FastQCFastQC Report
Sat 31 Dec 2022
SRR5844564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9823
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT2252.290542604092436No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC2012.0462180596559096No Hit
CATGTGGGGCACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCA430.43774814211544333No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA370.3766670060063117No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT360.36648681665478977No Hit
CTTTGCGGGAGGCTCCTAAGTCTTATTACCAAAAATAGCTCAAACAAAAACC300.30540568054565814No Hit
ACAGTTGGGATGTAGACCCAGAAGAAGCTGTAAGGATTCACACTGATGTCCA300.30540568054565814No Hit
AACATTGGGGTCTCTGAAGTATTTCCTCCAGTTTCCCTGCGGGCCCCTATGT280.2850453018426143No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC260.26468492313957037No Hit
ACTGCAGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT250.25450473378804844No Hit
TTAAAAGGGACTATTTTATCAATCAAGGTGTTATATTTCAGTCAGAAGTGAA250.25450473378804844No Hit
TAACTGGGGATCTTCAATTAGGGATACACATACATATTAATATGCAGAGGAT240.24432454443652651No Hit
TCTTCAGGGATTTCAGGAACTGAATATTTGACTGTTAACCTTTTTCCCATAC230.23414435508500456No Hit
TAATTTGGGAGCCTTTTGGAAGTTCAAAGATGAAAGCACCATGTATCAGGAT230.23414435508500456No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA220.22396416573348266No Hit
AACACAGGAACCCGAGCCTGGCGACCATGCAGGAGTCGCTGCGGGTGAAGCA220.22396416573348266No Hit
TGCCAAGGGTCTTCAAGGGAGAAAGAAAGGGACAAGATAAAATGATAACTAA210.21378397638196067No Hit
CTGAAGGGGCATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAG210.21378397638196067No Hit
TGAGTTGGGCGGGAAGGGGACTGCTTAAAATGTTAATGCAGTGTTTTTCTTT210.21378397638196067No Hit
TCAGTAGGGCATCTGCTCTAAGGTTTCTGGAAGAGTCATTCCCAAACTTTCA190.19342359767891684No Hit
TCAGTAGGGATTTTACTTTCTCCAGTATACCTTGAGAAGCAAACTTTGTAGG190.19342359767891684No Hit
CGAACAGGGATTTTAAGGAATTGAAACCTAGCAATCTTATTGGAGAGACAAG180.18324340832739489No Hit
AGTAAAGGGGGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCCGCAGTT180.18324340832739489No Hit
GGCACTGGGGGGATCTCTTCCTTGTAAACTTGTACAGTATGTGGTGACTTTT180.18324340832739489No Hit
TATGTGGGGCGAACCGGTCGGGCTAGGAAAGAAGTAAAATATTTTGCAGAGT180.18324340832739489No Hit
GCCATTGGGGCATGAGTTCTGTTAGGAATAAAAGATGAAACTATTGTATCTC180.18324340832739489No Hit
CTCGGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC180.18324340832739489No Hit
TTTGCGGGGCTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAA170.17306321897587296No Hit
AGAAATGGGGTGCAATTTAAGACAGTTAGCAATTGCTCATTCTAAAATAAGT170.17306321897587296No Hit
GTAGTAGGGAACATTTTAATTAGTCTTTACTGTGGAAGAATGCTATTCTAAA170.17306321897587296No Hit
AATGCGGGGCTATCGAGTTAAGGAGATTGGCAGCACCATGTCTGGCAGAAAG170.17306321897587296No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT170.17306321897587296No Hit
TCCACGGGTATAATGTGATTCTTCTCAACGTGATTATTTTTTAAGAATAACA160.16288302962435103No Hit
CCAGCGTGGGACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCA160.16288302962435103No Hit
ATTAAGGGGAGAAGCTGAATGATCCATGTGTCCATTACATTGTAGTATGAGG160.16288302962435103No Hit
TTTGTCGGGCAAGCTCTGGATCTCTAATTCCATGATTAGTAATGTATGTATG160.16288302962435103No Hit
TTGACTGGGAGAAATGTGAAGTTATATTCTCAAGTTTATTTTTTTCCAAGTG160.16288302962435103No Hit
TTATAAGGGAGGCTCTGAGACTAAATGAGAGGAAAAGAAAAGAAAAAAAAAT150.15270284027282907No Hit
CTAATGAGGGGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCT150.15270284027282907No Hit
TCAATTGGGAGTGACTAATAGGGATAATCCAGGTAACTTTGAAGAGATGAGC150.15270284027282907No Hit
ATCAGGGACTGGGACTGCAGGCGTGTGCCGCCACACCTGGCTAATTTTTATT150.15270284027282907No Hit
TTTTACGGAGAGGAGAAAGCAAATAAAAATTCAAAGATATTGAGATAGTACC150.15270284027282907No Hit
TCTAACGGGTCTGGTAGAGGGAAGGGAAATGCTGTTGGTGAGAATCGACCTC150.15270284027282907No Hit
TCTTTCGGGCACTAACAGGACTACATAGAGGCTTTTTCAGCATTACTGTGTC150.15270284027282907No Hit
TTTCTAGGGAACCTTTGAAATAAATGGATGTAACTGTACCTTGGTACAGCTT140.14252265092130714No Hit
GCATAGGGGTGTGATGGGCTGTGCAGAAGGGGGCTGTATCAACATCAATTAG140.14252265092130714No Hit
TCAGTAGGGGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGAC140.14252265092130714No Hit
CGTGACGGGCGATCTTGATCCGGGGCCTGGTGCCACAGCCATGGTTTCCATT140.14252265092130714No Hit
AACCGAGGGTCCTCTCTGTCCTGCCTCAGGCCTAAGAAGCACTCTCCCTTGT140.14252265092130714No Hit
CATTAAGGGACTGAGTTATACATATTTGACTTGTGAACATATTTGTTATTTT130.13234246156978519No Hit
AGTACAGGGCCGATCTTGCTGGGGGCTTTTTCGTCTGGGCTGCCAACATGCC130.13234246156978519No Hit
TTAAGTGGGGGGATGTAAAATTGTAATGCGTATATGTACAAATGTACAATTT130.13234246156978519No Hit
TTAGTGGGGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGT130.13234246156978519No Hit
GAACTAGGGAGGAACTGACAGGTTGCATTAAAATATCAAATTGGTCAAATTT130.13234246156978519No Hit
GTTATGGGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTA130.13234246156978519No Hit
TACAATGGGCTTGCCGGTTTTAATTTTAAGGAAGCATTTACATGAAAAGAAA130.13234246156978519No Hit
AATCCGGGGTATCCTGAAAACCACAGAATGTTTCATTGCCAAATTCAGACAC130.13234246156978519No Hit
TTATCTGGGGTATCATGGCATTTGTCTACTTGCTTATTACATTGTCAATTAT130.13234246156978519No Hit
ACATTCGGGGTTCTGGGCAGTTATTGCTGTCCTAAGGCATTACTGTCGTTTG130.13234246156978519No Hit
CACTTTGGATGCCAAATTTAGTGTTGAAAGGCTCATTCAAATCAATTGGGAA130.13234246156978519No Hit
GTAGATGGGAGATAACGTCAGTGGAGTAAATATTCGACAGGCCATAACTTGA130.13234246156978519No Hit
CCTGATGGGGTAAATATAAAGCCTAAAGATGAAGTGGCTTCAGGAGTATAAA130.13234246156978519No Hit
TATATCTGGGGAAGTAACCCTTGGGCATGACTCCAATCCCAGAATTGCTCAC120.12216227221826326No Hit
GCGCACGGGAAGGAAGCTTATAATATAGCAAATACACACACATATGTACACA120.12216227221826326No Hit
AAGAAAGGGTCAAGAGTGTTTGTGGCATATATTTTTCATTTTATAAATATAA120.12216227221826326No Hit
TGTTATGGGGTCTTGTTCTTCATTAAAGGTAGAATTAATTTGTGTCTTTTTT120.12216227221826326No Hit
CAGTTGGGGAGAAGATGCATGCCAGAGACTTCACAGTTTCTGCTCTGCATGG120.12216227221826326No Hit
CTCGCTGGGGACTCCTCCCGCTAAGGCGGGAACACGCGGGGCCCAAGATGGC120.12216227221826326No Hit
GTGTAAGGGGCCTCCCGGGTTCATGCCATTCTCCTGCCTCAGCCTCCTGAGT120.12216227221826326No Hit
TACACCGGCCTGGGTAACATAGGGAGACCTTGTCTCTGCCATAAATTTAGGA110.11198208286674133No Hit
GAGTCAGGGAATAAACTCGTGCTCATCTGCAGCCTCCTCTGTGACTCCCCTT110.11198208286674133No Hit
CGTCGAGGGACTTCTTAAAAAACATAGGTAATAGAAAATATACACAAGTCAG110.11198208286674133No Hit
GTCTCCGGGGACTGCTACTTCCTGTCTCTGTTTCCATTATCACCCAGAGAGG110.11198208286674133No Hit
ATGCTTGGGACTGGAATGAGGCTCAACACTGAAAGTGCTGAAGCACAGGCAG110.11198208286674133No Hit
AGTGGGGGCACGTGGAAAGTGACCAGATGGCCGTCATAGTCCTTTTCTCTCA110.11198208286674133No Hit
AGTGATGGGAGTCTTTGATCTTTCTTGTAAGTGCATACAAGAAAACGCTGAC110.11198208286674133No Hit
GTCTCCGGGACCGCCATGGTTTCCACATTGGAAGGGCAGAACACTGTGCAGT110.11198208286674133No Hit
CGCGGGGGTGTGTTTGAGATCCCTTTTTTTCTGCAGAGGTTGTTCTTTTGGC110.11198208286674133No Hit
ATACTCGGGCTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGA110.11198208286674133No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC100.10180189351521939No Hit
AATCTTGGGGTGTTATTCCTTTTTCTATAAATATATTTTTAGGAAAAAAAGT100.10180189351521939No Hit
ACGAAAGGGGATATGCTGAGGTTACAGTTTGGAGGTTACATTAGAGAAAGAA100.10180189351521939No Hit
TGTCTAGGGATTGCACTTTTGGAGTTGTATCTAAAAAATTCTGTCTAACCCA100.10180189351521939No Hit
TCTTAAGGGGAACAACAATAATGAAAAGAAAAACATCAAAGCCCTCTATTTT100.10180189351521939No Hit
ACGTGCGGGCTTGGAGACCACTTTTATTTACTCATGAGAAGTGGAAGCTCCT100.10180189351521939No Hit
AATGTTGGGACTGTTAATACAGTAATGAAAAAAGACAGAACTAACTGCTAGA100.10180189351521939No Hit
TTAAATGGGTGCTGCAACTAAATAGCAAGGACAGCGGGCATGATAGTTCAAG100.10180189351521939No Hit
ATCATGGGGGCAATCATTGGTTCACTCAGAAGGCAACCGCCTATAGATGGAA100.10180189351521939No Hit
GTTAAGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT100.10180189351521939No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAACCTCGTATGC100.10180189351521939No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)