FastQCFastQC Report
Sat 31 Dec 2022
SRR5844539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74018
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT19972.6979923802318355No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC19582.645302493987949No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2780.37558431732821745No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2770.3742332946040153No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2650.3580210219135886No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2180.2945229538760842No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1820.24588613580480423No Hit
ACTGCAGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT1270.17157988597368207No Hit
CTTTGCGGGAGGCTCCTAAGTCTTATTACCAAAAATAGCTCAAACAAAAACC1170.15806965873165987No Hit
CATGTGGGGCACCTCCTCAAAGCAATACACTGAAAATGTTTAGACGGGCTCA1070.14455943148963765No Hit
ATCAGGGACTGGGACTGCAGGCGTGTGCCGCCACACCTGGCTAATTTTTATT1070.14455943148963765No Hit
TCAGTAGGGCATCTGCTCTAAGGTTTCTGGAAGAGTCATTCCCAAACTTTCA1060.14320840876543545No Hit
TTAAAAGGGACTATTTTATCAATCAAGGTGTTATATTTCAGTCAGAAGTGAA1060.14320840876543545No Hit
CGAACAGGGATTTTAAGGAATTGAAACCTAGCAATCTTATTGGAGAGACAAG1040.14050636331703098No Hit
CTCGCTGGGGACTCCTCCCGCTAAGGCGGGAACACGCGGGGCCCAAGATGGC1020.13780431786862654No Hit
CCAGCGTGGGACATGGCTTGCTGCCTGTTGTACCGTGGTGACGTGGTTCCCA1010.13645329514442434No Hit
TGCCAAGGGTCTTCAAGGGAGAAAGAAAGGGACAAGATAAAATGATAACTAA1000.1351022724202221No Hit
ACAGTTGGGATGTAGACCCAGAAGAAGCTGTAAGGATTCACACTGATGTCCA990.13375124969601987No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT970.13104920424761546No Hit
TAATTTGGGAGCCTTTTGGAAGTTCAAAGATGAAAGCACCATGTATCAGGAT930.12564511335080655No Hit
AACACAGGAACCCGAGCCTGGCGACCATGCAGGAGTCGCTGCGGGTGAAGCA920.12429409062660435No Hit
GCCATTGGGGCATGAGTTCTGTTAGGAATAAAAGATGAAACTATTGTATCTC900.12159204517819991No Hit
TGTAATGGGACTACCAGCTATATTTTTAAATCCTGTTTATTTGTAAAGCTAA900.12159204517819991No Hit
TTTGTCGGGCAAGCTCTGGATCTCTAATTCCATGATTAGTAATGTATGTATG890.12024102245399768No Hit
TCAGTAGGGATTTTACTTTCTCCAGTATACCTTGAGAAGCAAACTTTGTAGG890.12024102245399768No Hit
CTCGGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC880.11888999972979544No Hit
AGTAAAGGGGGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCCGCAGTT880.11888999972979544No Hit
AACATTGGGGTCTCTGAAGTATTTCCTCCAGTTTCCCTGCGGGCCCCTATGT870.11753897700559324No Hit
TTTTACGGAGAGGAGAAAGCAAATAAAAATTCAAAGATATTGAGATAGTACC870.11753897700559324No Hit
TTTGCGGGGCTTTTCGCCATCTTTTGTCTTTCCGTGGAGCTGTCGCCATGAA870.11753897700559324No Hit
TCCACGGGTATAATGTGATTCTTCTCAACGTGATTATTTTTTAAGAATAACA860.11618795428139102No Hit
GTTAAGGGGACAGCGGACACCAGGACTCCAAAATGGCGTCAGTTGTACCAGT850.11483693155718878No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC830.11213488610878435No Hit
TCTTCAGGGATTTCAGGAACTGAATATTTGACTGTTAACCTTTTTCCCATAC830.11213488610878435No Hit
ATACTCGGGCTCTTTTCCTCCTTGGCTGTCTGAAGATAGATCGCCATCATGA820.11078386338458213No Hit
CTAATGAGGGGTGTTGTGAACACCACAAAGCCATGATCGCTGGCCTTGCCCT820.11078386338458213No Hit
TTAGTGGGGGGAGCTTCAATTGCCGCTCGCTTAGGAACTTATCCTGACTGGT820.11078386338458213No Hit
TCAGTAGGGGATGCTGGGTGATGCCCGGCTGGCCTCTCGCATGGCGGATGAC820.11078386338458213No Hit
TTATAAGGGAGGCTCTGAGACTAAATGAGAGGAAAAGAAAAGAAAAAAAAAT800.10808181793617769No Hit
AATCCGGGGTATCCTGAAAACCACAGAATGTTTCATTGCCAAATTCAGACAC770.10402874976357102No Hit
AACCGAGGGTCCTCTCTGTCCTGCCTCAGGCCTAAGAAGCACTCTCCCTTGT760.10267772703936881No Hit
CGTCGAGGGACTTCTTAAAAAACATAGGTAATAGAAAATATACACAAGTCAG760.10267772703936881No Hit
TAACTGGGGATCTTCAATTAGGGATACACATACATATTAATATGCAGAGGAT750.10132670431516658No Hit
ATTAAGGGGAGAAGCTGAATGATCCATGTGTCCATTACATTGTAGTATGAGG750.10132670431516658No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)