FastQCFastQC Report
Sat 31 Dec 2022
SRR5844530.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844530.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94350
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC13101.388447270800212No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT12831.359830418653948No Hit
CCGCGAGGGTAGCAGGAATGTTTTAATTTGTGCTTCCTTAGTAAATTGAAAT2060.2183359830418654No Hit
TCCATGGGGATTGAGCAATACTTCATTGTATGAATACACCACAATTTATTTC1920.20349761526232113No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT1890.20031796502384736No Hit
CTCAGTGGGATTTTCAGAGGTAAGAAACTCATTAGAGGTGAGAAACTCAAGA1810.19183889772125068No Hit
AAACCAGGGAGGATAGCCCAAATGGCTATAAAAGAGGTCAAAAATTCTTTGT1780.1886592474827769No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC1760.18653948065712772No Hit
TGGCGCTGGGGGAGGAGATGAAGTGGAATTTAAATGTTGGGAATCAAATCCA1630.17276099629040806No Hit
TGTTGAGGGATTTCAAATGAGGTTTTGAACAAGTTCAGAAAGTGGAACTTGA1600.16958134605193428No Hit
AAGAAGGGGACCTTAACATCTTACAATGTGGAATTGGTTTAAATCTGAGATG1540.16322204557498676No Hit
GGATCAGGGAATTTAGTTAGCCTAAACAGAAATAGGAGATTAATATTTTGAG1540.16322204557498676No Hit
GGTCCGGGGAAATATTAAAAGTATAAAAATATAATTTAAAAGACCCGTAAAA1520.16110227874933758No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA1510.160042395336513No Hit
TGTTCAGGGTTATTTCTCAGTTAATCATTTTGAGTATATGGAAACTGCTGAT1470.15580286168521462No Hit
TACCTGGGGATTGTTTTTCATTTACTATCTTGATCATAGAGTTTGGCTGGGG1390.14732379438261792No Hit
GTAGGGGGTAAAAAAGTACAGGATGGCTTATCTGATATTGCAGAGAAATTCC1380.14626391096979333No Hit
TCTCAAGGGATCAAAGTAGTGATGGATTCAGTACTCCTCAACCACTCTCCTA1360.14414414414414414No Hit
AAAGTGGGGCTGGCCAGACTCTGTGTATCATGATTGCAGGAGCCTGGGTTTA1360.14414414414414414No Hit
GCTAAAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1300.1377848436671966No Hit
GTAGTAGGGATCTTAACCATGCTAGTATAAGCTGCCATCAACTTCTCTGCTG1290.13672496025437203No Hit
TGCATGGGGAAGACAGTTTGACAAGTGTGGCTTGGAATCCAGATGGGAAGCG1280.13566507684154744No Hit
TTCCGCGGGAGTACCACATTTTGAACCTATCCACACGAATCAGCCACAGCTT1170.12400635930047695No Hit
TTTGCAGGGGTCCTTTAAATGGATAACAGTATGTGTTCTAATGGCATAAAAT1160.12294647588765237No Hit
CGCACGGGGATTGCAGGAAAATCCAATTATATTCTAGTTCCTTCAATGAAGA1150.12188659247482778No Hit
ATGGTGGGGGCGGTGGGGGAGGCTGTCTTTGTACCACTGCAGCATCCCCCAC1140.12082670906200317No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1130.1197668256491786No Hit
CCAGCGGGGCAGATCTCGCTTTTCAAAATGTGTCTAGAGCTGGTGGCTGTAA1130.1197668256491786No Hit
TTGTGCGGGAGAAAAGTTTTAACTTATTTTACTCCATATAGAAACTGTAGAG1100.11658717541070482No Hit
TAGTGAGGGGCAAGGAAGAAGGTATTCTGTTTATTTCCTGTCCACTGACTGA1100.11658717541070482No Hit
CTTTCCGGGGCCATGAGGAACAAGGCAAGGCTGTCAATTACAGGAAGTACTG1100.11658717541070482No Hit
TAATCAGGGGAGCTTGTCACATTCTAATCCCTCTCCCCATCCTGTTTCAATT1070.11340752517223106No Hit
TATTGAGGGAATTGCAAATTTGGGAATACGAGTTGTACAGTTCAGTCATAAT1050.11128775834658187No Hit
CAGGCTGGTATCAAAACTTACCTATCAAGGAAAGTGATGACTGCAGAAACCA1050.11128775834658187No Hit
GAGGCTGGGGAAGTCCTTCCATCACAGATTCCAAGTGTTCCTTTTAAAATAC1050.11128775834658187No Hit
AATAAAGGGAGGCTCACGGGCCACAAAGGTGCTTGATTGCAGCAGGTTCAAA1050.11128775834658187No Hit
TGAAATGGGAGGGACTGTCTCATCTTTCTAATATCATCTATTTATCTAATTT1040.11022787493375728No Hit
TCATATGGGACTGTTAGAAAATCCTAACATTGGTCTCCGTGCATGTGTTCAC1040.11022787493375728No Hit
ACCTAGGGGCTGGCTTAACAGAATTGTTCTTGTGTTAGCTCATCCCAGGGAA1020.10810810810810811No Hit
ACAAGGGAGAAATGATCTGGTATGTGATGTTCCCCACCCTGTGTCCAAGTGT1000.10598834128245893No Hit
AGCAGGGAAGGAAGAGATAATGTTTATGAGAGGTGAGTCTTTATGTGTTTTA1000.10598834128245893No Hit
TCTTGGGGAGGTGTCCTTTTTATAAGGTGCACTTTTGGAGTTCTTCTGATGC1000.10598834128245893No Hit
CTTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.10492845786963434No Hit
GCCCACGGGTACGCGCGGGACTCCTCAAGAAGAGCAACCCCAAGACACATAT990.10492845786963434No Hit
GCATTGGGGGGGAACACAAAAATGTCACTTTTCCTCTTTAAGCTGCCTAACG970.10280869104398517No Hit
CTTGTCGGGATGAAAAGGAAATGAATGGATTCGAATGAAATTGTCCTTTAGA970.10280869104398517No Hit
GCACTCGGGACTTTGAGACGGAGGTTTGAGCTGAGTACTGCTGTGGAAAGCT970.10280869104398517No Hit
ACAAGAGGGGTACGTAAATGTTCACAGATGCACTCTTTACAGTAGTCAAAAA950.10068892421833599No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)