FastQCFastQC Report
Sat 31 Dec 2022
SRR5844544.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844544.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77807
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT28303.637204878738417No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC22622.9071934401789044No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC4070.5230891822072564No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3060.39328081020987826No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2700.3470124795969515No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2310.2968884547662807No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1820.23391211587646354No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT1330.17093577698664644No Hit
GAAACGGGAAACGAATGAATGAATGAACAAATTTAAATCAATAAATGATGAT1250.16065392573932938No Hit
CTAAAAGGGCAAAACAGTACATAATGTAGAATATGAAAAATCTCCCCTCATT1000.1285231405914635No Hit
TTTGGCGGGGAATTGAGCATCAGATATCAAGATTTTCTTTTTTGTTACATCA970.1246674463737196No Hit
GCTATACGGGGGGTGGCTGGGGATACGGTAAATTATCTGTGCAGTATGGGCT950.12209698356189032No Hit
GTGCTTGGGCTGACTGAAATGAGGAATAATTTTCACTCCTCTTGCTTTATGA930.11952652075006105No Hit
TTCTCGCGGGATCTCGAATTGTAATCCCCACGTGTCGGGGGAGAAGCCTGGT920.11824128934414642No Hit
TTTGTGGGGGAATTTAGTCAGTTGCTTTTTGTTCTTAATGTTTTCAAATGTG920.11824128934414642No Hit
TCAGTGGGGATGTCTTGAAATCAGACAGTGTGTAAGTCCTCCAACTTTGTTC880.11310036372048787No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA880.11310036372048787No Hit
GCGTTTCGGGATCAGAGGGGTTAGGCACTGTGGGGCAGGGGCAAGGAATGAC880.11310036372048787No Hit
ACAAACGGGTCCTTCCTCAGCATACAGACTTCATGCTATCTTCCAATTCCGG870.11181513231457324No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT860.1105299009086586No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTC860.1105299009086586No Hit
CAGCATGGGATCATAACCTTATAAAGTTGAAATTTATTTTAGAAGTAATCTT860.1105299009086586No Hit
CAAAAAGGGGGTACTGCGGGACCCACTGCGGATATGGCTGTCTTGGCTGGAT840.10795943809682933No Hit
TGAGCTGGGAGATTACTGATTTTTCTTTTCTGAAAATACATTTGAGTTTTAA840.10795943809682933No Hit
TAGCATGGGAGAAAATGTAGTACACGTAATAAAATGTCTATAACCTCACATC840.10795943809682933No Hit
TCTTTGGGGAGAGCAAAGACATTTGTTTCCCTCTTTTTTTCTGATCTGATTT820.10538897528500006No Hit
CGGATAGGGGTCCTGGTTAACTTCAGGAAGGCTTCTGTGTGAAATTATGCTG820.10538897528500006No Hit
TAGTAAGGGATTCAATTACAGATGAGTCACATGGATCTTCATTAAATGATAG800.10281851247317078No Hit
ACAGTTGGGGACCCTGTCTCTGATTTGGTCCTGTCTGACATCACTGCTATAT780.10024804966134152No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)