FastQCFastQC Report
Sat 31 Dec 2022
SRR5844565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9903
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT2202.221549025547814No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC2162.1811572250833082No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA410.4140159547611834No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA370.3736241542966778No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT350.3534282540644249No Hit
CATGTGGGGCACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCA320.3231344037160456No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC310.3130364535999192No Hit
ACTGCAGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT290.29284055336766635No Hit
AACACAGGAACCCGAGCCTGGCGACCATGCAGGAGTCGCTGCGGGTGAAGCA290.29284055336766635No Hit
CTTTGCGGGAGGCTCCTAAGTCTTATTACCAAAAATAGCTCAAACAAAAACC260.2625467030192871No Hit
TCAGTAGGGATTTTACTTTCTCCAGTATACCTTGAGAAGCAAACTTTGTAGG260.2625467030192871No Hit
AGAAATGGGGTGCAATTTAAGACAGTTAGCAATTGCTCATTCTAAAATAAGT240.24235080278703422No Hit
TGCCAAGGGTCTTCAAGGGAGAAAGAAAGGGACAAGATAAAATGATAACTAA230.2322528526709078No Hit
TCCACGGGTATAATGTGATTCTTCTCAACGTGATTATTTTTTAAGAATAACA220.22215490255478137No Hit
AGTAAAGGGGGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCCGCAGTT220.22215490255478137No Hit
ACAGTTGGGATGTAGACCCAGAAGAAGCTGTAAGGATTCACACTGATGTCCA220.22215490255478137No Hit
TAACTGGGGATCTTCAATTAGGGATACACATACATATTAATATGCAGAGGAT210.21205695243865494No Hit
CCAGCGTGGGACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCA210.21205695243865494No Hit
GCCATTGGGGCATGAGTTCTGTTAGGAATAAAAGATGAAACTATTGTATCTC210.21205695243865494No Hit
CGAACAGGGATTTTAAGGAATTGAAACCTAGCAATCTTATTGGAGAGACAAG200.20195900232252853No Hit
TCAATTGGGAGTGACTAATAGGGATAATCCAGGTAACTTTGAAGAGATGAGC200.20195900232252853No Hit
GTAGTAGGGAACATTTTAATTAGTCTTTACTGTGGAAGAATGCTATTCTAAA200.20195900232252853No Hit
TTTTACGGAGAGGAGAAAGCAAATAAAAATTCAAAGATATTGAGATAGTACC200.20195900232252853No Hit
AATCCGGGGTATCCTGAAAACCACAGAATGTTTCATTGCCAAATTCAGACAC200.20195900232252853No Hit
CTGAAGGGGCATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAG200.20195900232252853No Hit
CTCGGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC200.20195900232252853No Hit
TTAAAAGGGACTATTTTATCAATCAAGGTGTTATATTTCAGTCAGAAGTGAA200.20195900232252853No Hit
GGCACTGGGGGGATCTCTTCCTTGTAAACTTGTACAGTATGTGGTGACTTTT190.1918610522064021No Hit
AGTACAGGGCCGATCTTGCTGGGGGCTTTTTCGTCTGGGCTGCCAACATGCC180.18176310209027569No Hit
CAGTTGGGGAGAAGATGCATGCCAGAGACTTCACAGTTTCTGCTCTGCATGG180.18176310209027569No Hit
TTTGTCGGGCAAGCTCTGGATCTCTAATTCCATGATTAGTAATGTATGTATG170.17166515197414925No Hit
TCTAACGGGTCTGGTAGAGGGAAGGGAAATGCTGTTGGTGAGAATCGACCTC170.17166515197414925No Hit
GTTAAGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT170.17166515197414925No Hit
TCAGTAGGGGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGAC160.1615672018580228No Hit
TTAGTGGGGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGT160.1615672018580228No Hit
CTCGCTGGGGACTCCTCCCGCTAAGGCGGGAACACGCGGGGCCCAAGATGGC160.1615672018580228No Hit
TATATCTGGGGAAGTAACCCTTGGGCATGACTCCAATCCCAGAATTGCTCAC150.1514692517418964No Hit
TCTTCAGGGATTTCAGGAACTGAATATTTGACTGTTAACCTTTTTCCCATAC150.1514692517418964No Hit
ATCAGGGACTGGGACTGCAGGCGTGTGCCGCCACACCTGGCTAATTTTTATT150.1514692517418964No Hit
TCAGTAGGGCATCTGCTCTAAGGTTTCTGGAAGAGTCATTCCCAAACTTTCA150.1514692517418964No Hit
GAGTCCGGGTAACTTTGCAGCTTCTCCCACCAAGAGGTGATGTTTATTTTCT150.1514692517418964No Hit
TTAATAGGGGTTTAGGTTCTTTTGGACAAACATGATAAACTTCTTCAGATAC150.1514692517418964No Hit
AACCGAGGGTCCTCTCTGTCCTGCCTCAGGCCTAAGAAGCACTCTCCCTTGT150.1514692517418964No Hit
CACTTTGGATGCCAAATTTAGTGTTGAAAGGCTCATTCAAATCAATTGGGAA150.1514692517418964No Hit
AACATTGGGGTCTCTGAAGTATTTCCTCCAGTTTCCCTGCGGGCCCCTATGT150.1514692517418964No Hit
TCTTCCGGGGTCTAGGGTGTTCTGTACTTAGAAAGTAAGAGCTCTAAGTACC140.14137130162576997No Hit
TACACCGGCCTGGGTAACATAGGGAGACCTTGTCTCTGCCATAAATTTAGGA140.14137130162576997No Hit
GCCACAGGGCTGAACGAGGCTGCCATCACCACTTTCTCTGTCCTTGGGCTCT140.14137130162576997No Hit
CGTGACGGGCGATCTTGATCCGGGGCCTGGTGCCACAGCCATGGTTTCCATT140.14137130162576997No Hit
ATACTCGGGCTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGA140.14137130162576997No Hit
TAATTTGGGAGCCTTTTGGAAGTTCAAAGATGAAAGCACCATGTATCAGGAT140.14137130162576997No Hit
AAAAAAGGGTGGCTTCAAGTGGCCTCTGTCCTTCCTTCAGGTTATCTTTGTG130.13127335150964356No Hit
AATCTTGGGGTGTTATTCCTTTTTCTATAAATATATTTTTAGGAAAAAAAGT130.13127335150964356No Hit
CTAATGAGGGGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCT130.13127335150964356No Hit
GCCACCGGTGTTCCAACTGTTTAAAATTGATCAGGGACCATGAAAAGAAACT130.13127335150964356No Hit
ACGTGCGGGCTTGGAGACCACTTTTATTTACTCATGAGAAGTGGAAGCTCCT130.13127335150964356No Hit
AATGCGGGGCTATCGAGTTAAGGAGATTGGCAGCACCATGTCTGGCAGAAAG130.13127335150964356No Hit
GTTATGGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA130.13127335150964356No Hit
TACAATGGGCTTGCCGGTTTTAATTTTAAGGAAGCATTTACATGAAAAGAAA130.13127335150964356No Hit
CGCGGGGGTGTGTTTGAGATCCCTTTTTTTCTGCAGAGGTTGTTCTTTTGGC130.13127335150964356No Hit
TGTCTGGGGGCCAGTCAGTTGCTCAAAAGGTCAATGAAAACCAAATAGTGAA120.12117540139351711No Hit
ACGAAAGGGGATATGCTGAGGTTACAGTTTGGAGGTTACATTAGAGAAAGAA120.12117540139351711No Hit
ATTAAGGGGAGAAGCTGAATGATCCATGTGTCCATTACATTGTAGTATGAGG120.12117540139351711No Hit
CACATAGGGGGGAGGGGCAAATGGCTACAGTTGTAAATGGATAACATCATTT120.12117540139351711No Hit
TGTCTAGGGATTGCACTTTTGGAGTTGTATCTAAAAAATTCTGTCTAACCCA120.12117540139351711No Hit
AGTGGGGGCACGTGGAAAGTGACCAGATGGCCGTCATAGTCCTTTTCTCTCA120.12117540139351711No Hit
ATACGTGGGTGTGAATTAAGGAATGGGTAGCGTTTACATTTAAGTACACCAA120.12117540139351711No Hit
TATCAGTGGGATGAGACTGGGGGTTAAGTTGTAACTAATATGCAAAATTGCT120.12117540139351711No Hit
TCTTTCGGGCACTAACAGGACTACATAGAGGCTTTTTCAGCATTACTGTGTC120.12117540139351711No Hit
TGAGTTGGGCGGGAAGGGGACTGCTTAAAATGTTAATGCAGTGTTTTTCTTT120.12117540139351711No Hit
CCTGATGGGGTAAATATAAAGCCTAAAGATGAAGTGGCTTCAGGAGTATAAA120.12117540139351711No Hit
TTATAAGGGAGGCTCTGAGACTAAATGAGAGGAAAAGAAAAGAAAAAAAAAT110.11107745127739069No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT110.11107745127739069No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC110.11107745127739069No Hit
GCATAGGGGTGTGATGGGCTGTGCAGAAGGGGGCTGTATCAACATCAATTAG110.11107745127739069No Hit
GAACTTGGGTAAGTACACCAATGTCTGCCCAATTTCCTAATGTATACATTTG110.11107745127739069No Hit
CGTCGAGGGACTTCTTAAAAAACATAGGTAATAGAAAATATACACAAGTCAG110.11107745127739069No Hit
TCTTAAGGGGAACAACAATAATGAAAAGAAAAACATCAAAGCCCTCTATTTT110.11107745127739069No Hit
TGTAATGGGACTACCAGCTATATTTTTAAATCCTGTTTATTTGTAAAGCTAA110.11107745127739069No Hit
GTCTCCGGGACCGCCATGGTTTCCACATTGGAAGGGCAGAACACTGTGCAGT110.11107745127739069No Hit
AATGTTGGGACTGTTAATACAGTAATGAAAAAAGACAGAACTAACTGCTAGA110.11107745127739069No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC110.11107745127739069No Hit
TGAGCGAGGGATACTCTCCAGCTCCACCGTTACCGCTAATATAAGTAAAGTT100.10097950116126427No Hit
TATATAGGGCAAAAGGAGTTCAGCTTTTATAAACACCAAAACACTCTCTGCC100.10097950116126427No Hit
ATGTTGGGGGGAGCGGCCAACATGGCGGAACGCAGGAGACACAAGAAGCGGA100.10097950116126427No Hit
CAATACGGGGATCTTCTGAGGGGATCTACGCGTGGGATTGTGTAACCTGTCA100.10097950116126427No Hit
TCGGACGGATCAAAAGGGGGTTCCAGGTTTCCATCAGTGGCCTAAAGAAGGG100.10097950116126427No Hit
TGTTATGGGGTCTTGTTCTTCATTAAAGGTAGAATTAATTTGTGTCTTTTTT100.10097950116126427No Hit
TAACAAGGGTGACCCTTCTTCCCTTATTTATTCTCTTTTCTATTTATATGTG100.10097950116126427No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT100.10097950116126427No Hit
GTTTATGGGTGAGGGGGGCATTCTTGCCTGCCAGCTGCTCCCGAGGTGGCCT100.10097950116126427No Hit
CATGCTGGGAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGG100.10097950116126427No Hit
GTAGATGGGAGATAACGTCAGTGGAGTAAATATTCGACAGGCCATAACTTGA100.10097950116126427No Hit

[FAIL]Adapter Content

Adapter graph