FastQCFastQC Report
Sat 31 Dec 2022
SRR5844581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159444
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC8330.5224404806703293No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT7370.4622312536062818No Hit
GTTACAGGGGTGGGAGAAAATTTTCGCAATCTATCCATCTGACAAAGGATAA1940.1216728130252628No Hit
GCACATAAATAAATAAATGACAAAAGAAAGAAAAAAAGGCCAAAAAGTCTAC1810.11351948019367301No Hit
AAATGCTGGGACCACTTAATTACTCAACTCTCCATGTATGTTGATACAAGAA1700.1066205062592509No Hit
GCTATTGGGTTTGCAGAACATAACATTCTTTTATCTACCACCTGCTTCCTAA1630.1022302501191641No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)