FastQCFastQC Report
Sat 31 Dec 2022
SRR5844569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204863
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGC12200.5955199328331616No Hit
CTTATACACATCTCCGAGCCCACGAGACACGAGTCTATCTCGTATGCCGTCT10960.5349916773648731No Hit
GGCGCTGGGGACGCTCTTCCGTTCTGTTGTAGGGATATGATTAACATGTTAG3100.1513206386707214No Hit
ACAACAGGGCCATGTAAATTTTAGTATCAATTTTTCGATTTCTGCAAAAGCA2960.14448680337591463No Hit
TTCATAGGGCTTTCTCCTGGAGGCTTGCTTAGAGCTTTAGTTAAACCCTATC2930.14302241009845604No Hit
ACAAGTGGTTCGAACAGTTTCCTTCAAGATAAAATAATGAATAATTCTAAGA2890.14106988572851123No Hit
TGCAGTGGGGTAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTT2860.13960549245105267No Hit
TGTCTTGGGACAAGCTGGTCATTGCCTGCAAGAAAGTTATTGAATTAAGCAG2860.13960549245105267No Hit
AGATAGGGGCTCTGTGTTCTTAGGAAATACTTTTTCTATTTTCTATTCTAAT2820.13765296808110786No Hit
CGTTCTGGGTCCGTTAATATAGGAAATCTGAATCAATGGGTTTGAATAAAAA2780.13570044371116308No Hit
TTATCGGGGGGCTACATAAAAACCATTCCAAAACTCAGAGACTTAAAACTTA2750.13423605043370446No Hit
TCATCTGGGCATCTTAGAGAAACTCTCCCACCTAGTAGTAAAACTTAAATGC2710.13228352606375968No Hit
CTACCGGGGGGAGAAGCGAAGTACTTCCATCTCAATGATGATCTCCATGTTC2710.13228352606375968No Hit
GTCAAAGGGGGCAGTTTGAATCGCGGTGCGACGAAGGAGTAGGTGGTGGGAT2680.13081913278630108No Hit
CCAACTACTAGGTCTTGATTGTTCCAATTAATGATATATTTGATCTTTTGGA2660.1298428706013287No Hit
CAACGTGCTGGGATTGCAGGTATGAGCCACCACACCTGGCTGTTCCAGGAGT2660.1298428706013287No Hit
GCCCGCGGGACACGTCCGGAGGCCTAGCCGTCGCGTACCTAGGATGCCGCGT2650.1293547395088425No Hit
TTGACAGGGGTCCGGGCTATTGGTCTGATTCAGATGGGCCATTTTAGGATCA2650.1293547395088425No Hit
GCAGTCGGGACAACAACTGAAATAATTATGATTTTTTTCTTGTACTCTGGTG2630.1283784773238701No Hit
CGCTGCGGGCTCCGCCTCCCAGGTTCATGCCATTCTCCCGCCTCAGCCTCCT2630.1283784773238701No Hit
GCATTAGGGATGTTCAGTAGGTTGAACTATGAACACTGTCATCAATGTTCAG2620.1278903462313839No Hit
GCGCCTGGGCCTCCCAAAGTGCTGGGATTACAAGCGTGAGCCACCGCACCTG2610.12740221513889768No Hit
CACTGAAGTGAGTCAGGAGCATCTTCATAAACCAAGGCAAGCATATTACACC2610.12740221513889768No Hit
TACCTGGGACTGAAACAGCTGCTTATTTTGCCGTTAAAAATTACATGCAGTT2590.1264259529539253No Hit
ATGTATGGGCTTGAAAGGAGGGTAAAAAGACTAAAATGAGGGGGAACAGAGT2590.1264259529539253No Hit
CGTACTGGGAAATGATTTAAAGGAAAGAACACAGCCCCTAAATAAAAGAAAA2560.12496155967646672No Hit
GCTGGAGAGGTGTTGGAGCCTGCTAATCTTCTGGCTGAAGAGAAGGACGAGG2550.12447342858398051No Hit
TCGCTAGGGGTAGAAGACATTGAAGGATGCATTAATTTTCAGGAACTATTTT2550.12447342858398051No Hit
GACTCCGGGTATAGGCAAATTTCACTGCCTAGTTTTATTGATATCCTGAGTC2540.12398529749149431No Hit
CACACCGGGATAGATATTGTCAGTAACCAAATTACTCACCGAAAGCAATCAA2530.12349716639900811No Hit
AACTATGGGGGGATATAACCCCTGTTTAATCATAAAAAATTATAAATGACTA2530.12349716639900811No Hit
CTTGCAGGGAAAGCATCCATTATGAGCTGAGGTCAGCCTGGATCTGGAATTG2520.12300903530652194No Hit
CACTCTGGGTGCACCACAGATGCCACTCTTAAGAGGTTCTTTTGTAAGATTT2520.12300903530652194No Hit
ATCAACGGGATACCCACTGGGCTAAGTTTCATATCTAAAGCTTGTCACTAAG2500.12203277312154953No Hit
AGTTGTGGGCATGTACTCTATGTGTCACTATTTTGCATTTGTTGCCCTTATT2490.12154464202906333No Hit
GTTATTGGGCTGAGACAGCCTTTGTGGTTTCTCTTGATTTCACCATTCTGGT2490.12154464202906333No Hit
GGCCTTGGGGAGGACCAAGAAGAAGAGCTGTAAGAAACACAAGGTAAGTTCT2490.12154464202906333No Hit
ATGTACGGGAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATACT2480.12105651093657713No Hit
CAAGAAGGGAAGCCGAGAGGTGCAGGAGAAGATCGAGCAGAAGTATGATGCG2470.12056837984409093No Hit
TGTTGCGGGAATGTGCAAACAGAGAAATACTTGAAAGGGAACTCAAATAACC2470.12056837984409093No Hit
AACTTAGGGCGACGTGAAGCTGTTTGAGATACAGCGGGTGTGAGGATGAAGC2450.11959211765911855No Hit
CAAACCGGGGAAATCAAAGTGCTAAATAAGTATAGAGATATTCCATGTCATA2450.11959211765911855No Hit
TATCTGGGGATCTCGAACAGGGGAAGGATTCAGCACAGAAGAGGTTGTTGTA2450.11959211765911855No Hit
AGTATTGGGAAAGACTTCATGACTAAAACACCAAAAGCAATGGCAACAAAAG2440.11910398656663235No Hit
GCTATAGGGATCTAAATGCTTCCCAGCACAGATCCAATGAATGTGAGGCTTC2440.11910398656663235No Hit
GCATTTGGGTGGCTTTATCACACAATGGTTTTGTGAGAACAAAGCTGGAGGT2440.11910398656663235No Hit
TGGTGAGGGAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCC2410.11763959328917374No Hit
GACCGTGGGGATCTAAGCTAATTGAGCTTTGTTTTGAACGTGTAAACTTCCA2400.11715146219668754No Hit
ATTGGCGGGAGATGGCTTATATTCTGCAGCTTCAGGGTCGAACAGAGGAGGC2400.11715146219668754No Hit
GTTATGGGGGAGAAGGGGGGTGGCAACATGGCGTCTTCGGGAGCTGGTGACC2390.11666333110420134No Hit
GTTTCTGGGCAGCCACACCCTGCCCATTGCTGCATCCAGCTCTCTTGATGCT2370.11568706891922896No Hit
CATCATGGGATCAAAGGGAATGGTAAACTTGTAGAAGACAGTGACCATGCAT2370.11568706891922896No Hit
TCAGTAGGGAGCCTGAGTCTGAGGAGTGCAGAGAAAGGATATATCCAAAAGG2370.11568706891922896No Hit
ATGCGGGAGATAATTGATGCAATTTAGGATTCACGCAAGTTTCAGTGTCAAA2370.11568706891922896No Hit
ACTGTGCGGGGTTACTCAAGTCCATGGGTGCTCCCTTTATCTTTATCAAGAA2360.11519893782674276No Hit
CTTACTGGGATAGCAGCGGGGTTGAGGTTTTCCTTTCTGGACTCTTCCTTTT2360.11519893782674276No Hit
CGGCTTGGGGAGTGCTAAGAGAATCACTCTCCTTACCTAGTTATCTACAAAT2350.11471080673425656No Hit
GTTTACGGGCACTTAGGATTGGTATGTCTTCTTGATTAGTTGAACATTAATC2350.11471080673425656No Hit
TATCTCGGGCACCTTTTCACGAGAGCTGCTTTCTCATCGCTTTTTGTCCCCC2340.11422267564177035No Hit
TCATGTGGGAAATTAATTCCTTGATTTTCAAACAAATTGAAGACAAAGGAAA2340.11422267564177035No Hit
AAAACTGGAGGAAAGCCCTATGTGTATCCTCAACATGGAAAGACCTTCAGTG2340.11422267564177035No Hit
GCCACAGGGGCTCGCGGCTTCCTCCTTCTCCAGATGTGTACCTCCCTACGAG2330.11373454454928415No Hit
ATTTACGGGACTCCTAAAGTTATTTACTATTTGGCTTCAAGTATAAAATTTT2330.11373454454928415No Hit
CATCGCCGGGGGGCCGTGACACCCGTGGCATCTTCCGAGTCCATCAGTTTGA2320.11324641345679795No Hit
ATGTAAGGGACAGACGAGATCTCGATCGAAGGCGAGATGGCGGACGTGCTAG2320.11324641345679795No Hit
TTAGTGGGGTCATTAAATTTTTATATCAAATATCATATTGTTTCAGGGCAAC2310.11275828236431175No Hit
TTCACTGGGCCATTACAAATGGTCTTGTTATGTAGATCTTTATCAAATGATA2300.11227015127182557No Hit
ATAAGCGAGGGCCCAATTTTAACTCTCCATATGCAGAGACATCACACTGTTT2300.11227015127182557No Hit
ACCAATGGGACCTCAAAGCACAGACAACAAAGATAAAAATAGACAAATGAGA2300.11227015127182557No Hit
GTTGCAGGGACAATTGATCAATAAAATGTATTCCTCCCGTTTAGCTGAAACT2290.11178202017933937No Hit
TGTTAAGGGATAATCACGTGAACTACAATTACCACATCATGTAACAAGTCTT2280.11129388908685317No Hit
CACCTGTGTGACTGCCGGGCCTGTGTGCCACACAATGCCCACCCATTATTAC2270.11080575799436697No Hit
TAGGCGCGGGCAGATTAAAAGCCTTTCTTACATCTTGAAAATTTTCTAATAT2270.11080575799436697No Hit
GTAGTAGGGATGCATATTTACCGTGATGTCTGGACCGTACCTGTGCTCCTTG2260.11031762690188077No Hit
ATTTCAGGGATGTTTCAACGTGCGCAAGCGTTGCGGCGGCGGGCAGAGGACT2260.11031762690188077No Hit
CGTAATGGGGTAACTGGTCATGGCCCAGCTGAGGTTCATTCAGTGATATTTA2260.11031762690188077No Hit
TAGTTTGGGGCACCCGGGAGGCGGGGCCAGCGAGGCAAGATGGAGTTAGTGC2260.11031762690188077No Hit
CGCGCGGGGGGGTGTGTGGCATTGTGTTCTATGCCAGAGTGACAAAGTTGAT2250.10982949580939456No Hit
ATTTGGGGGAATGTTGCATTCATACGTATAACTAAGCATCTTTGATCTTATA2250.10982949580939456No Hit
TTGGCGGGCAATTGGGATGCTACATTTTCTTGTAAAATATTAAGGTCTTAAT2230.10885323362442219No Hit
TGCACAGGGTGAGTCTCCAAGGTCTCCATGTGAAACAAGCAAAAAGAGAATT2220.10836510253193599No Hit
GCAAAAGGGATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCATT2210.10787697143944978No Hit
GTCCCTGGTTCCTAGATGTGTTCTAAGCAATGCAAATGTCTAATTGTCCCCC2210.10787697143944978No Hit
TAAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2210.10787697143944978No Hit
GTCAGGGAGTCAAAGATAGAGCTAAGGAAAGGCAAAAATGAAACTAAATGAA2210.10787697143944978No Hit
AACGTTGGGGCACTGGTGTTGATTCCTCAGAACGCATCCACCTACTCTGCTA2210.10787697143944978No Hit
GCAGGCGGGGTTACTATAGCTTTGTAGTGTCATTTAAAGTCAGGTAGCATGA2210.10787697143944978No Hit
TTTTCTGGGCAGCTCTCCCCTAGAGACATTACTGGGAGTGGTAAGCATTGTG2200.10738884034696358No Hit
GTATTTGGGGATTGATTGGATTTTATTAGTGAAGTTGGAATCTAGGATTTTG2200.10738884034696358No Hit
TACGACAGGGGTATTGCAAAAGACCAAGGAATTAACCTGGGTTAGGAAAGAT2190.10690070925447738No Hit
ATTTCGGGGATCAGCTTTGTACATCATCATTTTTCTGAATGACCAATCCCAC2180.10641257816199118No Hit
AATAGGGGGAGGCGTAGGCCATGACCATTTGGGGTACGAGAGCCTAATTTTG2180.10641257816199118No Hit
TTTGAAGGGGGGCGGTAGCGGCGGCGGCGGCGGTGGTATCGGCGGCAGCTGT2180.10641257816199118No Hit
TTGAGCGGGCTCCTTTAGCTTACTTGCATTTAATTTCATATACCTTATTTAT2170.10592444706950498No Hit
ATAAATGGGGATGTTGCAGTGAGCTGAGATCATGCCATTGTACTCCACCCTG2170.10592444706950498No Hit
GCTGCGGGGCATTAGTAAGACTTAATTATTATTGAAATGAACTGTCTGGTAA2170.10592444706950498No Hit
ATTTTAGGGCTGTGGATCTGGATGCCTTGATGGCTGATCTTTGCTCTATAGA2170.10592444706950498No Hit
TACTTCGGGGAAAGGACAGATTGTAGTGGTTACCTTTAGAGAAGGAGAAATA2170.10592444706950498No Hit
GCATATGGGAGTTCAAGTGCTAGGAGAGTATGTGGGGGATGGGAAGATAATT2170.10592444706950498No Hit
AGCCAGGGTAAGTTTTGCATTATCAGTAGAGACGGGGTTTCACCATGTTGGC2160.1054363159770188No Hit
ACAAGTTGGGAAAGATAAATATGGTTTTATAAAATTATTGATACACCCACAG2160.1054363159770188No Hit
TATATGGGGGAAGCTGGAGGTGTTAGACACCAGCCCCCTGCATCCTTCAGTA2160.1054363159770188No Hit
TATTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2150.1049481848845326No Hit
TCTAACGGGGGTTCATTTGGCTTCGCATTATGCAAGGTGAAACCGTTTGTTT2150.1049481848845326No Hit
GTACGTGGGACACTTGACCAACTGGACTTAATAGACATCTACATGCACCCAT2150.1049481848845326No Hit
AAATTTGGGGCTTCTGCCTACCAACGACAAGAAGAAATGGGCTCGACCCCCC2150.1049481848845326No Hit
ATTTCAGGGGAGGTGCCGAGGGGACGTAGCGTCGCCGCGCCAGGTCTCTAGC2150.1049481848845326No Hit
TTTCGCGGGGGGTTTTTAAAATGTTCTCATATCCATTACAATTTGGAAAAAC2140.1044600537920464No Hit
TATAATGGGGGTGTGGAAACAGGAAGAGAACTAGAACTGGAAGTAAAGCATT2140.1044600537920464No Hit
GATTCAGGGTGTATTTTTGCTGTGAAATACCTCATAAGGAAATTATAATTGA2140.1044600537920464No Hit
AAAGACGGGAGATTAAGTGAATGGATGAAAAAAATTGCCTATTGATCTTTTG2130.1039719226995602No Hit
TCATCGGGGCCCTGAAGGAGTGGGGCCACTGCTTTGGAATTAAAGAAGAGGA2130.1039719226995602No Hit
GAACCTGGGCCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA2120.103483791607074No Hit
ACCACAGGGGACTTATTTAATTGTACAATCTTGAAGTTAGAAGAATCATGAG2120.103483791607074No Hit
GTGCGTGGGACATGAAATGCTGTCCGAATGGTGGCAGAAATGCAGCGACATT2110.10299566051458779No Hit
CCTTAAGGGCCTGTTAAAAAGCTGAAACCATCTACTTCTTAACCCAAGTGAT2110.10299566051458779No Hit
TTATGTGGGGTTCAGTCACTCCCTGAAAGGCACTTCTCTCTTGTCCAAACAC2110.10299566051458779No Hit
GTTCATGGGCTGCTGATATTCCCACTGCCTATAATTGCCAAATTAATAATGA2100.10250752942210159No Hit
ACGTGCGGGATGATACAGGATACACGGAACGTTATATGGGTCACCCTGACCA2100.10250752942210159No Hit
ACACGCGGGATATTTGCTTACTTTGAATTAACAGCAACTTTCCACAGGTAAA2100.10250752942210159No Hit
ACATTTGGGACAGCTATGGGTATACCACAGGTAATTTTATTTGTAAAACATT2100.10250752942210159No Hit
TCTGTTGGGGGGCCAAATCTCAGCAGGCTTTGTGTCCTTCCTTATTTCATAT2100.10250752942210159No Hit
TCTCTGGGGAGCAAAAGATGAATGTGGCAACCACTGAAAATTGTGATGATTA2090.10201939832961542No Hit
GTGCTCGGGTATGAGGAGGCCTCGCTATAGCAAAAATAGTTAAAATGCTGAC2090.10201939832961542No Hit
GTGTTTGGGTCAAATAAAATGGACACATTTCTAGAAAGACATATCTTCCCAG2090.10201939832961542No Hit
GTCTGAGGGCAAGTTAGGAGTAAACAAGATGTTGCATGGATATAAAGCCTAG2090.10201939832961542No Hit
ACGGCGGGCAAAGGGGCCATTTCAGAGACCCACCCTCAGGCACGTATTCTCT2080.10153126723712921No Hit
TGGTCTGGGGAAGATGGGAGCGACCTCTTCCTGATCGGGAGCTGTTTAAAAG2080.10153126723712921No Hit
CAGAGGCGGGAATCTTGTCATTTATGGCAATGTGGATGGAACTGGAGGACAT2070.10104313614464301No Hit
TGGTCTGGGCATAAGAAGGAACCGCAGCACTGGCCAGTGCAGTACCGTGAGG2070.10104313614464301No Hit
CTATATGGGATACGAAAAAGATACTTCTACACACATGTTTACAGCAGCACAA2070.10104313614464301No Hit
ACGACTGGGAATCAACTGGTAATACAGAATTTACAAAAAATAGTATTATGCA2060.10055500505215681No Hit
CGTTAAGGGCTAAATAAGGAAGTTAATCTTGAGGCAGTAGGAATGGAATTAA2060.10055500505215681No Hit
GATTTGGGGAGAAGGGTCTGTCAGCACACATGGAAAGCCCCCATTTGCACAG2060.10055500505215681No Hit
AAAGTGGGGAACGAATACTTCTGGAAGCTTAACAAAACTAACCCTGCTGTCC2050.10006687395967061No Hit
CGGATGGGGGTGGCATTGAGAAATTATGTCTGGAAAATACAGCCAGTGTAGT2050.10006687395967061No Hit
GCTGGTGGGGCTTTCCAGCCCCGGTACCGGACCCTGCAGCCGCAGAGATGTT2050.10006687395967061No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)