FastQCFastQC Report
Sat 31 Dec 2022
SRR5844540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74350
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC21182.8486886348352387No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT20112.7047747141896434No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2990.4021519838601211No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2860.3846671149966375No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2750.3698722259583053No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2150.28917283120376597No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1370.18426361802286484No Hit
ACAGTTGGGATGTAGACCCAGAAGAAGCTGTAAGGATTCACACTGATGTCCA1330.1788836583725622No Hit
CGAACAGGGATTTTAAGGAATTGAAACCTAGCAATCTTATTGGAGAGACAAG1300.17484868863483524No Hit
CATGTGGGGCACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCA1260.1694687289845326No Hit
CTTTGCGGGAGGCTCCTAAGTCTTATTACCAAAAATAGCTCAAACAAAAACC1220.16408876933423No Hit
ACTGCAGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT1120.15063887020847344No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1090.14660390047074645No Hit
CTCGGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC1040.1398789509078682No Hit
TCAGTAGGGATTTTACTTTCTCCAGTATACCTTGAGAAGCAAACTTTGTAGG1020.13718897108271688No Hit
TCTTCAGGGATTTCAGGAACTGAATATTTGACTGTTAACCTTTTTCCCATAC1000.13449899125756556No Hit
TTAAAAGGGACTATTTTATCAATCAAGGTGTTATATTTCAGTCAGAAGTGAA980.13180901143241427No Hit
TCTAACGGGTCTGGTAGAGGGAAGGGAAATGCTGTTGGTGAGAATCGACCTC970.1304640215198386No Hit
CCAGCGTGGGACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCA930.12508406186953597No Hit
TCAGTAGGGCATCTGCTCTAAGGTTTCTGGAAGAGTCATTCCCAAACTTTCA920.12373907195696032No Hit
CTGAAGGGGCATTTCCAACACTCAGCAGAATTAATCAAGCACCTAATAAAAG920.12373907195696032No Hit
CAGTTGGGGAGAAGATGCATGCCAGAGACTTCACAGTTTCTGCTCTGCATGG900.121049092131809No Hit
CTAATGAGGGGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCT900.121049092131809No Hit
CTCGCTGGGGACTCCTCCCGCTAAGGCGGGAACACGCGGGGCCCAAGATGGC890.11970410221923335No Hit
AACCGAGGGTCCTCTCTGTCCTGCCTCAGGCCTAAGAAGCACTCTCCCTTGT890.11970410221923335No Hit
TCTTTCGGGCACTAACAGGACTACATAGAGGCTTTTTCAGCATTACTGTGTC890.11970410221923335No Hit
TTAGTGGGGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGT890.11970410221923335No Hit
TTTGCGGGGCTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAA890.11970410221923335No Hit
GCCATTGGGGCATGAGTTCTGTTAGGAATAAAAGATGAAACTATTGTATCTC860.11566913248150638No Hit
AACATTGGGGTCTCTGAAGTATTTCCTCCAGTTTCCCTGCGGGCCCCTATGT860.11566913248150638No Hit
TCAGTAGGGGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGAC860.11566913248150638No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT850.11432414256893073No Hit
ATACTCGGGCTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGA840.11297915265635508No Hit
TTTGTCGGGCAAGCTCTGGATCTCTAATTCCATGATTAGTAATGTATGTATG820.11028917283120376No Hit
AACACAGGAACCCGAGCCTGGCGACCATGCAGGAGTCGCTGCGGGTGAAGCA820.11028917283120376No Hit
TGCCAAGGGTCTTCAAGGGAGAAAGAAAGGGACAAGATAAAATGATAACTAA810.1089441829186281No Hit
TCTTAAGGGGAACAACAATAATGAAAAGAAAAACATCAAAGCCCTCTATTTT810.1089441829186281No Hit
TTTTACGGAGAGGAGAAAGCAAATAAAAATTCAAAGATATTGAGATAGTACC810.1089441829186281No Hit
AGTAAAGGGGGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCCGCAGTT790.1062542030934768No Hit
TACAATGGGCTTGCCGGTTTTAATTTTAAGGAAGCATTTACATGAAAAGAAA780.10490921318090113No Hit
ATCAGGGACTGGGACTGCAGGCGTGTGCCGCCACACCTGGCTAATTTTTATT780.10490921318090113No Hit
GTAGATGGGAGATAACGTCAGTGGAGTAAATATTCGACAGGCCATAACTTGA770.10356422326832548No Hit
AATCCGGGGTATCCTGAAAACCACAGAATGTTTCATTGCCAAATTCAGACAC770.10356422326832548No Hit
ATTAAGGGGAGAAGCTGAATGATCCATGTGTCCATTACATTGTAGTATGAGG760.10221923335574984No Hit
TAATTTGGGAGCCTTTTGGAAGTTCAAAGATGAAAGCACCATGTATCAGGAT760.10221923335574984No Hit
TAACTGGGGATCTTCAATTAGGGATACACATACATATTAATATGCAGAGGAT750.10087424344317418No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)