FastQCFastQC Report
Sat 31 Dec 2022
SRR5844547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20221
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC7183.55076405716829No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT5812.8732505810790765No Hit
TAACCGGAGACTTAAATAGCTCAGATAGCTAATTAGGAAATTTCAAGTTGGC1170.578606399287869No Hit
TACGCTGGGGACTCTTAGGATATTTGAGTCACTTGTGTCAACCACTAAAGTA1160.5736610454478018No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1120.5538796300875327No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT1110.5489342762474655No Hit
CTCACCGGGACAAAATGAACGCAAAGCTAAATGCATATTTTTAAGTATTATT1000.49453538400672564No Hit
GCTATTGGGATCTCCTAATGCTGTGCAAACATAGTTTACATGTATTGAAGGA1000.49453538400672564No Hit
TTGATAGGGATTATGACTAAGTGGAATTTATCATAGAGATACGTGGTAGGTT960.4747539686464567No Hit
AGTTTTGGGCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC960.4747539686464567No Hit
AAATGGGGGATGGATCTAACATCTGATTTCCCATCCCATGGCGAGAGTTAAA950.4698086148063894No Hit
TTGTTTGGGAGGAGCTTTTGTGACCCATTATTAAGCAAAATGAATAAAGTTC950.4698086148063894No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA840.41540972256564956No Hit
TTATGCGGGACTCGCTTCACCCTCCTCATCTTTGTTCTCCAAGTAAAGCCAC830.41046436872558234No Hit
TGTTAGGGAGTCTTTACATACCATGAATAAAACATTAAAACATCATATGGAG740.36595618416497705No Hit
GGTATCGGGTAGATAGAAAATATATTCGGATATACAGATCAACTATTAGAAT740.36595618416497705No Hit
CAGCAGGGGCATTCAGTTTATTTTGCAGCAGGTTTTTATTATTATTATTATT730.36101083032490977No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA700.346174768804708No Hit
TTAACAGGGAGTCATGAAATGTAGAAGACTGGAAAATTAAGACATTATGTAA670.3313387072845062No Hit
ATAGTAGGGAAGGGTCAGGAGTCAAGACTCAGACGGGACTTTCCTAGGGCTT670.3313387072845062No Hit
AACAGTGGGAGTCCTAAGGAGCTGAGGGTCTGAGGACGCAGGGAGGGTGGAG650.3214479996043717No Hit
TGTTTAGGGATGGAGGGGAAGAGAATCAATAATTAGCAAAGGTCACACAAAT610.30166658424410264No Hit
TTAGATGGGGGACATGAAAAGGATACTATTTTTAAAAATCTACAGAAATAAA560.27693981504376636No Hit
TTAGGGGGATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCT560.27693981504376636No Hit
AGTCTAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT560.27693981504376636No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC540.26704910736363185No Hit
TCTATAGGGTTTGAGAAACCTGCCTGGGCTCTGCCCTTAGTCATGACATCTC540.26704910736363185No Hit
GCCTCTGGGACGTCAATCATAGAATTGTGACGCACAGTTGGCAAAATAGTTC540.26704910736363185No Hit
TATACTGGGCTGGGGAGGCGCCACTGAACCGAGAAGGGATGGTCATCTGCTC540.26704910736363185No Hit
CCTAGGGGGGCCTGCTTCACACTTTCCTTGACCTTCTTCCTTTGTCTTCACG540.26704910736363185No Hit
ACGCACGGGCATTTGAGGAAGATAAGGCTAAAAGGATGGGCTGTTTGAAGCA530.26210375352356463No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT530.26210375352356463No Hit
AATACAGGGACCAAACTGATTTCAAAAGTACTACATTGAAAATAAACCGGTG510.2522130458434301No Hit
TTCGCTGGGCATTTCATTCTTGTCGTGTTGGCTTCCAGTCACTGGAAAAGCT490.24232233816329557No Hit
TTCGTTGGGCTATAAGCCTGGTTGTGACTCAGATCAGCTTAACTTTTTATAT490.24232233816329557No Hit
ACTTGTGGGCACACTAAATTACGCTTTTTTTCCCTTTACTTTTGCATCTATT480.23737698432322835No Hit
TGGGCAGGGCCCAGTGACATATATAGTTTTAAAATCTACAATTTTCTGATCT470.23243163048316107No Hit
TCTACTGGGGACGCTCTTCCGATCTGTATTAGGGAAAGCCTTTTCAGGCACA460.22748627664309382No Hit
CTCTCACCGGGTAGAATTGGCAGTTCTAATAAAAGTTTTTCCACTTTGTTAA460.22748627664309382No Hit
CAACAAGGGATACCAGTGTTTTAAAAGGTATTTTTATGTGTTTTTAAACACT460.22748627664309382No Hit
TGTTTCGGGATTATAATATAGTATAGTTTCGCTGCCCTAAACATCCTCTGCA450.22254092280302654No Hit
GTGCACGGGGAGGATATTGCCCTGCCATCCATGAAAACTGTTCTGGCACCAA450.22254092280302654No Hit
TTTTATGGGGATGGCAAGTAATTGGACAATTGGACAGTTTCCAGAAAGGCTT440.21759556896295928No Hit
ACGCATTGGGATCTGCAGTTTAAAAAGTAAATATATATTCTATCGTATTCAT430.21265021512289206No Hit
TTTCTTGGGACTTCATGTATATCAAGCACAGCAGTAAAACAAAAACCCATGT420.20770486128282478No Hit
GTACATGGGAGACCAAAGCACATAAATGCTAATTAGGGCTCAGAGGGGAAAT420.20770486128282478No Hit
TCCTGGGGGCCTTGAGAATAAGGCAGTTCTTGTAAGTTCAACCACTGGCTTT410.20275950744275753No Hit
CGATTAGGGATTCAGCAAAACCCCCACCTTAACCTCTTTTCTTTCTTGAGGG390.19286879976262303No Hit
ATGTCGGGATTGGCTAAGACTTCCCATTAAGATATCAAATAAGAACAATGAC390.19286879976262303No Hit
ACAACGGGGGGGACAGGCTGGACCCAGAGACTCTGGACCCGGGGCCTCCCCT390.19286879976262303No Hit
TGGTTTGGGATGGCAGCAGGCAAAGAGAACGAGAGCCAAGCAAAAGCGGAAG370.18297809208248852No Hit
ATAAGCGGGAGCCCTTGTGCATTTATTGTTTCTCCTGATTTTGTGGATTTAA360.17803273824242125No Hit
GAAACAGGGATGCTTCTTAAATCATAGTCCTCTACAACCTTGGGGAGTATGG360.17803273824242125No Hit
GTTGCTGGGATCAAAGAATTCATACTGGTGAGAAACCCTATGAGTGTAAACA360.17803273824242125No Hit
GACTAAGGGATTGAAGATTGGTCTTCTGTTGATCTAAGATGATTATTTTGTA350.173087384402354No Hit
TTGGTTGGGATCAAAGCGCATGCTGCTTCTCTTGCACTGTTTCCCTTTCGCA340.16814203056228674No Hit
ATATCGGGGTGACTTTTTTGCCCCAGGAGGCTCTGTCTGGAAACAGGCTTAG340.16814203056228674No Hit
ATAAAAGGGAAGCTACTGTTTGTTTTTCTAAAAGCAGGATTTGCTTCCGTAG330.16319667672221946No Hit
GTTATGGGGATTTCTTCTTGGATACCATCAAGTAACAGCTATTATTTGCCAT330.16319667672221946No Hit
ATCTTGGGGAGGAAGTGGGAGTGTTTTTATTGTTAGCTAATTGCTGTGACTG330.16319667672221946No Hit
TGCAGAGGGATACATGAAAAACCACTTGACAGTCGTCTTGGCCATGATTTCA330.16319667672221946No Hit
CACGTAGGGCAAGAGCTTTGCAGTCAAATCTGGCACTCCTGCTTAGTAGTTT320.1582513228821522No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA310.15330596904208496No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC310.15330596904208496No Hit
AAGTATGGGGAACATGGTGAAACCCCGTCTCTACTAAAGATACAAAAAATTA310.15330596904208496No Hit
GATATGGGGAATTCTAAATGAAGCTAAACACTCTGGAAAAATTCTTCAACAG310.15330596904208496No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC300.1483606152020177No Hit
TTAATTGGGATCTTAGTATACATTGCTAAATCCTCGAATATTTGGATGATTG290.14341526136195046No Hit
ATTTCTGGGAAGCACATGATAGTGGGCGATGAAGGTCAAGGAGGACTCACAG290.14341526136195046No Hit
AGTTTCGGGAAGCTGGTGAAAGTTTTAACGACTGCTGGAGGCAACATGCTTC290.14341526136195046No Hit
TTCAATGGGAGGGCCCAGGGTTGGGCCCCGCTCAAGCCACTTCCACCCTCAG280.13846990752188318No Hit
GTCAATGGGAGACGTGATTCAAAACTCTGTTCTAAGGTGTTTATTGTAGTGG280.13846990752188318No Hit
GGAATTGGGACAGCTACTTCACACCTTAAACTTGCTTTGATTTGGTGATGTA280.13846990752188318No Hit
ACACAGGGGAAATACTTTCAAGCTTCTGGTTTGGGAGTTTAGATAGGTTTGA270.13352455368181593No Hit
GTATGAGGGATCTGAGTTTTGATTTTCATTTTTTAAAAAATCAGTCAATTAT270.13352455368181593No Hit
CTCACAGGGCACACTGGTCTTGTCATTCTTTGAACATTCCAAGCCCCCTTCT270.13352455368181593No Hit
ACTATACGGGAACTCATTTGTAGTATGGCTCAAATGTGAGAAACTTGAAGGT270.13352455368181593No Hit
ACGGCCGGGCAGATTTGTGTGAATTTGAAAATAATCTGTGAAAGCTTAATCT260.12857919984174868No Hit
CATGGAGGGATTTCTAGAAAGACTTCTTTCAATAAATAATGGAATCTTAGAG260.12857919984174868No Hit
TTTTATGGGATTCTTCTTAAAGCAGGGATATGCCACATTTTATTTCTCCATC260.12857919984174868No Hit
CGCACAGGGAGCACTTTTGGAGGCTGAGACGGGAAGATCATTTGAGGCCAGG250.12363384600168141No Hit
CAGCAAGGGATTGCTCTTCATAGTCTGAAGGTGCATGAAATGGTCAACTTTC250.12363384600168141No Hit
TAACGCGGGGGGGCCTGAAGAAGAAAATGAAGAATTGGGAAAGCTTTTTTTG250.12363384600168141No Hit
CGAACCGGGATCCAGGGACCAGGAGTGACTCTCAGGTTTTGTTCTTCTCGAG250.12363384600168141No Hit
GTTTCAGGGGTTCTCACCCTCTAAGCTGCATTGAGAAATGACTCGTCTCTGT240.11868849216161417No Hit
GCCCTCGGGATCTCTCTGGGTTCTTGCCCTCTTTGGAGCTCGATGCACTACC240.11868849216161417No Hit
AACATAGGGATGTCTTTTGCTTGATAGCTCATTTCCTTTTATTATTAAATAG240.11868849216161417No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA240.11868849216161417No Hit
ATCCTAGGGATATAAAGACCCAGTACAGTGCTGTGGCTCACGCCTGTAATCC240.11868849216161417No Hit
GAGGCAGGTAGATCACCTGAGGTCACGAGTTCAAGACCAGCCTAACCAACAT240.11868849216161417No Hit
AAGCTAGGGGGCGGGGGGACAAGACAAAGCTCCCGATGCTGCATGGGGCGCT240.11868849216161417No Hit
CTCTTTGGGCCGTGAAGGTCTACTTCAGACTTCCTCGCCCTCCTCCACCCGA230.11374313832154691No Hit
TAAAAAGGGTAGTAATTTCTTCTCAGGTATGGAACCACGGTCATAACTAACA230.11374313832154691No Hit
GTATCAGGGATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTAGG230.11374313832154691No Hit
TGTTTTGGGCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC230.11374313832154691No Hit
TTTCTGGGGGAGGGAGAATGAATTGTCATGAAAAATTGGGGTTGATTTTATG220.10879778448147964No Hit
ACTGGGATATCTAAGTCAGCATCGGAGAATTCACAGTGGAGAGAAACCTTTT220.10879778448147964No Hit
GTTGGCGGGAAGGAAAAACAAAACAAAACAAACTGTCAACAGAAAATGCTGG220.10879778448147964No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAACCTCGTATGC220.10879778448147964No Hit
CCGTTAGGGTCATGCCTCACTTAGGAAACAGATATGAAAACTGCAGCCCTAG210.10385243064141239No Hit
GCCTTGGGGAGATCACTTGAGGCCAGGAGTTCGAGACCACCCTGGCTAACAT210.10385243064141239No Hit
CACATCGGGGAGGCACAGAAAACTGAATCTGTAGATAACGAGGGAGAATGAA210.10385243064141239No Hit
CTGTTAGGGAAGGTACGTCCATTTGGACTACAAGAAGAGCTTCCTTTAAAGT210.10385243064141239No Hit
AGATTCGGGAGAGCAAGACCCCATCTCTACAAAAAATTTAAAAAATAGTCAA210.10385243064141239No Hit
AATTTGGGGAGCTGTCAGTTCATTACAGCAGCCACTTACTCAGAGATTTCTT210.10385243064141239No Hit
AATTCAGGGATCTTCGCAATATCTGTGACTGTCATACTAATAATTTTGATTA210.10385243064141239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)