FastQCFastQC Report
Sat 31 Dec 2022
SRR5844570.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844570.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207982
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC13290.6389976055620198No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT10050.4832148935965613No Hit
GTCAAAGGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGAT3310.1591483878412555No Hit
TTATCGGGGGGCTACATAAAAACCATTCCAAAACTCAGAGACTTAAAACTTA3030.14568568433806772No Hit
GTTATTGGGCTGAGACAGCCTTTGTGGTTTCTCTTGATTTCACCATTCTGGT3030.14568568433806772No Hit
TTCATAGGGCTTTCTCCTGGAGGCTTGCTTAGAGCTTTAGTTAAACCCTATC3030.14568568433806772No Hit
GCTATAGGGATCTAAATGCTTCCCAGCACAGATCCAATGAATGTGAGGCTTC3010.1447240626592686No Hit
TCATCTGGGCATCTTAGAGAAACTCTCCCACCTAGTAGTAAAACTTAAATGC2980.1432816301410699No Hit
CTACCGGGGGGAGAAGCGAAGTACTTCCATCTCAATGATGATCTCCATGTTC2980.1432816301410699No Hit
AACTATGGGGGGATATAACCCCTGTTTAATCATAAAAAATTATAAATGACTA2960.14232000846227078No Hit
CCAACTACTAGGTCTTGATTGTTCCAATTAATGATATATTTGATCTTTTGGA2910.13991595426527295No Hit
CACTCTGGGTGCACCACAGATGCCACTCTTAAGAGGTTCTTTTGTAAGATTT2890.13895433258647383No Hit
GGCGCTGGGGACGCTCTTCCGTTCTGTTGTAGGGATATGATTAACATGTTAG2890.13895433258647383No Hit
CAAGAAGGGAAGCCGAGAGGTGCAGGAGAAGATCGAGCAGAAGTATGATGCG2850.13703108922887558No Hit
CATCGCCGGGGGGCCGTGACACCCGTGGCATCTTCCGAGTCCATCAGTTTGA2840.136550278389476No Hit
AGATAGGGGCTCTGTGTTCTTAGGAAATACTTTTTCTATTTTCTATTCTAAT2800.13462703503187776No Hit
TGGTGAGGGAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCC2780.13366541335307863No Hit
CTTACTGGGATAGCAGCGGGGTTGAGGTTTTCCTTTCTGGACTCTTCCTTTT2780.13366541335307863No Hit
CAACGTGCTGGGATTGCAGGTATGAGCCACCACACCTGGCTGTTCCAGGAGT2750.13222298083487993No Hit
TGTCTTGGGACAAGCTGGTCATTGCCTGCAAGAAAGTTATTGAATTAAGCAG2750.13222298083487993No Hit
CGTTCTGGGTCCGTTAATATAGGAAATCTGAATCAATGGGTTTGAATAAAAA2720.13078054831668126No Hit
CACTGAAGTGAGTCAGGAGCATCTTCATAAACCAAGGCAAGCATATTACACC2720.13078054831668126No Hit
TCGCTAGGGGTAGAAGACATTGAAGGATGCATTAATTTTCAGGAACTATTTT2710.13029973747728169No Hit
ACAAGTGGTTCGAACAGTTTCCTTCAAGATAAAATAATGAATAATTCTAAGA2710.13029973747728169No Hit
AACGTTGGGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTACTCTGCTA2650.1274148724408843No Hit
GCTGGAGAGGTGTTGGAGCCTGCTAATCTTCTGGCTGAAGAGAAGGACGAGG2620.12597243992268561No Hit
TGCAGTGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2610.12549162908328607No Hit
GCTGGGGACCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCA2610.12549162908328607No Hit
ATTTCAGGGGAGGTGCCGAGGGGACGTAGCGTCGCCGCGCCAGGTCTCTAGC2610.12549162908328607No Hit
GTAAGCGGGAAGAACTAATATTTATGGAGTACTGATATTGCATTAGACATTG2610.12549162908328607No Hit
GCATTAGGGATGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAG2600.1250108182438865No Hit
ACAACAGGGCCATGTAAATTTTAGTATCAATTTTTCGATTTCTGCAAAAGCA2580.12404919656508735No Hit
GCCCGCGGGACACGTCCGGAGGCCTAGCCGTCGCGTACCTAGGATGCCGCGT2560.12308757488628823No Hit
GCAGTCGGGACAACAACTGAAATAATTATGATTTTTTTCTTGTACTCTGGTG2560.12308757488628823No Hit
TCAGTTAGGGCAACGATAGACTGCTAACACATACTGTCTACAAAAATCTCAA2560.12308757488628823No Hit
ATTTCGGGGATCAGCTTTGTACATCATCATTTTTCTGAATGACCAATCCCAC2550.12260676404688867No Hit
ACACGTGGGAAACTATACATCTTTACCAAGAATTGACTGTGTTCTTCCAGAC2550.12260676404688867No Hit
TCGCTGGGGATTATGGAATTTTTATTGATACCTTCATGTTATCTTTGAAAGT2550.12260676404688867No Hit
GCGCCTGGGCCTCCCAAAGTGCTGGGATTACAAGCGTGAGCCACCGCACCTG2550.12260676404688867No Hit
ATGTATGGGCTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGT2530.12164514236808954No Hit
CGAAGCGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2530.12164514236808954No Hit
ATTTCAGGGCCTGGAAACAGAGGACCGGCATCAGAGAGGATATAACTTATCA2520.12116433152868998No Hit
AACTTAGGGCGACGTGAAGCTGTTTGAGATACAGCGGGTGTGAGGATGAAGC2510.12068352068929042No Hit
TACCTGGGACTGAAACAGCTGCTTATTTTGCCGTTAAAAATTACATGCAGTT2510.12068352068929042No Hit
CAAACCGGGGAAATCAAAGTGCTAAATAAGTATAGAGATATTCCATGTCATA2500.12020270984989086No Hit
TATCTGGGGATCTCGAACAGGGGAAGGATTCAGCACAGAAGAGGTTGTTGTA2500.12020270984989086No Hit
TGTTCGGGGGAATTGGATGTGCTTCAAGGTCCTCCTCTTACTCTAAAAATCT2490.1197218990104913No Hit
GTTATGGGGGAGAAGGGGGGTGGCAACATGGCGTCTTCGGGAGCTGGTGACC2470.11876027733169216No Hit
ATTTCAGGGATGTTTCAACGTGCGCAAGCGTTGCGGCGGCGGGCAGAGGACT2470.11876027733169216No Hit
TTGACAGGGGTCCGGGCTATTGGTCTGATTCAGATGGGCCATTTTAGGATCA2470.11876027733169216No Hit
CGGCTTGGGGAGTGCTAAGAGAATCACTCTCCTTACCTAGTTATCTACAAAT2460.1182794664922926No Hit
GTTTCTGGGCAGCCACACCCTGCCCATTGCTGCATCCAGCTCTCTTGATGCT2460.1182794664922926No Hit
TCCAAAGGGGAGTAAGTATCCATCTTAAAACTTTTTTCTTAATCTCACTCCA2450.11779865565289303No Hit
AGTATTGGGAAAGACTTCATGACTAAAACACCAAAAGCAATGGCAACAAAAG2440.11731784481349347No Hit
GTAGTAGGGATGCATATTTACCGTGATGTCTGGACCGTACCTGTGCTCCTTG2430.11683703397409391No Hit
GCATATGGGAGTTCAAGTGCTAGGAGAGTATGTGGGGGATGGGAAGATAATT2430.11683703397409391No Hit
GTGTTTGGGTCAAATAAAATGGACACATTTCTAGAAAGACATATCTTCCCAG2430.11683703397409391No Hit
TTAGTGGGGTCATTAAATTTTTATATCAAATATCATATTGTTTCAGGGCAAC2430.11683703397409391No Hit
ACTGTGCGGGGTTACTCAAGTCCATGGGTGCTCCCTTTATCTTTATCAAGAA2410.11587541229529479No Hit
TGCGCTGGGTAGCACTGCTAAAAGATGGGAAAACACAAGATTATGTTGTTGG2410.11587541229529479No Hit
TAGCCGGGGGGAAGAACATGAGAAAGTAACCCTGTAAAATTTTTTAATGAAA2400.11539460145589522No Hit
TAAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2400.11539460145589522No Hit
AAAGTGGGGAACGAATACTTCTGGAAGCTTAACAAAACTAACCCTGCTGTCC2390.11491379061649566No Hit
TGTTCCGGGATCCTCCCCACCTCATAACGCAGCCACTGAGGAAGAGTGGTTT2390.11491379061649566No Hit
TTATGTGGGGTTCAGTCACTCCCTGAAAGGCACTTCTCTCTTGTCCAAACAC2390.11491379061649566No Hit
TTCATTGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTG2380.1144329797770961No Hit
GCATTTGGGTGGCTTTATCACACAATGGTTTTGTGAGAACAAAGCTGGAGGT2370.11395216893769654No Hit
ATCAACGGGATACCCACTGGGCTAAGTTTCATATCTAAAGCTTGTCACTAAG2360.11347135809829698No Hit
ATGTACGGGAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATACT2360.11347135809829698No Hit
GCAGGCGGGGTTACTATAGCTTTGTAGTGTCATTTAAAGTCAGGTAGCATGA2360.11347135809829698No Hit
ATTTACGGGACTCCTAAAGTTATTTACTATTTGGCTTCAAGTATAAAATTTT2360.11347135809829698No Hit
TTGAGCGGGCTCCTTTAGCTTACTTGCATTTAATTTCATATACCTTATTTAT2350.11299054725889741No Hit
GTGCTCGGGTATGAGGAGGCCTCGCTATAGCAAAAATAGTTAAAATGCTGAC2350.11299054725889741No Hit
AATAAAGGGGAGATGCCAACATGGCAGCGGTTGGGGCTGGTGGTTCCACCGC2350.11299054725889741No Hit
CGCTGCGGGCTCCGCCTCCCAGGTTCATGCCATTCTCCCGCCTCAGCCTCCT2350.11299054725889741No Hit
ACGGCGGGCAAAGGGGCCATTTCAGAGACCCACCCTCAGGCACGTATTCTCT2340.11250973641949785No Hit
TAGGCGCGGGCAGATTAAAAGCCTTTCTTACATCTTGAAAATTTTCTAATAT2340.11250973641949785No Hit
CCTTAAGGGCCTGTTAAAAAGCTGAAACCATCTACTTCTTAACCCAAGTGAT2330.11202892558009828No Hit
TTACCACGGGAAATATACTTCTATTAGTTAGGAATTGACAGTAAAACAAAAC2320.11154811474069871No Hit
ACGTGCGGGATGATACAGGATACACGGAACGTTATATGGGTCACCCTGACCA2310.11106730390129915No Hit
AAAACTGGAGGAAAGCCCTATGTGTATCCTCAACATGGAAAGACCTTCAGTG2310.11106730390129915No Hit
TAGTTTGGGGCACCCGGGAGGCGGGGCCAGCGAGGCAAGATGGAGTTAGTGC2310.11106730390129915No Hit
GAACCTGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2310.11106730390129915No Hit
TCAGTAGGGAGCCTGAGTCTGAGGAGTGCAGAGAAAGGATATATCCAAAAGG2310.11106730390129915No Hit
TCTGTTGGGGGGCCAAATCTCAGCAGGCTTTGTGTCCTTCCTTATTTCATAT2310.11106730390129915No Hit
TAAAACGGGATGACATTTGCAGATACAAAAGATGGAAATTTTTCTGTAAAAC2300.11058649306189959No Hit
GTTTACGGGCACTTAGGATTGGTATGTCTTCTTGATTAGTTGAACATTAATC2300.11058649306189959No Hit
CGTAATGGGGTAACTGGTCATGGCCCAGCTGAGGTTCATTCAGTGATATTTA2300.11058649306189959No Hit
AGTTGTGGGCATGTACTCTATGTGTCACTATTTTGCATTTGTTGCCCTTATT2290.11010568222250003No Hit
AGGATGGGACTGTGGAAGGCTGCAGGGACCTCTGCCTAGGAAAGCCAGGTAT2290.11010568222250003No Hit
GCCACAGGGGCTCGCGGCTTCCTCCTTCTCCAGATGTGTACCTCCCTACGAG2290.11010568222250003No Hit
AGCTCTGGGAAGGCAGACGTGTATAACTGTGATGATGCACTGTTCTCATTTG2280.10962487138310047No Hit
TATAATGGGGGTGTGGAAACAGGAAGAGAACTAGAACTGGAAGTAAAGCATT2280.10962487138310047No Hit
GTTGCAGGGACAATTGATCAATAAAATGTATTCCTCCCGTTTAGCTGAAACT2270.1091440605437009No Hit
AAAGACGGGAGATTAAGTGAATGGATGAAAAAAATTGCCTATTGATCTTTTG2270.1091440605437009No Hit
TTCAATGGGCTAAGAAAAAAAGAGAGAAGACCCAGATAAATAAAATTGGAGA2270.1091440605437009No Hit
GCTTTAGGGAGGCTACAAGTGTCAAGAAAGATTCTGTTTCGAATATAGCCAC2270.1091440605437009No Hit
ACATTTGGGACAGCTATGGGTATACCACAGGTAATTTTATTTGTAAAACATT2260.10866324970430134No Hit
TTTGTGGGCTGTTAGAAATGGTGAAATATCATATAATCACTTATAAAGAAAA2260.10866324970430134No Hit
TGCAATGGGCCAGGGATTAGGGTGGGAAATCTTGAGGGGAGAGAATTTGTAG2260.10866324970430134No Hit
GTACGTGGGACACTTGACCAACTGGACTTAATAGACATCTACATGCACCCAT2250.10818243886490178No Hit
CATCTGGGCCTCCCAAAGTGCTGGGATTATAGGCATGCGCCACCATGCCCGG2250.10818243886490178No Hit
ACAATTGGGGACATTTGTGAAAGCAAAGCAAGAATGAATGCTTTCCCGATCT2250.10818243886490178No Hit
TATATGGGGGAAGCTGGAGGTGTTAGACACCAGCCCCCTGCATCCTTCAGTA2250.10818243886490178No Hit
CGATTAGGGACTTCTTGTCCTTATTCTTCATCATTGTTTTCCCAGGAATAGC2240.10770162802550219No Hit
AAGTACGGGCTCAGATGGAAATGAAAAAGACGTTTTTTGGACAGTAGAGGAA2240.10770162802550219No Hit
TCTGCAGGGGGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCGGGGAG2230.10722081718610263No Hit
ATAAGCGAGGGCCCAATTTTAACTCTCCATATGCAGAGACATCACACTGTTT2230.10722081718610263No Hit
CACACCGGGATAGATATTGTCAGTAACCAAATTACTCACCGAAAGCAATCAA2230.10722081718610263No Hit
CGTATAGGGGCAAAAACCTTTTATCCTAGGATAGTATATCTAGCAAAAATAT2220.10674000634670307No Hit
TCACAAGGGACCACTCCCTGCCTCAGCACCAGCGAGGAGTCTTTGGAGGGGG2210.1062591955073035No Hit
GTTCATGGGCTGCTGATATTCCCACTGCCTATAATTGCCAAATTAATAATGA2210.1062591955073035No Hit
CGCGCGGGGGGGTGTGTGGCATTGTGTTCTATGCCAGAGTGACAAAGTTGAT2210.1062591955073035No Hit
GTGGCAGGGCTGAACAAGACAATCTCGTTTTTGATAATTTACTTCTTATAAC2210.1062591955073035No Hit
TATATAGGGGAGATCCGGGGACTGCGGATAAATGGCCTTAGGCCGCGGGCAG2200.10577838466790394No Hit
GATCATGGGTGGTAAGGTGCTGTTCACTTGAACAGGTGCTGTTGCGCAAAAA2200.10577838466790394No Hit
AACCAGGGGATGTGTCAAATGTGTATTTGACAATTGTTGAATTGTCAAACAT2200.10577838466790394No Hit
GACTCCGGGTATAGGCAAATTTCACTGCCTAGTTTTATTGATATCCTGAGTC2200.10577838466790394No Hit
AGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2190.10529757382850438No Hit
CGTACTGGGAAATGATTTAAAGGAAAGAACACAGCCCCTAAATAAAAGAAAA2190.10529757382850438No Hit
GATGTGTGGGTCTTCCGATCTGTTATAGGGGGGTTATAAAACAAGGAGCTGG2190.10529757382850438No Hit
TGTTAAGGGATAATCACGTGAACTACAATTACCACATCATGTAACAAGTCTT2180.10481676298910482No Hit
AAGTAGGGGGGGAGTGTATATGACAGGAAAAGAACTTCGCCAGTGTATGCTA2180.10481676298910482No Hit
GGGTATGGGGCACTGGGTGGGGAAAGGATAGTGTGTCTTTAACTCTTAAATT2180.10481676298910482No Hit
CTATACGGGGGCGACTCGTGCGGGTAGGCGTCTGCGCTCGGTTTGAGGGCTC2170.10433595214970526No Hit
TATAAAGGGATAGCAAAAATATCTTCCATGCAGCTCGTACCCCAGCCACACT2160.1038551413103057No Hit
TTCACTGGGCCATTACAAATGGTCTTGTTATGTAGATCTTTATCAAATGATA2150.10337433047090613No Hit
ACCACAGGGGACTTATTTAATTGTACAATCTTGAAGTTAGAAGAATCATGAG2150.10337433047090613No Hit
CTATATGGGATACGAAAAAGATACTTCTACACACATGTTTACAGCAGCACAA2150.10337433047090613No Hit
AAATTTGGGGCTTCTGCCTACCAACGACAAGAAGAAATGGGCTCGACCCCCC2150.10337433047090613No Hit
CACCTGTGTGACTGCCGGGCCTGTGTGCCACACAATGCCCACCCATTATTAC2140.10289351963150657No Hit
AGCCTGGGGTACCACTTACCACATCTTATCACTGAATCTAACTGACATATGT2130.10241270879210701No Hit
TATCTCGGGCACCTTTTCACGAGAGCTGCTTTCTCATCGCTTTTTGTCCCCC2130.10241270879210701No Hit
CAACTTGGGTGGTAGTCTCTCTTGTGCCTTCACTTGGAGACCCTCCTGAAGG2120.10193189795270745No Hit
TCGCGGGGGATTTCTATGAATTCTAAAAAATATTTTTTTCTATGAAATTACT2110.10145108711330789No Hit
AATAGTGGGAAGGACACTCAACTAGGAAATATGAGAACCAGGTTCTGAATGC2110.10145108711330789No Hit
TACTTCGGGGAAAGGACAGATTGTAGTGGTTACCTTTAGAGAAGGAGAAATA2100.10097027627390831No Hit
CAGCCTGGGCAACAAGAGAGAAACTCCATCTCAAAAAAATAAAATAAAATAA2100.10097027627390831No Hit
CTTACAGGCATACTTTATTGACCCCTGAGAGAGAAGCATGACAAAGGAGCGT2100.10097027627390831No Hit
CTTCACGGGATGTTCAGTATAGTGATCTCACTTAGGAGAATTTGCAGGAGAA2100.10097027627390831No Hit
AATCGCGGGAAGATGAGCAGAGACTCAAACATATCTCCGAGTAGCTCACAAC2100.10097027627390831No Hit
GATTCAGGGTGTATTTTTGCTGTGAAATACCTCATAAGGAAATTATAATTGA2100.10097027627390831No Hit
GTCAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2090.10048946543450875No Hit
GCATAAGGGGAATAATTGAATAATGACTAATACAACTCCCAAAAAATACTAC2090.10048946543450875No Hit
CTTACGGGGGAACTTTGCCAGCTACCAGAACACTTCTACATGCCTATTATAA2090.10048946543450875No Hit
ATATGTGGGAACTGGAGGCAACAGAAAGAAATGAAGTGACTCAGCTTCTGGC2090.10048946543450875No Hit
AATAGGGGGAGGCGTAGGCCATGACCATTTGGGGTACGAGAGCCTAATTTTG2090.10048946543450875No Hit
TGCACAGGGTGAGTCTCCAAGGTCTCCATGTGAAACAAGCAAAAAGAGAATT2080.10000865459510919No Hit
TTGTAAGGGAGATCAGAATAGGAAAGATGAGTGCTACCCAGTTCCAGCTAAT2080.10000865459510919No Hit
TCTCTGGGGAGCAAAAGATGAATGTGGCAACCACTGAAAATTGTGATGATTA2080.10000865459510919No Hit
ATATTAGGGACCGGAGAGAGAATGTATTTGCTGACAACCATTAAAGTCAGAA2080.10000865459510919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)