hit
TAAGCAGGGCAGGATGGTCTAATAACATGAAAACAGGCTAGTTTCAAGCAAC1840.10456565472875441No Hit
TACGCTGGGAGAACTAATCATATTTTATATCTTCTTCGAAACCACACTTATC1840.10456565472875441No Hit
TTCTGTGGGGGGCCCGGCCGGGGGGGCAAGATGGCGGCGGCAGTAGGGGTTC1840.10456565472875441No Hit
GGCTGCGGGATTTAAAAAGAAGATTATTATTATTTTTTTCTTTCAGCAAATA1830.10399736312696772No Hit
TATCTTGGGCCGCCAACAGAATTCATACGAGAAGGCGACGATGACCGGACTG1830.10399736312696772No Hit
CTAGAAGGGGAGGAAGGCCAATCAGAAATAGCACTGGGACACTAAAGCGTTG1830.10399736312696772No Hit
AGCTGTGGGAGGGTTGTTTAAAGAAAAATCTGTAAATGACTGCAAAGTGACT1830.10399736312696772No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCT1820.10342907152518101No Hit
TTAAATGGGGTTGTCTGGGAACTTTAGGATCCTCTAGGAAGCTAATCTGCTT1820.10342907152518101No Hit
TTTATTGGGCGACCTTTACATCTATTTTATTTGCAGGTCATATATCTAATAA1820.10342907152518101No Hit
TAGAAAGGGATACATCCTCACTTTCTTCCGCAGTTTACCCTGGTTTTCCTCA1810.10286077992339429No Hit
GTCTTTGGGGTTCTCAAGGAAGATTTAACTCTCTTTGGTTTTAAATTACTTT1810.10286077992339429No Hit
TGCCACGGGGAGATGTGCTTGAAAGAAAAAAGCTGCTACCCCTATTTTTTAA1810.10286077992339429No Hit
AACCTGGGGCGTGTGGGGCAGTGTCCATCCCCTGCAGCTACTTGGTGACTTA1810.10286077992339429No Hit
CAAAACGGGGTCTGACATTTTAGCCAGTCAACTTGGAGCACTGAATATATTT1810.10286077992339429No Hit
CAAGGTGGGCCTGCCCACACCCTCTGGGCTGAGTTCCTTCTTTCCCTTGGAC1810.10286077992339429No Hit
GAGCATGGACTTAAATGTAAAATGTAAAACTTTATAAAAAGTGTAAAGAAAA1810.10286077992339429No Hit
CACCGTGGGAGAGACTATAGAAATAAACCCATAAAAATATGCCCAACTGACA1800.10229248832160759No Hit
AAAAAAGGGGTGCAGGAACTGTGGTCATTGTTCATGTTTGGATGTATTACAA1800.10229248832160759No Hit
ATTGCAGGGCCTATATGAAATTTACCAAATTAGACCAACAGTTAAGTGTAAT1800.10229248832160759No Hit
AAACGCGGGATTCCATTATTAAATTCTGCCCTATTCCCAATTGTTAGCATCT1800.10229248832160759No Hit
TGAAAGGGGAGACCAAGAGGAAGAGGCAGGTCCCAGCTGTGACAAGCATGTC1800.10229248832160759No Hit
AGCTCTGGGATTGCCTGTTGGTCCTGCAGGCGGAAACCAAATGTTGGAGCAG1800.10229248832160759No Hit
AAAAAGGGGGTATTTAGTCTTTCCATAAAATTTAAAAAGCCAAGAATGAACT1800.10229248832160759No Hit
GTGCAGGGGTAATAATAATAATAATAAAAGCAGTTGGTTCAGTGATTCTGAA1790.10172419671982087No Hit
GGCTATGGGGGTGGGGATTGAAAATCTCCAGATTTATTTCATGTATATCACA1790.10172419671982087No Hit
ATTCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1790.10172419671982087No Hit
AAAGTAGGGAACTAGAATTAGAATTAGGGCCTTAAGGTGAGATGAAATTGCT1790.10172419671982087No Hit
GCTGACGGGGGTCTACGCCGAGTGACAGAGACGCTCAGGCTGTGTTCTCAGG1790.10172419671982087No Hit
AAATTAGGGGGAAATGGAGGGGTGTGTGTCTAACCTAATGGTCTGCAACCTG1790.10172419671982087No Hit
TATGGTGGGCGAGAAGGGATTTCTTAAATATTTTTAACTTCTAATTTGTATT1780.10115590511803416No Hit
CAGCATGGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGCGCTGCTGCT1780.10115590511803416No Hit
TTACAGGGAGGCCTGTCTACACTTGCTGTCTTCCTTCCTCACCTCCAATTTC1770.10058761351624745No Hit
AAAGGCGGGTTGTTTCAGCTGTTCTGTAGGCTGCATAACCACTCTGATATTT1770.10058761351624745No Hit
TACTTAGGGGTGGAGCAGAGGAAGCTGCTGGAAAAGCGGTCCCTGCATGATG1760.10001932191446075No Hit
ACGGTGGGGGAGTCACGCGCGCCGGACGCCACCCGACAGGAGGGGAAACGGG1760.10001932191446075No Hit
ATGGCTGGGAAGAGGGGGATCTATGAGGGGTTGAGTGATTTGCAGCTTTTAA1760.10001932191446075No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)