FastQCFastQC Report
Sat 31 Dec 2022
SRR5844546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19377
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC6783.498993652268153No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT5482.8280951643701298No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT1370.7070237910925324No Hit
TACGCTGGGGACTCTTAGGATATTTGAGTCACTTGTGTCAACCACTAAAGTA1280.6605769726995923No Hit
TTGTTTGGGAGGAGCTTTTGTGACCCATTATTAAGCAAAATGAATAAAGTTC1040.5367187903184187No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1030.5315580327192032No Hit
TAACCGGAGACTTAAATAGCTCAGATAGCTAATTAGGAAATTTCAAGTTGGC970.5005934871239098No Hit
GCTATTGGGATCTCCTAATGCTGTGCAAACATAGTTTACATGTATTGAAGGA950.49027197192547867No Hit
CAGCAGGGGCATTCAGTTTATTTTGCAGCAGGTTTTTATTATTATTATTATT910.4696289415286164No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA880.4541466687309697No Hit
TTATGCGGGACTCGCTTCACCCTCCTCATCTTTGTTCTCCAAGTAAAGCCAC860.44382515353253854No Hit
AAATGGGGGATGGATCTAACATCTGATTTCCCATCCCATGGCGAGAGTTAAA810.41802136553646074No Hit
TTGATAGGGATTATGACTAAGTGGAATTTATCATAGAGATACGTGGTAGGTT790.4076998503380296No Hit
CTCACCGGGACAAAATGAACGCAAAGCTAAATGCATATTTTTAAGTATTATT780.40253909273881405No Hit
AACAGTGGGAGTCCTAAGGAGCTGAGGGTCTGAGGACGCAGGGAGGGTGGAG760.39221757754038294No Hit
GGTATCGGGTAGATAGAAAATATATTCGGATATACAGATCAACTATTAGAAT730.3767353047427362No Hit
TGTTAGGGAGTCTTTACATACCATGAATAAAACATTAAAACATCATATGGAG660.34061000154822724No Hit
AGTTTTGGGCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC660.34061000154822724No Hit
GCCTCTGGGACGTCAATCATAGAATTGTGACGCACAGTTGGCAAAATAGTTC640.33028848634979613No Hit
TCTATAGGGTTTGAGAAACCTGCCTGGGCTCTGCCCTTAGTCATGACATCTC620.319966971151365No Hit
CCTAGGGGGGCCTGCTTCACACTTTCCTTGACCTTCTTCCTTTGTCTTCACG600.3096454559529339No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA580.29932394075450275No Hit
TTAACAGGGAGTCATGAAATGTAGAAGACTGGAAAATTAAGACATTATGTAA580.29932394075450275No Hit
AGTCTAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT580.29932394075450275No Hit
GAAACAGGGATGCTTCTTAAATCATAGTCCTCTACAACCTTGGGGAGTATGG570.29416318315528717No Hit
TTCGTTGGGCTATAAGCCTGGTTGTGACTCAGATCAGCTTAACTTTTTATAT530.27352015275842495No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC500.2580378799607782No Hit
CTCTCACCGGGTAGAATTGGCAGTTCTAATAAAAGTTTTTCCACTTTGTTAA500.2580378799607782No Hit
TTAGGGGGATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCT480.2477163647623471No Hit
TGTTTAGGGATGGAGGGGAAGAGAATCAATAATTAGCAAAGGTCACACAAAT480.2477163647623471No Hit
ATAGTAGGGAAGGGTCAGGAGTCAAGACTCAGACGGGACTTTCCTAGGGCTT480.2477163647623471No Hit
GTGCACGGGGAGGATATTGCCCTGCCATCCATGAAAACTGTTCTGGCACCAA460.237394849563916No Hit
TCTACTGGGGACGCTCTTCCGATCTGTATTAGGGAAAGCCTTTTCAGGCACA430.22191257676626927No Hit
CGATTAGGGATTCAGCAAAACCCCCACCTTAACCTCTTTTCTTTCTTGAGGG420.21675181916705372No Hit
TTTTATGGGGATGGCAAGTAATTGGACAATTGGACAGTTTCCAGAAAGGCTT420.21675181916705372No Hit
CAACAAGGGATACCAGTGTTTTAAAAGGTATTTTTATGTGTTTTTAAACACT400.2064303039686226No Hit
AGTTTCGGGAAGCTGGTGAAAGTTTTAACGACTGCTGGAGGCAACATGCTTC390.20126954636940703No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT390.20126954636940703No Hit
TGTTTCGGGATTATAATATAGTATAGTTTCGCTGCCCTAAACATCCTCTGCA380.19610878877019147No Hit
TATACTGGGCTGGGGAGGCGCCACTGAACCGAGAAGGGATGGTCATCTGCTC380.19610878877019147No Hit
ACGCATTGGGATCTGCAGTTTAAAAAGTAAATATATATTCTATCGTATTCAT380.19610878877019147No Hit
GTTATGGGGATTTCTTCTTGGATACCATCAAGTAACAGCTATTATTTGCCAT370.1909480311709759No Hit
TTAGATGGGGGACATGAAAAGGATACTATTTTTAAAAATCTACAGAAATAAA360.18578727357176034No Hit
ATGTCGGGATTGGCTAAGACTTCCCATTAAGATATCAAATAAGAACAATGAC360.18578727357176034No Hit
TGGGCAGGGCCCAGTGACATATATAGTTTTAAAATCTACAATTTTCTGATCT350.18062651597254478No Hit
CATGGAGGGATTTCTAGAAAGACTTCTTTCAATAAATAATGGAATCTTAGAG350.18062651597254478No Hit
ATATCGGGGTGACTTTTTTGCCCCAGGAGGCTCTGTCTGGAAACAGGCTTAG350.18062651597254478No Hit
TGGTTTGGGATGGCAGCAGGCAAAGAGAACGAGAGCCAAGCAAAAGCGGAAG350.18062651597254478No Hit
TTCGCTGGGCATTTCATTCTTGTCGTGTTGGCTTCCAGTCACTGGAAAAGCT340.1754657583733292No Hit
ATCTTGGGGAGGAAGTGGGAGTGTTTTTATTGTTAGCTAATTGCTGTGACTG340.1754657583733292No Hit
TGGGCCGGGGTGGGCGGATCACTTGAGGCCGGCAGTTTGAGGCCGGCCTGGG330.17030500077411362No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC330.17030500077411362No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC320.16514424317489806No Hit
TCCTGGGGGCCTTGAGAATAAGGCAGTTCTTGTAAGTTCAACCACTGGCTTT320.16514424317489806No Hit
ACGCACGGGCATTTGAGGAAGATAAGGCTAAAAGGATGGGCTGTTTGAAGCA320.16514424317489806No Hit
TCTTTTGGGATGCTAACTTCTACTATAAGAGAGAATGGGAGAGAGTTCTTTC310.1599834855756825No Hit
CTCACAGGGCACACTGGTCTTGTCATTCTTTGAACATTCCAAGCCCCCTTCT310.1599834855756825No Hit
ATAAAAGGGAAGCTACTGTTTGTTTTTCTAAAAGCAGGATTTGCTTCCGTAG300.15482272797646696No Hit
AACATAGGGATGTCTTTTGCTTGATAGCTCATTTCCTTTTATTATTAAATAG300.15482272797646696No Hit
TCCAAGGGGACACCAAAGCCTGGGTATATCTTTGGCATATCCCTGTTCTTTA300.15482272797646696No Hit
AATAGGGATGCCTTATGTGACTAATCTTCAGTGATAATATTTCATCTACTGA300.15482272797646696No Hit
GTTGCTGGGATCAAAGAATTCATACTGGTGAGAAACCCTATGAGTGTAAACA300.15482272797646696No Hit
TTGGTTGGGATCAAAGCGCATGCTGCTTCTCTTGCACTGTTTCCCTTTCGCA290.14966197037725137No Hit
ACAACGGGGGGGACAGGCTGGACCCAGAGACTCTGGACCCGGGGCCTCCCCT290.14966197037725137No Hit
GTACATGGGAGACCAAAGCACATAAATGCTAATTAGGGCTCAGAGGGGAAAT290.14966197037725137No Hit
ACACAGGGGAAATACTTTCAAGCTTCTGGTTTGGGAGTTTAGATAGGTTTGA280.14450121277803582No Hit
GTATGAGGGATCTGAGTTTTGATTTTCATTTTTTAAAAAATCAGTCAATTAT280.14450121277803582No Hit
CACGTAGGGCAAGAGCTTTGCAGTCAAATCTGGCACTCCTGCTTAGTAGTTT280.14450121277803582No Hit
AATACAGGGACCAAACTGATTTCAAAAGTACTACATTGAAAATAAACCGGTG280.14450121277803582No Hit
TTTAAAGGGTGGTGTGATGCTACTGTAATGTGATAAATTATTAAATTGTTGC270.13934045517882027No Hit
AATTTGGGGAGCTGTCAGTTCATTACAGCAGCCACTTACTCAGAGATTTCTT270.13934045517882027No Hit
TTCAATGGGAGGGCCCAGGGTTGGGCCCCGCTCAAGCCACTTCCACCCTCAG270.13934045517882027No Hit
GTCAATGGGAGACGTGATTCAAAACTCTGTTCTAAGGTGTTTATTGTAGTGG270.13934045517882027No Hit
GATATGGGGAATTCTAAATGAAGCTAAACACTCTGGAAAAATTCTTCAACAG270.13934045517882027No Hit
ACGGCCGGGCAGATTTGTGTGAATTTGAAAATAATCTGTGAAAGCTTAATCT260.13417969757960468No Hit
TTTTATGGGATTCTTCTTAAAGCAGGGATATGCCACATTTTATTTCTCCATC260.13417969757960468No Hit
TAAACTGGGAAGATGAATAGCCACATTAATTCAATGATTTTAAAAGACTATT260.13417969757960468No Hit
TGCAGAGGGATACATGAAAAACCACTTGACAGTCGTCTTGGCCATGATTTCA260.13417969757960468No Hit
AAGCTAGGGGGCGGGGGGACAAGACAAAGCTCCCGATGCTGCATGGGGCGCT260.13417969757960468No Hit
ACTTGTGGGCACACTAAATTACGCTTTTTTTCCCTTTACTTTTGCATCTATT260.13417969757960468No Hit
TTAATTGGGATCTTAGTATACATTGCTAAATCCTCGAATATTTGGATGATTG250.1290189399803891No Hit
GTATCAGGGATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTAGG250.1290189399803891No Hit
GGAATTGGGACAGCTACTTCACACCTTAAACTTGCTTTGATTTGGTGATGTA250.1290189399803891No Hit
AGTTATGGGACTGGTTCTGTGCCGCAGCACGCAAAATTTAGAATTCTACAGA240.12385818238117355No Hit
TGCTATAGGGCCAAATGTAATTTTTAAAAATTATTTTGGTTTGGAAAAAAAA240.12385818238117355No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA230.118697424781958No Hit
CTGTTAGGGAAGGTACGTCCATTTGGACTACAAGAAGAGCTTCCTTTAAAGT230.118697424781958No Hit
ATCCTAGGGATATAAAGACCCAGTACAGTGCTGTGGCTCACGCCTGTAATCC230.118697424781958No Hit
ACTATACGGGAACTCATTTGTAGTATGGCTCAAATGTGAGAAACTTGAAGGT230.118697424781958No Hit
CGCACAGGGAGCACTTTTGGAGGCTGAGACGGGAAGATCATTTGAGGCCAGG220.11353666718274243No Hit
GCCTTGGGGAGATCACTTGAGGCCAGGAGTTCGAGACCACCCTGGCTAACAT220.11353666718274243No Hit
TGCACAGGGGACTGAAGATGGGTTCCTCATCATCAATGAGCGGCTCCCACCG220.11353666718274243No Hit
TAAAAAGGGTAGTAATTTCTTCTCAGGTATGGAACCACGGTCATAACTAACA220.11353666718274243No Hit
GCCCTCGGGATCTCTCTGGGTTCTTGCCCTCTTTGGAGCTCGATGCACTACC210.10837590958352686No Hit
AATTAAGGGATCTGCCTTCTTGTTAAATGGTTGACTATTCTGTAGCTTGTAG210.10837590958352686No Hit
AATGGCGGGAATAAACAATTTTAAAAAGGACAAAAAAATTAATGATTGAGAA210.10837590958352686No Hit
ATTTCTGGGAAGCACATGATAGTGGGCGATGAAGGTCAAGGAGGACTCACAG210.10837590958352686No Hit
ATCTGAAGACATCGTCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGA210.10837590958352686No Hit
GAGGCTGGGCAGAGGAGGCAGAATAAGAAAACAATCACACTCATAACTCATT210.10837590958352686No Hit
TCAGACGGGCACCTCGGCTTCCCAAACTGCTGGGATTACAGGCATGAGCCAC210.10837590958352686No Hit
ACTACAGGGATTGCAACTTTATGAAATCTTTGTATGAAAACAAAAAGACTGC200.1032151519843113No Hit
ACCAAGGGATGGTGAAACACCGTTTCTACTAAAAATACAAAAATTAGCCAGG200.1032151519843113No Hit
TTTACAGGGAGTCTGTGCTTGAGCTCATTTTATTTTTCTGTTTAATCAACTT200.1032151519843113No Hit
CTCTTTGGGCCGTGAAGGTCTACTTCAGACTTCCTCGCCCTCCTCCACCCGA200.1032151519843113No Hit
GTAATAGGGATGGCAATCAAAGAGTTAAGGGCTGTGCAGTGATTTGTTTTGT200.1032151519843113No Hit
TGGCACGGGAAGAAGGAAAATAGTATTTATGATTTGTAGAATTGGTTCAACT200.1032151519843113No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)