FastQCFastQC Report
Sat 31 Dec 2022
SRR5844537.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844537.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143817
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC17861.2418559697393214No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT17791.2369886731054047No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3280.228067613703526No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2990.20790309907729962No Hit
GTTAAAGGGGTGAGCTGGACCTAGGGCAGTGCCCGAGCCTCCCCACAGGGTC2010.13976094620246562No Hit
GCACAGGGGGAAATCAGAAAGTATGAGTTTCAACTTTGTTCTTTTTCAAGGT1930.13419832147798938No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA1920.13350299338742985No Hit
CCACTTGGGGAAATGCAAGGGATTCGCAAGGGCGGGAAAGAAGAAAATTTCG1910.13280766529687033No Hit
TCAATCGGGATTTTGGTCATTGTTCGCTGGCCATAGAATTTACTTCTATATT1860.12933102484407266No Hit
TCACTGGGGTGGTTCCAACTAATCTGAACCCTTCCACATGTCCCAGTCGCCC1800.1251590563007155No Hit
GCTGCTGGGATGTAAAATGCAGATCATTGACTGAACATTTGACTGTTCAAGG1780.12376840011959643No Hit
GGGCAAGAGGAAAAACAACCTAAAAAGCAGCCTAAGAAGGATGAAGAGGGCC1750.12168241584791784No Hit
CGTAAAGGGGAGCTATTTTTTAAAGAACAAACTCAACATATCAGCAGCAAAT1740.12098708775735831No Hit
CCAAATGGAGGAGGCCGTCAAGAAGGGCTCGACCGCGGTTGGTGTTCGAGGA1730.12029175966679878No Hit
TAGTCAGGGAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCC1720.11959643157623924No Hit
CTTTCCGGGGACGCTCTTCCGATCTGGCCACGGGGTGGTTTTTATGACCGGA1710.11890110348567971No Hit
GCCTAAGGGGATCTCATTCGGGGATTCTCATCATGGAGAAACCTTTATTCCT1700.11820577539512019No Hit
GTGCCTGGGAGGAACAGAACCTAGTTGGGAGTTGCTGCACTTTGGGCAAGAG1700.11820577539512019No Hit
AGCGCAGGGCACCGTTTCAAGCCTGCCCTGTGGAGGAAATGCCAGTTTATTA1700.11820577539512019No Hit
GCGCTCCGGCCTGGGCGACAGAGTGAGACTCCATCTCAAAAAAAAGGATTTA1670.11611979112344159No Hit
TGTTTCGGGCAGCAAGTGTTATCTTGCAGGTACAGTCAAGCTTCGCCTTCCT1660.11542446303288206No Hit
TGCAAAGGGCTTAGAGAGTTGAGGATTGATTAAATTTGTGTTTCCCAGGCTC1660.11542446303288206No Hit
TATTAAGGGATTGAGTGAAATTACACTAACGTGCACCTCGTGATGTAGGAGG1640.114033806851763No Hit
ATCAACGGGGATGGCTCTTGGTATAAGTCTCACTTAAGTTTCTCTGACGATT1630.11333847876120347No Hit
TAGCTTGGGAGTACCGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCG1630.11333847876120347No Hit
TCAGTTGGGGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCGC1630.11333847876120347No Hit
CATGCAGGGGGTCAAGGCCGCGCTCGCGGCGAGGGGCTGCGAGAGTGACCGC1630.11333847876120347No Hit
ACGATTGGGGGGTTTCATAAAACCTCCAACCTGCACCCCTCCCTTCTCCATC1600.11125249448952487No Hit
GCTGCCGGGTATTTTAAAAAGTACTTAAGGAGGGCCTGTGTACAGAAACTCT1600.11125249448952487No Hit
GTCACGGGGTATAGAAGAACAAGGAATTTTCCATTTGTTCAAGTCTATTTTG1590.11055716639896535No Hit
GCAACAGGGCTATCAGATTTCAGAACTACCTGTGCTTCTGATAAGCAAGACT1590.11055716639896535No Hit
TAACAGGGGATGAGGACAAAGAAACTTTTATTTTACTGTCGTAATGCCATGA1580.10986183830840582No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1570.10916651021784629No Hit
GTAGAGGGGTATGAGGTATTTCCAGAAAGCTTTGTTAGAATAATAATTAAAG1570.10916651021784629No Hit
TGCTAGGGGCTGGGCCTCTCAGGGTTTCACACGTACCTCGTCGCCTCCAACC1550.10777585403672722No Hit
GCCATGGGGGATGCATGAAAAATGCTCCACATTTAAAGGACATACCCGTGTA1540.1070805259461677No Hit
CAGTACGGGCTGGCCAATGCTTCACCAGTTAGTCATGCTCCAAGGATTGAGG1540.1070805259461677No Hit
ACTTGAGGGCTAAAGATGTGGATGCCCTGCTGAAGAAGTCTGAGGCCCAGCA1530.10638519785560817No Hit
CTTAGTGGGCAAGACTGAATGAAAAGCACTTTGAACTTGTAAGGCCACATTT1530.10638519785560817No Hit
TCCCGCGGGAAGATACAATATATGTGAAATTCTGGAGACTATTCCTTTGAGA1530.10638519785560817No Hit
GCGAAGGGGTACTTCAAAATGTTAGATTGAATTTTAAAACCTCAGAGTCACA1530.10638519785560817No Hit
AGCAGTGGGAATTCTTCATGGTGTATTTGCAAACTAACTTAACATAGATTCT1510.1049945416744891No Hit
CATGCTGGGATAGCCATTAACTAATGGTAATTTCATAAGTTCCTCAAAGAAA1510.1049945416744891No Hit
TCATGCGGGTGGACTTTATTCTCAAGCTGAAGGGAAGCTGTTGCATGGTTTT1500.10429921358392957No Hit
GGTAATGGGAGGTTAAATCACAGGTAGTTTTTAATATCCTTTTTGTACACTA1480.1029085574028105No Hit
GCCTTAGCCTCCCGAATATCTGGGACTACGAATATCTGGGACTGCAGGCGCC1480.1029085574028105No Hit
TTGGGAGGGGCCTGGATGATTCTGCACAGCTTATACCAGGCACGGATTGTGA1480.1029085574028105No Hit
GGGCATGGGTAGCCAGGGGACATTCAAATAGACTCGTATGACAATAATAATG1450.10082257313113192No Hit
CTAGCAGCGGGACAGGTGATAAGGCCAAGCAAGACAAGAGAGGAAATAGCAA1450.10082257313113192No Hit
AATTACGGGTTCTGAAGGCAGGTCTGGTAAGAATAGGTAAGTATCAGAAAGA1450.10082257313113192No Hit
AGGTGCGGGGAAAAAGGAAAGGTAATAAACAAACAACCAAAGTGATAAATGG1450.10082257313113192No Hit
GTAAACGGGCGATCTTTAGTTGGGAAGTCAAGATGAATGAGTTTGATTTTTA1440.10012724504057238No Hit
GCTTGGGTGTCCTCTCTGATTTCCTTGAGCAGTGGTTTCTAGTTCTCCTTGA1440.10012724504057238No Hit
CTCAGAGGGATATATTCTCTGAATATTGAAGATCAGATTTCTTCTATCAATA1440.10012724504057238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)