FastQCFastQC Report
Sat 31 Dec 2022
SRR5844543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75862
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT26663.51427592206902No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC21412.8222298383907622No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC4070.5365004877277161No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2680.35327304843004403No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2610.34404576731433395No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2520.3321821201655638No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1660.2188183807439825No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT1250.16477287706625188No Hit
GAAACGGGAAACGAATGAATGAATGAACAAATTTAAATCAATAAATGATGAT1170.15422741293401176No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1090.14368194880177163No Hit
TTTGGCGGGGAATTGAGCATCAGATATCAAGATTTTCTTTTTTGTTACATCA1060.1397273997521816No Hit
TTTGTGGGGGAATTTAGTCAGTTGCTTTTTGTTCTTAATGTTTTCAAATGTG1000.1318183016530015No Hit
TCTTTGGGGAGAGCAAAGACATTTGTTTCCCTCTTTTTTTCTGATCTGATTT990.13050011863647148No Hit
GCGTTTCGGGATCAGAGGGGTTAGGCACTGTGGGGCAGGGGCAAGGAATGAC970.12786375260341146No Hit
CTAGAAGGGAATCCACCACATTAATGATAAGGAGAAGAAATGTGGCCATCTC920.12127283752076139No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTC900.11863647148770136No Hit
AACTGCGGGACCTACATATACACTAAATCAAAGAGGTAAAATATCTCTACAA880.11600010545464133No Hit
GCTATACGGGGGGTGGCTGGGGATACGGTAAATTATCTGTGCAGTATGGGCT880.11600010545464133No Hit
TCAGTGGGGATGTCTTGAAATCAGACAGTGTGTAAGTCCTCCAACTTTGTTC870.11468192243811132No Hit
TAAAGCGGGAGTTCCATGAAGCTCTCCTTCCGGGCCCGGGCCTACGGCTTCA870.11468192243811132No Hit
AATACCGGGACCTGAAGACCCCCTCCCTACTCAAAATGTTATTCTCCTCCCA870.11468192243811132No Hit
ATGACAAGGGTGACTTTTCCTTTCATTTGAACACTTAGAGGCCATTTTAGCG850.11204555640505127No Hit
TGAGCTGGGAGATTACTGATTTTTCTTTTCTGAAAATACATTTGAGTTTTAA840.11072737338852126No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA840.11072737338852126No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATG830.10940919037199125No Hit
GTGCTTGGGCTGACTGAAATGAGGAATAATTTTCACTCCTCTTGCTTTATGA830.10940919037199125No Hit
ACGAGAGGGAGTTATTTTTTTTTCTTGGAAGATGTAGCGTTGAGTGATACTT820.10809100735546123No Hit
TGTCCAGGGTACTTTTAGCCCCATGCACAGTTAATTTATGGGGATTTACAAA810.10677282433893122No Hit
CAGCATGGGATCATAACCTTATAAAGTTGAAATTTATTTTAGAAGTAATCTT810.10677282433893122No Hit
TCGAGTCGGGGGCCTCGTGCTGTAGAGAGCAGATTTCTATGGTACCCTGTGT800.1054546413224012No Hit
CGGATAGGGGTCCTGGTTAACTTCAGGAAGGCTTCTGTGTGAAATTATGCTG800.1054546413224012No Hit
GGTCCAGGGGATTACTTATCTTCTTCCCCTTAACACATAGTTTGAATGAATT790.10413645830587119No Hit
TTCTCGCGGGATCTCGAATTGTAATCCCCACGTGTCGGGGGAGAAGCCTGGT790.10413645830587119No Hit
AGCAAAGGGGGATTTATTTGAAATATTTTAAAGTTCTTACTATAGGATTATT790.10413645830587119No Hit
GCTCGGGGACTTTTTGAAGATCACCATTCTAACTGGCATGAGATGGTGTCTC790.10413645830587119No Hit
CTAAAAGGGCAAAACAGTACATAATGTAGAATATGAAAAATCTCCCCTCATT770.10150009227281116No Hit
GAACCAGGGATTCTTCCCACTCTATGAATCTTTTGCGAAGCATGAAATGGGA770.10150009227281116No Hit
ATCTGGGGATACTTGTTAAAATGTGGTAAAAAAAAGTCTATTCAGGACCATT770.10150009227281116No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)