FastQCFastQC Report
Sat 31 Dec 2022
SRR5844541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75887
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC21922.888505277583776No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT20292.673712230026223No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2950.3887358836164297No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2850.3755583960362117No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2740.361063159697972No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2120.2793627367006207No Hit
CATGTGGGGCACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCA1460.19239131867118214No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1400.1844848261230514No Hit
CTTTGCGGGAGGCTCCTAAGTCTTATTACCAAAAATAGCTCAAACAAAAACC1320.173942836058877No Hit
TTAAAAGGGACTATTTTATCAATCAAGGTGTTATATTTCAGTCAGAAGTGAA1220.16076534847865906No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1160.15285885593052828No Hit
ACTGCAGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT1130.1489056096564629No Hit
TGCCAAGGGTCTTCAAGGGAGAAAGAAAGGGACAAGATAAAATGATAACTAA1120.1475878608984411No Hit
TTAGTGGGGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGT1100.1449523633823975No Hit
TCTTCAGGGATTTCAGGAACTGAATATTTGACTGTTAACCTTTTTCCCATAC1100.1449523633823975No Hit
CTCGCTGGGGACTCCTCCCGCTAAGGCGGGAACACGCGGGGCCCAAGATGGC1050.13836361959228852No Hit
TCCACGGGTATAATGTGATTCTTCTCAACGTGATTATTTTTTAAGAATAACA1020.13441037331822314No Hit
ACAGTTGGGATGTAGACCCAGAAGAAGCTGTAAGGATTCACACTGATGTCCA1010.13309262456020135No Hit
TCAGTAGGGATTTTACTTTCTCCAGTATACCTTGAGAAGCAAACTTTGTAGG990.13045712704415777No Hit
CGAACAGGGATTTTAAGGAATTGAAACCTAGCAATCTTATTGGAGAGACAAG970.12782162952811418No Hit
ATCAGGGACTGGGACTGCAGGCGTGTGCCGCCACACCTGGCTAATTTTTATT960.12650388077009236No Hit
AACATTGGGGTCTCTGAAGTATTTCCTCCAGTTTCCCTGCGGGCCCCTATGT940.1238683832540488No Hit
TCAGTAGGGCATCTGCTCTAAGGTTTCTGGAAGAGTCATTCCCAAACTTTCA930.12255063449602699No Hit
CTCGGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC920.12123288573800518No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC910.1199151369799834No Hit
AGTAAAGGGGGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCCGCAGTT910.1199151369799834No Hit
TCTTTCGGGCACTAACAGGACTACATAGAGGCTTTTTCAGCATTACTGTGTC880.115961890705918No Hit
GCATAGGGGTGTGATGGGCTGTGCAGAAGGGGGCTGTATCAACATCAATTAG870.11464414194789622No Hit
GTAGTAGGGAACATTTTAATTAGTCTTTACTGTGGAAGAATGCTATTCTAAA850.11200864443185261No Hit
CTAATGAGGGGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCT830.10937314691580903No Hit
TAATTTGGGAGCCTTTTGGAAGTTCAAAGATGAAAGCACCATGTATCAGGAT830.10937314691580903No Hit
CAGTTGGGGAGAAGATGCATGCCAGAGACTTCACAGTTTCTGCTCTGCATGG820.10805539815778722No Hit
CCAGCGTGGGACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCA800.10541990064174364No Hit
AATGCGGGGCTATCGAGTTAAGGAGATTGGCAGCACCATGTCTGGCAGAAAG800.10541990064174364No Hit
TAACTGGGGATCTTCAATTAGGGATACACATACATATTAATATGCAGAGGAT790.10410215188372185No Hit
AACACAGGAACCCGAGCCTGGCGACCATGCAGGAGTCGCTGCGGGTGAAGCA790.10410215188372185No Hit
TACAATGGGCTTGCCGGTTTTAATTTTAAGGAAGCATTTACATGAAAAGAAA760.10014890560965646No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)