FastQCFastQC Report
Sat 31 Dec 2022
SRR5844623.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844623.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190151
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC8650.454901630809199No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT5780.3039689509915804No Hit
GTGAATGGGTATATTCAGAGTGCTCAAAAATCTCAACCAATAATCCTATATC2580.13568164248413103No Hit
GTAAAGGGGCCTCCCTAATTCACGTTATCACCTGTTTGTCTTTTAGTGTACT2460.12937086841510168No Hit
CACCAGGGATAGCAGTCTTATAGAACAGATTCTATTATTATGCCAATTTTAT2400.126215481380587No Hit
ACGCAGGGGACCTCAGTAATGGGGGAAAAAAATCTGCCCTAAACAACAATGC2320.12200829866790078No Hit
CATCCTGGGAGTGCACCAAGATGGCCGCCCCCGTGGATCTAGAGCTGAAGAA2300.12095650298972922No Hit
AAGATGGGGCTGCCTTTGCCTTCTCGTAGGCGTGGGTTGCCTGTGTCATGGG2280.11990470731155765No Hit
GCTTCAGGGAAGCATCAACAGGAGTCTTCTTCCCAACACGAGCGCATCCATG2240.11780111595521454No Hit
AAAATAGGGGTTGGGTTTTTTTGTTTTTGTTTTTTTTAACTTTTCCAAAACC2230.11727521811612876No Hit
AGCCTAGAAGACCCCAGAAATGTGACAGAAAAGAGGGTAAAACAAAACAGGG2230.11727521811612876No Hit
GTAACTGGGAAGGTATACCAGCTATCCAAGGAAGACAGGAAATGCTGAGCAG2230.11727521811612876No Hit
GTAGTGGGGATGGACTCAAATATTTTGGGGAAGAAAAAGACTAAAAGTTCTA2220.11674932027704299No Hit
ACGAACGGGTACTTCAAAACCACAGCTGCTGTAAATTCTTAAACACTGGAGA2210.1162234224379572No Hit
GTCTAGGGAACCGCAAATCAAAAAATATACAACATATCCACAAAAAATACAA2210.1162234224379572No Hit
ACATAGGGGAGGCAGAGAATGTTCCAAGTAAGTTCTGGTTCCCTTTTGCTTA2210.1162234224379572No Hit
GAATAAGGGAACCCACAGGCAGGAGAGACAGACAAGCCAGAATCATGATCTG2200.11569752459887142No Hit
TTGCTAGGGCAGGGATGTATCTGGAGCATAGATAATCAGAACCCCGCAAACA2190.11517162675978565No Hit
TGTACAGGGGTGCCAAAGCAATTCAATAAAGGAGGGATATCTTTTTAACAAA2190.11517162675978565No Hit
GCTTGGGAGGCTGAGGCGGGAGAATCAGCTGAACACAGGAGTTGGAGGTTGC2180.11464572892069987No Hit
AAAACAGGGTTGGTATAGTATTGATAATTTTTATTATATTTTTTGCAGATTG2160.11359393324252831No Hit
TATGCCGGGAAGGTTACCCAGGAATTGAACTCAGCTCTGCACCAAGCGGACC2160.11359393324252831No Hit
AAGGAACGGGGGGGCAGCTAGGCGCTGTGGTTTCTGGGTCTCCCGGGGGACC2150.11306803540344253No Hit
CACAAAGGGCTAGAATTTAGTAGTAAATTCCATAGTGGTGACAACCATAAAA2150.11306803540344253No Hit
ATCGTTGGGGGGAACTGTAATGAGATCTCTTGGGCAGAATCCCACAGAAGCA2140.11254213756435674No Hit
GTAATCGGGGGAAGAAAAGTTAAATAGTTGTGGGCAGTTGTTGATAAAAGGA2140.11254213756435674No Hit
CTCCACGGGGTTAAAGCAGTATTTAGAGGGAAACTTAATAGCACTAAATGCC2130.11201623972527097No Hit
TAGACCGGGGCAGCAGACAGAGGCAGTGCTGGGAGAGTGTCGGGTGCGTTTC2130.11201623972527097No Hit
GGCGCAGGAGGAGAAAGAAATAAAACAAAAGGGAGAGGCAACAGGAAAAGAG2120.11149034188618519No Hit
GCCTTGGGGATCTTCCAATAGGAAGTCAGCAGTTTCTGGTACAATTAACCGG2110.1109644440470994No Hit
ATCTCGGGGGCAATGTGCAATTTGTATGAAAGTTGCATGAGTGTTTCAGTAC2110.1109644440470994No Hit
ATATCTGGGGCTCCAGAATTGTAATTGCTTGTAAAATCCTTTTGATAGTTAA2110.1109644440470994No Hit
TTACCGGGGGCTCCAAAATCCAAAACTTTTTGAGTGCCAACATGACAGCACA2110.1109644440470994No Hit
TCGTGCGGCATAATTTTAAGCATACCAGAATCACTCAGTTCTAAATTCTATT2070.1088608526907563No Hit
TTTGCTGGGATGGGATCTGGTTAAAGAAATGTATTATCTATGGGAATAGTTC2070.1088608526907563No Hit
AGCTAAGGGACATCCTAAGTGTAGCAAGCCTGTCATTTGTCTTTAGTCTTAT2060.10833495485167051No Hit
ACTTTTGGGATTGCAAGTAGCTGCAGCAAGCTATAGTCAGCAGACTGGACCC2060.10833495485167051No Hit
CAGCAAGGGTGAACAAATGAGGTTCTGCTTAATGCACTTTGTCTTGCCTTTC2060.10833495485167051No Hit
TTAAGTGGGACAGTGTTGTACAACCACCACATGTATCTAGTTCCAAAACATT2060.10833495485167051No Hit
CTACTAGAGGCACCCAAGAGGAAGGAGACTGTTAGTTTTCTAGGGCTGCCAT2050.10780905701258474No Hit
AATGCAGGGGGAAATTCTGGACATGTTTTTGAAATATTAAGTTTAATAATTG2040.10728315917349895No Hit
CACAGTGGGCGCACATAAGGACTCACATAAACTTAAGTAAAGGAGTGGAAAA2040.10728315917349895No Hit
CAACCAGGGAGTACTGGAAGTCCTTGGATCAAAAGATAGTCAGTGTGACCGG2040.10728315917349895No Hit
AGCTTAGGGAATATCCAAATCAAGCATTCAAAAGACTACATGATCTTATGCA2040.10728315917349895No Hit
GCGAGTGGGTAAGAGAAAAGGGAACTATACTTGAATATATTAAATTATAGAC2040.10728315917349895No Hit
AGCAATGGGGGGGACAGAATTGTGTCTGTAAAAATGTCATTGGTTTTATTAC2030.10675726133441317No Hit
TCGATAGGGAGAGTTCAAATGAGGAGATTGGTCATATCTGAAAACACAAATA2020.1062313634953274No Hit
TCTGTCTGGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTG2020.1062313634953274No Hit
AACATTGGGATCATCATGAAGGGTTCAGATTACAAACGTTATAGCTTTCCCA2020.1062313634953274No Hit
CTCACTGAGGGACGAGGCAATATTGTAGTCCACAGGGAATGGATGCTTCACT2010.10570546565624162No Hit
ACGGTCCGGGCAGAAACCATCACTACAACCAAAATAATGAACATTTCTTACA2010.10570546565624162No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT2010.10570546565624162No Hit
CAAGCAGGGAATTGTTGCAGATTTAATTACCTCATATAAAGCAAAACACATA2010.10570546565624162No Hit
TAAACTGGGGCAAATGCAACAAAAACAAAGATAAATAGATGGAACTTAATTA2010.10570546565624162No Hit
TTACGAGGGGTACTTCTGAATAATTCGTGGGTCAAAGGAAAAATGATAAAGG2010.10570546565624162No Hit
AAGCCTGGGCTTCTTCCGGTTATTGCATTTTCCTGAAGCCTAGTTTTATTAC2000.10517956781715583No Hit
TTTATAGGCTTGTGGGTTGAATCCTTCCTCAAACCAGTTCCCTACATGGTAG1990.10465366997807006No Hit
GGGGTAGGGCAATTTTTGAAGGTAATGATACTGACGATAATTTTCTAAATAA1980.10412777213898428No Hit
CAAACAGGTCTTCGTATGGGTGCGCCCACAGGGGGATGTGTCCGAATGGGCA1980.10412777213898428No Hit
TTTTGTGGATAACAGAATGATTTATATTCCTTTGGGTATATATTCAGTAATG1980.10412777213898428No Hit
TAAGCTGGGAGGCTAAGCTCACTGCCAAAGAGCTCAGCCAGAGTTCTTGAAT1980.10412777213898428No Hit
GTACCAGGGAAGATGAACAGGTGATTATAACCTGCAGTTTTGCTCCTGGGTC1980.10412777213898428No Hit
CACCTTGGGGTTGGAGAAAATGGATTTGAAGGAGTTCGACTTGGATGCCCTG1960.10307597646081272No Hit
ATTACTGGGTTTATTATGAAATGCAAACATTATACATTTTAATCAGTGACTT1960.10307597646081272No Hit
GTAGCAGGGAAGACACACTATCTGCATTTTCCTACTTTAAAATAATAAACAT1950.10255007862172694No Hit
TCTATTGGGGAACTGGAAGGTGCTTTAGGTCTGGTTCAGGGTCGGGCATTCT1950.10255007862172694No Hit
TAACATGGGATGGTTTGGTTTGGTTTGGTTTGGTTTTTTAAATCAAGAAAGT1950.10255007862172694No Hit
GTTAGGGGGATCTGATCTTTTCGAAATCACTAACATGTCACTTTCACTTTAA1950.10255007862172694No Hit
CCATGTGGGACATTTTGAGATTTAACCACATCTGTGCATGAAAACTTAGTTC1950.10255007862172694No Hit
ATCTAAGGGATCAGTTGAATGTACTTAAAAAAGTGCAAGATGCTAGAAAGAA1950.10255007862172694No Hit
GCAGTATGGGATGAAAAAAGCTTTAAAAATTAATGAAAATTGATGGACTGAA1940.10202418078264118No Hit
CGATATGGGGATAACCATGACCATCAAGGCAGTATCAACATGCTTTGAAACA1940.10202418078264118No Hit
CAAGTTGGGGTGTGACCAAATTAGCCAGTCTGTTTTCTTCCATGTTTAATTA1940.10202418078264118No Hit
TATATAGGGCTTTGGCACTAAGTGCAGTCACACTGATTACACACTTAATTTA1930.10149828294355538No Hit
GTATGTCGGGATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTTC1930.10149828294355538No Hit
ATCGATGGGCAACTGTTGCACTGGGGACTAAGTTCAACACATGAACTTTAGG1930.10149828294355538No Hit
AAAAAAGGGTAACTGGGTGGTTTGAGGGTGACTGAAGTCGTGCTTTTCCTGT1930.10149828294355538No Hit
TATTAAGGGGCGTCGCGTCGCTTTCAGCCTCTGGTGAAGGGCGGCGCGCTTA1930.10149828294355538No Hit
GCACTTGGGAGTCTTTCACATTTCATTCCTGGGTTTTGCCCCACCATGGAAA1920.1009723851044696No Hit
TTTTAAGGGATCTACCTCTGTTTTTAATTTTCTTTCCAAAAGGCAGCTTGAA1920.1009723851044696No Hit
TCAACTGGGGGGGGAAGCGAAGTAGGCAGGGGCGAGGCGGCTGGGGACCGCG1920.1009723851044696No Hit
TTCAGTGGCACATAGTAATTTAATGAATTACTATGTAATTCTAATGAATTAA1910.10044648726538384No Hit
CGCACTGGGTGCTGGTGGGAATGTTCAAATCTTTCCTAAAGATCCTTCAACT1910.10044648726538384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)