FastQCFastQC Report
Sat 31 Dec 2022
SRR5844663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229645
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC10170.44285745389623116No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9290.40453743821986116RNA PCR Primer, Index 19 (95% over 22bp)
TGAAACCGGGCCAGAAACTACTGGCAGAAGGAAAGAGTACTAGAATTCAGAT3450.1502318796403144No Hit
TAGCGCGGGACAGTGATTGAGGAGCACCTGGGGAAGTTTGGCGTCATTTGCT3250.1415227851684121No Hit
CGCTTGGGGGGAGAAGCGTGCCTCAGGCCAGGCTTTTGAGCTGATTCTCAGC3210.13978096627403164No Hit
TACTGTGGGTGGTGCTGCCCAGAAAGCTTTGTTGGAAAAAGATTTGAAGCAA3180.1384746021032463No Hit
CAAAGTGGGCACTTCTCTGAATGTGTGATCTCATCACTAAAATGGAATAATG3170.1380391473796512No Hit
TAAGCAGGGAGCTGCCCGGAGTGCGCCCGTGTCTTTGCCAGCCCTCTGCGGC3140.13673278320886587No Hit
TGAATGGGGATTGATTTTCTGTAACTGATAAATCATCATGAAAGTAAGTGTT2970.1293300529077489No Hit
TCACGAGGGATAAGTAGACATTCTGATTTCTTTTAAAATAAACTAGCAGCCT2930.12758823401336847No Hit
AAAAGCGGGGCTTCTTTTTTAATTTGCTCTTACAATGAAAATACTAATAAAA2810.12236277733022709No Hit
ATATCTGGGATCTAGCCTAAACAAAATTGCTGACACAAAATCCAGCATCGAC2730.11887913954146617No Hit
GATTTGGGCAGTAATGAGTGGATGAAAACATTTTAAGTAATCTTCTACTATT2670.11626641119989549No Hit
TGAGTAGGGGGTTGTGGGCATTGTTTTCTACAACCAAATTCTGGGTTTTTTT2670.11626641119989549No Hit
TCCGACGGGTGCCCTTTGATGCTTGATTCCAGTGATTGATTCCCTTTGTTGC2640.11496004702911014No Hit
AAGATTGGGCAAGTGCCTCAAGCATTACTGCTCACCACTCAGCTACCGCCAG2640.11496004702911014No Hit
CAATAAGGGAATGATGGCAACCATTAAAATGATAGAAACCACTTACTAATCA2630.11452459230551504No Hit
AGCATAGGACATACTTTTATTGAAAATATCTTTTCAGCTAAAACTCATGGCC2620.11408913758191991No Hit
GCACTAGGGCATATTCATAGTAGGAAATTTAGAAAATAGAAAATGATAAAAA2610.1136536828583248No Hit
TTCGATGGGAAGATGGGATCTGTTTGTTACCTTGGAGGTAATCACTGTAGGA2610.1136536828583248No Hit
TTAAATGGGAAGGTATGGCACAAGGTAACCTGTAAGCCAGGGTGCCCAGACC2610.1136536828583248No Hit
ATCCGGGGAGTTCTTCATACCTGTACAAAGATTACCCAGGATCACTTCATTC2600.11321822813472968No Hit
TATCAAGGGTATGCCAAATACAGTTTGTCATGAGTAATCCATTTAGGGTACA2600.11321822813472968No Hit
TTATGGGGGAAACTGCGTCTCACAAAAAAGAAAAAAATTGAATAGCCCTATA2570.11191186396394436No Hit
TGTACGGGAAGCCACACCCACTCAGACACCCCTCACCTCTCATCTACTTAGA2560.11147640924034923No Hit
AAGCCAGGGATATTCACAAATTTGCCAAAGCCCAGTTAACTTCAATTCTTCG2560.11147640924034923No Hit
TCATGGGGTAATCCACCATGATCAAGTGGGTTTCATACCAGGGACGCAGGGA2520.10973459034596877No Hit
TTCAGGGGGTATTTTGTGCATTTAGAAACATTATCCTGAGAAGGGGTTCAGA2520.10973459034596877No Hit
ACATCTGGGGGGATAACTTAAACAAAGAAATTGAAAGCACTAAAGGTGAATT2520.10973459034596877No Hit
CCTCGAGGGGTAGACACAAAATTTCTCAATAAAATACTAGCAAACACTGGCA2510.10929913562237367No Hit
GGACTTGGGTCAGGGTCTGAGGAGGCTGTGACGCCCTATGACCGCAGAGATC2490.10842822617518344No Hit
CTCAACAGGGGGGGCCATCTTGCTTTCTAAACATAGCACCTTCCTGACTGTA2480.10799277145158831No Hit
AGGCGTGGGGCTCCGGGTGCTGAATCCAGGCGTGGGGACACGAGCCAGGCGC2470.10755731672799321No Hit
GAATATGGGACAGATACGTGATATTTTGGTATAACTAGAATCTTGATTTCTT2470.10755731672799321No Hit
GTGACTGGGATCTTCTTATGTATTAAAGTGCAATTTCTTTACCACTACTGTC2450.10668640728080299No Hit
CATAGAGGGAAATAGAAAGAAGAAAGCCTGTAATCTGTTTCAGAATGCTATT2450.10668640728080299No Hit
CCTGTGGGGCAGCACTCTAATTGAGATATAAACAGATGTGATTGTAAGAAAC2440.10625095255720786No Hit
TTATTTGGGGGGCGCGCGGGTGCGGGGACTACCCGCCAGGCTCCGGCTCGGG2430.10581549783361276No Hit
ACAACGGGAGTTCAATTGGCTACCGGAAAAAACCAGGCTGGGCTGGGCGCCG2430.10581549783361276No Hit
TTGTATGGGCTTTGTTTTATATAATGCATTCTGCATTTTTAAGAAGTGTTCA2420.10538004311001763No Hit
CAGCAGGGGGTAAGAAATTTGGTTTGTTTTAACCATTTATAGGTAGGAAATT2400.10450913366282741No Hit
TACGAAGGGAGCCTGGAGCACCTTTCCTTCAAGCCAGCAACACAGAGCACTA2390.1040736789392323No Hit
GTACCTGGGTGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCAC2380.10363822421563718No Hit
AGCGCAGGGGTTTCTTACAAAACCAGACATTCTTACCATATGATCTAGCAGT2370.10320276949204207No Hit
GGCTATGGGACAACCATTTACTTGGTGTAAAGAACTTAATTTCAGTATAAAC2370.10320276949204207No Hit
GTTCACGGGACTGTCGGCCCTGCTGCGAGCCCACACGCCCCTCCACATGGCT2370.10320276949204207No Hit
GTGTGTGGGGAAAGGGACATATGAGAAGTCTGTACTCTCTGCTCCGTTTAGC2370.10320276949204207No Hit
CAATTCGGGGCACGATCTCGGCCCACCGTAACCTCCACCTCCTGGGTTCAAA2370.10320276949204207No Hit
AACACTGGGCAGATTGGACATGCTGGGCAACTCATGCTTTGAAACACTTGGA2360.10276731476844694No Hit
ACGATTGGGGGATGTAAATATTTAAATATGGGAGAAAAAATGTTTTTTGAAT2360.10276731476844694No Hit
ACCAACGGTGGCAAGGATGTGAGAACAACTAGAACCTGTTACTGGTGGGATT2350.10233186004485184No Hit
TCGCGGGCGATCTACAGGGGGGGGGCGGGCCTGGAGCTGCACCCGCTTCTGG2350.10233186004485184No Hit
AGCTGCGGGCATGGAGGCCTTTAAACCCTTCCTGGGCATTGGATTAAAAAAT2350.10233186004485184No Hit
TCAAGACGGGAGGAGAGATTAGTCAACTGTGACAGATACTACTAAAGGGAGA2340.10189640532125673No Hit
GTCAACGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG2330.10146095059766161No Hit
ATGTATGGGACAGAGGTTCTTCAAAAAAATTACCATATGATCCAGCAATTCG2330.10146095059766161No Hit
TTAGATGGGTAGGTAAGAACAAATCCTTTGAGATTTTCAAGCGTCCCTCTGG2320.10102549587406649No Hit
GCTATGGGCCTCAGATTTATTTTGTCTTGCCCCATTGCCTCTCCTTCCTTGA2320.10102549587406649No Hit
CATAACGGGCGATCTGTATTAGGGAACCTCTTGTTACTAAATATTCCATTAG2310.10059004115047139No Hit
ACCCAACAGTAGAGCACCCAGACATACAAAACAAATATTATTAGATCCAAAA2310.10059004115047139No Hit
CGACTTGGGATAAAAGTTTGCACACTGTATGATTCCATTTACATAACATTTT2300.10015458642687626No Hit
TATAACGGGATGCATCAGATGCTGGATCAGACTTTACTGGAGTTAAACAACA2300.10015458642687626No Hit
CGAAGAGGGTGAATGGAAACTTGGAAATACTCGTTTTTATAAACTACAAAAA2300.10015458642687626No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)