FastQCFastQC Report
Sat 31 Dec 2022
SRR5844531.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844531.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94314
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC13461.4271476132917702No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT13111.3900375341942872No Hit
CCGCGAGGGTAGCAGGAATGTTTTAATTTGTGCTTCCTTAGTAAATTGAAAT2350.24916767394024217No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT1850.19615327522955234No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1850.19615327522955234No Hit
TCCATGGGGATTGAGCAATACTTCATTGTATGAATACACCACAATTTATTTC1840.19509298725533855No Hit
TGTTGAGGGATTTCAAATGAGGTTTTGAACAAGTTCAGAAAGTGGAACTTGA1670.17706809169370402No Hit
TCTCAAGGGATCAAAGTAGTGATGGATTCAGTACTCCTCAACCACTCTCCTA1580.16752549992577984No Hit
GGTCCGGGGAAATATTAAAAGTATAAAAATATAATTTAAAAGACCCGTAAAA1580.16752549992577984No Hit
AAACCAGGGAGGATAGCCCAAATGGCTATAAAAGAGGTCAAAAATTCTTTGT1550.16434463600313845No Hit
TGGCGCTGGGGGAGGAGATGAAGTGGAATTTAAATGTTGGGAATCAAATCCA1540.16328434802892466No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA1520.16116377208049706No Hit
GTAGGGGGTAAAAAAGTACAGGATGGCTTATCTGATATTGCAGAGAAATTCC1420.1505608923383591No Hit
GGATCAGGGAATTTAGTTAGCCTAAACAGAAATAGGAGATTAATATTTTGAG1390.1473800284157177No Hit
TGTTCAGGGTTATTTCTCAGTTAATCATTTTGAGTATATGGAAACTGCTGAT1370.14525945246729013No Hit
CTCAGTGGGATTTTCAGAGGTAAGAAACTCATTAGAGGTGAGAAACTCAAGA1300.13783743664779355No Hit
CGCACGGGGATTGCAGGAAAATCCAATTATATTCTAGTTCCTTCAATGAAGA1270.13465657272515213No Hit
ACCTAGGGGCTGGCTTAACAGAATTGTTCTTGTGTTAGCTCATCCCAGGGAA1270.13465657272515213No Hit
AAAGTGGGGCTGGCCAGACTCTGTGTATCATGATTGCAGGAGCCTGGGTTTA1260.13359628475093838No Hit
TACCTGGGGATTGTTTTTCATTTACTATCTTGATCATAGAGTTTGGCTGGGG1250.13253599677672456No Hit
GCTAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1250.13253599677672456No Hit
GTAGTAGGGATCTTAACCATGCTAGTATAAGCTGCCATCAACTTCTCTGCTG1230.13041542082829696No Hit
AAGAAGGGGACCTTAACATCTTACAATGTGGAATTGGTTTAAATCTGAGATG1220.12935513285408318No Hit
CTTTCCGGGGCCATGAGGAACAAGGCAAGGCTGTCAATTACAGGAAGTACTG1190.1261742689314418No Hit
TGAAATGGGAGGGACTGTCTCATCTTTCTAATATCATCTATTTATCTAATTT1180.12511398095722798No Hit
GAGGCTGGGGAAGTCCTTCCATCACAGATTCCAAGTGTTCCTTTTAAAATAC1180.12511398095722798No Hit
CCAGCGGGGCAGATCTCGCTTTTCAAAATGTGTCTAGAGCTGGTGGCTGTAA1170.12405369298301419No Hit
AATAAAGGGAGGCTCACGGGCCACAAAGGTGCTTGATTGCAGCAGGTTCAAA1140.1208728290603728No Hit
TGCATGGGGAAGACAGTTTGACAAGTGTGGCTTGGAATCCAGATGGGAAGCG1120.1187522531119452No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1080.11451110121509002No Hit
GCCCACGGGTACGCGCGGGACTCCTCAAGAAGAGCAACCCCAAGACACATAT1080.11451110121509002No Hit
TTAGCCGGGAGCTCTGGATACAGAAAGTGAAAAGGTTGTCCAAGAAGCCCTG1080.11451110121509002No Hit
TCTTGGGGAGGTGTCCTTTTTATAAGGTGCACTTTTGGAGTTCTTCTGATGC1080.11451110121509002No Hit
TTTGCAGGGGTCCTTTAAATGGATAACAGTATGTGTTCTAATGGCATAAAAT1050.11133023729244862No Hit
TTTGATGGGGGGGTGGTGCTGGCTCTGATGATTCCAGAGCCTGTATCCACCT1040.11026994931823485No Hit
TTCCGCGGGAGTACCACATTTTGAACCTATCCACACGAATCAGCCACAGCTT1030.10920966134402103No Hit
TATTGAGGGAATTGCAAATTTGGGAATACGAGTTGTACAGTTCAGTCATAAT1010.10708908539559345No Hit
TATACTGGGTACCCGGTAGTGGGATTGCTGGATCAAATAGTAGATCTACTTT1010.10708908539559345No Hit
GACTAGGGACCATTTTTTTATTTAAAGAAACTTTGATCTGCAAACCTCAAAG1010.10708908539559345No Hit
TAATCAGGGGAGCTTGTCACATTCTAATCCCTCTCCCCATCCTGTTTCAATT990.10496850944716585No Hit
AGCAGGGAAGGAAGAGATAATGTTTATGAGAGGTGAGTCTTTATGTGTTTTA970.10284793349873826No Hit
CTTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.10178764552452445No Hit
ATGGTGGGGGCGGTGGGGGAGGCTGTCTTTGTACCACTGCAGCATCCCCCAC960.10178764552452445No Hit
TATAAAGGGAATCCAGCCAAGAGTGGTTCCTAATGCATCATTCTTCAACTTC960.10178764552452445No Hit
TAAAGCGGGTAAATTATCTTCTTAATGCCTCTTAAACCACTTACATTCAAAG950.10072735755031068No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)