FastQCFastQC Report
Sat 31 Dec 2022
SRR5844536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143578
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT17731.2348688517739488No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC16501.1492011310925072No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2860.19919486272270123No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2700.18805109417877391No Hit
CCACTTGGGGAAATGCAAGGGATTCGCAAGGGCGGGAAAGAAGAAAATTTCG2220.15461978854699188No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA2150.14974438980902366No Hit
TCAATCGGGATTTTGGTCATTGTTCGCTGGCCATAGAATTTACTTCTATATT2040.14208304893507362No Hit
TGGTACGGGAACCTTCGACTCTCATATTAAGAATGTTTATGAAACTATTTTA2020.1406900778670827No Hit
GCACAGGGGGAAATCAGAAAGTATGAGTTTCAACTTTGTTCTTTTTCAAGGT2010.13999359233308725No Hit
TCACTGGGGTGGTTCCAACTAATCTGAACCCTTCCACATGTCCCAGTCGCCC1960.13651116466310995No Hit
GGGCAAGAGGAAAAACAACCTAAAAAGCAGCCTAAGAAGGATGAAGAGGGCC1900.13233225145913718No Hit
GCGCTCCGGCCTGGGCGACAGAGTGAGACTCCATCTCAAAAAAAAGGATTTA1850.12884982378915988No Hit
GCTGCTGGGATGTAAAATGCAGATCATTGACTGAACATTTGACTGTTCAAGG1840.12815333825516445No Hit
GTCACGGGGTATAGAAGAACAAGGAATTTTCCATTTGTTCAAGTCTATTTTG1830.127456852721169No Hit
GTGCCTGGGAGGAACAGAACCTAGTTGGGAGTTGCTGCACTTTGGGCAAGAG1790.12467091058518714No Hit
GTTAAAGGGGTGAGCTGGACCTAGGGCAGTGCCCGAGCCTCCCCACAGGGTC1760.12258145398320076No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1750.12188496844920531No Hit
TCAGTTGGGGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCGCGC1730.1204919973812144No Hit
CGTAAAGGGGAGCTATTTTTTAAAGAACAAACTCAACATATCAGCAGCAAAT1720.11979551184721894No Hit
TCATGCGGGTGGACTTTATTCTCAAGCTGAAGGGAAGCTGTTGCATGGTTTT1700.11840254077922802No Hit
CTATAGGGGAAGATGGTTGTATCTGCAAATGTCTTAGTGAGTTTCTATGAAC1690.11770605524523256No Hit
GCCATGGGGGATGCATGAAAAATGCTCCACATTTAAAGGACATACCCGTGTA1680.1170095697112371No Hit
AGCTGCGGGTATGTCTAGTCTGATACCATTTTTAAAAATAAAAGTACCAATC1660.11561659864324618No Hit
ATTCTTGGGATTTGAACATAGGAACTCTAATAGATGCTTTACACAAGTGTTT1650.11492011310925071No Hit
TATTAAGGGATTGAGTGAAATTACACTAACGTGCACCTCGTGATGTAGGAGG1650.11492011310925071No Hit
CATGTCGGGGGAGAAGTGGGGTTGCTTTCGTTGTACTTTTTTTCATTTGTTT1630.1135271420412598No Hit
TCCCGCGGGAAGATACAATATATGTGAAATTCTGGAGACTATTCCTTTGAGA1620.11283065650726433No Hit
GGGCATGGGTAGCCAGGGGACATTCAAATAGACTCGTATGACAATAATAATG1610.11213417097326889No Hit
TTCCTGGGGGTGCCATTTGAATGTCCCAGGGCTTATTAATATTGAAGATTTT1610.11213417097326889No Hit
GCCTAAGGGGATCTCATTCGGGGATTCTCATCATGGAGAAACCTTTATTCCT1600.11143768543927343No Hit
CTTAGTGGGCAAGACTGAATGAAAAGCACTTTGAACTTGTAAGGCCACATTT1600.11143768543927343No Hit
ATTACGGGATTATTGGATATAGTAAATTCATGTTCTGGAAGTTTGGGGCCTA1600.11143768543927343No Hit
TGCAAAGGGCTTAGAGAGTTGAGGATTGATTAAATTTGTGTTTCCCAGGCTC1590.11074119990527798No Hit
TTAGGTGGGGATCTGACTGCTGTAGCCTATAACATGACTACCATTTCTGTTC1590.11074119990527798No Hit
AGCCACGGGAAATGCAATGAATGAATCATCCTTGTTATCCATCCAGTCCTGA1590.11074119990527798No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1580.1100447143712825No Hit
TGAAAAGGGAATCAGGCAAGAGAAATAAAAGGCATCAAAATTGAAAGGAAAG1580.1100447143712825No Hit
TGCTAGGGGCTGGGCCTCTCAGGGTTTCACACGTACCTCGTCGCCTCCAACC1530.10656228670130521No Hit
GGACACGGGAGTTGGACAATTGCCATTTTAGAACATTTCCTGGAGCATAAGG1530.10656228670130521No Hit
CTCCGGGTGTTTTACAATATTGTTATCACTAATAGGAAATAAGTTTCATTTT1530.10656228670130521No Hit
GCCTTAGCCTCCCGAATATCTGGGACTACGAATATCTGGGACTGCAGGCGCC1520.10586580116730974No Hit
ATCAACGGGGATGGCTCTTGGTATAAGTCTCACTTAAGTTTCTCTGACGATT1510.10516931563331429No Hit
TTGCTGGGGGGGTCCGTGGACCAGGAGGATTGCCTTTAATATTTTGTAATAT1490.10377634456532339No Hit
TTGGGAGGGGCCTGGATGATTCTGCACAGCTTATACCAGGCACGGATTGTGA1490.10377634456532339No Hit
CTTTCCGGGGACGCTCTTCCGATCTGGCCACGGGGTGGTTTTTATGACCGGA1490.10377634456532339No Hit
TTAAAAGGGGACATGTGTAAGAAGCACTTTCAGAATGTTGTCCTTTTTAAGA1480.10307985903132792No Hit
AGCAGTGGGAATTCTTCATGGTGTATTTGCAAACTAACTTAACATAGATTCT1470.10238337349733247No Hit
TAGCTTGGGAGTACCGGAGGAGAAAGATGGCGGCTACCTTACTAGCTGCTCG1460.101686887963337No Hit
CAATGGGGGTCTTAAAATAATCTACATTTTTCTAATATGCAATTAAGGGAAC1460.101686887963337No Hit
GTAAACGGGCGATCTTTAGTTGGGAAGTCAAGATGAATGAGTTTGATTTTTA1460.101686887963337No Hit
AGCGCAGGGCACCGTTTCAAGCCTGCCCTGTGGAGGAAATGCCAGTTTATTA1460.101686887963337No Hit
ACAACAGGGAGAAGTATGCATAGAAAAAAGGTAAGCATTTTCCAATAGTGGT1460.101686887963337No Hit
AGAACAGGGATGGTAGGGAAGAAACTAGAAAATAGAAGAGCAAATTAAACTC1460.101686887963337No Hit
TGTTTCGGGCAGCAAGTGTTATCTTGCAGGTACAGTCAAGCTTCGCCTTCCT1450.10099040242934154No Hit
TAGTCAGGGAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGTCC1450.10099040242934154No Hit
ACGATTGGGGGGTTTCATAAAACCTCCAACCTGCACCCCTCCCTTCTCCATC1450.10099040242934154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)