FastQCFastQC Report
Sat 31 Dec 2022
SRR5844549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51074
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC11462.2438031092140815No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT9221.8052237929279087No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT1620.3171868269569644No Hit
TTCGTTGGGGAATGAAGCCGGAATTCTTTTAGGCAATTTAAACTTTCTGAAA1600.31327094020440927No Hit
GATTACGGGCTATTCATTTGAAATAAGTTATGGCTTAAAATGCTTTTGGAGT1570.30739711007557663No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA1520.29760739319418883No Hit
TTATAATGGGAGCCATCAGATTTGATGAGGAACTTAGGAGAGAGGAGTTTTA1470.28781767631280103No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1460.2858597329365235No Hit
CAGTCCGGGATTTCTAATCAGTTGTTTTTAGTTTGCAGAAAAAACTTCAGCA1360.26628029917374785No Hit
AAGTTGGGGGTTGTAAGCTTGGGGTATGAAGGTTTGGGCCACGCCTGGGCGC1350.2643223557974703No Hit
TCGGCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1340.2623644124211928No Hit
TAGCAGGGGTACTGGGTTTCCAAGCAGAGGAGTCACATGATTACAATTGCCA1290.252574695539805No Hit
ATACATGGGATATGAACAGAGGCTTCACCCAACCTTAAACAGAACACTTCCA1270.24865880878724989No Hit
ATAAACGGGATAGTCATGGGGCTTCAGTGTCACTATTTTCTCCTTAAAGCTC1270.24865880878724989No Hit
CCATGAGGGGCTGTGAGGAGCTAGATGACACCAAGACACAGACACGTACTCT1250.24474292203469475No Hit
TATGGTGGGGTTTCTGATAAGCACTTGTGCAGCCCCATCAGCACCTCGATTT1250.24474292203469475No Hit
TATATAGGGAGATCCTTTCTACTTCTTGCAGTTTTACCAAAGATAACTTATA1240.2427849786584172No Hit
ATGTTGCGGGGATTTTGAGAATCCCCTATTTTCACTTCCAGATCTGTCAGCT1220.23886909190586206No Hit
GCGCTGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1210.23691114852958453No Hit
AATTAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1180.23103731840075187No Hit
GAGACAGGGATCCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATG1180.23103731840075187No Hit
TTCAAGGGAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATAACACAGCAA1150.22516348827191918No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1140.2232055448956416No Hit
GTAAAAGGGGATACACCGAGAGAAAAATGCAAAATATATTTGGTTCTCATTT1120.21928965814308649No Hit
GATGCAGGGGACTTGGGAAATGCTTACATTCAGGCCTTAATGTTGCATTAGC1090.21341582801425382No Hit
AATAAAGGGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCC1090.21341582801425382No Hit
GTAAGCTTTTATCTTTTAACCAACTGAACAATACACCAAAAGCAGCCTAGGG1060.20754199788542116No Hit
CGTTTGGGGATTTCTCATGTAATATAAGTTGAAAATTTTTGTGAGAAAAATC1050.2055840545091436No Hit
GTAAAGGGAAGATATTTTATGTAGCATATTAATTTTGAATGTTTTTAAATTA1030.20166816775658847No Hit
ACATAAGGGTATGTAAATGACATATTGAATTCCAAACCCAGTTATACTGATT1030.20166816775658847No Hit
ACGCTAGGGATTCTACACCCTAGTAGGCTCCCTTCCCCTACTCATCGCACTA1010.19775228100403336No Hit
ATATGCGGGAGCCCTGCCTGAATCAGTAGATACTTGAACGAGTCCCCAGTCT990.19383639425147825No Hit
GCGATAGGGGAATGGGATTTCTGAAGATTTGACTGAGGTCTCTCTTGGCCTG990.19383639425147825No Hit
AAAGATGGGGGTAGATGTGTAGGATCCAAACCACATTTGTGCCTAACTCCAA990.19383639425147825No Hit
TTACCGTGGGGTTATAATTGATAGGATACATCTTGGACATGGAATTGTTAAG980.1918784508752007No Hit
CTAACAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA970.18992050749892314No Hit
ACAAATGGGAGCCTTCTCCACTTCAAGTCAACTAGGACTCATAATAGTTACA960.1879625641226456No Hit
CAGCTAGGGATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC960.1879625641226456No Hit
GCACACTGGGAAGAGTGTTTATGGGATGAGATGTGCTACAAATGGGATAGAT950.186004620746368No Hit
TCCGGGGCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCGC950.186004620746368No Hit
TAATCAGGGATGCATATGTCATTTTGCAAAAGAACACATTTTGGAAGAACAT940.18404667737009045No Hit
GCAGTCAGTCCCTCAAGCCAGCGTTGCATGTTTGGGATGGTGGCTCCTGTTG940.18404667737009045No Hit
CACCCCGGGACTGAGAGATGACTGTATAGGACAAACTATCTGGTTTCATCCA930.1820887339938129No Hit
AATCGGGGGAAGAGGGAGAATTGTAAAAAGTAAAATAGAGTTTTCTCTTCAA920.18013079061753534No Hit
GCAACTGGGATATGGGAATTAAACAATTACGTAAATCAATAGCAAATGTTTG920.18013079061753534No Hit
CACTCGGGGGGAAAGCAACTTAGTGATACGTTTCAGGAGCAGTAACAATATT900.17621490386498023No Hit
TTAGTCGGGAACATGATTTTAAAACGTTGGAAAATTCATCAAGGTACAAATC890.17425696048870268No Hit
GATAATGGGACATTTTACAAAGTAAAAATGATGGCAAAATTTTTAGCTACCT890.17425696048870268No Hit
GTAGCGGGGCATGGCTGATCAGCTGACCGAAGAACAGATTGCTGAATTCAAG880.1722990171124251No Hit
GTGTCCGGGGAATTAATAAAGAATTTAGATCAGTACCTGGCACATAGAAAAC880.1722990171124251No Hit
TAACTAGGGGTACTAGGTGCGTTACTGGTGGAAAGTATCCAAGTTACCAGCG870.17034107373614754No Hit
CGATCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA870.17034107373614754No Hit
AGCGCAGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACA870.17034107373614754No Hit
CCTGATGGGAGCCTCCCGAGTAGCTGGGACTATAGGCGCCCCCCACCACGCT870.17034107373614754No Hit
ACACTAGGGAAATAAAGTATCATAAATGTAATAAGTAAATATTTATTTAGGA850.16642518698359243No Hit
TGCTAAGGGACTGAATCACATTTTCCTTAAAAAACAGCTGCTGACAGAAGAT840.16446724360731488No Hit
TAAAACGGGATCATTACAGACTTGCAGAAAATCTGAAGTTTGTTTAGAAAGT830.16250930023103732No Hit
GTTTCGGGGGTCCAAGAGGAGCAGATCGAAGTGGAGGAAACCATTGAGGCTG820.16055135685475977No Hit
GGAAAGGGGATTCACCAGCATAATCACTGTCTGCCTAAAATATGCAACTCTT820.16055135685475977No Hit
GTGCTGGGGTTTTCAAAGCCCCCGATAATCGCTCTTTTCCACTCCAAGATGC800.15663547010220463No Hit
TGTACAGGGTCCTCAACAAGCAAGATCTGCTCGCTGAGAAAGTCCTTGCTGG800.15663547010220463No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC790.1546775267259271No Hit
TAGTCCGGGATTGAGTGAAACAATCTTTGTACAATGACTAGTCATGCATCAT790.1546775267259271No Hit
CTATTTGGGGTACTCGGTGTGGATCGGTGGCTCCATCCTGGCCTCACTGTCC790.1546775267259271No Hit
GTCCACGGGACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGA790.1546775267259271No Hit
TTATTTGGGATCTGGCCAAAGTAAGCTTTATGTGCAAAGAAATAAGATCCTT780.15271958334964952No Hit
AATATAGGGAACCATGAAGAACATCACATGCAAAACAAAAATTAACCTCAAC780.15271958334964952No Hit
TTGTGCGGGGAGGAACTTACTGGGTAACTCTTGAATTCTCTAAGCTTGGCTA770.15076163997337197No Hit
ATTTGAGGGGACTTTAAAAAGCCATTGAGCAGTCCTCAGAGGTTATGGACTC760.14880369659709441No Hit
ATAGACGGGGGAGAAACAAACAGGAGTGTTCACTTATCTAGTGCAATATGTT750.14684575322081686No Hit
ACCACTGGAGGCATTCCCAGAGGTGGGGAAGAGAGCCTGCCCGGCCGGAGAA750.14684575322081686No Hit
GATCTAGGGATATGTCAGAACTAGGATTCAAACCCTAGGGTCACACTGCAGA740.14488780984453928No Hit
CTCAAAGGGATAGTCATGATCCTTCAGAGGGAATATGCACTGGCGAGTTTAA740.14488780984453928No Hit
ACTTTAGGGGGGAAAAATAGACAATGAAAAGGCAAGATAGTAAACTTAGGTT740.14488780984453928No Hit
GTGACTGGGACTTCACTGAATTGTGATGGTTAGAAACTTCGTGGATAGTTTG740.14488780984453928No Hit
ACGCCGGGGAACAAATTAAATTTTGTGGATTTCAAAACAATCTTTTTTATCA740.14488780984453928No Hit
AAAGTTGGGAAGAGAGTCTTCAGGATGTCTTTATTGATTATTTCTACATTTC730.14292986646826175No Hit
CTGTTTGGGAAGACAAGAATTTTTCTTAATAACAAAGGTCCCTTTATGAGTT730.14292986646826175No Hit
CGCAAATGGGATTAAAATGAATGTTCAGTTTCTAGAACCAATTGAAAATATT720.1409719230919842No Hit
CAGCCTGGGCGACAGAGTGAGGCTCTGCTTCAAAGAAAACAAAACAAAACAA720.1409719230919842No Hit
ATCTCAGGGATCCAGGAGCTCTTCAAGCGCATCTCGGAGCAGTTCACTGCCA720.1409719230919842No Hit
CCACGTGGGGAGCAGCTGGATTTTCGTGATGAAGGACGGAAGCAGCAGCGGG710.13901397971570661No Hit
TCTAGTGGGAGAGGAAGGGAATTTTCTCAAGCTGATAAAGGGCATCTGCAAA710.13901397971570661No Hit
GATTACGGGAGGGACTAACCATGATCAAATGCTTTAAGTGACATATCCAGAG710.13901397971570661No Hit
TATACAGGGCAAACACATGGAGGGGCCGGATCCAGAAAACACAAATGTGTGA710.13901397971570661No Hit
TTGCGCGGGATAGCTAAGCTGACAGGAAAAATAGAGTTTCTGTGCTGATTTT700.13705603633942906No Hit
GGGAAAGGTATGTTGCATTTTATCATATTTTTTGTTTTCTCCTATAATTGAC690.1350980929631515No Hit
GACTGAGGGAGTCTTTCTTCAGATATGTAAATACAATTAATGAGGTAATTAA690.1350980929631515No Hit
CACAATGGGGACCTCACACTTCCCATTCCATGGCCTTGTCTTGGCAGGGAGT690.1350980929631515No Hit
TAAAAAGGGAGACGGCCGTGTGCGACATCCCGCCCCGCGGCCTGAAGATGTC680.13314014958687392No Hit
GAATGTGGGCACCATCCCCACCAGCCAGCTCAAGTGCCTGAAGGAAGCCGGG680.13314014958687392No Hit
GTACACGGGGTAGATATTCTGTTTTATTTTGGTCATCTTTAGAAGTTATCAG680.13314014958687392No Hit
CAATTGGGGACTCTGATTGATTGTCATGGGCACCCACACAGATCAGGACACC680.13314014958687392No Hit
AACTTAGGGATTCTTGTGTTGCCATTATGATTTCAATAGGATTTTCTATTGG680.13314014958687392No Hit
ACATGTGGGGGACCAAGACATACAACAGTTTGCTTTCTAATGAAGTTCAGCC670.1311822062105964No Hit
CAGATAGGGATCAATGTATTAGATAAACCCAGTTTCAGAATAATAAAGAAAA660.12922426283431884No Hit
ATAATCGGGGCCAAATTTGCCTTTGTTTAGTTTCTGTTTCTTATCACACTGT660.12922426283431884No Hit
CACCACGGGAGAACCATGAATTCTGGTCACATTCCATTTCTCTCCAACATGA660.12922426283431884No Hit
TACTACGGGGCCTAGATATTATCCTACTGAAGATGTGCCTCGAAAGCTGTTG650.1272663194580413No Hit
TTCCTTGGGATAGTATAAAAATCCTTTTAAAAAAACAACAATCTGTGTGCCT650.1272663194580413No Hit
CAAAAAGGGAGCCTTCATTAATTTTGTAGCAAAATATAAAGTGATCATTATG650.1272663194580413No Hit
TGAGTAGGGATAGGAAAATAACCAAGCACAAAGTTATATTCCATCCTTTTTA640.1253083760817637No Hit
GCATTTGGGGGACTCAGCGTCAGGATCCGATGAGTAGAAACGGAGGAAGGTT640.1253083760817637No Hit
GACGTTGGGGCATCACACAGGTATTGCTTTTTAACAAGAACTGATGCTCCTT640.1253083760817637No Hit
AATCAAGGGATGACTCAGTTGCTTTCCCTGAGCAGGACGGGAGCCCCCCTGG630.12335043270548615No Hit
TAGAAAGGGAATAGACCCATATAAACATACTTCAAATGTGCAGTGATTTTCT630.12335043270548615No Hit
ATACTAGGGATTGTCGGCTGCAATGAGTGGGTCTTTTCTCTGGCCTCCCTAA630.12335043270548615No Hit
AAAGCAGGGAATGATTGCAGGTTACAGTTACTGGAGGGTATCTTTGCAGTCT630.12335043270548615No Hit
ATTCTCGGGGGGACACAAGGCCTAGCACCACATGTGTGAAGACACTGACAGC620.1213924893292086No Hit
ACTACAGGGATGGCATGTAGTGGAGTTTTTTTAACTTTAAAAACATCAAAAA610.11943454595293103No Hit
TTTTACGGGATCTTTTGAAAATTAGGTCGAGCCAAATTAGTGTGTAAACTCA610.11943454595293103No Hit
CTCACGGGGAATGTAAGGAACTTTGGAATAGGCAGAAAAGAGGACTTGGTGT610.11943454595293103No Hit
CCAACTGGGGTGCACAAGTAATGCAAATATAATTACTTTTAGTCCTGAGGAT610.11943454595293103No Hit
GCAAAAGGGCCGATCTGCTGCAGGGTACTCAAAGGTGACTTATTGGTTCACC610.11943454595293103No Hit
TATCCAGGGAGTGTCTACTATCTCAACCCTCCAAAATTTGCAGAGTGTTGGG610.11943454595293103No Hit
TTCGTGGGGTATGAGAGAGACACGCTTTCAAGTCCTTAGGTTCTGCCTCAAC600.11747660257665347No Hit
TACACCGGGATCTGATTCAGAAGCATCACTTCTTGGATAACTAGCACTGGAA600.11747660257665347No Hit
GCAACAGGGCACATCCCAGCCTCTCAGTGGTCTGCAGGTGGCTTTAGGCAGA600.11747660257665347No Hit
AGTACGGGGCAGAGTGTCGCTTAACACAAAGCACCCAACTTACACTTAGGAG600.11747660257665347No Hit
TAGTAAGGGACCCAGTGAAGAAAAAAAGAGAAAAGGTTTTGGTTAAATCTTA600.11747660257665347No Hit
TTAACCGGGATTTGAAGATTTGTACAATATTGAATATTCCCAATGAAACTAG600.11747660257665347No Hit
TATATTGGGGGAGGTATGGTATCCATTATCAACAGTGGATTCATGATTGACT600.11747660257665347No Hit
TGAGTGGGGATTCTCACTGGCTTCAGTGCTCATCTCTGTGAGGTTTCCAGAA590.11551865920037593No Hit
ACTTAGGGAGCATACAAAATGGAAGGATTTTTCATTTTCTTCTGGTTGTTTT590.11551865920037593No Hit
TTCAGGGGGAGGTGTGATAAATATTTCAAACTTTGTACCCTACAGAAAATAC590.11551865920037593No Hit
TCGTAGGGGAACCACCATTCCAACAGGTCGAGGAGAGCTTAAACACCTTCTT590.11551865920037593No Hit
AAATTTGGGAATGACCTAATTTATTAATCTGAAGATTAACCATTTTTTTGTC580.11356071582409838No Hit
GCATGTAGGGAGCAGATCCTGGGCCATCAAAGCCCAGAAGAGCACAGAGGCC580.11356071582409838No Hit
TGATAGGGGGAACATTTCAGATTTTGGATTTTCAGATCAGAGATGCTCAACC570.1116027724478208No Hit
CTAATAGGGGGCGCCATCAGAGCTGGAGCACCTTCAAATCTATGACACTGGG570.1116027724478208No Hit
TACCGTGGGACTTCTGAGGGCCAAAAAATGTGACTGACGGGAACAATTCTTA560.10964482907154324No Hit
GCTAAAGGGAGGCACTTTAAGGAGAACTAATAACTTTTTCCATGTATCAAGA560.10964482907154324No Hit
TCTTTGGGGATGGACATGGACTTAGACAAGGAATTTCTCCAGGACTTGAAGG560.10964482907154324No Hit
GAGCCTGGGACAGCTGTATGTATTTTTGAATACATATTATGATCTTGAGACT560.10964482907154324No Hit
CGTGACGGGGGAACAGGGCCGCGTGCGGGCCGCCACTGTGGGCTCCCTGGCC560.10964482907154324No Hit
GACCAGGGGGCCGTCTTGATGTTTAAAAAACCCAGGGTCTCCACCCTTCCTT560.10964482907154324No Hit
TTGCTGGGGGAATTCTGCCTCAGTAATATGCCTTTGTTTAAAATTTAAATAT560.10964482907154324No Hit
ACAAAGGGGATTTCCATTGAGCACATGTTTAGATTGAAGAAAGTGGTAGTCC560.10964482907154324No Hit
ACACACGGGATGCCATTTAGTATTTTTATAGTTTAGGAAAATATTTTCTAAG560.10964482907154324No Hit
CTATAAGGGAACTATAGAAAATTTTAAATATTGCTAATAGAGACTAAACTAG550.1076868856952657No Hit
CCATGTGGGATAAACTTCGCCTTAATTTTAATAATCAACACCCTCCTAGCCT550.1076868856952657No Hit
AGAACAGGGGTCATTTTTCAATGGCCCAGAACTATTAAAACATTTTATTAAC540.10572894231898812No Hit
GTGCAGGGGAAGATGTAAACATTAACACCTCGTGAATACAATTTAACTTCCA540.10572894231898812No Hit
GATACGGGGCCCTCATCAAAAAGTCTGAAAGAGCACAAATAAAAAAATCTAA530.10377099894271058No Hit
GTGTTTGGGGTTCCATGACACCATGTTTTGCCAAGGAAAAATAAAGAAATTC530.10377099894271058No Hit
ATTTTCGGGAACCAGAATTCAGAATTTAAATCCATACAGTCTGTCTTTGATT530.10377099894271058No Hit
TTGCGGAGGGATCACCGTGATGGTACAGATGAACTGAGATGAAACTTTTAGA530.10377099894271058No Hit
AAGTTTGGGATGACTGCACCATTTTACATTCTATCAATGTATGAGGTTTCCG530.10377099894271058No Hit
ACTCCAGGGATACAACAGGGTTTGATTGTAAGTCATTCTCTTTTCATGACAG530.10377099894271058No Hit
GTGTCCGGGACCTGCTAGTCATCTTCAGTATTCTGGAGAGGAAATATTCTTT530.10377099894271058No Hit
GGCTTAGGAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACC520.10181305556643303No Hit
CTGGATGGGCGATCTACTTAAGGGAATCCAATTCTGTGCACCTGAGGTCCAT520.10181305556643303No Hit
CTATTAGGGACAAGTCAGGAGGTTATTTTTCCAGATTGATAACCATAGAAAG520.10181305556643303No Hit
TCATAAGGGCACTTTCTCTGTTATTTAGGATTTATTCCTACTCAAAACCTGA520.10181305556643303No Hit
TGCACAGGGATTTTGAACCAATCATTTTTTTCTTTTTAATTTTTTCCAGCTA520.10181305556643303No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)