FastQCFastQC Report
Sat 31 Dec 2022
SRR5844561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132454
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT23471.7719359173750886No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC21171.5982907273468523No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3760.2838721367418123No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3620.27330242952270223No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT3030.22875866338502426No Hit
TCAACAGGGGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAA2430.18345991816026697No Hit
TGCTTCGGGATAGTTGTAATTTTTAAAAGCCAACAGCATGGTGTTCATGAAT2120.16005556646080904No Hit
ATTCAAGGGAAGCCCCACATGCTTTAGCTCTTTTCCCTAATGCTCTCCCCCT1970.14873088015461972No Hit
AGCAACGGGGACGCCAACAACGCCAAGGCTGTGGTGAAGACCTTCCACGAGA1810.13665121476135111No Hit
CTGCCAGGGCAATTCTCTCTGCTGCCAATCCTACCAGTGCTGCTGGGAATCC1790.13514125658719253No Hit
AGAAGCGGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG1770.13363129841303395No Hit
AGCTTTGGGATTTGCTGCATTTCAAGTTAATTCTCAGAAGTGAAAAAGACTT1770.13363129841303395No Hit
AGTGCGGGGAGGACACATACAGGGCCAACTCTGCTTTTTCACACATTCATCT1750.13212134023887537No Hit
GGGACTGGGATTGGAATTACAAGACTATATTAAAAACATGTTTACAAAATTA1740.1313663611517961No Hit
GATTACGGGCGATCTAAGCCGGGGGGAGAAAGGCCATATTTCTCTCTGAGGT1720.12985640297763754No Hit
CGCAAAGGGGACTACTAAAGTGGATGCTATATGCAGTAATTTTATAGTGTTT1710.12910142389055823No Hit
GTATATGGGAGTGCGTGGCACGGTCTTGGCTCACTGCAACCTCCACCTCCCA1710.12910142389055823No Hit
ATACTAGGGATCACCGACACCTGCGACCGCATCAAGCAGTCCGCCAGCGGAA1700.12834644480347893No Hit
TGCTCAGGGATGTGATCAAACAAAACATCTTATTTTATTTTATTGCTTTTTA1690.12759146571639965No Hit
GCACAAGGGGTGGTAAAATATGTTAATATTTTAATGACTTTTCCAAGGTTTC1690.12759146571639965No Hit
CCACCAGGGGAATAACTCCATGAAGTTGAAATTAACAAAGTAAATTACAAAT1680.12683648662932037No Hit
GTATCAGGGATAGGGCTTGTGGGTCACTTGAAAAGTACCTGTAGAAGCCCGG1680.12683648662932037No Hit
GTCTTGGGGAAGGGATATTAGAAAATACTTTGAGATGGATAAAAACAAAAGC1650.12457154936808251No Hit
TAAAATGGGTTCAGGGGTGGCTGCTGTTCTGAGAACATTAGAACTGGGAAGA1630.12306159119392392No Hit
CTGCCGGGGTGAAGCGGGGAAGACAAAGAAAAGGAAAAGGAAAAGGAAAGGA1620.12230661210684464No Hit
TCGCCGGGGAGGGAAACAGGACCTCACCAAATGAACTAAATAAGGTACCAGG1600.12079665393268607No Hit
CGTATCGGGGCATCCTGAGACCTTGTGTCCCTGAGTCTCCTTTCTGAGTGCA1590.12004167484560677No Hit
CGCAACGGGGATACCAATCTCATTTTTTTCTCCATTTATAAGTATGTCTTTA1570.1185317166714482No Hit
GAGAAAGGGGTTCATAGAATATGTGCATATTCACCTTTAGATATAGAGGCTG1570.1185317166714482No Hit
GGCTTGGGATACTTTATTGATCAACATCCTTCATAGTGAAGACCTAGGAAAG1550.11702175849728962No Hit
GCAAGAGGGATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATC1530.11551180032313105No Hit
GCTTTTGGGAACTGACCCAGGAAGGCTGTGACTAAGGTGTCTTCCTCAAACC1530.11551180032313105No Hit
TACTCTGGGGGTGGCATGGATGGATGTTGTTAGGAAAGCAGATATGACTTGA1500.11324686306189319No Hit
CTACGCGGGTTCCTTTGATAGCTGTTAGTGACATAGTTGTAGAAATGCAGTT1490.1124918839748139No Hit
AGCGATGGGCTTCCGATCTGGACAAGGGATAACCCTTGGTGCATTCAGCTGC1480.11173690488773462No Hit
GTTAATGGGAAGATAGGTCTTCTCACAGACACACGTACCAGGAAAACTAACG1470.11098192580065531No Hit
CGGATAGGGACATGTAAGGTGAAACCCCATAAGTTGCCTATGAACTCAACAA1460.11022694671357604No Hit
GGACTTGGGCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.11022694671357604No Hit
TCGGACTGGGCATTCTGACAAAATCTACAGCAACGCTGGGGAGAAGAGCTTT1450.10947196762649675No Hit
CCTGCTGGGATTGTAGACGTGAGCCACCACACCCAGCCTAGAGGCTTAATTA1440.10871698853941747No Hit
GACCACGGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGGTACAA1420.10720703036525889No Hit
CTTGAGGGGACCATATTTGGTGCTAAAACTATATTATGACTTCATTTATATG1420.10720703036525889No Hit
GCAGCAGGGGTAATGGGACAAGATTCTTTAGGACTTTGTAGACCATTGTAAG1420.10720703036525889No Hit
TTTATGGGGAAGTTTTTAAATCTCAGTAAATCTCATCACTTCTCTTCAAAGC1410.1064520512781796No Hit
TATGCAGGGGAACATAAAAAGAAATGAGGAAAAAGCTTGAACCCTTAATTTT1400.10569707219110032No Hit
ATGGTTGGGTGAGGAGCTTATAAAAACAAGTCATCTTAAGTTCACAATTGCT1400.10569707219110032No Hit
GACCTTGGGTATCACAAAATAATTTGGGGGAAACCTTTCGTGGCTGTGCTGC1400.10569707219110032No Hit
GATAGTGGGAATCTTAATATTTTTACATTATTATATTGCATTATTTATTTTT1400.10569707219110032No Hit
TAGCGAGGGATCGCACCAAAAAACCTGTCAATTATGCCATTGCAATATTTCA1400.10569707219110032No Hit
ATGAGGGGAGGTAGTAGACAGAACAATAACAGTTTCGCGTTAAGACTTTTAA1400.10569707219110032No Hit
AAACTAGGGCAACAACTGGTTCATTAACGCCCGGAGACGCATCGTGCAACCT1380.10418711401694172No Hit
AGTCTTGGGATGTCATCAGTTAAGGCTATTTTTACTTCTTTTGTGGATCTTC1380.10418711401694172No Hit
TCCTTTGGGCATCCTTGTTCAAGCAGACACCCCCTCTTCACCATCCATCGAC1380.10418711401694172No Hit
TTTAGCGGGATCTCCAAAGTAGCAGAAACAAGAAGGATATTTAAAAATGAAA1380.10418711401694172No Hit
AACCGTGGGATGATGCCGTGGCACAGGAAGGCCAATGTCGGGTAGATGACAA1380.10418711401694172No Hit
CTCAGCGGGAACAAATTATTACAGGTATCTTTGATTCAGTTTATTATAATTA1370.10343213492986245No Hit
CACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA1370.10343213492986245No Hit
GTAATAGGGTGTCTTCCTTAGTGTTCCTTGCATTTTGGGACAGAATGGAATC1370.10343213492986245No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1360.10267715584278317No Hit
GTACCAGGGATTCCAGCTTTCCTTTGTCTTTTACACCTTTTCCCATTCTTTT1360.10267715584278317No Hit
CCAATCGGGAAATGCCTTGCTTCTGGAAGTATTTGAGTTTGTCACTCCTACT1360.10267715584278317No Hit
TAGCATGGGCATCTCCATATTGTAGATACACTGAAGCTCTGAGAAGCTAATT1360.10267715584278317No Hit
CACAACGGGATGTCTGTGCTGTTAGCCCGCTCCTTGGAAGTTAGACATATGA1360.10267715584278317No Hit
AAATACGGGAAGATGTGTGTTAAAAGAGCTAGAAACATTGGGAAAGGACTTA1350.10192217675570386No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1340.10116719766862457No Hit
ACATTTGGGACCAAAGATTCACAGTTTATGGACCAGAAGAAAAATGTGGCTT1340.10116719766862457No Hit
AAGCATGGGATGGATTTATTTTTTAAATAATGTCCACTGTTAATACAGGGAA1330.1004122185815453No Hit
TATTCTGGGATAAGGATTTGGGTTTGGAAATGGAGGGAGGAATTTTCATTGC1330.1004122185815453No Hit
AGCAGCGGGATGAGCACTCAAGGAAGCCTCTGATTTAATTATTTGCCAAGGA1330.1004122185815453No Hit
TAGTAAGGGGATAAGCACATATATAGTGGTTGAAGCCATAAATCACCCAGAG1330.1004122185815453No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)