FastQCFastQC Report
Sat 31 Dec 2022
SRR5844662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227956
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC9610.4215725841829125No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT9240.4053413816701469RNA PCR Primer, Index 19 (95% over 22bp)
TACTGTGGGTGGTGCTGCCCAGAAAGCTTTGTTGGAAAAAGATTTGAAGCAA3120.1368685184860236No Hit
CAAAGTGGGCACTTCTCTGAATGTGTGATCTCATCACTAAAATGGAATAATG3060.1342364315920616No Hit
CGCTTGGGGGGAGAAGCGTGCCTCAGGCCAGGCTTTTGAGCTGATTCTCAGC2930.12853357665514398No Hit
CGAAGAGGGTGAATGGAAACTTGGAAATACTCGTTTTTATAAACTACAAAAA2930.12853357665514398No Hit
TGAAACCGGGCCAGAAACTACTGGCAGAAGGAAAGAGTACTAGAATTCAGAT2860.1254628086121883No Hit
TAAGCAGGGAGCTGCCCGGAGTGCGCCCGTGTCTTTGCCAGCCCTCTGCGGC2780.12195335942023898No Hit
TCACGAGGGATAAGTAGACATTCTGATTTCTTTTAAAATAAACTAGCAGCCT2780.12195335942023898No Hit
AAGCCAGGGATATTCACAAATTTGCCAAAGCCCAGTTAACTTCAATTCTTCG2740.12019863482426432No Hit
TTATGGGGGAAACTGCGTCTCACAAAAAAGAAAAAAATTGAATAGCCCTATA2720.11932127252627701No Hit
CAGCACGGGTGTTTTGTCTTCCCACAGAGCTCAACAAAGGAGCCGGTTGCCC2720.11932127252627701No Hit
GTTCACGGGACTGTCGGCCCTGCTGCGAGCCCACACGCCCCTCCACATGGCT2680.11756654793030234No Hit
CAATTCGGGGCACGATCTCGGCCCACCGTAACCTCCACCTCCTGGGTTCAAA2680.11756654793030234No Hit
TAGCGCGGGACAGTGATTGAGGAGCACCTGGGGAAGTTTGGCGTCATTTGCT2640.11581182333432768No Hit
TCCGACGGGTGCCCTTTGATGCTTGATTCCAGTGATTGATTCCCTTTGTTGC2630.11537314218533401No Hit
GAATATGGGACAGATACGTGATATTTTGGTATAACTAGAATCTTGATTTCTT2630.11537314218533401No Hit
CATAGAGGGAAATAGAAAGAAGAAAGCCTGTAATCTGTTTCAGAATGCTATT2620.11493446103634035No Hit
TGAATGGGGATTGATTTTCTGTAACTGATAAATCATCATGAAAGTAAGTGTT2610.11449577988734667No Hit
AAAAGCGGGGCTTCTTTTTTAATTTGCTCTTACAATGAAAATACTAATAAAA2570.11274105529137202No Hit
CAAGCAGGGGGAGACATGTCTTGTAAACAGTTGAATGTATGTAAGTTTTCTG2560.11230237414237836No Hit
TATCAAGGGTATGCCAAATACAGTTTGTCATGAGTAATCCATTTAGGGTACA2560.11230237414237836No Hit
TGTACGGGAAGCCACACCCACTCAGACACCCCTCACCTCTCATCTACTTAGA2540.11142501184439103No Hit
GTAAATGGGGAGCTCATTTCTACCAATATATTACTATATTAACTGGAACATT2520.1105476495464037No Hit
TGGCTAAGGGGCAGGAAGGGAACTCCACCACTCAGGGGCAAGCTCGGGAAAA2500.10967028724841636No Hit
ATAATGGGGATATCAGAACGTCCTGTAATCATTTAGTTAGAAACTAACTACT2500.10967028724841636No Hit
ACAACGGGAGTTCAATTGGCTACCGGAAAAAACCAGGCTGGGCTGGGCGCCG2480.10879292495042904No Hit
AAGATTGGGCAAGTGCCTCAAGCATTACTGCTCACCACTCAGCTACCGCCAG2470.10835424380143535No Hit
TATAACGGGATGCATCAGATGCTGGATCAGACTTTACTGGAGTTAAACAACA2450.10747688150344803No Hit
GCACTAGGGCATATTCATAGTAGGAAATTTAGAAAATAGAAAATGATAAAAA2450.10747688150344803No Hit
GTGACTGGGATCTTCTTATGTATTAAAGTGCAATTTCTTTACCACTACTGTC2430.1065995192054607No Hit
GCTATGGGCCTCAGATTTATTTTGTCTTGCCCCATTGCCTCTCCTTCCTTGA2420.10616083805646703No Hit
TTGTATGGGCTTTGTTTTATATAATGCATTCTGCATTTTTAAGAAGTGTTCA2420.10616083805646703No Hit
AGCCCGGGTCCCAGGGCTCCACCACAAATAGTGACTGGATGAAAAACCTCAT2420.10616083805646703No Hit
TTATTTGGGGGGCGCGCGGGTGCGGGGACTACCCGCCAGGCTCCGGCTCGGG2410.10572215690747336No Hit
ACGAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2400.10528347575847971No Hit
TTGTGGGGGCCTAAAACAAGCTGAACATCAAAAAATTGACTGTAATGGATGA2400.10528347575847971No Hit
CGACTTGGGATAAAAGTTTGCACACTGTATGATTCCATTTACATAACATTTT2380.10440611346049238No Hit
ACCAACGGGATGGAGAAGTGAGTGCATAGATATATGAAATCAACATTTCAAA2360.10352875116250504No Hit
ATCCGGGGAGTTCTTCATACCTGTACAAAGATTACCCAGGATCACTTCATTC2350.10309007001351136No Hit
CGCCCGGGAAATGGTCCTGGAGCAGTCAGATATTCATAAGGAAAAACAATCA2350.10309007001351136No Hit
AACTGTGGGTACCCAGCATACTGTTTGCCAGTTGAATGTTATTCAAGCCAGA2350.10309007001351136No Hit
AGCGCAGGGGTTTCTTACAAAACCAGACATTCTTACCATATGATCTAGCAGT2340.10265138886451772No Hit
GTAACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2340.10265138886451772No Hit
TACGAAGGGAGCCTGGAGCACCTTTCCTTCAAGCCAGCAACACAGAGCACTA2340.10265138886451772No Hit
GGGTTCTGCAAGCTCACATTTTAGCTCCCCCTGCTTTCTTGCCATCTCTTCT2330.10221270771552406No Hit
ATATCTGGGATCTAGCCTAAACAAAATTGCTGACACAAAATCCAGCATCGAC2330.10221270771552406No Hit
TAATCGGGCAGTATGACATTTTAAGGAAATAAGTCTGCATTGATGTGATTGC2330.10221270771552406No Hit
GATTTGGGCAGTAATGAGTGGATGAAAACATTTTAAGTAATCTTCTACTATT2320.10177402656653038No Hit
AGCATAGGACATACTTTTATTGAAAATATCTTTTCAGCTAAAACTCATGGCC2320.10177402656653038No Hit
CAATAAGGGAATGATGGCAACCATTAAAATGATAGAAACCACTTACTAATCA2320.10177402656653038No Hit
TGAGTAGGGGGTTGTGGGCATTGTTTTCTACAACCAAATTCTGGGTTTTTTT2310.10133534541753672No Hit
ACAGGGGGTAGGAAGCCAGGAGATCAGTGAGGGTGAGTTAGTGCTTTGCAAA2310.10133534541753672No Hit
GTACCTGGGTGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAAACGAGAGCAC2310.10133534541753672No Hit
TGTTCTGGGCAGTTGCAAAAGGCCACATATTGTGTGCTTCTATTTATGTGAA2310.10133534541753672No Hit
GGACTTGGGTCAGGGTCTGAGGAGGCTGTGACGCCCTATGACCGCAGAGATC2300.10089666426854305No Hit
CTTTGCGGGGTGAAATAAAAATGCAGCACTCCATTTTTTTAAAAATACTGTG2290.1004579831195494No Hit
CGCTGCGGGATCCTAGAATAGCAAATCTGGTGAAAATACATTTCAATCATGA2280.10001930197055572No Hit
AGCTGCGGGCATGGAGGCCTTTAAACCCTTCCTGGGCATTGGATTAAAAAAT2280.10001930197055572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)