FastQCFastQC Report
Sat 31 Dec 2022
SRR5844661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385462
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT12760.33103133382797784RNA PCR Primer, Index 19 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC12610.3271398996528841No Hit
TGACTCGGGGGAGACACTTCGCCGAGGCACAGCAGCCGGCAGGATGGCGACC4460.1157053094727885No Hit
GAGGGAAAGCTTATAGCACTAAATGCCTACATCAGAATTTCAGAGCACAAAT3970.10299329116748215No Hit
CAAACGGGTGAGAAAGGTAATATTGGTAAATAAAGAAATAGGAAACAATGTA3890.10091785960743213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)