FastQCFastQC Report
Sat 31 Dec 2022
SRR5844560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131730
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT22761.727776512563577No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC19691.4947240567828135No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC4000.30365140818340547No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3090.2345707128216807No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2700.20496470052379867No Hit
TCAACAGGGGAGATACTCAAAGTCGGACAGCCTCACCAAACAGAGCAGGAAA2630.19965080088058912No Hit
TGCTTCGGGATAGTTGTAATTTTTAAAAGCCAACAGCATGGTGTTCATGAAT2120.1609352463372049No Hit
TCGCCGGGGAGGGAAACAGGACCTCACCAAATGAACTAAATAAGGTACCAGG1960.14878919000986868No Hit
AGTGCGGGGAGGACACATACAGGGCCAACTCTGCTTTTTCACACATTCATCT1910.14499354740757608No Hit
GGGACTGGGATTGGAATTACAAGACTATATTAAAAACATGTTTACAAAATTA1900.1442344188871176No Hit
TGCTCAGGGATGTGATCAAACAAAACATCTTATTTTATTTTATTGCTTTTTA1880.14271616184620056No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1870.14195703332574205No Hit
GTATATGGGAGTGCGTGGCACGGTCTTGGCTCACTGCAACCTCCACCTCCCA1850.14043877628482504No Hit
TAAAATGGGTTCAGGGGTGGCTGCTGTTCTGAGAACATTAGAACTGGGAAGA1750.13284749108023988No Hit
AGCAACGGGGACGCCAACAACGCCAAGGCTGTGGTGAAGACCTTCCACGAGA1670.12677446291657177No Hit
GTTAATGGGAAGATAGGTCTTCTCACAGACACACGTACCAGGAAAACTAACG1650.12525620587565475No Hit
AGTCTTGGGATGTCATCAGTTAAGGCTATTTTTACTTCTTTTGTGGATCTTC1610.12221969179382071No Hit
CCACCAGGGGAATAACTCCATGAAGTTGAAATTAACAAAGTAAATTACAAAT1590.12070143475290368No Hit
AGCTTTGGGATTTGCTGCATTTCAAGTTAATTCTCAGAAGTGAAAAAGACTT1590.12070143475290368No Hit
GATTACGGGCGATCTAAGCCGGGGGGAGAAAGGCCATATTTCTCTCTGAGGT1580.11994230623244516No Hit
TTTAGCGGGATCTCCAAAGTAGCAGAAACAAGAAGGATATTTAAAAATGAAA1580.11994230623244516No Hit
ATTCAAGGGAAGCCCCACATGCTTTAGCTCTTTTCCCTAATGCTCTCCCCCT1580.11994230623244516No Hit
CACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA1570.11918317771198665No Hit
GATAGTGGGAATCTTAATATTTTTACATTATTATATTGCATTATTTATTTTT1570.11918317771198665No Hit
AGAAGCGGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG1570.11918317771198665No Hit
ACATTTGGGACCAAAGATTCACAGTTTATGGACCAGAAGAAAAATGTGGCTT1560.11842404919152813No Hit
GAGAAAGGGGTTCATAGAATATGTGCATATTCACCTTTAGATATAGAGGCTG1550.11766492067106962No Hit
AAGCATGGGATGGATTTATTTTTTAAATAATGTCCACTGTTAATACAGGGAA1540.1169057921506111No Hit
CTGCCGGGGTGAAGCGGGGAAGACAAAGAAAAGGAAAAGGAAAAGGAAAGGA1540.1169057921506111No Hit
ATACTAGGGATCACCGACACCTGCGACCGCATCAAGCAGTCCGCCAGCGGAA1510.11462840658923557No Hit
CTTGTAGGGACATATGGGGATAGTTTTATGATAAAATTGTTTTTTAAATACT1510.11462840658923557No Hit
AAACTAGGGCAACAACTGGTTCATTAACGCCCGGAGACGCATCGTGCAACCT1490.11311014954831854No Hit
TCGGACTGGGCATTCTGACAAAATCTACAGCAACGCTGGGGAGAAGAGCTTT1490.11311014954831854No Hit
GGCTTGGGATACTTTATTGATCAACATCCTTCATAGTGAAGACCTAGGAAAG1490.11311014954831854No Hit
GCTGTTGGGCGATCTTAGCACGGGTGGCCATTGGAGGGCTGCAGGTCGTCCT1490.11311014954831854No Hit
CTTCAGGGGGAGCTAATAAGCAAATATAGGAAAATAAATAACTCCTAGAGTA1480.11235102102786002No Hit
GCACAAGGGGTGGTAAAATATGTTAATATTTTAATGACTTTTCCAAGGTTTC1470.11159189250740151No Hit
ACTGTCGGGGCTGTCCTTTAACTGAGAAATGCAAGGGATTCGCAAGGGCGGG1470.11159189250740151No Hit
CACAACGGGATGTCTGTGCTGTTAGCCCGCTCCTTGGAAGTTAGACATATGA1450.11007363546648448No Hit
CGCAAAGGGGACTACTAAAGTGGATGCTATATGCAGTAATTTTATAGTGTTT1440.10931450694602596No Hit
GCAAGAGGGATGTGACTTGTATGAAACCCTGACCATCACCCAGGCAGTCATC1440.10931450694602596No Hit
TCGTTCGGGAGCTGTAAATATTGTATATTATTGATTTTTAATTTTATATAAG1440.10931450694602596No Hit
GTCTTGGGGAAGGGATATTAGAAAATACTTTGAGATGGATAAAAACAAAAGC1430.10855537842556745No Hit
GAATAGGGGAACATTAGGAGTCCTTTCAGTAAGGAAATCTGGTATGTTTCAC1430.10855537842556745No Hit
TAGTAAGGGGATAAGCACATATATAGTGGTTGAAGCCATAAATCACCCAGAG1410.10703712138465042No Hit
CTTAGAGGGAGGCGCCCACCACCTCGCCTGGCTAATTTTTTGTATTTTTAGT1410.10703712138465042No Hit
TAGCATGGGCATCTCCATATTGTAGATACACTGAAGCTCTGAGAAGCTAATT1400.1062779928641919No Hit
CACCAAGGGGAAATGAATGAGGTCTCAGGCGGCATTGGCGATACCAGGAATT1400.1062779928641919No Hit
TCTGCCGGGTGGATCCCTTCTCTTTGCCTGGGACCTTATTCTGATTAGCTAG1380.10475973582327487No Hit
GACCACGGAGAGGAGATCATGAAACAAATGCAAGATGCCAAGAAAGGTACAA1370.10400060730281636No Hit
CCTGCTGGGATTGTAGACGTGAGCCACCACACCCAGCCTAGAGGCTTAATTA1370.10400060730281636No Hit
ATGGTTGGGTGAGGAGCTTATAAAAACAAGTCATCTTAAGTTCACAATTGCT1370.10400060730281636No Hit
TAGCAATGGGACAGTAATGCTGCCGTTGTTCGGGACTTAAAGACACTTGACC1360.10324147878235784No Hit
GGACTTGGGCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.10248235026189934No Hit
TATGCAGGGGAACATAAAAAGAAATGAGGAAAAAGCTTGAACCCTTAATTTT1340.10172322174144081No Hit
TCCGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1340.10172322174144081No Hit
AGCTGGGGGTTCTTGTGAATTTACTTTGTCAGAACAAAATATTTACGCGTTG1340.10172322174144081No Hit
CCTCAAGGGATGTACAAAGCCTGAAGCAAATTCTCAGCGCTGTATTTAAGAG1330.1009640932209823No Hit
TGCATAGGGAGCTTACAACAGATTAGCAGACCTGACATATACTGAGAACGCA1320.10020496470052379No Hit
ACTGTAGGGGACCATAGATTTAAAAGTAAAGAAACTAAAAGTGCTTTTATTA1320.10020496470052379No Hit
ATGAGGGGAGGTAGTAGACAGAACAATAACAGTTTCGCGTTAAGACTTTTAA1320.10020496470052379No Hit
TAACGCGGGTAAGACTAATTTAGAGCTACAGTTTTCTTAAACAATCATGCAG1320.10020496470052379No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)