FastQCFastQC Report
Sat 31 Dec 2022
SRR5844571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207497
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC12440.5995267401456407No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT10520.5069952818594968No Hit
TGCAGTGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2910.14024299146493682No Hit
TTATCGGGGGGCTACATAAAAACCATTCCAAAACTCAGAGACTTAAAACTTA2910.14024299146493682No Hit
TGTCTTGGGACAAGCTGGTCATTGCCTGCAAGAAAGTTATTGAATTAAGCAG2880.13879718742921585No Hit
GGCGCTGGGGACGCTCTTCCGTTCTGTTGTAGGGATATGATTAACATGTTAG2870.13831525275064216No Hit
GCCCGCGGGACACGTCCGGAGGCCTAGCCGTCGCGTACCTAGGATGCCGCGT2850.13735138339349484No Hit
CGTTCTGGGTCCGTTAATATAGGAAATCTGAATCAATGGGTTTGAATAAAAA2840.13686944871492118No Hit
TCGCTAGGGGTAGAAGACATTGAAGGATGCATTAATTTTCAGGAACTATTTT2830.1363875140363475No Hit
CCAACTACTAGGTCTTGATTGTTCCAATTAATGATATATTTGATCTTTTGGA2800.13494171000062652No Hit
CTACCGGGGGGAGAAGCGAAGTACTTCCATCTCAATGATGATCTCCATGTTC2800.13494171000062652No Hit
GTTTCTGGGCAGCCACACCCTGCCCATTGCTGCATCCAGCTCTCTTGATGCT2790.13445977532205286No Hit
TTGACAGGGGTCCGGGCTATTGGTCTGATTCAGATGGGCCATTTTAGGATCA2780.13397784064347917No Hit
GTCAAAGGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGAT2780.13397784064347917No Hit
ACAACAGGGCCATGTAAATTTTAGTATCAATTTTTCGATTTCTGCAAAAGCA2760.13301397128633186No Hit
GTTATTGGGCTGAGACAGCCTTTGTGGTTTCTCTTGATTTCACCATTCTGGT2730.13156816725061085No Hit
GCATTAGGGATGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAG2720.13108623257203716No Hit
ACAAGTGGTTCGAACAGTTTCCTTCAAGATAAAATAATGAATAATTCTAAGA2700.13012236321488985No Hit
AACGTTGGGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTACTCTGCTA2690.1296404285363162No Hit
AGTATTGGGAAAGACTTCATGACTAAAACACCAAAAGCAATGGCAACAAAAG2680.1291584938577425No Hit
TTCATAGGGCTTTCTCCTGGAGGCTTGCTTAGAGCTTTAGTTAAACCCTATC2630.1267488204648742No Hit
TACCTGGGACTGAAACAGCTGCTTATTTTGCCGTTAAAAATTACATGCAGTT2620.12626688578630052No Hit
AACTTAGGGCGACGTGAAGCTGTTTGAGATACAGCGGGTGTGAGGATGAAGC2610.12578495110772686No Hit
GCTATAGGGATCTAAATGCTTCCCAGCACAGATCCAATGAATGTGAGGCTTC2600.1253030164291532No Hit
AAAGTGGGGAACGAATACTTCTGGAAGCTTAACAAAACTAACCCTGCTGTCC2590.12482108175057953No Hit
GATCATGGGTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGCAAAAA2590.12482108175057953No Hit
GTTATGGGGGAGAAGGGGGGTGGCAACATGGCGTCTTCGGGAGCTGGTGACC2580.12433914707200586No Hit
GCAGTCGGGACAACAACTGAAATAATTATGATTTTTTTCTTGTACTCTGGTG2570.12385721239343218No Hit
GCTGGAGAGGTGTTGGAGCCTGCTAATCTTCTGGCTGAAGAGAAGGACGAGG2570.12385721239343218No Hit
CATCGCCGGGGGGCCGTGACACCCGTGGCATCTTCCGAGTCCATCAGTTTGA2570.12385721239343218No Hit
AGATAGGGGCTCTGTGTTCTTAGGAAATACTTTTTCTATTTTCTATTCTAAT2570.12385721239343218No Hit
GCGCCTGGGCCTCCCAAAGTGCTGGGATTACAAGCGTGAGCCACCGCACCTG2550.12289334303628487No Hit
CACTCTGGGTGCACCACAGATGCCACTCTTAAGAGGTTCTTTTGTAAGATTT2550.12289334303628487No Hit
CAAGAAGGGAAGCCGAGAGGTGCAGGAGAAGATCGAGCAGAAGTATGATGCG2550.12289334303628487No Hit
GCATATGGGAGTTCAAGTGCTAGGAGAGTATGTGGGGGATGGGAAGATAATT2540.1224114083577112No Hit
AACTATGGGGGGATATAACCCCTGTTTAATCATAAAAAATTATAAATGACTA2540.1224114083577112No Hit
ATGTATGGGCTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGT2520.12144753900056388No Hit
CACTGAAGTGAGTCAGGAGCATCTTCATAAACCAAGGCAAGCATATTACACC2510.1209656043219902No Hit
GATGTGTGGGTCTTCCGATCTGTTATAGGGGGGTTATAAAACAAGGAGCTGG2500.12048366964341653No Hit
ATTTACGGGACTCCTAAAGTTATTTACTATTTGGCTTCAAGTATAAAATTTT2480.1195198002862692No Hit
ACACGTGGGAAACTATACATCTTTACCAAGAATTGACTGTGTTCTTCCAGAC2460.11855593092912187No Hit
CTTACTGGGATAGCAGCGGGGTTGAGGTTTTCCTTTCTGGACTCTTCCTTTT2460.11855593092912187No Hit
CAAACCGGGGAAATCAAAGTGCTAAATAAGTATAGAGATATTCCATGTCATA2450.11807399625054821No Hit
ATCAACGGGATACCCACTGGGCTAAGTTTCATATCTAAAGCTTGTCACTAAG2440.11759206157197453No Hit
AATAGGGGGAGGCGTAGGCCATGACCATTTGGGGTACGAGAGCCTAATTTTG2440.11759206157197453No Hit
ATGTAAGGGACAGACGAGATCTCGATCGAAGGCGAGATGGCGGACGTGCTAG2430.11711012689340086No Hit
ACGGCGGGCAAAGGGGCCATTTCAGAGACCCACCCTCAGGCACGTATTCTCT2420.11662819221482719No Hit
TTAGTGGGGTCATTAAATTTTTATATCAAATATCATATTGTTTCAGGGCAAC2420.11662819221482719No Hit
ACTGTGCGGGGTTACTCAAGTCCATGGGTGCTCCCTTTATCTTTATCAAGAA2410.11614625753625354No Hit
TAAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2410.11614625753625354No Hit
ATTTCAGGGCCTGGAAACAGAGGACCGGCATCAGAGAGGATATAACTTATCA2400.11566432285767987No Hit
GTAGTAGGGATGCATATTTACCGTGATGTCTGGACCGTACCTGTGCTCCTTG2390.1151823881791062No Hit
TCAGTTAGGGCAACGATAGACTGCTAACACATACTGTCTACAAAAATCTCAA2390.1151823881791062No Hit
GCAGGCGGGGTTACTATAGCTTTGTAGTGTCATTTAAAGTCAGGTAGCATGA2380.11470045350053253No Hit
GTGCTCGGGTATGAGGAGGCCTCGCTATAGCAAAAATAGTTAAAATGCTGAC2380.11470045350053253No Hit
TGCACAGGGTGAGTCTCCAAGGTCTCCATGTGAAACAAGCAAAAAGAGAATT2370.11421851882195888No Hit
CGTAATGGGGTAACTGGTCATGGCCCAGCTGAGGTTCATTCAGTGATATTTA2370.11421851882195888No Hit
GTAAGCGGGAAGAACTAATATTTATGGAGTACTGATATTGCATTAGACATTG2350.11325464946481154No Hit
GACCGTGGGGATCTAAGCTAATTGAGCTTTGTTTTGAACGTGTAAACTTCCA2340.11277271478623788No Hit
AAAACTGGAGGAAAGCCCTATGTGTATCCTCAACATGGAAAGACCTTCAGTG2330.11229078010766422No Hit
ATTGTTGGGCTGCTACTAGATCTATAAATCTACCTGCATCTATAGGTATCTC2330.11229078010766422No Hit
CCTTAAGGGCCTGTTAAAAAGCTGAAACCATCTACTTCTTAACCCAAGTGAT2320.11180884542909054No Hit
ATTTCGGGGATCAGCTTTGTACATCATCATTTTTCTGAATGACCAATCCCAC2310.11132691075051687No Hit
TCATCTGGGCATCTTAGAGAAACTCTCCCACCTAGTAGTAAAACTTAAATGC2310.11132691075051687No Hit
TCTGTTGGGGGGCCAAATCTCAGCAGGCTTTGTGTCCTTCCTTATTTCATAT2310.11132691075051687No Hit
ACCACAGGGGACTTATTTAATTGTACAATCTTGAAGTTAGAAGAATCATGAG2300.11084497607194321No Hit
TGCGCTGGGTAGCACTGCTAAAAGATGGGAAAACACAAGATTATGTTGTTGG2300.11084497607194321No Hit
GACTCCGGGTATAGGCAAATTTCACTGCCTAGTTTTATTGATATCCTGAGTC2300.11084497607194321No Hit
GTCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2290.11036304139336954No Hit
CACACCGGGATAGATATTGTCAGTAACCAAATTACTCACCGAAAGCAATCAA2290.11036304139336954No Hit
CGCTGCGGGCTCCGCCTCCCAGGTTCATGCCATTCTCCCGCCTCAGCCTCCT2290.11036304139336954No Hit
AGCTGTGGGTAACCGTAGTGGATCTACCAATACACCCAATGAGTGGCATACA2280.10988110671479587No Hit
ATAGAGGGGACCACATCCATGCTGAACTAGCAAATGCCAGGTACTTTCTGGA2280.10988110671479587No Hit
TTTCGCGGGGGGTTTTTAAAATGTTCTCATATCCATTACAATTTGGAAAAAC2270.10939917203622221No Hit
GCTGGGGACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCA2270.10939917203622221No Hit
ATTTCAGGGATGTTTCAACGTGCGCAAGCGTTGCGGCGGCGGGCAGAGGACT2270.10939917203622221No Hit
ATGTACGGGAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATACT2260.10891723735764855No Hit
GCAAAAGGGATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCATT2260.10891723735764855No Hit
AGGATGGGACTGTGGAAGGCTGCAGGGACCTCTGCCTAGGAAAGCCAGGTAT2250.10843530267907488No Hit
CGGCTTGGGGAGTGCTAAGAGAATCACTCTCCTTACCTAGTTATCTACAAAT2250.10843530267907488No Hit
CTATATGGGATACGAAAAAGATACTTCTACACACATGTTTACAGCAGCACAA2250.10843530267907488No Hit
GTGCGTGGGACATGAAATGCTGTCCGAATGGTGGCAGAAATGCAGCGACATT2240.1079533680005012No Hit
ATTTCAGGGGAGGTGCCGAGGGGACGTAGCGTCGCCGCGCCAGGTCTCTAGC2240.1079533680005012No Hit
GAACCTGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2240.1079533680005012No Hit
GCTTTAGGGAGGCTACAAGTGTCAAGAAAGATTCTGTTTCGAATATAGCCAC2240.1079533680005012No Hit
TCTCTGGGGAGCAAAAGATGAATGTGGCAACCACTGAAAATTGTGATGATTA2230.10747143332192753No Hit
CGAAGCGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2230.10747143332192753No Hit
GCATAAGGGGAATAATTGAATAATGACTAATACAACTCCCAAAAAATACTAC2220.10698949864335389No Hit
CAACGTGCTGGGATTGCAGGTATGAGCCACCACACCTGGCTGTTCCAGGAGT2220.10698949864335389No Hit
GCCACAGGGGCTCGCGGCTTCCTCCTTCTCCAGATGTGTACCTCCCTACGAG2220.10698949864335389No Hit
GTTGCAGGGACAATTGATCAATAAAATGTATTCCTCCCGTTTAGCTGAAACT2210.10650756396478021No Hit
GTTTACGGGCACTTAGGATTGGTATGTCTTCTTGATTAGTTGAACATTAATC2210.10650756396478021No Hit
CGCGCGGGGGGGTGTGTGGCATTGTGTTCTATGCCAGAGTGACAAAGTTGAT2210.10650756396478021No Hit
CTATACGGGGGCGACTCGTGCGGGTAGGCGTCTGCGCTCGGTTTGAGGGCTC2210.10650756396478021No Hit
TTCATTGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2200.10602562928620654No Hit
CACCTGTGTGACTGCCGGGCCTGTGTGCCACACAATGCCCACCCATTATTAC2200.10602562928620654No Hit
ACGTGCGGGATGATACAGGATACACGGAACGTTATATGGGTCACCCTGACCA2200.10602562928620654No Hit
AAATTTGGGGCTTCTGCCTACCAACGACAAGAAGAAATGGGCTCGACCCCCC2200.10602562928620654No Hit
CGATTAGGGACTTCTTGTCCTTATTCTTCATCATTGTTTTCCCAGGAATAGC2190.1055436946076329No Hit
ATAAGCGAGGGCCCAATTTTAACTCTCCATATGCAGAGACATCACACTGTTT2190.1055436946076329No Hit
AGCCAGGGTAAGTTTTGCATTATCAGTAGAGACGGGGTTTCACCATGTTGGC2190.1055436946076329No Hit
TATAAAGGGATAGCAAAAATATCTTCCATGCAGCTCGTACCCCAGCCACACT2190.1055436946076329No Hit
CTTCACGGGATGTTCAGTATAGTGATCTCACTTAGGAGAATTTGCAGGAGAA2190.1055436946076329No Hit
TCCTAGGGGTTTAGGCAATTTCAATATAATGTAGAAGTAGTAGTGTGCCCTG2180.10506175992905922No Hit
AATAAAGGGGAGATGCCAACATGGCAGCGGTTGGGGCTGGTGGTTCCACCGC2180.10506175992905922No Hit
ATTGGCGGGAGATGGCTTATATTCTGCAGCTTCAGGGTCGAACAGAGGAGGC2180.10506175992905922No Hit
TATATAGGGGAGATCCGGGGACTGCGGATAAATGGCCTTAGGCCGCGGGCAG2170.10457982525048555No Hit
TGGTGAGGGAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCC2170.10457982525048555No Hit
AAGATTGGGATTGAAAACTCCAGGCATCGAAAACAAGAGCAGAAGCACCTTC2170.10457982525048555No Hit
AAAGACGGGAGATTAAGTGAATGGATGAAAAAAATTGCCTATTGATCTTTTG2160.10409789057191188No Hit
TCGCTGGGGATTATGGAATTTTTATTGATACCTTCATGTTATCTTTGAAAGT2160.10409789057191188No Hit
ACAATTGGGGACATTTGTGAAAGCAAAGCAAGAATGAATGCTTTCCCGATCT2160.10409789057191188No Hit
AGTTGTGGGCATGTACTCTATGTGTCACTATTTTGCATTTGTTGCCCTTATT2150.10361595589333822No Hit
TGTTCGGGGGAATTGGATGTGCTTCAAGGTCCTCCTCTTACTCTAAAAATCT2150.10361595589333822No Hit
TCAGTAGGGAGCCTGAGTCTGAGGAGTGCAGAGAAAGGATATATCCAAAAGG2150.10361595589333822No Hit
CTTACGGGGGAACTTTGCCAGCTACCAGAACACTTCTACATGCCTATTATAA2140.10313402121476456No Hit
CATCATGGGATCAAAGGGAATGGTAAACTTGTAGAAGACAGTGACCATGCAT2140.10313402121476456No Hit
GTGGCAGGGCTGAACAAGACAATCTCGTTTTTGATAATTTACTTCTTATAAC2140.10313402121476456No Hit
CGTATAGGGGCAAAAACCTTTTATCCTAGGATAGTATATCTAGCAAAAATAT2130.10265208653619089No Hit
ATAAATGGGGATGTTGCAGTGAGCTGAGATCATGCCATTGTACTCCACCCTG2130.10265208653619089No Hit
TATCTGGGGATCTCGAACAGGGGAAGGATTCAGCACAGAAGAGGTTGTTGTA2130.10265208653619089No Hit
GGGTATGGGGCACTGGGTGGGGAAAGGATAGTGTGTCTTTAACTCTTAAATT2120.10217015185761721No Hit
ATACTTGGGACTCAACATTTTATGTCGGATCTGAAAAATACTCATTTTTAAG2120.10217015185761721No Hit
GCATTTGGGTGGCTTTATCACACAATGGTTTTGTGAGAACAAAGCTGGAGGT2120.10217015185761721No Hit
ATGCGGGAGATAATTGATGCAATTTAGGATTCACGCAAGTTTCAGTGTCAAA2120.10217015185761721No Hit
GTGTTTGGGGACAAGACAGAACTTTGTAACAAACAGTACCAATTTATTTTGG2110.10168821717904356No Hit
CTTGCAGGGAAAGCATCCATTATGAGCTGAGGTCAGCCTGGATCTGGAATTG2110.10168821717904356No Hit
TGCAATGGGCCAGGGATTAGGGTGGGAAATCTTGAGGGGAGAGAATTTGTAG2110.10168821717904356No Hit
AAGTACGGGCTCAGATGGAAATGAAAAAGACGTTTTTTGGACAGTAGAGGAA2110.10168821717904356No Hit
TAAAACGGGATGACATTTGCAGATACAAAAGATGGAAATTTTTCTGTAAAAC2100.10120628250046988No Hit
GAATTCGGGTGCCTCAGTCATGCTAGCTGTGTGACTTTGGGTAAGTTACCTA2100.10120628250046988No Hit
GTTCATGGGCTGCTGATATTCCCACTGCCTATAATTGCCAAATTAATAATGA2100.10120628250046988No Hit
GCTGCGGGGCATTAGTAAGACTTAATTATTATTGAAATGAACTGTCTGGTAA2100.10120628250046988No Hit
GGCCTTGGGGAGGACCAAGAAGAAGAGCTGTAAGAAACACAAGGTAAGTTCT2090.10072434782189621No Hit
TTGAGCGGGCTCCTTTAGCTTACTTGCATTTAATTTCATATACCTTATTTAT2080.10024241314332255No Hit
TCAACAGGGAACATCCATGTGACTAGTGTATATGGTATTTGCTCTCCATTAG2080.10024241314332255No Hit
TACTTCGGGGAAAGGACAGATTGTAGTGGTTACCTTTAGAGAAGGAGAAATA2080.10024241314332255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)