FastQCFastQC Report
Sat 31 Dec 2022
SRR5844664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230223
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC10510.4565139017387489No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9800.4256742375870352RNA PCR Primer, Index 19 (95% over 22bp)
TACTGTGGGTGGTGCTGCCCAGAAAGCTTTGTTGGAAAAAGATTTGAAGCAA3770.16375427303093087No Hit
CGCTTGGGGGGAGAAGCGTGCCTCAGGCCAGGCTTTTGAGCTGATTCTCAGC3230.1402987538169514No Hit
TGAAACCGGGCCAGAAACTACTGGCAGAAGGAAAGAGTACTAGAATTCAGAT3130.1359551391476959No Hit
TAAGCAGGGAGCTGCCCGGAGTGCGCCCGTGTCTTTGCCAGCCCTCTGCGGC3110.13508641621384482No Hit
CAAAGTGGGCACTTCTCTGAATGTGTGATCTCATCACTAAAATGGAATAATG2970.1290053556768872No Hit
TGAATGGGGATTGATTTTCTGTAACTGATAAATCATCATGAAAGTAAGTGTT2870.12466174100763174No Hit
TCACGAGGGATAAGTAGACATTCTGATTTCTTTTAAAATAAACTAGCAGCCT2830.12292429513992954No Hit
CAGCACGGGTGTTTTGTCTTCCCACAGAGCTCAACAAAGGAGCCGGTTGCCC2810.12205557220607846No Hit
TACGAAGGGAGCCTGGAGCACCTTTCCTTCAAGCCAGCAACACAGAGCACTA2810.12205557220607846No Hit
TTATTTGGGGGGCGCGCGGGTGCGGGGACTACCCGCCAGGCTCCGGCTCGGG2720.11814631900374853No Hit
ACAACGGGAGTTCAATTGGCTACCGGAAAAAACCAGGCTGGGCTGGGCGCCG2720.11814631900374853No Hit
AAGCCAGGGATATTCACAAATTTGCCAAAGCCCAGTTAACTTCAATTCTTCG2690.1168432346029719No Hit
ATATCTGGGATCTAGCCTAAACAAAATTGCTGACACAAAATCCAGCATCGAC2670.11597451166912082No Hit
ACGATTGGGGGATGTAAATATTTAAATATGGGAGAAAAAATGTTTTTTGAAT2670.11597451166912082No Hit
ATAATGGGGATATCAGAACGTCCTGTAATCATTTAGTTAGAAACTAACTACT2660.11554015020219525No Hit
TAGCGCGGGACAGTGATTGAGGAGCACCTGGGGAAGTTTGGCGTCATTTGCT2650.11510578873526972No Hit
AAAAGCGGGGCTTCTTTTTTAATTTGCTCTTACAATGAAAATACTAATAAAA2560.1111965355329398No Hit
GAATATGGGACAGATACGTGATATTTTGGTATAACTAGAATCTTGATTTCTT2560.1111965355329398No Hit
CATAGAGGGAAATAGAAAGAAGAAAGCCTGTAATCTGTTTCAGAATGCTATT2550.11076217406601427No Hit
TGTACGGGAAGCCACACCCACTCAGACACCCCTCACCTCTCATCTACTTAGA2540.1103278125990887No Hit
CGCCCGGGAAATGGTCCTGGAGCAGTCAGATATTCATAAGGAAAAACAATCA2540.1103278125990887No Hit
CAATAAGGGAATGATGGCAACCATTAAAATGATAGAAACCACTTACTAATCA2530.10989345113216317No Hit
GTAAATGGGGAGCTCATTTCTACCAATATATTACTATATTAACTGGAACATT2530.10989345113216317No Hit
TATTCAGGCAGGCAGGGCCAGGCCTTGGCCCCACTGGAGGTAGAGCACCTTC2530.10989345113216317No Hit
CAGCAGGGGGTAAGAAATTTGGTTTGTTTTAACCATTTATAGGTAGGAAATT2520.10945908966523761No Hit
ACACTAGGGCAAGAGATTTTGAACTATCCAATTATGACTGCTATGGAAAACC2500.10859036673138653No Hit
ATCCGGGGAGTTCTTCATACCTGTACAAAGATTACCCAGGATCACTTCATTC2500.10859036673138653No Hit
GGACTTGGGTCAGGGTCTGAGGAGGCTGTGACGCCCTATGACCGCAGAGATC2500.10859036673138653No Hit
CAATTCGGGGCACGATCTCGGCCCACCGTAACCTCCACCTCCTGGGTTCAAA2500.10859036673138653No Hit
TCAAGACGGGAGGAGAGATTAGTCAACTGTGACAGATACTACTAAAGGGAGA2490.108156005264461No Hit
TTATGGGGGAAACTGCGTCTCACAAAAAAGAAAAAAATTGAATAGCCCTATA2460.10685292086368434No Hit
GATTTGGGCAGTAATGAGTGGATGAAAACATTTTAAGTAATCTTCTACTATT2450.1064185593967588No Hit
TTGTGGGGGCCTAAAACAAGCTGAACATCAAAAAATTGACTGTAATGGATGA2450.1064185593967588No Hit
AAGATTGGGCAAGTGCCTCAAGCATTACTGCTCACCACTCAGCTACCGCCAG2440.10598419792983325No Hit
TGTTCTGGGCAGTTGCAAAAGGCCACATATTGTGTGCTTCTATTTATGTGAA2440.10598419792983325No Hit
ACAAATGGGACTTCAAACACACCTGTAATCTGCTTTTTGCACAAAGTAAAAG2440.10598419792983325No Hit
AGCGCAGGGGTTTCTTACAAAACCAGACATTCTTACCATATGATCTAGCAGT2420.10511547499598216No Hit
TGGCTAAGGGGCAGGAAGGGAACTCCACCACTCAGGGGCAAGCTCGGGAAAA2410.1046811135290566No Hit
TATAACGGGATGCATCAGATGCTGGATCAGACTTTACTGGAGTTAAACAACA2410.1046811135290566No Hit
AGCATAGGACATACTTTTATTGAAAATATCTTTTCAGCTAAAACTCATGGCC2400.10424675206213106No Hit
TCCGACGGGTGCCCTTTGATGCTTGATTCCAGTGATTGATTCCCTTTGTTGC2400.10424675206213106No Hit
CTCAACAGGGGGGGCCATCTTGCTTTCTAAACATAGCACCTTCCTGACTGTA2400.10424675206213106No Hit
CGAAGAGGGTGAATGGAAACTTGGAAATACTCGTTTTTATAAACTACAAAAA2400.10424675206213106No Hit
ACCAACGGTGGCAAGGATGTGAGAACAACTAGAACCTGTTACTGGTGGGATT2390.10381239059520551No Hit
TTGTATGGGCTTTGTTTTATATAATGCATTCTGCATTTTTAAGAAGTGTTCA2390.10381239059520551No Hit
GCTGCAGGGATTATGAACAAGTTTACAACAACCAATTTGGCAACTTAGATGA2380.10337802912827998No Hit
GCACTAGGGCATATTCATAGTAGGAAATTTAGAAAATAGAAAATGATAAAAA2380.10337802912827998No Hit
ACGAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2380.10337802912827998No Hit
CGCTGCGGGATCCTAGAATAGCAAATCTGGTGAAAATACATTTCAATCATGA2380.10337802912827998No Hit
CGCGGGGATCACAATGATAAAGGAATCAATTCAACAAGAAGAGCTAACTATC2370.10294366766135442No Hit
CACAGCGGGATTAGTTTATATTTTAAAGGTAGTGTATTAGTTAGCTCACATT2370.10294366766135442No Hit
AAGCCAGGGCTTTAGCTCAAGAGGCATTGAAAGGAAAGAACCAAGAGGAACA2360.10250930619442888No Hit
ACATCTGGGGGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATT2340.10164058326057779No Hit
CGTGGCGGGAGACTATTGCTCACTGCAAACACTTTGAGAGAAGAATCAAAAT2330.10120622179365224No Hit
GGTTATGGGGGAGGGAGAAATGAAATAAGATCACTAGAAGGGTGGATGCTTT2320.10077186032672669No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)