FastQCFastQC Report
Sat 31 Dec 2022
SRR5844542.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844542.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75592
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT27673.660440258228384No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC21592.856122340988464No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3800.5026986982749497No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2850.3770240237062123No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2640.3492433061699651No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2280.30161921896496985No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1880.2487035665149751No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1180.1561011747274844No Hit
GAAACGGGAAACGAATGAATGAATGAACAAATTTAAATCAATAAATGATGAT1140.15080960948248492No Hit
TCAGTGGGGATGTCTTGAAATCAGACAGTGTGTAAGTCCTCCAACTTTGTTC1130.14948671817123504No Hit
CGGATAGGGGTCCTGGTTAACTTCAGGAAGGCTTCTGTGTGAAATTATGCTG1000.13228913112498678No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT990.1309662398137369No Hit
TCTTTGGGGAGAGCAAAGACATTTGTTTCCCTCTTTTTTTCTGATCTGATTT970.12832045719123716No Hit
AGCAAAGGGGGATTTATTTGAAATATTTTAAAGTTCTTACTATAGGATTATT970.12832045719123716No Hit
GCGTTTCGGGATCAGAGGGGTTAGGCACTGTGGGGCAGGGGCAAGGAATGAC960.12699756587998728No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA940.12435178325748755No Hit
GTGCTTGGGCTGACTGAAATGAGGAATAATTTTCACTCCTCTTGCTTTATGA930.1230288919462377No Hit
TTTGGCGGGGAATTGAGCATCAGATATCAAGATTTTCTTTTTTGTTACATCA890.11773732670123822No Hit
GATTTGGGGTGCGCTGCAGTCAGAGGCCACCTCTTTCTTTGTGGGCCGGATG880.11641443538998836No Hit
TTCTCGCGGGATCTCGAATTGTAATCCCCACGTGTCGGGGGAGAAGCCTGGT880.11641443538998836No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT870.11509154407873849No Hit
GCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTAT870.11509154407873849No Hit
CAGCATGGGATCATAACCTTATAAAGTTGAAATTTATTTTAGAAGTAATCTT870.11509154407873849No Hit
TGAGCTGGGAGATTACTGATTTTTCTTTTCTGAAAATACATTTGAGTTTTAA830.10979997883373903No Hit
GCTATACGGGGGGTGGCTGGGGATACGGTAAATTATCTGTGCAGTATGGGCT830.10979997883373903No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC830.10979997883373903No Hit
CTAAAAGGGCAAAACAGTACATAATGTAGAATATGAAAAATCTCCCCTCATT800.10583130489998942No Hit
ACAGTTGGGGACCCTGTCTCTGATTTGGTCCTGTCTGACATCACTGCTATAT800.10583130489998942No Hit
AACTGCGGGACCTACATATACACTAAATCAAAGAGGTAAAATATCTCTACAA790.10450841358873955No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTC790.10450841358873955No Hit
ACAAACGGGTCCTTCCTCAGCATACAGACTTCATGCTATCTTCCAATTCCGG760.10053973965498994No Hit
TGTCCAGGGTACTTTTAGCCCCATGCACAGTTAATTTATGGGGATTTACAAA760.10053973965498994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)