FastQCFastQC Report
Sat 31 Dec 2022
SRR5844672.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844672.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174477
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC10990.6298824486895122No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9730.5576666265467655RNA PCR Primer, Index 19 (95% over 22bp)
TTTTCTGGGAATGAGAAGTTGCCACTGCAGGGCTAACTCGCCTTCAGAAATA2750.15761389753377236No Hit
CTATGAGGGGACTCCCACACAATAATAATGGGAGACTTTAACACCCCACTGT2630.1507362001868441No Hit
CGTTGCGGGGAGGCTCGGGAGCCGGGAGGCTGCGGGCGGCCGCTGGACCCGA2430.13927337127529704No Hit
TCTAAAGGTGGGGGGCCCAACGCGTTGACCTCGCGGTCAGGTTGCTTCCGCG2410.1381270883841423No Hit
GTTATGGGAGAGGGAGTGGTGGAAAGAAACAATAGGATGGGAAAAAATGAAA2400.13755394693856499No Hit
TCACTCGGGGACGCTCTTCCGATCTTCTTTCGGGCAGTGATGTGTGAGCTAT2360.13526138115625555No Hit
CATCACGGGATATCACACAAATTCAAATGAAATTATCTTACTACATGTTAGA2330.13354195681952347No Hit
GATTGAGGGAATGAAAGATCAGGGAAAATATCTGCAATGTATTTTCCAGATA2320.13296881537394614No Hit
GTCGCCGGGATGTGTTTGCATAAGGAATGTTAAAATTCTTTCTACTACAATT2310.1323956739283688No Hit
TCGTCGGGGCGTCTTTCTGGGCTAGGGATGGGGAACAGAAAGGGAGTATGGA2290.13124939103721409No Hit
AAGGATAGGGATAACAAGTCTCTGGCAGAGCACCGAACCCCAGATGTGCTCA2250.12895682525490465No Hit
CGAATGGGGGGGGGCCAGCCGCCGCCATGATCCTGCTGGAGGTGAACAACCG2250.12895682525490465No Hit
CTTGCCGGGGACGGATAATGAAAACGCTGCCACCCCAGAGAGGGTAAACCCA2230.12781054236374995No Hit
GGCTATGGGGGTGGGGATTGAAAATCTCCAGATTTATTTCATGTATATCACA2210.12666425947259524No Hit
ATAACAGGGGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGCCAGACTTTCT2210.12666425947259524No Hit
GTCTGCGGGATCTTGTGATTAAATTATAGACCAAAATGGCTTAAAAATAGTA2210.12666425947259524No Hit
TAGATGGGGCTGGAAAAAGAAGAAGAAAAGAATGGGGCTAGTGTGAAATCTT2200.1260911180270179No Hit
TTCATGCGGGGATTCAACCAGCTCATTTTGGGGAGGAGAGAAAATATTCTTA2190.12551797658144054No Hit
CTTAGCAAGGGGAGTGTGACTCAGAAGGTCCTCAGAGCTGGTAAAAAGTGTA2180.12494483513586319No Hit
TTTTATGGGGTGGGTTTTTCATTCGATTTTACAAAATAGGGAAAGGCACTTC2160.12379855224470848No Hit
TGCGATGGGGGCGGGTGCAGGTGGCGCGCTATGGCCGCCGGGACCTGCCCCG2150.12322541079913112No Hit
TTTCTGGGGATATCAGTTTTGGTATCTTCCTCTCTAGAGCATCATTTGAACA2150.12322541079913112No Hit
CAAAACGGGGTCTGACATTTTAGCCAGTCAACTTGGAGCACTGAATATATTT2150.12322541079913112No Hit
CTCACTGGCAAGGAAACAAGGCAAATACAGGAGGAAAAAGGTAGGCTGCTGT2130.1220791279079764No Hit
CGTACCAGGGAGAAACAAATGAGAGCAAAATGTTAGGTCCTTTGACAAGCAT2120.12150598646239906No Hit
GTTAATAGGAAGATTGTAGTGGTAACTGCAGGAGTCCGTCAGCAAGAAGGGG2120.12150598646239906No Hit
TCGCTGGGGTGGAAACTAAGACATTGGACTGAAGGAACTGGCCTGAGGGGGG2120.12150598646239906No Hit
GTCTCAGGGATTGGAGAATATATTAATCAGAAGAAACGTGAGAGATCTGAAG2100.12035970357124434No Hit
TCAATAGGGACCTATGTCCACTGAAAGTTTAAAGCATAGCAACAATTCTACG2090.119786562125667No Hit
CAGTCCGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCC2060.11806713778893492No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCTTATAC2030.11634771345220288No Hit
GACGTAGGGACTAAGGGTGCCTTTTAGGAGAAATAAAGAGGAAGATTTGCAA2030.11634771345220288No Hit
AAGCTAGGGGTCTTGGAGGCTTAAATTGTAAAATACATCCCTTATGGAATCC2010.11520143056104816No Hit
CAGCATGGGAGTCTCTGGGCTCGGTGCCAGCGCCTGCCTGCGCGCTGCTGCT2000.11462828911547082No Hit
CCGTGAGAGCCTCAACGTGGACGTGGTCAAGTACGAGAGTGGCCCTGACGGA2000.11462828911547082No Hit
GCAACAGGGGAATGAGGATGGCCTGACGCCTGAGCAGCGAGTAGACAAGATT2000.11462828911547082No Hit
TCTTCGATGGGACTTCAGAATATATCTTAGTTTCCCCTTTCCCAGTTTAACA1990.11405514766989346No Hit
CAACACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1990.11405514766989346No Hit
ATGGCTGGGAAGAGGGGGATCTATGAGGGGTTGAGTGATTTGCAGCTTTTAA1990.11405514766989346No Hit
TTAATGGGGGAGAAGTGGCCCTTGCAGGCAAGAGTGCTGGAGGGCGGCAGCG1980.1134820062243161No Hit
TTGTCTGGGGTTTCATTTACTTTCCAAATAGCAAATAAACCAAGTTCTACTG1960.1123357233331614No Hit
TACTCAGGGACAAACAAATGGAAAAACATTCCATACTCATGGATAGGAAGAA1960.1123357233331614No Hit
TTGCTTGGGATTGATAACAATCTCTGTTTTATAGATGACCTAGTATTTTTCA1960.1123357233331614No Hit
TGGCTAGGACGGCAACCTCCTTTGCCAGGAACTATTTATAAACATCCTGCAG1950.11176258188758403No Hit
TTAAATGGGGTTGTCTGGGAACTTTAGGATCCTCTAGGAAGCTAATCTGCTT1950.11176258188758403No Hit
AGCTTCGGGGAATTAAAATTGTCCTGGAAGACATCTTTACTTTATGGAGACA1950.11176258188758403No Hit
TAGCCTGGGAATCAGTTTCTACATGTTTAAGTGTTCAGGGCTTCATCAGCAT1940.11118944044200668No Hit
ACGGTGGGGGAGTCACGCGCGCCGGACGCCACCCGACAGGAGGGGAAACGGG1930.11061629899642932No Hit
AGCTGGGGATACATACATAAAATTGATTTTTAAATTTAAAACATATGGAAAA1930.11061629899642932No Hit
CGTTACGGGAATGAATGAATGAATGAGTGATTTTTTTAATTCTCCTTTTTTT1920.11004315755085198No Hit
CACGCAGGGTCGGGACGCGTTGCTGCGCCCGTGCCCCTTGCTTTGGCGCGCA1920.11004315755085198No Hit
GGTTTGGGGCATTATTTAACATTTAACATTTACAGTGCATCAGGCAGTGTGG1920.11004315755085198No Hit
TAGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1910.10947001610527461No Hit
CAAACCGGGAGGAGGAACTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGG1910.10947001610527461No Hit
AAAAAGGGGGTATTTAGTCTTTCCATAAAATTTAAAAAGCCAAGAATGAACT1910.10947001610527461No Hit
GCCACAGGGGGAAACTTTCTTACTCAAGAATGACGGCAGTATTGTTTTCTTA1910.10947001610527461No Hit
ACTTCTGGGTTTACCAAAAGTAGCTATTCTCTGCCAGTGTACTGTTTACAGT1900.10889687465969727No Hit
CTCGTTGGGACCACATGCCTGTGGAGAAAGTGCGACATGTTTAGAAATACTG1900.10889687465969727No Hit
CAGAATGGGTATCCTTTCTGCTCAGAATTTTATACTTATTTTTCAGAATGAG1900.10889687465969727No Hit
TGCACTGGGATGGAACGCTTTCATAAACTTCAGGGATCCCGTGTTGCCATGG1890.10832373321411992No Hit
ATTATAGGGATAATTTTCACATTTAGGAGGCAATAGCAAATCTGGGAAGCAG1890.10832373321411992No Hit
TTCAGTGGGCTTGGTTGATATTTGAGTTTTAAAAGGGTCAAATCTTTCTAAT1880.10775059176854256No Hit
GCTTTCTCCCCACTGGCTGGGCTGCATCTGGCCCTGGCTGGAGGCCTTGCTT1880.10775059176854256No Hit
CACCTGGGGCATAGTTTGACATTTTTCTGGCACATTAAATTTAAGAGAATTT1880.10775059176854256No Hit
TACGCTGGGAGAACTAATCATATTTTATATCTTCTTCGAAACCACACTTATC1880.10775059176854256No Hit
ACTCATGGGGCTACTGGTGTTATGGAAAATCCTTATGAAAAGCTAGAAAATA1870.10717745032296522No Hit
GCAATAGGGGGCCCCTGCCCTGAGGACCCTAGTCCAACATGGCGGCGCCCAG1870.10717745032296522No Hit
CAAAATGGGATTCTCACTGGGCGAGAGAAATGGATAGGGAATGATAATGATG1870.10717745032296522No Hit
GTCACTGGGACCAGCGTAAAGATTAAAGTGGAAAACTTCAGACTTTGGCTTC1870.10717745032296522No Hit
TTTCTAGGGGTTTGCCAACAACCCCAGCCAGAAGTCCAGCCAGGCCCTGCTC1870.10717745032296522No Hit
TGTAATGGGATTGATGAATATTATCGGATGAAGAAGGAGGAAGAAGAAGAAG1870.10717745032296522No Hit
CTTGAAGGGACCTCCCAATCTGATGAAACGATAGAATAAAGTAACATTTCCC1860.10660430887738785No Hit
TACACGGGGGCCTTATGAAGGTTGTATTTGCAGCATCAGGTAGAGTTCAGCT1860.10660430887738785No Hit
ATTGTCGGGCTGAGCCTAGATTTGCCAGGGTCTTGGTGCCACGCAAATGGTT1850.1060311674318105No Hit
AGAGCAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1840.10545802598623313No Hit
TGTATAGGGCTAAGAGAGGTGCACCCTTGAGCCTCTTTTGAACTCTGTTCAA1840.10545802598623313No Hit
CATTTAGGGTGGAAAATGCCACATGGGATAGATGACGGATTAGAATGTATAC1830.1048848845406558No Hit
CTTAATGGGGAATAATCTCAATTATATTCATCACACTTTTTTCATACCATTT1830.1048848845406558No Hit
CACCGTGGGAGAGACTATAGAAATAAACCCATAAAAATATGCCCAACTGACA1820.10431174309507843No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCCTGTCTCT1820.10431174309507843No Hit
TTACAGGGAGGCCTGTCTACACTTGCTGTCTTCCTTCCTCACCTCCAATTTC1820.10431174309507843No Hit
TAAGATGGGGTGGCAAGCTATACCGGAAAAGCGGCATGTCAAAAATACTTTA1820.10431174309507843No Hit
GATGTGGGGACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGTTTACAGCT1820.10431174309507843No Hit
TGCCACGGGGAGATGTGCTTGAAAGAAAAAAGCTGCTACCCCTATTTTTTAA1820.10431174309507843No Hit
ATGTTTGGGTGATCAGAAGCAGGTAGCCAATGGGAAGAGCACTTCTGAGTGA1820.10431174309507843No Hit
TATGGTGGGCGAGAAGGGATTTCTTAAATATTTTTAACTTCTAATTTGTATT1810.10373860164950108No Hit
CATTAAGGGGAAGTATGATTATATTCAGAAACGACTAGCTTTGTAAGTGATA1810.10373860164950108No Hit
CTAGAAGGGGAGGAAGGCCAATCAGAAATAGCACTGGGACACTAAAGCGTTG1810.10373860164950108No Hit
CTAAGAGAGGGTACCTATCTCCTAGTTATGTCATAGCAGTTGTAACATTGTA1810.10373860164950108No Hit
TGTAGCGGGTTCCAAACTTGCATTATTCATGAACAACCTTCATGACTTATAT1800.10316546020392371No Hit
TTAAGAGGGATTTCATTACATTTTTACAGGCTGTCTCAAGGAGCTTTTCTGC1800.10316546020392371No Hit
CCTCGGGCAGCAGAAGTATGGGGATCATTTCATAAAGTCTTTTTGAAAAGTT1800.10316546020392371No Hit
CGCTTGGGGTTCTCACCTATCAGTAAAAACCTTGAATGCAAATGGATTAAAT1800.10316546020392371No Hit
TTCTGTGGGGGGCCCGGCCGGGGGGGCAAGATGGCGGCGGCAGTAGGGGTTC1800.10316546020392371No Hit
AAAAAAGGGGAATAATATCTTAAATTATTAAAAGAAAACAATGTCTAGCTTC1790.10259231875834637No Hit
GCGAATGGGATGAAGGGAGTCATGGGGAATTCCTGAAAATTTTCTTCCTTCT1790.10259231875834637No Hit
TAGTGGGGATGCCAGTGCAGAAGCAGACCCGTGCCGGCCAGCGCACCAGGTT1790.10259231875834637No Hit
GTATCAGGGGTTTGGTTCTTCCCTGAAACTCCCACAACATGGAAATAAACAG1790.10259231875834637No Hit
AACACGGGGACGAGATGATGATGCAGTGTGTGTCCCGCATGTTGGCCCACCC1790.10259231875834637No Hit
ATACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1780.10201917731276902No Hit
AGCTGTGGGAGGGTTGTTTAAAGAAAAATCTGTAAATGACTGCAAAGTGACT1780.10201917731276902No Hit
TGCCTAGGGGACTGACGCACTCTGCGCCCGGAGGACAGAGCGGCCCGGTCGC1780.10201917731276902No Hit
TGGATCAGGGAGGTCGTTCACATGTCTAGTGCCTGGTCTGGCATAGCTTGAA1770.10144603586719166No Hit
AAAAAAGGGGTGCAGGAACTGTGGTCATTGTTCATGTTTGGATGTATTACAA1770.10144603586719166No Hit
TTATGAGGGAGGCCAGCCTGTCACGTGGGAGGGAGGCTCGGCGCTCAGGAAG1770.10144603586719166No Hit
TCATCTGGGGGCTTATAAGTGAGAACATGCGTTATTTGGTTTTCTGTTCCTG1770.10144603586719166No Hit
TAATTTGGGGATAGGATTTTAGTCTCTTACTATGAATACTAATTTCGAACCA1760.10087289442161432No Hit
TGAAGCGGGCGTATGGGACCTACACTTAATCTATATGCTTTACACTAGCTTT1760.10087289442161432No Hit
TTCAATGGGAGACCAAGCGCAGCATTCAGTTTGTGGACTGGTGCCCCACAGG1760.10087289442161432No Hit
CTCAGTGGGGATTAAGTGCTAATCAACAAAATATTCTGAAATTCATTGCTTG1760.10087289442161432No Hit
GTTGTCGGGCTATGGGACCAGTAAATTACAGATTTTTTAGGTTTTAAATCAG1760.10087289442161432No Hit
AGATAGGGGACAACAGTTGCTTTCATAAAGACTAGTTCTTATCAACCTTGAA1760.10087289442161432No Hit
GGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCA1750.10029975297603695No Hit
TAACGAGGGTAGCTGTGAACCTGCACTTCTCTAAAAAATAAAATTAGTTAAA1750.10029975297603695No Hit
AAGAATGGGAAACAAGATAGAGATGTTTTAAAGGCTTCTGGGTTTGATTTCT1750.10029975297603695No Hit
TAAGCAGGGCAGGATGGTCTAATAACATGAAAACAGGCTAGTTTCAAGCAAC1750.10029975297603695No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)