FastQCFastQC Report
Sat 31 Dec 2022
SRR5844529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88617
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC24712.788404030829299No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT21832.4634099552004693No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3960.4468668539896408No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT3540.3994718846271032No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3170.35771917352201044No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2370.2674430414028911No Hit
CTGCATGGGCAGGCCACGGTGCAGGCCTGAGTCCTTCCACCGGCCCCGTCCA1940.21891962038886442No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1920.21666271708588644No Hit
GTAATGGGACATCAAGAGGCTAAAGAAGAAAGTCCTGTGATCATATCAATAG1920.21666271708588644No Hit
GGTCTTGGGAGAGCCTAAGTAGGAGCATGGTGTTCTTGTAATAGCAATCCAA1850.20876355552546352No Hit
TACTACGGGAATGAGTTTCTGCTGACTTGCTAGTCTTAGCAGGAGGTTGTAT1840.20763510387397452No Hit
TTACAAGGGAGGAAGAAAACTGTGTAGGCAGTTCTTCAAGGGAGAAAGAAAG1790.20199284561652955No Hit
GTGGGCGGGGTTACCATGGAAACTGAGTGAATGGTTTGAAATGAAGACTTTG1760.19860749066206257No Hit
GTTACACGGGCAAATGAGAGAAGGTGGAGTTATTGTTGATTACCATGGTTGT1700.19183678075312863No Hit
CAAGCTGGGGGAAGACTTTTAAAAGACCGTTATTCTTTCTTAAACATTTGAA1670.18845142579866164No Hit
ACAAGTGGGTTCGCCATGTGATGACAAACCTTGGAGAGAAGTTAACAGATGA1640.18506607084419469No Hit
AGCTCTGGGACTGCAGGTGTTACACGTTCAGCTTCAAGTCCCAGACTTTCCT1630.18393761919270568No Hit
GCAGTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1600.1805522642382387No Hit
CTAGCGGGACCTGTGCACACCTGCTCAGAGCACGGCTCCTCGCAGGGGTGAA1600.1805522642382387No Hit
GACATCTGGGAGTTAGGGATGAGCTGATTCTCATATTTTTCCAAGATACATT1600.1805522642382387No Hit
TTTCCCGGGAAGCTTGAACCAGTTTCCATCTGGAAAGATTCTAAAATATAAT1580.17829536093526074No Hit
AATCACGGGAAACAAAAAAATCGTCAACGGCAGTTCCTTCAGATAATCAACA1580.17829536093526074No Hit
TGTTCAGGGGTAATATGGTAAATACTGGATTCCAGATTGCATTCCTATTTCC1570.17716690928377174No Hit
ATCACGGGGGGGCGCGGCGACGACCGCCGGGAGCGTGTGCAGCGGCGGCGGC1560.17603845763228274No Hit
CTAAAAGGGACTTGAAATAGAGGGAGCTAGAACACTTAATGTTTAATCTGTT1550.17491000598079373No Hit
GGTGGACGGGAGAGGAGGCCTCTACCACCCCTGAAGAAGCCTCGAGCACTGC1550.17491000598079373No Hit
CTATACGGGAGGGTAAATAAATAATTTAACAAATTTGATCAGCATCTCTAGT1530.17265310267781578No Hit
AGAGAGGGGGGCTCATTGCAACCTCCGCCTCCCGGGTTCACGCCATTCTCCT1520.17152465102632677No Hit
CACACTGGGGCCATTCAAATGCCTCCTAACTTTTATAGTTATTTTGTTTTAT1500.1692677477233488No Hit
ATAAGGGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1490.16813929607185982No Hit
GCTAGGGGGGTAACTAAGCATTTGTGTTCTGATATCTGAGGCCAGTAACTAT1490.16813929607185982No Hit
TCTCAGGGCATGTAAAGTAAAAACTATTCACCTTCTCACCTCTCTGGATGAA1470.1658823927688818No Hit
AAGGTAGGGCTTGCAAGCGTGGGCAAGTTCTTCACCCCTTCTGAGTCTGTTC1470.1658823927688818No Hit
TTGCGAAGGGGTGTTAATGTTGCCTAAAGTCACAAATACTTTTATTTTTCTT1440.16249703781441485No Hit
GTCCTGAGGGCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGG1430.16136858616292585No Hit
ATCTACGGGATTTCTAAATGAAAAATCATAGGTATTTGTTACCATGTGTGAA1430.16136858616292585No Hit
GATTTAGGGAGTTGGATTTCATTATCTTAGTGAATTTAGTCATTTTACAATA1420.16024013451143684No Hit
TAGCAGGGGATTGCCAATAAAAACAAAAATAGAATCCAGAGTGAAAATGATT1410.15911168285994787No Hit
CACTTTGGGAGAGACTGAGGCTGTAAGACGCATTGTGGAGAGGGATGGCCTC1410.15911168285994787No Hit
AATTATGGGTGGACGAATAGAAGTAAATATGTTTATTGGTGAAAAAGAATTC1390.1568547795569699No Hit
GCAGCAGGGAGAGATTTTCTATTTTATCATTATTTCAAAAGAACTCTTACCT1380.15572632790548088No Hit
CATCTTGGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGT1380.15572632790548088No Hit
AGGGCACCTTCAGCAACCTGTGTGGCTGTGATGAGCCCCTGAGCTCCGTGTG1370.1545978762539919No Hit
CTAATAGGGGCTTCCGGTGCGACGCTGTCTCTCCATGCCAGGACTGAGTTGT1370.1545978762539919No Hit
ATTACGGGGTTACTCGGTGCAGTCGGACATCTGGAGCATGGGCCTGTCCCTG1350.15234097295101393No Hit
GTTTTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1350.15234097295101393No Hit
CATATCGGGATGAGATGAAATGATGAAATGAAATGATGGATGAAATGATGAG1340.15121252129952492No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1340.15121252129952492No Hit
GACAAGGGGAGCCCAAACAGACATTGTATTTCCTCAATGTTGGTTTAAGAGT1330.15008406964803594No Hit
AAACAGGGGATTTATGCTGAATTGTGATTTTTTTATGCCAAATTTTTTTTAG1320.14895561799654694No Hit
AATTAGGGGAAGAGGAAACGGAAAAGAGATGGTGGAGAAACCTTTAGATTAT1310.14782716634505794No Hit
ATCTTTGGGAAGACTAATCCCATTGACTGTTTATTAAGCACCTACTGTGTGC1300.14669871469356896No Hit
CTTGCGAGGGGATGAATGAAAAATTGAAATTATCCAAAAGGATTTGGTGCTG1260.14218490808761297No Hit
GCGTGCGGGATTTATAAAAAGTACCTGTATCAAAGGAATTTTAACAAAGAGC1250.141056456436124No Hit
CAGGGCGGGATCCACCGGCGAAAGAGCTTTGACGCCTCTGACACACTGGCCC1250.141056456436124No Hit
ATTTATGGGAGAAGTTATCAGGAATGTGTTTAAAACAAGAAGAGAACTTTTC1240.13992800478463502No Hit
TAATACGGGGCAGAGCCGGCGCCGGAGGAGACGCACGCAGCTGACTTTGTCT1240.13992800478463502No Hit
GAAAGCGGGGTTTTACATTAAAAGAGAATGTCTTTGATCACTAGAGTCTGTC1230.13879955313314601No Hit
GTCTCGGGGAGACTCAAAATGTTTAGAGAAGTGCATTAACTTTTTCAGTTCA1210.136542649830168No Hit
TTCTCCGGGACCGTGGGGCAGATGAACTTGTGTCAACCATGCACACCCTGTG1200.13541419817867906No Hit
AGCACAGGGGTCCAGTAGGGATCCATGAATAAACTCTCAGTTATTTTGAAAT1200.13541419817867906No Hit
CACATAGGGTATTCCTTTTTAGTAGGAATCCGGAAGTATTAGATTGAATGGA1200.13541419817867906No Hit
TTGGGTGGGAGATCATGGTCAACTGGCCAGGCACAGTGGCTCACACAAGTAA1190.13428574652719005No Hit
ATCATCGGGATCACTGAGGGACCCAGCAGGACACATAAGGCATACTCTGGAG1180.13315729487570105No Hit
TGTAGCGGGTACACGACGCCTTCTGATCTTAGCAAGGGTGACTTTTGTCTTG1180.13315729487570105No Hit
TATATGGGACTATTAGGTTTTCTAGTAAGCTTTCCACTATTCCTGTAGAAAT1180.13315729487570105No Hit
CTTCAAGGGAAGCTTCTGAAGTGTTAGAGGTAGAGATGGTCTAGTAAAGATG1170.13202884322421204No Hit
CTGTGTGGGATTCCTCTTCCCCTTGGTAGCAGTTCCCTGACCAAGGGCAAGG1170.13202884322421204No Hit
GTTTGTGGGACAGTAACTTACAGAATTGACATTGTATAATTTGTCATTTTTT1160.13090039157272307No Hit
AGACAGGGGGTAACCTGCGTGGTGATGTGACAACTGTTTCCTCCCTGACCCC1160.13090039157272307No Hit
CTTTAAGGGGAAGACTAATCCCATTGACTGTTTATTAAGCACCTACTGTGTG1160.13090039157272307No Hit
ATAGTTGGGAGATGTACATTCCTGAGGTCTTTTATCTGTTGTAGCTTTGTCT1130.12751503661825608No Hit
ATAGCAGGGAGCGCAACTTTGAATGCTTTCTTACTTGTTTTGTGACCTAATT1110.1252581333152781No Hit
CGAAGAGGGAACAGCACCAGCAGGAGAGTGGGCTAGCCTGCTGGATGCTGTT1110.1252581333152781No Hit
GCTATAGGGCTGCCACATACTTGTGTACACCTTTGGACAAGGCATATAACTT1100.12412968166378913No Hit
CAAAATGGAGGTTAAGGCAGATTTATTTTTATGATTTGTTTAGGAATTATTT1070.12074432670932213No Hit
TTGCCAGGGTACTGCTGTATCTTTTCTACCTTCTTATTTGTAATAGAAACTA1070.12074432670932213No Hit
CGTAGAGGGAGAACAAAAAGAGGCCGAATTTCAGCAGATGCTCGAACAGCTT1060.11961587505783314No Hit
AATAACGGGAGACAAGAGACTAAAATAAAACAGAATAAAGTATGGAAAAGAA1050.11848742340634416No Hit
CTTTGGTTGGGATACTTTGTTGATTTGTCATATCTGGGTAAAAGGTTTATGG1040.11735897175485517No Hit
ACGCGCCTGGCTCTGCCACTGTTCTCTTCCCTCTGCTGCAAAGCCCAGTTAA1030.11623052010336617No Hit
TGGCAGGGAGAGGTTTTATATATATAAATCACATTTTTTCCCCAGCTTCTTA1020.11510206845187718No Hit
TTGTTCGGGAGGGGAGGCCGGGCCTTGCCCTCCCTTCTGCCCAGGGCCCAGT1020.11510206845187718No Hit
ACACGAAGGGATCAAGATAGTAGTATTATTACACAAGAAACTTGGTCTGCAG1020.11510206845187718No Hit
TAACCTGGGGGACAGTTAATGCCCACTGAAAGAAATTCATGAAAGAGAAGTA1020.11510206845187718No Hit
GAGTCAGGGCCACTGCGCCCGGCCTCAGCTTATTAATGTAGGTTTTCCTACA1010.11397361680038817No Hit
GATCCGGGGGAGTGATCCTGAGGAAGCTGATGTGTTATTCCTTCTCTGCATC1010.11397361680038817No Hit
AATCCAGGGCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCC1000.1128451651488992No Hit
ATCCATGGGAATGTTGTATAACTTTTATTCTTTATTATTGGTAACACGCCCT990.1117167134974102No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCC990.1117167134974102No Hit
ACAAATGGGGATACTTGGGATGAGTGAAAAATTACATCATTGGACATGAAGG990.1117167134974102No Hit
GTGTGAGGGACTATTCTGCTGTTGGCTGTAGGTACCTCTTCCATTGAATGAC980.11058826184592122No Hit
GTCGGGGTGTGTTTAAAGTCAACAATTCATTTTTAAGACTTTTTTCGAACTG980.11058826184592122No Hit
CGTAGCGGGGAGAGCTGGGCATTCAGGACTTCACAGTCCCCTTGCCCCTTTC980.11058826184592122No Hit
TTTACCGGGAGAGGGCACGTGGGAAATTTTCCAGCAATCAATGCCTTGAGAA970.10945981019443221No Hit
GCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTAC970.10945981019443221No Hit
AGCGAAGGGAATATGGTGTTCTTACAGAATATGAGCCCATTTGAAATAAGGT970.10945981019443221No Hit
GTAGGCGGGATTTCTGGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTA970.10945981019443221No Hit
GATAGGGGGATTCTAAGGAGTGACGTATTTTTCACCTGTTTGGATTCTATAT960.10833135854294322No Hit
TTTTGCGGGGGGGAGATTCCCTGTGAGTGCCAAGTATGTGAGATGCTTTCAG960.10833135854294322No Hit
TTAGCGGGAAATTACTGTTCATCAAATCTGAAATAGTCACCTCACAGCTCTT950.10720290689145424No Hit
GAATTAGGGACCCTCTCTACTTCTGGTTTTACTTTGTCTTCATGAATGCCCT950.10720290689145424No Hit
GTGTTGGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG950.10720290689145424No Hit
CGGGATGGGATTCTTCTGTAGAGGAAGAGAAAGGAGTGAAAGTTTAGCCCAC950.10720290689145424No Hit
CTTGTGGGATAAAAGGTTGAGTAAAAACAGGAAAGCGTGCTATAAGTTCAAA950.10720290689145424No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT950.10720290689145424No Hit
GTAATTGGGATAAACAATGGTCACGTTGTGATAGGGATAACAATAAATCTAT950.10720290689145424No Hit
TTGCCAGGATTTTGGGACTGCTGTGCCTCCTCCTCCTGTGGTGGAGGAGAAG950.10720290689145424No Hit
GTATTGGGGGGTTCCTAAGTTCTGTATTCAAGTTTTTGGAAATGTTTATTTA930.10494600358847625No Hit
TAAAGGGGGATCTTGAGCTGGCTAACAGATTTCCTCAGCAGGCCTGGGAAGA920.10381755193698726No Hit
TCTTTGGGAGTTTTCACCCCCTCAGAAACCACTGTCATTAGTTTACAAAGTT910.10268910028549826No Hit
TATGTTAGGGACTCCCTATCTGGTTGGAAGAGAGTAACCAGTTTCCAGAGAG910.10268910028549826No Hit
TGAAGGGGAAAGTCAAGAAAGATGGTTTTACCTTTACTGACCCAGCTGTACA900.10156064863400928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)