FastQCFastQC Report
Sat 31 Dec 2022
SRR5844660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384884
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGCCGTCT12660.3289302750958731RNA PCR Primer, Index 19 (95% over 22bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACGTGTGAGAATCTCGTATGC12330.320356263185791No Hit
TGACTCGGGGGAGACACTTCGCCGAGGCACAGCAGCCGGCAGGATGGCGACC4300.11172197337379573No Hit
CAAACGGGTGAGAAAGGTAATATTGGTAAATAAAGAAATAGGAAACAATGTA4050.1052265098055518No Hit
AAGCCAGGGCTACCCATCGCTCAAGCCTCTGTCATCATGGGTCATGGACCTG4030.1047068727200923No Hit
TCATAGGGGAAAACAGTGAAATTAGAACCTAAAAATCAGAACTATAATTAAG3970.10314796146371374No Hit
GAGGGAAAGCTTATAGCACTAAATGCCTACATCAGAATTTCAGAGCACAAAT3870.10054977603641618No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)