FastQCFastQC Report
Sat 31 Dec 2022
SRR5844532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95385
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC14071.4750746972794464No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT12981.3608009645122399No Hit
CCGCGAGGGTAGCAGGAATGTTTTAATTTGTGCTTCCTTAGTAAATTGAAAT2320.24322482570634796No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT1980.2075798081459349No Hit
TGTTGAGGGATTTCAAATGAGGTTTTGAACAAGTTCAGAAAGTGGAACTTGA1870.19604759658227183No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1850.19395083084342402No Hit
AAACCAGGGAGGATAGCCCAAATGGCTATAAAAGAGGTCAAAAATTCTTTGT1640.17193479058552183No Hit
GTAGGGGGTAAAAAAGTACAGGATGGCTTATCTGATATTGCAGAGAAATTCC1610.1687896419772501No Hit
TACCTGGGGATTGTTTTTCATTTACTATCTTGATCATAGAGTTTGGCTGGGG1570.16459611049955444No Hit
TCTCAAGGGATCAAAGTAGTGATGGATTCAGTACTCCTCAACCACTCTCCTA1520.15935419615243487No Hit
GGATCAGGGAATTTAGTTAGCCTAAACAGAAATAGGAGATTAATATTTTGAG1510.15830581328301097No Hit
CGCACGGGGATTGCAGGAAAATCCAATTATATTCTAGTTCCTTCAATGAAGA1460.15306389893589137No Hit
AAAGTGGGGCTGGCCAGACTCTGTGTATCATGATTGCAGGAGCCTGGGTTTA1410.14782198458877183No Hit
GGTCCGGGGAAATATTAAAAGTATAAAAATATAATTTAAAAGACCCGTAAAA1400.14677360171934792No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA1400.14677360171934792No Hit
TCTTGGGGAGGTGTCCTTTTTATAAGGTGCACTTTTGGAGTTCTTCTGATGC1400.14677360171934792No Hit
AATAAAGGGAGGCTCACGGGCCACAAAGGTGCTTGATTGCAGCAGGTTCAAA1320.1383865387639566No Hit
TCCATGGGGATTGAGCAATACTTCATTGTATGAATACACCACAATTTATTTC1310.1373381558945327No Hit
GTAGTAGGGATCTTAACCATGCTAGTATAAGCTGCCATCAACTTCTCTGCTG1300.13628977302510878No Hit
CTCAGTGGGATTTTCAGAGGTAAGAAACTCATTAGAGGTGAGAAACTCAAGA1290.13524139015568484No Hit
TGTTCAGGGTTATTTCTCAGTTAATCATTTTGAGTATATGGAAACTGCTGAT1280.13419300728626093No Hit
AAGAAGGGGACCTTAACATCTTACAATGTGGAATTGGTTTAAATCTGAGATG1260.13209624154741312No Hit
TGGCGCTGGGGGAGGAGATGAAGTGGAATTTAAATGTTGGGAATCAAATCCA1250.13104785867798918No Hit
GAGGCTGGGGAAGTCCTTCCATCACAGATTCCAAGTGTTCCTTTTAAAATAC1240.1299994758085653No Hit
TGCATGGGGAAGACAGTTTGACAAGTGTGGCTTGGAATCCAGATGGGAAGCG1230.12895109293914137No Hit
GCTAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1140.11951564711432615No Hit
ATGGTGGGGGCGGTGGGGGAGGCTGTCTTTGTACCACTGCAGCATCCCCCAC1140.11951564711432615No Hit
CTTTCCGGGGCCATGAGGAACAAGGCAAGGCTGTCAATTACAGGAAGTACTG1130.11846726424490225No Hit
TTGTGCGGGAGAAAAGTTTTAACTTATTTTACTCCATATAGAAACTGTAGAG1110.11637049850605442No Hit
TGAAATGGGAGGGACTGTCTCATCTTTCTAATATCATCTATTTATCTAATTT1100.1153221156366305No Hit
TATTGAGGGAATTGCAAATTTGGGAATACGAGTTGTACAGTTCAGTCATAAT1100.1153221156366305No Hit
TTCCGCGGGAGTACCACATTTTGAACCTATCCACACGAATCAGCCACAGCTT1100.1153221156366305No Hit
GACTAGGGACCATTTTTTTATTTAAAGAAACTTTGATCTGCAAACCTCAAAG1100.1153221156366305No Hit
ACCTAGGGGCTGGCTTAACAGAATTGTTCTTGTGTTAGCTCATCCCAGGGAA1070.11217696702835876No Hit
TATACTGGGTACCCGGTAGTGGGATTGCTGGATCAAATAGTAGATCTACTTT1040.10903181842008701No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1030.10798343555066311No Hit
AGCAGGGAAGGAAGAGATAATGTTTATGAGAGGTGAGTCTTTATGTGTTTTA990.10378990407296745No Hit
GCATTGGGGGGGAACACAAAAATGTCACTTTTCCTCTTTAAGCTGCCTAACG990.10378990407296745No Hit
GCCCACGGGTACGCGCGGGACTCCTCAAGAAGAGCAACCCCAAGACACATAT990.10378990407296745No Hit
TAATCAGGGGAGCTTGTCACATTCTAATCCCTCTCCCCATCCTGTTTCAATT980.10274152120354353No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC960.1006447554646957No Hit
CTTGTCGGGATGAAAAGGAAATGAATGGATTCGAATGAAATTGTCCTTTAGA960.1006447554646957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)