FastQCFastQC Report
Sat 31 Dec 2022
SRR5844545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19619
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC6953.542484326418268No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT6093.1041337478974462No Hit
TACGCTGGGGACTCTTAGGATATTTGAGTCACTTGTGTCAACCACTAAAGTA1160.591263571028085No Hit
TTGTTTGGGAGGAGCTTTTGTGACCCATTATTAAGCAAAATGAATAAAGTTC1160.591263571028085No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1110.5657780722768745No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA1030.5250012742749376No Hit
TAACCGGAGACTTAAATAGCTCAGATAGCTAATTAGGAAATTTCAAGTTGGC960.4893215760232428No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT920.4689331770222743No Hit
CTCACCGGGACAAAATGAACGCAAAGCTAAATGCATATTTTTAAGTATTATT920.4689331770222743No Hit
AGTTTTGGGCTGCATTAAAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCC920.4689331770222743No Hit
GCTATTGGGATCTCCTAATGCTGTGCAAACATAGTTTACATGTATTGAAGGA890.453641877771548No Hit
AAATGGGGGATGGATCTAACATCTGATTTCCCATCCCATGGCGAGAGTTAAA840.42815637902033743No Hit
TGTTAGGGAGTCTTTACATACCATGAATAAAACATTAAAACATCATATGGAG810.41286507976961107No Hit
CAGCAGGGGCATTCAGTTTATTTTGCAGCAGGTTTTTATTATTATTATTATT800.407767980019369No Hit
GGTATCGGGTAGATAGAAAATATATTCGGATATACAGATCAACTATTAGAAT790.4026708802691269No Hit
TTATGCGGGACTCGCTTCACCCTCCTCATCTTTGTTCTCCAAGTAAAGCCAC760.38737958101840053No Hit
AACAGTGGGAGTCCTAAGGAGCTGAGGGTCTGAGGACGCAGGGAGGGTGGAG750.38228248126815845No Hit
GCCTCTGGGACGTCAATCATAGAATTGTGACGCACAGTTGGCAAAATAGTTC730.37208828176767417No Hit
TTGATAGGGATTATGACTAAGTGGAATTTATCATAGAGATACGTGGTAGGTT710.36189408226718994No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC700.35679698251694786No Hit
TCTATAGGGTTTGAGAAACCTGCCTGGGCTCTGCCCTTAGTCATGACATCTC700.35679698251694786No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA650.33131148376573727No Hit
CAACAAGGGATACCAGTGTTTTAAAAGGTATTTTTATGTGTTTTTAAACACT650.33131148376573727No Hit
AGTCTAGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCT620.31602018451501096No Hit
CCTAGGGGGGCCTGCTTCACACTTTCCTTGACCTTCTTCCTTTGTCTTCACG590.30072888526428465No Hit
TGTTTAGGGATGGAGGGGAAGAGAATCAATAATTAGCAAAGGTCACACAAAT570.2905346857638004No Hit
ACGCATTGGGATCTGCAGTTTAAAAAGTAAATATATATTCTATCGTATTCAT570.2905346857638004No Hit
CGATTAGGGATTCAGCAAAACCCCCACCTTAACCTCTTTTCTTTCTTGAGGG560.2854375860135583No Hit
TTAACAGGGAGTCATGAAATGTAGAAGACTGGAAAATTAAGACATTATGTAA560.2854375860135583No Hit
TCCTGGGGGCCTTGAGAATAAGGCAGTTCTTGTAAGTTCAACCACTGGCTTT550.2803404862633162No Hit
ATAGTAGGGAAGGGTCAGGAGTCAAGACTCAGACGGGACTTTCCTAGGGCTT550.2803404862633162No Hit
TTAGGGGGATTTGCTTCCAGGTGTAATTATTGCTTTCATTCCTTACCCCCCT540.27524338651307406No Hit
TGGGCAGGGCCCAGTGACATATATAGTTTTAAAATCTACAATTTTCTGATCT530.2701462867628319No Hit
TTTTATGGGGATGGCAAGTAATTGGACAATTGGACAGTTTCCAGAAAGGCTT510.2599520872623477No Hit
TTAGATGGGGGACATGAAAAGGATACTATTTTTAAAAATCTACAGAAATAAA500.2548549875121056No Hit
TTCGTTGGGCTATAAGCCTGGTTGTGACTCAGATCAGCTTAACTTTTTATAT490.24975788776186353No Hit
GTTGCTGGGATCAAAGAATTCATACTGGTGAGAAACCCTATGAGTGTAAACA490.24975788776186353No Hit
TCTACTGGGGACGCTCTTCCGATCTGTATTAGGGAAAGCCTTTTCAGGCACA480.2446607880116214No Hit
ATAAAAGGGAAGCTACTGTTTGTTTTTCTAAAAGCAGGATTTGCTTCCGTAG470.23956368826137928No Hit
TGTTTCGGGATTATAATATAGTATAGTTTCGCTGCCCTAAACATCCTCTGCA450.22936948876089505No Hit
CTCTCACCGGGTAGAATTGGCAGTTCTAATAAAAGTTTTTCCACTTTGTTAA450.22936948876089505No Hit
AATACAGGGACCAAACTGATTTCAAAAGTACTACATTGAAAATAAACCGGTG430.21917528926041083No Hit
ACACAGGGGAAATACTTTCAAGCTTCTGGTTTGGGAGTTTAGATAGGTTTGA420.21407818951016871No Hit
CACGTAGGGCAAGAGCTTTGCAGTCAAATCTGGCACTCCTGCTTAGTAGTTT420.21407818951016871No Hit
AGTTTCGGGAAGCTGGTGAAAGTTTTAACGACTGCTGGAGGCAACATGCTTC420.21407818951016871No Hit
GTGCACGGGGAGGATATTGCCCTGCCATCCATGAAAACTGTTCTGGCACCAA420.21407818951016871No Hit
ATGTCGGGATTGGCTAAGACTTCCCATTAAGATATCAAATAAGAACAATGAC410.20898108975992657No Hit
GTTATGGGGATTTCTTCTTGGATACCATCAAGTAACAGCTATTATTTGCCAT410.20898108975992657No Hit
ACGCACGGGCATTTGAGGAAGATAAGGCTAAAAGGATGGGCTGTTTGAAGCA390.1987868902594424No Hit
GAAACAGGGATGCTTCTTAAATCATAGTCCTCTACAACCTTGGGGAGTATGG380.19368979050920027No Hit
AATTTGGGGAGCTGTCAGTTCATTACAGCAGCCACTTACTCAGAGATTTCTT380.19368979050920027No Hit
ACAACGGGGGGGACAGGCTGGACCCAGAGACTCTGGACCCGGGGCCTCCCCT380.19368979050920027No Hit
ATCTTGGGGAGGAAGTGGGAGTGTTTTTATTGTTAGCTAATTGCTGTGACTG360.18349559100871604No Hit
TATACTGGGCTGGGGAGGCGCCACTGAACCGAGAAGGGATGGTCATCTGCTC360.18349559100871604No Hit
TTCGCTGGGCATTTCATTCTTGTCGTGTTGGCTTCCAGTCACTGGAAAAGCT350.17839849125847393No Hit
AATTCAGGGATCTTCGCAATATCTGTGACTGTCATACTAATAATTTTGATTA350.17839849125847393No Hit
ACTTGTGGGCACACTAAATTACGCTTTTTTTCCCTTTACTTTTGCATCTATT350.17839849125847393No Hit
CGCACAGGGAGCACTTTTGGAGGCTGAGACGGGAAGATCATTTGAGGCCAGG340.17330139150823182No Hit
TCTTTTGGGATGCTAACTTCTACTATAAGAGAGAATGGGAGAGAGTTCTTTC340.17330139150823182No Hit
CAGCAAGGGATTGCTCTTCATAGTCTGAAGGTGCATGAAATGGTCAACTTTC340.17330139150823182No Hit
GTACATGGGAGACCAAAGCACATAAATGCTAATTAGGGCTCAGAGGGGAAAT340.17330139150823182No Hit
ATATCGGGGTGACTTTTTTGCCCCAGGAGGCTCTGTCTGGAAACAGGCTTAG330.1682042917579897No Hit
GTATGAGGGATCTGAGTTTTGATTTTCATTTTTTAAAAAATCAGTCAATTAT320.1631071920077476No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC310.15801009225750548No Hit
TTCAATGGGAGGGCCCAGGGTTGGGCCCCGCTCAAGCCACTTCCACCCTCAG310.15801009225750548No Hit
TTGGTTGGGATCAAAGCGCATGCTGCTTCTCTTGCACTGTTTCCCTTTCGCA310.15801009225750548No Hit
TGCAGAGGGATACATGAAAAACCACTTGACAGTCGTCTTGGCCATGATTTCA310.15801009225750548No Hit
TTACGCAGGGAAGATCAGCCTGGGCAACACAGCAAGACCCCGTCTCTACTAA300.15291299250726337No Hit
CATGGAGGGATTTCTAGAAAGACTTCTTTCAATAAATAATGGAATCTTAGAG300.15291299250726337No Hit
TAACGCGGGGGGGCCTGAAGAAGAAAATGAAGAATTGGGAAAGCTTTTTTTG300.15291299250726337No Hit
TTTCTTGGGACTTCATGTATATCAAGCACAGCAGTAAAACAAAAACCCATGT280.14271879300677914No Hit
CTGTTAGGGAAGGTACGTCCATTTGGACTACAAGAAGAGCTTCCTTTAAAGT280.14271879300677914No Hit
GGAATTGGGACAGCTACTTCACACCTTAAACTTGCTTTGATTTGGTGATGTA280.14271879300677914No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT280.14271879300677914No Hit
TTTAAAGGGTGGTGTGATGCTACTGTAATGTGATAAATTATTAAATTGTTGC270.13762169325653703No Hit
TTTTATGGGATTCTTCTTAAAGCAGGGATATGCCACATTTTATTTCTCCATC270.13762169325653703No Hit
TGGTTTGGGATGGCAGCAGGCAAAGAGAACGAGAGCCAAGCAAAAGCGGAAG270.13762169325653703No Hit
GATATGGGGAATTCTAAATGAAGCTAAACACTCTGGAAAAATTCTTCAACAG270.13762169325653703No Hit
AACATAGGGATGTCTTTTGCTTGATAGCTCATTTCCTTTTATTATTAAATAG260.13252459350629492No Hit
TAAATGGGGACAGAAGTAAACATAAGCATGTTTTAACAGGTTTTTCTTTTCT260.13252459350629492No Hit
CTCACAGGGCACACTGGTCTTGTCATTCTTTGAACATTCCAAGCCCCCTTCT260.13252459350629492No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC260.13252459350629492No Hit
ATAAGCGGGAGCCCTTGTGCATTTATTGTTTCTCCTGATTTTGTGGATTTAA250.1274274937560528No Hit
GTCAATGGGAGACGTGATTCAAAACTCTGTTCTAAGGTGTTTATTGTAGTGG250.1274274937560528No Hit
TGGGCCGGGGTGGGCGGATCACTTGAGGCCGGCAGTTTGAGGCCGGCCTGGG240.1223303940058107No Hit
AATGCTGGGACATGCATTAGAAAACTGATTCAAGTCATTCTTTGTCTGTGGA240.1223303940058107No Hit
TTAATTGGGATCTTAGTATACATTGCTAAATCCTCGAATATTTGGATGATTG240.1223303940058107No Hit
GTACGAGGGAGATGCCTTTTGAATCCATTTTCCATAATTGCGGATAGTCATA240.1223303940058107No Hit
CCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTAT240.1223303940058107No Hit
ACTGGGATATCTAAGTCAGCATCGGAGAATTCACAGTGGAGAGAAACCTTTT230.11723329425556857No Hit
TAAAAAGGGTAGTAATTTCTTCTCAGGTATGGAACCACGGTCATAACTAACA230.11723329425556857No Hit
GTCACGGGGACCTCAGGACCAGCGTAAGCAAACTCCCTAGACAAGATGTGGC230.11723329425556857No Hit
GCCTTGGGGAGATCACTTGAGGCCAGGAGTTCGAGACCACCCTGGCTAACAT220.11213619450532648No Hit
TCCAAGGGGACACCAAAGCCTGGGTATATCTTTGGCATATCCCTGTTCTTTA220.11213619450532648No Hit
AATAGGGATGCCTTATGTGACTAATCTTCAGTGATAATATTTCATCTACTGA220.11213619450532648No Hit
TAGCCTGGGCAACATGGTGAAACTCCGTCTCCACTAAAATACAAAAAATTGG220.11213619450532648No Hit
GTATCAGGGATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTAGG220.11213619450532648No Hit
AAGCTAGGGGGCGGGGGGACAAGACAAAGCTCCCGATGCTGCATGGGGCGCT220.11213619450532648No Hit
TTTCTGGGGGAGGGAGAATGAATTGTCATGAAAAATTGGGGTTGATTTTATG210.10703909475508436No Hit
AATTAAGGGATCTGCCTTCTTGTTAAATGGTTGACTATTCTGTAGCTTGTAG210.10703909475508436No Hit
AACATCGGGGGCTGAATGCAAAAGCAGATACAAGATTCCAGCTTTCGTCTGT210.10703909475508436No Hit
CTCTTTGGGCCGTGAAGGTCTACTTCAGACTTCCTCGCCCTCCTCCACCCGA210.10703909475508436No Hit
ATCCTAGGGATATAAAGACCCAGTACAGTGCTGTGGCTCACGCCTGTAATCC210.10703909475508436No Hit
ACGCTTGGGAACATAGCAAGACTTCGTCTTTACAAAAAATTTTTAAAAATAT210.10703909475508436No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA200.10194199500484225No Hit
ACTAAAGGGAGTCTGGCCAACATGGTGAAACCCCGTCTCTACTAAAAATACA200.10194199500484225No Hit
GATCACGGGCTCATTTAAAAAATAAAAACCACTACTGTTACATTTTATTAAT200.10194199500484225No Hit
TTTACAGGGAGTCTGTGCTTGAGCTCATTTTATTTTTCTGTTTAATCAACTT200.10194199500484225No Hit
AGTCTGGGATCGAAGTGAGGTAGGTGCGGTATTAAAGTGAAAGGGAAGGTGA200.10194199500484225No Hit
ATCTGAAGACATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGC200.10194199500484225No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)