FastQCFastQC Report
Sat 31 Dec 2022
SRR5844538.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844538.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144740
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT17901.236700290175487No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC17251.1917921790797292No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3140.21694072129335362No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2690.18585049053475197No Hit
GCGCTCCGGCCTGGGCGACAGAGTGAGACTCCATCTCAAAAAAAAGGATTTA2220.1533784717424347No Hit
TCAATCGGGATTTTGGTCATTGTTCGCTGGCCATAGAATTTACTTCTATATT2150.14854221362443001No Hit
TAGTCAGGGAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCC2130.14716042559071438No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2060.14232416747270968No Hit
GTTAAAGGGGTGAGCTGGACCTAGGGCAGTGCCCGAGCCTCCCCACAGGGTC1960.13541522730413155No Hit
CCACTTGGGGAAATGCAAGGGATTCGCAAGGGCGGGAAAGAAGAAAATTTCG1910.13196075721984246No Hit
TCACTGGGGTGGTTCCAACTAATCTGAACCCTTCCACATGTCCCAGTCGCCC1910.13196075721984246No Hit
CCAAATGGAGGAGGCCGTCAAGAAGGGCTCGACCGCGGTTGGTGTTCGAGGA1890.13057896918612685No Hit
TGTTTCGGGCAGCAAGTGTTATCTTGCAGGTACAGTCAAGCTTCGCCTTCCT1870.12919718115241122No Hit
TCAGTTGGGGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCGC1860.1285062871355534No Hit
GTGCCTGGGAGGAACAGAACCTAGTTGGGAGTTGCTGCACTTTGGGCAAGAG1840.12712449910183776No Hit
GGGCAAGAGGAAAAACAACCTAAAAAGCAGCCTAAGAAGGATGAAGAGGGCC1840.12712449910183776No Hit
GCTGCTGGGATGTAAAATGCAGATCATTGACTGAACATTTGACTGTTCAAGG1840.12712449910183776No Hit
GCACAGGGGGAAATCAGAAAGTATGAGTTTCAACTTTGTTCTTTTTCAAGGT1830.12643360508497997No Hit
ACGATTGGGGGGTTTCATAAAACCTCCAACCTGCACCCCTCCCTTCTCCATC1710.1181428768826862No Hit
ATTACGGGATTATTGGATATAGTAAATTCATGTTCTGGAAGTTTGGGGCCTA1690.11676108884897057No Hit
TCCCGCGGGAAGATACAATATATGTGAAATTCTGGAGACTATTCCTTTGAGA1630.11261572474782369No Hit
CTATAGGGGAAGATGGTTGTATCTGCAAATGTCTTAGTGAGTTTCTATGAAC1620.11192483073096586No Hit
TTAGGTGGGGATCTGACTGCTGTAGCCTATAACATGACTACCATTTCTGTTC1610.11123393671410806No Hit
CGTAAAGGGGAGCTATTTTTTAAAGAACAAACTCAACATATCAGCAGCAAAT1600.11054304269725024No Hit
GTCACGGGGTATAGAAGAACAAGGAATTTTCCATTTGTTCAAGTCTATTTTG1580.10916125466353462No Hit
TAGCTTGGGAGTACCGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCG1580.10916125466353462No Hit
TGAAAAGGGAATCAGGCAAGAGAAATAAAAGGCATCAAAATTGAAAGGAAAG1580.10916125466353462No Hit
CTTAGTGGGCAAGACTGAATGAAAAGCACTTTGAACTTGTAAGGCCACATTT1560.10777946662981898No Hit
TTGGGAGGGGCCTGGATGATTCTGCACAGCTTATACCAGGCACGGATTGTGA1560.10777946662981898No Hit
TGCAAAGGGCTTAGAGAGTTGAGGATTGATTAAATTTGTGTTTCCCAGGCTC1550.10708857261296116No Hit
CATGCTGGGATAGCCATTAACTAATGGTAATTTCATAAGTTCCTCAAAGAAA1550.10708857261296116No Hit
GCTGCCGGGTATTTTAAAAAGTACTTAAGGAGGGCCTGTGTACAGAAACTCT1550.10708857261296116No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1530.10570678457924555No Hit
TAGCCTGGGGAAAATAGAATTATTATTGACATTATTTATAATTTGTTAGTGG1510.10432499654552993No Hit
GTTGGTGGGATCCATTGTCCAACACTGCTAGAAAAACTGTAGGAGGTACATG1510.10432499654552993No Hit
TATTAAGGGATTGAGTGAAATTACACTAACGTGCACCTCGTGATGTAGGAGG1500.1036341025286721No Hit
GCCTAAGGGGATCTCATTCGGGGATTCTCATCATGGAGAAACCTTTATTCCT1490.10294320851181428No Hit
TTGCTCGGGCACTTTGCCCAAATTCAACATAAGGTACAAAGAGCAGGGACTA1490.10294320851181428No Hit
GCCTTAGCCTCCCGAATATCTGGGACTACGAATATCTGGGACTGCAGGCGCC1480.10225231449495648No Hit
ATTCTTGGGATTTGAACATAGGAACTCTAATAGATGCTTTACACAAGTGTTT1470.10156142047809866No Hit
ACTTGAGGGCTAAAGATGTGGATGCCCTGCTGAAGAAGTCTGAGGCCCAGCA1460.10087052646124085No Hit
TGGTACGGGAACCTTCGACTCTCATATTAAGAATGTTTATGAAACTATTTTA1460.10087052646124085No Hit
ACAACAGGGAGAAGTATGCATAGAAAAAAGGTAAGCATTTTCCAATAGTGGT1460.10087052646124085No Hit
AGCGCAGGGCACCGTTTCAAGCCTGCCCTGTGGAGGAAATGCCAGTTTATTA1450.10017963244438305No Hit
GAACAGGGGGGGCTGTCTGATGTGTTAGAAGACGTTGAGCACTAGATGCAAA1450.10017963244438305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)