FastQCFastQC Report
Sat 31 Dec 2022
SRR5844604.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844604.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173874
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC10020.5762793747196245No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT9950.572253470904218No Hit
GTCAGTGGGCTATGATTGTACTATTGCACTCCAACCTGGGTGATAGAGACTG2250.12940405120949655No Hit
CTTACCGGGGATTTCTGTGTTCAATCCCTGCCTCTCCATGTAGAGTTACTAG2190.12595327651057664No Hit
TCCGCAGGGTAACTTTGATGTGATAGTGTTCACCTCTGGTCCCAGCTACTAG2030.11675121064679021No Hit
CTAACAGGGAGGCATGAAATTCTGGAGCTTATTATTGAAAAACTACTCAAGC2030.11675121064679021No Hit
CCATTAGGGGTAGGGACATTAGATATCACACATTCTACCTGTTCCCCTTTAT2020.11617608153030355No Hit
GTAGGGGAACTCTCAAAACCAGTTTAAGCTCCATTCCCAGTCAAAGCCCTAC2020.11617608153030355No Hit
GCAGTGGGGTGCAGGGGAAGACCAAAGTTTGCAACTCGAACTGCTTTCGTCC2010.1156009524138169No Hit
GCCATAGGGGAGGCAAGGATTTCAGGAGCGAGGATTTCAGGACTGAGGCAGC1930.11099991948192368No Hit
GCGCAGGGCTGCTCGACGTGAGGGTGAAATGATTGACTTGTGACCTGCCAGG1920.11042479036543704No Hit
GGGCTGGGACTGCATCAGGATATGGTGGTACCAGGAGACGATAAAGTAGAAA1870.10754914478300379No Hit
ACTAGTGGGACAGAGGTCTTGTTTACTTCTTTTCTACTAATTTGCTATGGAA1850.10639888655003048No Hit
TGCATCGGGTGTTTAGCCTTGTGGCCAAATTTGCAAGTTCTATGACAAACCT1850.10639888655003048No Hit
ATGTCTGGGGGGCAATAATGTTGTAGAACAAGTATCTGGTGAGTACTGATAC1820.10467349920057054No Hit
TGAACAGGGGGGGGAAACTTCCTCTTTGTGTGTTACATATTTTTCTTCACCA1810.10409837008408387No Hit
TAAGCTGGGGATCTAAAACTGGGGTACTCAGAGAGGGGGAACGGATCCTGTG1810.10409837008408387No Hit
GGTCACGGGTGAGGCTGTTTTTGACGTTCTCTGCCAAGGATTTAGAGCTTTC1800.10352324096759723No Hit
TAAACTGGGATTACTGAGTATTTACTATTTGTTGGGCATCAAGTTAGGAAGT1790.10294811185111057No Hit
TCTGTTGGGATTCAATAACAGGAAAACAAATAACCCCTATTTAAACATGGGC1790.10294811185111057No Hit
GCGCATGGGATGGCGGGGTTGGGGGGCCGCTGGAGGCCACCTGCCAAGGCGT1780.10237298273462392No Hit
GGTTACGGGTGGCTACACAAGTAGTTTATCATTTTTCTTTATGATGTTCTTT1760.10122272450165062No Hit
CATCGTGGGAGGAATGCAGAATGTCACTGACATTCTGGAAATACTTTGGCAG1760.10122272450165062No Hit
CGTCGAGGGCTGGTGAGAAACCCTATAAATGTAAGGAATGTGGCAAGACCTT1760.10122272450165062No Hit
AGCCAGGGAGATGACATGATCTGATACTTAGAAAAACCAAAAATGCTCCACC1750.10064759538516398No Hit
GACCAGGGAGATCATGAAGTCATAGGCTTCCAAGGCATAGGAAGAGATCTTG1750.10064759538516398No Hit
GCCCACCTTGGCCTCCCAAAGTTCTGGGATTAAAGGTACGAGCCACCGCAAC1740.10007246626867733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)