FastQCFastQC Report
Sat 31 Dec 2022
SRR5844621.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844621.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255789
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT10050.3929019621641275No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC9850.3850830176434483No Hit
AATTATGGGGAGGCAGCTGCCCTGAAAGCATACCAAGAGGATTTGAAAAGAC2740.1071195399333044No Hit
AAGCCGGGAATTCAGATTTCAATTGGGATCTTACAAACAAACTCATTGTTTT2630.10281912044693085No Hit
GTCACTCGGGAGCCTTTACAAGCATTGATGCTTCCAACTCTTCCACAAATTC2630.10281912044693085No Hit
CCACCTGGGAGACTTACGCTTTGCTTCACGCTGTTTTTCAGAGAACCAGGCT2620.10242817322089692No Hit
CCTGGTGCATTCCCCTTGAATTGGCGAGGAGTGGAAGTGTTATGCTAAAAAA2580.10086438431676109No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)