FastQCFastQC Report
Sat 31 Dec 2022
SRR5844562.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844562.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132387
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT23521.7766094858256476No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC20701.563597634208797No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3870.29232477509120985No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3520.2658871339330901No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2930.22132082455225965No Hit
TCAACAGGGGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAA2260.170711625763859No Hit
GTATATGGGAGTGCGTGGCACGGTCTTGGCTCACTGCAACCTCCACCTCCCA2200.16617945870818132No Hit
GGGACTGGGATTGGAATTACAAGACTATATTAAAAACATGTTTACAAAATTA2120.16013656930061107No Hit
TGCTTCGGGATAGTTGTAATTTTTAAAAGCCAACAGCATGGTGTTCATGAAT1960.14805079048547062No Hit
GTTAATGGGAAGATAGGTCTTCTCACAGACACACGTACCAGGAAAACTAACG1860.14049717872600784No Hit
GCACAAGGGGTGGTAAAATATGTTAATATTTTAATGACTTTTCCAAGGTTTC1820.1374757340222227No Hit
AGCTTTGGGATTTGCTGCATTTCAAGTTAATTCTCAGAAGTGAAAAAGACTT1780.13445428931843761No Hit
CCACCAGGGGAATAACTCCATGAAGTTGAAATTAACAAAGTAAATTACAAAT1760.13294356696654505No Hit
TTTAGCGGGATCTCCAAAGTAGCAGAAACAAGAAGGATATTTAAAAATGAAA1690.1276560387349211No Hit
AGCAACGGGGACGCCAACAACGCCAAGGCTGTGGTGAAGACCTTCCACGAGA1670.12614531638302856No Hit
ATACTAGGGATCACCGACACCTGCGACCGCATCAAGCAGTCCGCCAGCGGAA1640.12387923285518972No Hit
AGAAGCGGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG1640.12387923285518972No Hit
TAAAATGGGTTCAGGGGTGGCTGCTGTTCTGAGAACATTAGAACTGGGAAGA1630.12312387167924344No Hit
ATTCAAGGGAAGCCCCACATGCTTTAGCTCTTTTCCCTAATGCTCTCCCCCT1630.12312387167924344No Hit
CGTATCGGGGCATCCTGAGACCTTGTGTCCCTGAGTCTCCTTTCTGAGTGCA1600.12085778815140459No Hit
AGTGCGGGGAGGACACATACAGGGCCAACTCTGCTTTTTCACACATTCATCT1600.12085778815140459No Hit
CACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA1570.11859170462356575No Hit
TCGCCGGGGAGGGAAACAGGACCTCACCAAATGAACTAAATAAGGTACCAGG1570.11859170462356575No Hit
GTATCAGGGATAGGGCTTGTGGGTCACTTGAAAAGTACCTGTAGAAGCCCGG1560.11783634344761948No Hit
TATGCAGGGGAACATAAAAAGAAATGAGGAAAAAGCTTGAACCCTTAATTTT1550.11708098227167321No Hit
CTGCCAGGGCAATTCTCTCTGCTGCCAATCCTACCAGTGCTGCTGGGAATCC1550.11708098227167321No Hit
GACCACGGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGGTACAA1540.11632562109572692No Hit
CTTGAGGGGACCATATTTGGTGCTAAAACTATATTATGACTTCATTTATATG1540.11632562109572692No Hit
AGTCTTGGGATGTCATCAGTTAAGGCTATTTTTACTTCTTTTGTGGATCTTC1520.11481489874383437No Hit
GCAGCAGGGGTAATGGGACAAGATTCTTTAGGACTTTGTAGACCATTGTAAG1510.11405953756788809No Hit
TTTATGGGGAAGTTTTTAAATCTCAGTAAATCTCATCACTTCTCTTCAAAGC1500.11330417639194182No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1490.11254881521599552No Hit
GCTGTTGGGCGATCTTAGCACGGGTGGCCATTGGAGGGCTGCAGGTCGTCCT1480.11179345404004924No Hit
GATAGTGGGAATCTTAATATTTTTACATTATTATATTGCATTATTTATTTTT1470.11103809286410297No Hit
GTCTTGGGGAAGGGATATTAGAAAATACTTTGAGATGGATAAAAACAAAAGC1460.11028273168815671No Hit
CCTGCTGGGATTGTAGACGTGAGCCACCACACCCAGCCTAGAGGCTTAATTA1450.10952737051221041No Hit
TCTGTAGGGAAAGTTGTTTGAGGGGTCCATTTTCACTTGTGGCTTTTAGTGA1450.10952737051221041No Hit
GCTTTTGGGAACTGACCCAGGAAGGCTGTGACTAAGGTGTCTTCCTCAAACC1450.10952737051221041No Hit
ACTGTCGGGGCTGTCCTTTAACTGAGAAATGCAAGGGATTCGCAAGGGCGGG1450.10952737051221041No Hit
CACCAAGGGGAAATGAATGAGGTCTCAGGCGGCATTGGCGATACCAGGAATT1440.10877200933626413No Hit
CGCAAAGGGGACTACTAAAGTGGATGCTATATGCAGTAATTTTATAGTGTTT1430.10801664816031786No Hit
TTAATGGGGACTACCACTGAAAAACTGTTTGAACTGGTAAGGTGGCTAGAAA1430.10801664816031786No Hit
GAATAGGGGAACATTAGGAGTCCTTTCAGTAAGGAAATCTGGTATGTTTCAC1420.10726128698437158No Hit
CTGCCGGGGTGAAGCGGGGAAGACAAAGAAAAGGAAAAGGAAAAGGAAAGGA1420.10726128698437158No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1410.10650592580842529No Hit
GTAATAGGGAACGCTGAGACCTTCACCGAGTGGACCTTCCGCCAGAAGGGCA1410.10650592580842529No Hit
GATTACGGGCGATCTAAGCCGGGGGGAGAAAGGCCATATTTCTCTCTGAGGT1400.10575056463247902No Hit
TACTCTGGGGGTGGCATGGATGGATGTTGTTAGGAAAGCAGATATGACTTGA1390.10499520345653274No Hit
ACTGTAGGGGACCATAGATTTAAAAGTAAAGAAACTAAAAGTGCTTTTATTA1390.10499520345653274No Hit
ACATTTGGGACCAAAGATTCACAGTTTATGGACCAGAAGAAAAATGTGGCTT1390.10499520345653274No Hit
CACAACGGGATGTCTGTGCTGTTAGCCCGCTCCTTGGAAGTTAGACATATGA1390.10499520345653274No Hit
CGCAACGGGGATACCAATCTCATTTTTTTCTCCATTTATAAGTATGTCTTTA1380.10423984228058647No Hit
ATGAGGGGAGGTAGTAGACAGAACAATAACAGTTTCGCGTTAAGACTTTTAA1380.10423984228058647No Hit
AGCGATGGGCTTCCGATCTGGACAAGGGATAACCCTTGGTGCATTCAGCTGC1360.10272911992869391No Hit
CTCAACGGGGGGGGAGCACAGGAGGTGCTCTGCTCTCAGTGCTGGAATGAGG1360.10272911992869391No Hit
TCCGCCGGGATACCTGACAATGTACAGCTTGGCATCATAGCAGGAAAAACTG1350.10197375875274763No Hit
GCTAATGGGCATCTGGGAACCTCTAAAAATATCGAAGTCCCAGCCTGAAAGA1340.10121839757680136No Hit
GTACCAGGGATTCCAGCTTTCCTTTGTCTTTTACACCTTTTCCCATTCTTTT1340.10121839757680136No Hit
GAGAAAGGGGTTCATAGAATATGTGCATATTCACCTTTAGATATAGAGGCTG1340.10121839757680136No Hit
GCAAGAGGGATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATC1340.10121839757680136No Hit
CTTAGAGGGAGGCGCCCACCACCTCGCCTGGCTAATTTTTTGTATTTTTAGT1340.10121839757680136No Hit
CTACGCGGGTTCCTTTGATAGCTGTTAGTGACATAGTTGTAGAAATGCAGTT1330.10046303640085508No Hit
CTTCAGGGGGAGCTAATAAGCAAATATAGGAAAATAAATAACTCCTAGAGTA1330.10046303640085508No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)