FastQCFastQC Report
Sat 31 Dec 2022
SRR5844563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9485
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT2102.214022140221402No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC1952.055877701634159No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA400.42171850289931473No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT340.3584607274644175No Hit
TTATAAGGGAGGCTCTGAGACTAAATGAGAGGAAAAGAAAAGAAAAAAAAAT300.316288877174486No Hit
TCCACGGGTATAATGTGATTCTTCTCAACGTGATTATTTTTTAAGAATAACA290.3057459146020032No Hit
CGAACAGGGATTTTAAGGAATTGAAACCTAGCAATCTTATTGGAGAGACAAG270.2846599894570374No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC270.2846599894570374No Hit
TTAGTGGGGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGT260.2741170268845545No Hit
CATGTGGGGCACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCA240.2530311017395888No Hit
GCCATTGGGGCATGAGTTCTGTTAGGAATAAAAGATGAAACTATTGTATCTC230.24248813916710596No Hit
GGCACTGGGGGGATCTCTTCCTTGTAAACTTGTACAGTATGTGGTGACTTTT230.24248813916710596No Hit
TCTTCAGGGATTTCAGGAACTGAATATTTGACTGTTAACCTTTTTCCCATAC230.24248813916710596No Hit
TTAAAAGGGACTATTTTATCAATCAAGGTGTTATATTTCAGTCAGAAGTGAA230.24248813916710596No Hit
AACACAGGAACCCGAGCCTGGCGACCATGCAGGAGTCGCTGCGGGTGAAGCA220.2319451765946231No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA200.21085925144965736No Hit
AACCGAGGGTCCTCTCTGTCCTGCCTCAGGCCTAAGAAGCACTCTCCCTTGT200.21085925144965736No Hit
CTCGGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC200.21085925144965736No Hit
ACTGCAGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT190.20031628887717448No Hit
TTTGTCGGGCAAGCTCTGGATCTCTAATTCCATGATTAGTAATGTATGTATG190.20031628887717448No Hit
CTCGCTGGGGACTCCTCCCGCTAAGGCGGGAACACGCGGGGCCCAAGATGGC190.20031628887717448No Hit
CGTCGAGGGACTTCTTAAAAAACATAGGTAATAGAAAATATACACAAGTCAG190.20031628887717448No Hit
CTTTGCGGGAGGCTCCTAAGTCTTATTACCAAAAATAGCTCAAACAAAAACC190.20031628887717448No Hit
TGAGTTGGGCGGGAAGGGGACTGCTTAAAATGTTAATGCAGTGTTTTTCTTT190.20031628887717448No Hit
AACATTGGGGTCTCTGAAGTATTTCCTCCAGTTTCCCTGCGGGCCCCTATGT190.20031628887717448No Hit
GTAGATGGGAGATAACGTCAGTGGAGTAAATATTCGACAGGCCATAACTTGA190.20031628887717448No Hit
ACAGTTGGGATGTAGACCCAGAAGAAGCTGTAAGGATTCACACTGATGTCCA190.20031628887717448No Hit
TCAGTAGGGCATCTGCTCTAAGGTTTCTGGAAGAGTCATTCCCAAACTTTCA180.18977332630469163No Hit
AATGTTGGGACTGTTAATACAGTAATGAAAAAAGACAGAACTAACTGCTAGA180.18977332630469163No Hit
ACTCATGGGAATGTAACCAGATTTTTTCCATTTTAAGTATTCATGAAAAATT180.18977332630469163No Hit
AATCCGGGGTATCCTGAAAACCACAGAATGTTTCATTGCCAAATTCAGACAC180.18977332630469163No Hit
TCAGTAGGGATTTTACTTTCTCCAGTATACCTTGAGAAGCAAACTTTGTAGG180.18977332630469163No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC170.17923036373220874No Hit
AGAAATGGGGTGCAATTTAAGACAGTTAGCAATTGCTCATTCTAAAATAAGT170.17923036373220874No Hit
CCAGCGTGGGACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCA170.17923036373220874No Hit
ACGAAAGGGGATATGCTGAGGTTACAGTTTGGAGGTTACATTAGAGAAAGAA170.17923036373220874No Hit
ATTAAGGGGAGAAGCTGAATGATCCATGTGTCCATTACATTGTAGTATGAGG170.17923036373220874No Hit
AGTAAAGGGGGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCCGCAGTT170.17923036373220874No Hit
TCAGTAGGGGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGAC170.17923036373220874No Hit
TGTCTGGGGGCCAGTCAGTTGCTCAAAAGGTCAATGAAAACCAAATAGTGAA160.16868740115972589No Hit
CTCCAAGGGGTTTTCAGTGGGACTTTTTTTACAGGATATAAGATGTGATTTC160.16868740115972589No Hit
CAGTTGGGGAGAAGATGCATGCCAGAGACTTCACAGTTTCTGCTCTGCATGG160.16868740115972589No Hit
CTAATGAGGGGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCT160.16868740115972589No Hit
GTAGTAGGGAACATTTTAATTAGTCTTTACTGTGGAAGAATGCTATTCTAAA160.16868740115972589No Hit
AATGCGGGGCTATCGAGTTAAGGAGATTGGCAGCACCATGTCTGGCAGAAAG150.158144438587243No Hit
TAACTGGGGATCTTCAATTAGGGATACACATACATATTAATATGCAGAGGAT150.158144438587243No Hit
CGCGGGGGTGTGTTTGAGATCCCTTTTTTTCTGCAGAGGTTGTTCTTTTGGC150.158144438587243No Hit
TCAATTGGGAGTGACTAATAGGGATAATCCAGGTAACTTTGAAGAGATGAGC150.158144438587243No Hit
ATCAGGGACTGGGACTGCAGGCGTGTGCCGCCACACCTGGCTAATTTTTATT140.14760147601476015No Hit
GAGTCAGGGAATAAACTCGTGCTCATCTGCAGCCTCCTCTGTGACTCCCCTT140.14760147601476015No Hit
CTGAAGGGGCATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAG140.14760147601476015No Hit
TAATTTGGGAGCCTTTTGGAAGTTCAAAGATGAAAGCACCATGTATCAGGAT140.14760147601476015No Hit
CCTGATGGGGTAAATATAAAGCCTAAAGATGAAGTGGCTTCAGGAGTATAAA140.14760147601476015No Hit
AGTGGGGGCACGTGGAAAGTGACCAGATGGCCGTCATAGTCCTTTTCTCTCA130.13705851344227726No Hit
TGTAATGGGACTACCAGCTATATTTTTAAATCCTGTTTATTTGTAAAGCTAA130.13705851344227726No Hit
TGCCAAGGGTCTTCAAGGGAGAAAGAAAGGGACAAGATAAAATGATAACTAA130.13705851344227726No Hit
TTTGCGGGGCTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAA130.13705851344227726No Hit
GCATAGGGGTGTGATGGGCTGTGCAGAAGGGGGCTGTATCAACATCAATTAG130.13705851344227726No Hit
ATGTTGGGGGGAGCGGCCAACATGGCGGAACGCAGGAGACACAAGAAGCGGA130.13705851344227726No Hit
ACATTCGGGGTTCTGGGCAGTTATTGCTGTCCTAAGGCATTACTGTCGTTTG130.13705851344227726No Hit
GTTAAGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT130.13705851344227726No Hit
TTTTACGGAGAGGAGAAAGCAAATAAAAATTCAAAGATATTGAGATAGTACC120.1265155508697944No Hit
TTGACTGGGAGAAATGTGAAGTTATATTCTCAAGTTTATTTTTTTCCAAGTG120.1265155508697944No Hit
TCTAACGGGTCTGGTAGAGGGAAGGGAAATGCTGTTGGTGAGAATCGACCTC120.1265155508697944No Hit
GTCTCCGGGACCGCCATGGTTTCCACATTGGAAGGGCAGAACACTGTGCAGT120.1265155508697944No Hit
TACAATGGGCTTGCCGGTTTTAATTTTAAGGAAGCATTTACATGAAAAGAAA120.1265155508697944No Hit
CAGCATGGGGATGTAACAGCCACAACTATAAAACTACTAGAAATAAACACAG120.1265155508697944No Hit
TCTTTCGGGCACTAACAGGACTACATAGAGGCTTTTTCAGCATTACTGTGTC120.1265155508697944No Hit
ACGTGCGGGCTTGGAGACCACTTTTATTTACTCATGAGAAGTGGAAGCTCCT120.1265155508697944No Hit
ATACGTGGGTGTGAATTAAGGAATGGGTAGCGTTTACATTTAAGTACACCAA110.11597258829731155No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT110.11597258829731155No Hit
CATTAAGGGACTGAGTTATACATATTTGACTTGTGAACATATTTGTTATTTT110.11597258829731155No Hit
GCCACAGGGCTGAACGAGGCTGCCATCACCACTTTCTCTGTCCTTGGGCTCT110.11597258829731155No Hit
CATGCTGGGAGGTGAGCTGGATGTGAAGAGAGCATTTGCAAAATGGAGGCGG110.11597258829731155No Hit
TGAGCGAGGGATACTCTCCAGCTCCACCGTTACCGCTAATATAAGTAAAGTT100.10542962572482868No Hit
AAAAAAGGGTGGCTTCAAGTGGCCTCTGTCCTTCCTTCAGGTTATCTTTGTG100.10542962572482868No Hit
TACACCGGCCTGGGTAACATAGGGAGACCTTGTCTCTGCCATAAATTTAGGA100.10542962572482868No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTAT100.10542962572482868No Hit
TAGTTAGGGCATAATACATAAAGTTCTCTGTAATTACAACTAAATTATTATG100.10542962572482868No Hit
GTTACCGGGATATCACCTCTAGATTTTGTACCTGAAGCCTTGTTTGGAATTC100.10542962572482868No Hit
TATATAGGGCAAAAGGAGTTCAGCTTTTATAAACACCAAAACACTCTCTGCC100.10542962572482868No Hit
GTCGGAGGGGCTGAAAACCAATTTGCAAATGTAAATCATAAGCTTAAAAATC100.10542962572482868No Hit
TTTCTAGGGAACCTTTGAAATAAATGGATGTAACTGTACCTTGGTACAGCTT100.10542962572482868No Hit
TCTTGGGGGAAGGAAGTAATTGAATAATTGCCCAAAGATTGTATGTATGAGG100.10542962572482868No Hit
AGTACAGGGCCGATCTTGCTGGGGGCTTTTTCGTCTGGGCTGCCAACATGCC100.10542962572482868No Hit
CACTTTGGATGCCAAATTTAGTGTTGAAAGGCTCATTCAAATCAATTGGGAA100.10542962572482868No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)