FastQCFastQC Report
Sat 31 Dec 2022
SRR5844624.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844624.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191574
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC8350.4358629041519204No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT4990.26047375948719553No Hit
AAGATGGGGCTGCCTTTGCCTTCTCGTAGGCGTGGGTTGCCTGTGTCATGGG2570.1341518160084354No Hit
TCTGTCTGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTG2570.1341518160084354No Hit
GCCTTGGGGATCTTCCAATAGGAAGTCAGCAGTTTCTGGTACAATTAACCGG2490.1299758839926086No Hit
TAAACTGGGGCAAATGCAACAAAAACAAAGATAAATAGATGGAACTTAATTA2480.12945389249063027No Hit
GCTTCAGGGAAGCATCAACAGGAGTCTTCTTCCCAACACGAGCGCATCCATG2450.1278879179846952No Hit
GTAACTGGGAAGGTATACCAGCTATCCAAGGAAGACAGGAAATGCTGAGCAG2410.12579995197678182No Hit
AAAACAGGGTTGGTATAGTATTGATAATTTTTATTATATTTTTTGCAGATTG2330.12162401996095504No Hit
CACAGTGGGCGCACATAAGGACTCACATAAACTTAAGTAAAGGAGTGGAAAA2320.12110202845897669No Hit
CTTAATTAGGGGATCTCATGGTGGGCTAGTCAGCTGGCTCTTTTTCCTATGA2300.12005804545502No Hit
TTAGGGGGTTTGTCTTCCTTCCCAGTAAAGACAAATTCACTGTCTTCCCAGC2290.11953605395304163No Hit
CATCCTGGGAGTGCACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAA2280.1190140624510633No Hit
GAATAAGGGAACCCACAGGCAGGAGAGACAGACAAGCCAGAATCATGATCTG2270.11849207094908494No Hit
CAAGTTGGGGGGGACTAAAAGTTAAAATCAAATAAAGATTCATAAACAAGAG2270.11849207094908494No Hit
AGCCTAGAAGACCCCAGAAATGTGACAGAAAAGAGGGTAAAACAAAACAGGG2250.11744808794512825No Hit
TCGATAGGGAGAGTTCAAATGAGGAGATTGGTCATATCTGAAAACACAAATA2240.11692609644314991No Hit
GTCTAGGGAACCGCAAATCAAAAAATATACAACATATCCACAAAAAATACAA2240.11692609644314991No Hit
ACGCAGGGGACCTCAGTAATGGGGGAAAAAAATCTGCCCTAAACAACAATGC2240.11692609644314991No Hit
ATCGTTGGGGGGAACTGTAATGAGATCTCTTGGGCAGAATCCCACAGAAGCA2230.11640410494117157No Hit
CTCCACGGGGTTAAAGCAGTATTTAGAGGGAAACTTAATAGCACTAAATGCC2230.11640410494117157No Hit
ATCTCGGGGGCAATGTGCAATTTGTATGAAAGTTGCATGAGTGTTTCAGTAC2220.1158821134391932No Hit
GTAGTGGGGATGGACTCAAATATTTTGGGGAAGAAAAAGACTAAAAGTTCTA2220.1158821134391932No Hit
GTAAAGGGGCCTCCCTAATTCACGTTATCACCTGTTTGTCTTTTAGTGTACT2210.11536012193721487No Hit
GCTTGGGAGGCTGAGGCGGGAGAATCAGCTGAACACAGGAGTTGGAGGTTGC2210.11536012193721487No Hit
CGATATGGGGATAACCATGACCATCAAGGCAGTATCAACATGCTTTGAAACA2210.11536012193721487No Hit
ACATAGGGGAGGCAGAGAATGTTCCAAGTAAGTTCTGGTTCCCTTTTGCTTA2200.11483813043523651No Hit
CACCAGGGATAGCAGTCTTATAGAACAGATTCTATTATTATGCCAATTTTAT2190.11431613893325818No Hit
GTAGCAGGGAAGACACACTATCTGCATTTTCCTACTTTAAAATAATAAACAT2190.11431613893325818No Hit
TTACCGGGGGCTCCAAAATCCAAAACTTTTTGAGTGCCAACATGACAGCACA2190.11431613893325818No Hit
ACGAACGGGTACTTCAAAACCACAGCTGCTGTAAATTCTTAAACACTGGAGA2180.11379414743127982No Hit
GCACTGGGATTTTTGTAGCTATTGTAAAAGGAATTGCTTTCTTAATTTCTTG2180.11379414743127982No Hit
TATTACGGGCCACCTCTACACGGGTTCCGGGTACCGAATCCGGTACTCGGAA2170.11327215592930147No Hit
CAACCAGGGAGTACTGGAAGTCCTTGGATCAAAAGATAGTCAGTGTGACCGG2170.11327215592930147No Hit
GTGAATGGGTATATTCAGAGTGCTCAAAAATCTCAACCAATAATCCTATATC2170.11327215592930147No Hit
AACATTGGGATCATCATGAAGGGTTCAGATTACAAACGTTATAGCTTTCCCA2160.11275016442732312No Hit
AACCTCGGGAAAGAGATGCAGATGCTTCAAAAGCTGTTGAAATTGTTACTTC2120.11066219841940972No Hit
AATGCTGGGAACTCAGGGAAACGGGAAGAACTGCAGGAATCAGAACTCCTGT2110.11014020691743139No Hit
AAAAAAGGGTAACTGGGTGGTTTGAGGGTGACTGAAGTCGTGCTTTTCCTGT2110.11014020691743139No Hit
AGTGAAGGGTGCCTGGGTACAGAAAGCCTTAATCCATAAAATCTATATTAAA2100.10961821541545302No Hit
GCTCCCGGGGATTGAGGAATTGAGGAACAACAAGGATATGACATGTAAAAAA2090.10909622391347469No Hit
AACATGGGGGTCTTCTAAGCAAAGTAATTTTCTTGAACTTAAATAACAAATT2090.10909622391347469No Hit
TGTACAGGGGTGCCAAAGCAATTCAATAAAGGAGGGATATCTTTTTAACAAA2080.10857423241149634No Hit
GTAGTGGGGCAGTCAAGAAAAATGACAACAGATGTTGCTTCATCAAGACTGC2080.10857423241149634No Hit
ATAGCAGGGTATCAGTGGATACCAAGCTCTGTATCCATTTGTCCCCTGCCCT2070.10805224090951801No Hit
AACCTAGGGATTAGCTGACTTGGCACTAAATTTTGCTGCACGCAGGCTGAAA2070.10805224090951801No Hit
CGCACTGGGTGCTGGTGGGAATGTTCAAATCTTTCCTAAAGATCCTTCAACT2070.10805224090951801No Hit
AGCTGAGGGGAACCTGTTCCTCAAGTTTAGTCTTATCTCTAGTATTTAAATT2070.10805224090951801No Hit
AAGGAACGGGGGGGCAGCTAGGCGCTGTGGTTTCTGGGTCTCCCGGGGGACC2060.10753024940753965No Hit
GGCGCAGGAGGAGAAAGAAATAAAACAAAAGGGAGAGGCAACAGGAAAAGAG2050.10700825790556129No Hit
AATGCAGGGGGAAATTCTGGACATGTTTTTGAAATATTAAGTTTAATAATTG2050.10700825790556129No Hit
GACCACGGGGGGCTCCAGTTTGACTTTGTGACAGAAGTCCTAGGACAAGTCT2050.10700825790556129No Hit
CACAAAGGGCTAGAATTTAGTAGTAAATTCCATAGTGGTGACAACCATAAAA2050.10700825790556129No Hit
TCGCCTGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTG2040.10648626640358295No Hit
AGCTAAGGGACATCCTAAGTGTAGCAAGCCTGTCATTTGTCTTTAGTCTTAT2040.10648626640358295No Hit
AACCAAGGGATCAACATCAATTAGGGAACCAAAGTTGCACTATCTGGGCCCA2020.10544228339962626No Hit
TGTGCAGGGAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCACA2020.10544228339962626No Hit
CTACTAGAGGCACCCAAGAGGAAGGAGACTGTTAGTTTTCTAGGGCTGCCAT2020.10544228339962626No Hit
TATGCCGGGAAGGTTACCCAGGAATTGAACTCAGCTCTGCACCAAGCGGACC2020.10544228339962626No Hit
GCTACAGGGGGGGTAACCGGGGATCACCATGGCGGCCTCATTGGTGGGGAAG2020.10544228339962626No Hit
CTCACTGAGGGACGAGGCAATATTGTAGTCCACAGGGAATGGATGCTTCACT2010.1049202918976479No Hit
GTAACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2010.1049202918976479No Hit
CTAGCTGGGGAGCTGAAGACAGGAAGCAGTCAAAAACTCTCCATGAGTGAAG2010.1049202918976479No Hit
CTTCAAGGGCATTGCTTGGAACCCTGAGAATACCATGGCTCATCCTGGTATC2000.10439830039566955No Hit
CAAGTTGGGGTGTGACCAAATTAGCCAGTCTGTTTTCTTCCATGTTTAATTA1990.10387630889369122No Hit
AGCATAGGGAAGACCCAAACTCTTACCATCTGCCTACTATGAAGTGGAACTC1980.10335431739171286No Hit
ATCGATGGGCAACTGTTGCACTGGGGACTAAGTTCAACACATGAACTTTAGG1980.10335431739171286No Hit
TTTATAGGCTTGTGGGTTGAATCCTTCCTCAAACCAGTTCCCTACATGGTAG1970.10283232588973452No Hit
GCTTATGGGCTGCTCTCTCAGCTCTTGTTCAAATAGTGCTGGAAGTTCTAGC1970.10283232588973452No Hit
GTATTAGGGGGTGATCTGCCCACCTAGGCCTCCCAAAGTGCTGGGATTACAG1960.10231033438775616No Hit
ATTGGCGGGAGAGCCAACGGAACATAAGTGGGAGTGATATATGTAATTTGGG1950.10178834288577782No Hit
GCAACAAAAGACACATTTAATAGAAGTAAAACACACAAGACCGCTGCCTGGT1950.10178834288577782No Hit
GTCACGGGGGAATCCGTTTTCCTCAGGCTTTTAATGACTTCTTATGCAATTC1950.10178834288577782No Hit
CATAACGGGATTGGCTCTGGGCATTATTCTTTGGGGACATCGATCCCTTTTA1950.10178834288577782No Hit
ACTTTTGGGATTGCAAGTAGCTGCAGCAAGCTATAGTCAGCAGACTGGACCC1940.10126635138379947No Hit
TCAAGGGGGAAAACGAAAAAGAACAAACAGAAAACACCTGGAAATGGAGATG1940.10126635138379947No Hit
AAAATAGGGGTTGGGTTTTTTTGTTTTTGTTTTTTTTAACTTTTCCAAAACC1940.10126635138379947No Hit
GTTTGGGGAATGATTATTCCTACAAGTGAAACACTAGACTATTTGGAGTGTA1940.10126635138379947No Hit
ACGTGCGGGATGCTCCATCTTACATTTTTGATCATTCACTCTGGCAGGTATG1930.10074435988182112No Hit
GCCAATGGACCCCACGGGTCTTTATATCACTTTTACGCTTCCTGCAGAAAGA1930.10074435988182112No Hit
AGCTTAGGGAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCA1930.10074435988182112No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT1930.10074435988182112No Hit
CAGCAAGGGTGAACAAATGAGGTTCTGCTTAATGCACTTTGTCTTGCCTTTC1930.10074435988182112No Hit
TTAGCCGGGGAGGGTGCTACCTTGATGTAGTATGCTCCCCCTTTTCCTAGAG1920.10022236837984279No Hit
CTGATTGGGATTTCTTTAGAGGAAAAGAATGAATCTATATTTAGCATATACC1920.10022236837984279No Hit
TTTTAAGGGATCTACCTCTGTTTTTAATTTTCTTTCCAAAAGGCAGCTTGAA1920.10022236837984279No Hit
GCACATGGGATGTTCCATTCAGTCTGCAGCTATTCACACACACACACACACA1920.10022236837984279No Hit
TTGACTGGGACAGCATGGTGACTACAATTAATAACAATATACTGTATGCTGG1920.10022236837984279No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)