FastQCFastQC Report
Sat 31 Dec 2022
SRR5844625.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844625.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191026
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC8710.45595887470815494No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT5480.28687194413325934No Hit
GCTTGGGAGGCTGAGGCGGGAGAATCAGCTGAACACAGGAGTTGGAGGTTGC2450.12825479254132946No Hit
GTCTAGGGAACCGCAAATCAAAAAATATACAACATATCCACAAAAAATACAA2450.12825479254132946No Hit
GTGAATGGGTATATTCAGAGTGCTCAAAAATCTCAACCAATAATCCTATATC2430.12720781464303288No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT2390.12511385884643977No Hit
TAAACTGGGGCAAATGCAACAAAAACAAAGATAAATAGATGGAACTTAATTA2390.12511385884643977No Hit
AATGCAGGGGGAAATTCTGGACATGTTTTTGAAATATTAAGTTTAATAATTG2380.12459036989729147No Hit
AAGGAACGGGGGGGCAGCTAGGCGCTGTGGTTTCTGGGTCTCCCGGGGGACC2370.1240668809481432No Hit
GATAGGGGTCTAAGAATATTTCCTGCTGTTTATCAAATGCACCCTTAGCTTT2360.1235433919989949No Hit
TGTACAGGGGTGCCAAAGCAATTCAATAAAGGAGGGATATCTTTTTAACAAA2360.1235433919989949No Hit
GTATTAGGGGGTGATCTGCCCACCTAGGCCTCCCAAAGTGCTGGGATTACAG2350.12301990304984661No Hit
AAGATGGGGCTGCCTTTGCCTTCTCGTAGGCGTGGGTTGCCTGTGTCATGGG2340.12249641410069832No Hit
GTAACTGGGAAGGTATACCAGCTATCCAAGGAAGACAGGAAATGCTGAGCAG2330.12197292515155006No Hit
ACATAGGGGAGGCAGAGAATGTTCCAAGTAAGTTCTGGTTCCCTTTTGCTTA2330.12197292515155006No Hit
GCTTCAGGGAAGCATCAACAGGAGTCTTCTTCCCAACACGAGCGCATCCATG2310.1209259472532535No Hit
GTAGTGGGGATGGACTCAAATATTTTGGGGAAGAAAAAGACTAAAAGTTCTA2310.1209259472532535No Hit
CACAGTGGGCGCACATAAGGACTCACATAAACTTAAGTAAAGGAGTGGAAAA2280.11935548040580862No Hit
TATTAAGGGGCGTCGCGTCGCTTTCAGCCTCTGGTGAAGGGCGGCGCGCTTA2280.11935548040580862No Hit
ATCGTTGGGGGGAACTGTAATGAGATCTCTTGGGCAGAATCCCACAGAAGCA2270.11883199145666035No Hit
CTCCACGGGGTTAAAGCAGTATTTAGAGGGAAACTTAATAGCACTAAATGCC2270.11883199145666035No Hit
CACCTTGGGGTTGGAGAAAATGGATTTGAAGGAGTTCGACTTGGATGCCCTG2240.1172615246092155No Hit
AATGCTGGGCGATCTAAGCACGGGAAGGCTCTGGGATAGATTTTCCTTCTCA2230.11673803566006721No Hit
CAACCAGGGAGTACTGGAAGTCCTTGGATCAAAAGATAGTCAGTGTGACCGG2220.11621454671091892No Hit
GAATAAGGGAACCCACAGGCAGGAGAGACAGACAAGCCAGAATCATGATCTG2210.11569105776177065No Hit
CGATATGGGGATAACCATGACCATCAAGGCAGTATCAACATGCTTTGAAACA2210.11569105776177065No Hit
CAAGTTGGGGTGTGACCAAATTAGCCAGTCTGTTTTCTTCCATGTTTAATTA2200.11516756881262237No Hit
TATTACGGGCCACCTCTACACGGGTTCCGGGTACCGAATCCGGTACTCGGAA2190.1146440798634741No Hit
GCACTGGGATTTTTGTAGCTATTGTAAAAGGAATTGCTTTCTTAATTTCTTG2190.1146440798634741No Hit
AACATGGGGGTCTTCTAAGCAAAGTAATTTTCTTGAACTTAAATAACAAATT2180.1141205909143258No Hit
CTACTAGAGGCACCCAAGAGGAAGGAGACTGTTAGTTTTCTAGGGCTGCCAT2180.1141205909143258No Hit
TTACCGGGGGCTCCAAAATCCAAAACTTTTTGAGTGCCAACATGACAGCACA2170.11359710196517751No Hit
CACAAAGGGCTAGAATTTAGTAGTAAATTCCATAGTGGTGACAACCATAAAA2160.11307361301602922No Hit
GGCGCAGGAGGAGAAAGAAATAAAACAAAAGGGAGAGGCAACAGGAAAAGAG2150.11255012406688095No Hit
GTAAAGGGGCCTCCCTAATTCACGTTATCACCTGTTTGTCTTTTAGTGTACT2130.1115031461685844No Hit
TTAGGGGGTTTGTCTTCCTTCCCAGTAAAGACAAATTCACTGTCTTCCCAGC2130.1115031461685844No Hit
ACGTGCGGGATGCTCCATCTTACATTTTTGATCATTCACTCTGGCAGGTATG2120.1109796572194361No Hit
TAGACCGGGGCAGCAGACAGAGGCAGTGCTGGGAGAGTGTCGGGTGCGTTTC2120.1109796572194361No Hit
TCTGTCTGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTG2120.1109796572194361No Hit
ATCTCGGGGGCAATGTGCAATTTGTATGAAAGTTGCATGAGTGTTTCAGTAC2090.10940919037199125No Hit
AGCTTAGGGAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCA2090.10940919037199125No Hit
AGCATAGGGAAGACCCAAACTCTTACCATCTGCCTACTATGAAGTGGAACTC2090.10940919037199125No Hit
GTTAGGGGGATCTGATCTTTTCGAAATCACTAACATGTCACTTTCACTTTAA2090.10940919037199125No Hit
GCAGTATGGGATGAAAAAAGCTTTAAAAATTAATGAAAATTGATGGACTGAA2080.10888570142284296No Hit
AGTCTGGGGCGATCTTCGACTGGGAATTTGACTGGCCATCATATTCAGCGAT2080.10888570142284296No Hit
AACCAAGGGATCAACATCAATTAGGGAACCAAAGTTGCACTATCTGGGCCCA2070.10836221247369467No Hit
TAAGCTGGGAGGCTAAGCTCACTGCCAAAGAGCTCAGCCAGAGTTCTTGAAT2060.1078387235245464No Hit
AACATTGGGATCATCATGAAGGGTTCAGATTACAAACGTTATAGCTTTCCCA2060.1078387235245464No Hit
AGCTAAGGGACATCCTAAGTGTAGCAAGCCTGTCATTTGTCTTTAGTCTTAT2050.10731523457539811No Hit
TCGATAGGGAGAGTTCAAATGAGGAGATTGGTCATATCTGAAAACACAAATA2050.10731523457539811No Hit
AACCTCGGGAAAGAGATGCAGATGCTTCAAAAGCTGTTGAAATTGTTACTTC2050.10731523457539811No Hit
CTGCGGAGGGAAGACTTTCAAATGGAGAAAGATAAGCTCTCAACATAAACAG2040.10679174562624984No Hit
CACCAGGGATAGCAGTCTTATAGAACAGATTCTATTATTATGCCAATTTTAT2040.10679174562624984No Hit
AGCCTAGAAGACCCCAGAAATGTGACAGAAAAGAGGGTAAAACAAAACAGGG2040.10679174562624984No Hit
GCTACAGGGGGGGTAACCGGGGATCACCATGGCGGCCTCATTGGTGGGGAAG2040.10679174562624984No Hit
CTACCGATGGGGGTGCTAGTAAAATTAGATATCCATGTGAAAAAGAATGAAA2030.10626825667710155No Hit
CAAGTTGGGGGGGACTAAAAGTTAAAATCAAATAAAGATTCATAAACAAGAG2020.10574476772795326No Hit
GCTTTTGGGATGTTTCTTTGTTTACTTCATTTTTTCCTCTAAATATTCAAGA2010.10522127877880497No Hit
TAGCTTGGGGAGAAGGCAATACTTCCAAACTTATTTTACAAAGCCAATATTA2000.1046977898296567No Hit
ACGCAGGGGACCTCAGTAATGGGGGAAAAAAATCTGCCCTAAACAACAATGC2000.1046977898296567No Hit
TTAGTGGGGATTGATCTTGCCCCCAATCCTGCTCCTCCTCTCATGTTTCCTC2000.1046977898296567No Hit
AATAAAGGGACATGAGCTCAGTATAAATATAAAAGTAAATTTTATTGCACTG1980.10365081193136014No Hit
AAAACAGGGTTGGTATAGTATTGATAATTTTTATTATATTTTTTGCAGATTG1980.10365081193136014No Hit
AAAATAGGGGTTGGGTTTTTTTGTTTTTGTTTTTTTTAACTTTTCCAAAACC1980.10365081193136014No Hit
CGCACTGGGTGCTGGTGGGAATGTTCAAATCTTTCCTAAAGATCCTTCAACT1980.10365081193136014No Hit
TTGCTAGGGCAGGGATGTATCTGGAGCATAGATAATCAGAACCCCGCAAACA1970.10312732298221185No Hit
ACGAACGGGTACTTCAAAACCACAGCTGCTGTAAATTCTTAAACACTGGAGA1970.10312732298221185No Hit
TTACGAGGGGTACTTCTGAATAATTCGTGGGTCAAAGGAAAAATGATAAAGG1970.10312732298221185No Hit
ACAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1960.10260383403306356No Hit
TTAGCCGGGGAGGGTGCTACCTTGATGTAGTATGCTCCCCCTTTTCCTAGAG1950.10208034508391527No Hit
GCACTTGGGAGTCTTTCACATTTCATTCCTGGGTTTTGCCCCACCATGGAAA1950.10208034508391527No Hit
CTTAATTAGGGGATCTCATGGTGGGCTAGTCAGCTGGCTCTTTTTCCTATGA1950.10208034508391527No Hit
TTAAGTGGGACAGTGTTGTACAACCACCACATGTATCTAGTTCCAAAACATT1950.10208034508391527No Hit
ATAGCAGGGTATCAGTGGATACCAAGCTCTGTATCCATTTGTCCCCTGCCCT1940.10155685613476699No Hit
GTATAGGGGAGCACTTCTGTATCCTTTGAAGCATAGTTTAAAAACCATTGAT1940.10155685613476699No Hit
TGTAAGGGCTATGTCTCAAGGAGAGATTACCAAGTTGGCAGTGAGACAGAAG1930.10103336718561871No Hit
GGCAACGGGATCCTTGGTAGGGAAGGAAGACAGCTGACATGGGAAGGCTCCA1930.10103336718561871No Hit
GACCACGGGGGGCTCCAGTTTGACTTTGTGACAGAAGTCCTAGGACAAGTCT1930.10103336718561871No Hit
AATGCTGGGAACTCAGGGAAACGGGAAGAACTGCAGGAATCAGAACTCCTGT1920.10050987823647044No Hit
CTTCAAGGGCATTGCTTGGAACCCTGAGAATACCATGGCTCATCCTGGTATC1920.10050987823647044No Hit
TCTCATGGGTGTAACCACTGGGGAGCACTGCCAAAAATACAGCTTTCTGGTT1920.10050987823647044No Hit
ATCTAAGGGATCAGTTGAATGTACTTAAAAAAGTGCAAGATGCTAGAAAGAA1920.10050987823647044No Hit
AGTGAAGGGTGCCTGGGTACAGAAAGCCTTAATCCATAAAATCTATATTAAA1920.10050987823647044No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)