FastQCFastQC Report
Sat 31 Dec 2022
SRR5844528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87643
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC24652.8125463528176806No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT21722.4782355692981755No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA4300.4906267471446664No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT3530.4027703296327146No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2970.33887475326038585No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2360.26927421471195645No Hit
GTGGGCGGGGTTACCATGGAAACTGAGTGAATGGTTTGAAATGAAGACTTTG2060.23504444165535185No Hit
TACTACGGGAATGAGTTTCTGCTGACTTGCTAGTCTTAGCAGGAGGTTGTAT1980.2259165021735906No Hit
GCTAGGGGGGTAACTAAGCATTTGTGTTCTGATATCTGAGGCCAGTAACTAT1860.21222459295094873No Hit
ATCTACGGGATTTCTAAATGAAAAATCATAGGTATTTGTTACCATGTGTGAA1820.20766062321006812No Hit
TTTCCCGGGAAGCTTGAACCAGTTTCCATCTGGAAAGATTCTAAAATATAAT1770.20195566103396734No Hit
CAAGCTGGGGGAAGACTTTTAAAAGACCGTTATTCTTTCTTAAACATTTGAA1760.2008146685987472No Hit
GTAATGGGACATCAAGAGGCTAAAGAAGAAAGTCCTGTGATCATATCAATAG1720.19625069885786656No Hit
CTGCATGGGCAGGCCACGGTGCAGGCCTGAGTCCTTCCACCGGCCCCGTCCA1710.1951097064226464No Hit
CATCTTGGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGT1700.19396871398742627No Hit
CTAAAAGGGACTTGAAATAGAGGGAGCTAGAACACTTAATGTTTAATCTGTT1700.19396871398742627No Hit
GCAGTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1690.19282772155220612No Hit
GGTCTTGGGAGAGCCTAAGTAGGAGCATGGTGTTCTTGTAATAGCAATCCAA1670.1905457366817658No Hit
GACATCTGGGAGTTAGGGATGAGCTGATTCTCATATTTTTCCAAGATACATT1670.1905457366817658No Hit
AGCTCTGGGACTGCAGGTGTTACACGTTCAGCTTCAAGTCCCAGACTTTCCT1650.18826375181132549No Hit
TGTTCAGGGGTAATATGGTAAATACTGGATTCCAGATTGCATTCCTATTTCC1620.18484077450566502No Hit
GTTACACGGGCAAATGAGAGAAGGTGGAGTTATTGTTGATTACCATGGTTGT1610.18369978207044488No Hit
ATCACGGGGGGGCGCGGCGACGACCGCCGGGAGCGTGTGCAGCGGCGGCGGC1610.18369978207044488No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1580.18027680476478442No Hit
CTAGCGGGACCTGTGCACACCTGCTCAGAGCACGGCTCCTCGCAGGGGTGAA1570.17913581232956427No Hit
ATTTATGGGAGAAGTTATCAGGAATGTGTTTAAAACAAGAAGAGAACTTTTC1560.1779948198943441No Hit
AAGGTAGGGCTTGCAAGCGTGGGCAAGTTCTTCACCCCTTCTGAGTCTGTTC1530.17457184258868363No Hit
GCAGCAGGGAGAGATTTTCTATTTTATCATTATTTCAAAAGAACTCTTACCT1490.170007872847803No Hit
AATCACGGGAAACAAAAAAATCGTCAACGGCAGTTCCTTCAGATAATCAACA1480.16886688041258285No Hit
GTTTTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1470.1677258879773627No Hit
CTAATAGGGGCTTCCGGTGCGACGCTGTCTCTCCATGCCAGGACTGAGTTGT1460.16658489554214256No Hit
CATATCGGGATGAGATGAAATGATGAAATGAAATGATGGATGAAATGATGAG1450.1654439031069224No Hit
ATTACGGGGTTACTCGGTGCAGTCGGACATCTGGAGCATGGGCCTGTCCCTG1440.16430291067170225No Hit
CTATACGGGAGGGTAAATAAATAATTTAACAAATTTGATCAGCATCTCTAGT1440.16430291067170225No Hit
TTACAAGGGAGGAAGAAAACTGTGTAGGCAGTTCTTCAAGGGAGAAAGAAAG1410.16087993336604178No Hit
AATTATGGGTGGACGAATAGAAGTAAATATGTTTATTGGTGAAAAAGAATTC1400.15973894093082164No Hit
TTGCGAAGGGGTGTTAATGTTGCCTAAAGTCACAAATACTTTTATTTTTCTT1400.15973894093082164No Hit
GGTGGACGGGAGAGGAGGCCTCTACCACCCCTGAAGAAGCCTCGAGCACTGC1390.15859794849560147No Hit
AGAGAGGGGGGCTCATTGCAACCTCCGCCTCCCGGGTTCACGCCATTCTCCT1370.15631596362516118No Hit
GATTTAGGGAGTTGGATTTCATTATCTTAGTGAATTTAGTCATTTTACAATA1360.155174971189941No Hit
GACAAGGGGAGCCCAAACAGACATTGTATTTCCTCAATGTTGGTTTAAGAGT1360.155174971189941No Hit
AGCACAGGGGTCCAGTAGGGATCCATGAATAAACTCTCAGTTATTTTGAAAT1350.15403397875472086No Hit
AGGGCACCTTCAGCAACCTGTGTGGCTGTGATGAGCCCCTGAGCTCCGTGTG1350.15403397875472086No Hit
CAAAATGGAGGTTAAGGCAGATTTATTTTTATGATTTGTTTAGGAATTATTT1330.15175199388428054No Hit
ACAAGTGGGTTCGCCATGTGATGACAAACCTTGGAGAGAAGTTAACAGATGA1290.14718802414339993No Hit
ATCTTTGGGAAGACTAATCCCATTGACTGTTTATTAAGCACCTACTGTGTGC1290.14718802414339993No Hit
TCTCAGGGCATGTAAAGTAAAAACTATTCACCTTCTCACCTCTCTGGATGAA1290.14718802414339993No Hit
TGGCAGGGAGAGGTTTTATATATATAAATCACATTTTTTCCCCAGCTTCTTA1280.1460470317081798No Hit
GATCCGGGGGAGTGATCCTGAGGAAGCTGATGTGTTATTCCTTCTCTGCATC1280.1460470317081798No Hit
ATCATCGGGATCACTGAGGGACCCAGCAGGACACATAAGGCATACTCTGGAG1260.14376504683773947No Hit
CGGGATGGGATTCTTCTGTAGAGGAAGAGAAAGGAGTGAAAGTTTAGCCCAC1260.14376504683773947No Hit
GAAAGCGGGGTTTTACATTAAAAGAGAATGTCTTTGATCACTAGAGTCTGTC1260.14376504683773947No Hit
CTTTAAGGGGAAGACTAATCCCATTGACTGTTTATTAAGCACCTACTGTGTG1260.14376504683773947No Hit
TTGGGTGGGAGATCATGGTCAACTGGCCAGGCACAGTGGCTCACACAAGTAA1250.1426240544025193No Hit
TAATACGGGGCAGAGCCGGCGCCGGAGGAGACGCACGCAGCTGACTTTGTCT1250.1426240544025193No Hit
CACATAGGGTATTCCTTTTTAGTAGGAATCCGGAAGTATTAGATTGAATGGA1240.14148306196729915No Hit
AATTAGGGGAAGAGGAAACGGAAAAGAGATGGTGGAGAAACCTTTAGATTAT1220.13920107709685883No Hit
TAGCAGGGGATTGCCAATAAAAACAAAAATAGAATCCAGAGTGAAAATGATT1220.13920107709685883No Hit
GTTTGTGGGACAGTAACTTACAGAATTGACATTGTATAATTTGTCATTTTTT1220.13920107709685883No Hit
TATATGGGACTATTAGGTTTTCTAGTAAGCTTTCCACTATTCCTGTAGAAAT1220.13920107709685883No Hit
GCTATAGGGCTGCCACATACTTGTGTACACCTTTGGACAAGGCATATAACTT1210.1380600846616387No Hit
TTCTCCGGGACCGTGGGGCAGATGAACTTGTGTCAACCATGCACACCCTGTG1210.1380600846616387No Hit
TTGTTCGGGAGGGGAGGCCGGGCCTTGCCCTCCCTTCTGCCCAGGGCCCAGT1210.1380600846616387No Hit
CTTGCGAGGGGATGAATGAAAAATTGAAATTATCCAAAAGGATTTGGTGCTG1200.13691909222641854No Hit
CACACTGGGGCCATTCAAATGCCTCCTAACTTTTATAGTTATTTTGTTTTAT1200.13691909222641854No Hit
CACTTTGGGAGAGACTGAGGCTGTAAGACGCATTGTGGAGAGGGATGGCCTC1190.13577809979119837No Hit
ATAAGGGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1190.13577809979119837No Hit
AATAACGGGAGACAAGAGACTAAAATAAAACAGAATAAAGTATGGAAAAGAA1190.13577809979119837No Hit
GCGTGCGGGATTTATAAAAAGTACCTGTATCAAAGGAATTTTAACAAAGAGC1190.13577809979119837No Hit
TAAAGGGGGATCTTGAGCTGGCTAACAGATTTCCTCAGCAGGCCTGGGAAGA1180.13463710735597822No Hit
CGCTATGGGATGTGCAGGAAATGACAAATTTGTATGTCAGATTATACAAGGA1180.13463710735597822No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1180.13463710735597822No Hit
ATAGTTGGGAGATGTACATTCCTGAGGTCTTTTATCTGTTGTAGCTTTGTCT1170.13349611492075808No Hit
CGTAGCGGGGAGAGCTGGGCATTCAGGACTTCACAGTCCCCTTGCCCCTTTC1170.13349611492075808No Hit
CTTGTGGGATAAAAGGTTGAGTAAAAACAGGAAAGCGTGCTATAAGTTCAAA1160.1323551224855379No Hit
AAACAGGGGATTTATGCTGAATTGTGATTTTTTTATGCCAAATTTTTTTTAG1160.1323551224855379No Hit
GTCCTGAGGGCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGG1140.13007313761509762No Hit
TTAGCGGGAAATTACTGTTCATCAAATCTGAAATAGTCACCTCACAGCTCTT1120.1277911527446573No Hit
CGAAGAGGGAACAGCACCAGCAGGAGAGTGGGCTAGCCTGCTGGATGCTGTT1120.1277911527446573No Hit
AGCGAAGGGAATATGGTGTTCTTACAGAATATGAGCCCATTTGAAATAAGGT1120.1277911527446573No Hit
CTTTGGTTGGGATACTTTGTTGATTTGTCATATCTGGGTAAAAGGTTTATGG1110.12665016030943715No Hit
GCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTAC1110.12665016030943715No Hit
CAGGGCGGGATCCACCGGCGAAAGAGCTTTGACGCCTCTGACACACTGGCCC1110.12665016030943715No Hit
GTAGGCGGGATTTCTGGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTA1110.12665016030943715No Hit
AGACAGGGGGTAACCTGCGTGGTGATGTGACAACTGTTTCCTCCCTGACCCC1100.125509167874217No Hit
GTGTGAGGGACTATTCTGCTGTTGGCTGTAGGTACCTCTTCCATTGAATGAC1100.125509167874217No Hit
GTCGGGGTGTGTTTAAAGTCAACAATTCATTTTTAAGACTTTTTTCGAACTG1100.125509167874217No Hit
CTGTGTGGGATTCCTCTTCCCCTTGGTAGCAGTTCCCTGACCAAGGGCAAGG1100.125509167874217No Hit
AATCCAGGGCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCC1080.12322718300377669No Hit
ACAAATGGGGATACTTGGGATGAGTGAAAAATTACATCATTGGACATGAAGG1080.12322718300377669No Hit
ACGCCAGGGGTAGATCACTTTGGGTAGTATTGACATCTTAACAATATTAAGT1060.12094519813333637No Hit
ATCCATGGGAATGTTGTATAACTTTTATTCTTTATTATTGGTAACACGCCCT1030.11752222082767592No Hit
ATAGCAGGGAGCGCAACTTTGAATGCTTTCTTACTTGTTTTGTGACCTAATT1020.11638122839245577No Hit
GTATTGGGGGGTTCCTAAGTTCTGTATTCAAGTTTTTGGAAATGTTTATTTA1020.11638122839245577No Hit
CGTAGAGGGAGAACAAAAAGAGGCCGAATTTCAGCAGATGCTCGAACAGCTT1010.1152402359572356No Hit
TAGTCTGGGAGAATTACTATATGATCCAGCAAACCCACTGCTAGATATGTAG1010.1152402359572356No Hit
TAAATAGGGGCTCTCTGGTAAAGGCGTGCAGGTGTTGGCCGCGGCCTCTGAG1000.11409924352201546No Hit
TAGAACGGGCGAAACAAAGAGCTATAGAGGTTTTCCCCTTTCACAGTAGGGT990.1129582510867953No Hit
TGTAGCGGGTACACGACGCCTTCTGATCTTAGCAAGGGTGACTTTTGTCTTG960.10953527378113483No Hit
CTGCTAGGGATATTTTATTATTTAATCTTATGTGAAGATAGCATTGCATTTT960.10953527378113483No Hit
TAACCTGGGGGACAGTTAATGCCCACTGAAAGAAATTCATGAAAGAGAAGTA950.10839428134591467No Hit
ACTGTTGGGAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATT940.10725328891069451No Hit
GTCTCGGGGAGACTCAAAATGTTTAGAGAAGTGCATTAACTTTTTCAGTTCA940.10725328891069451No Hit
TGAAGGGGAAAGTCAAGAAAGATGGTTTTACCTTTACTGACCCAGCTGTACA930.10611229647547436No Hit
AGAGACGGGATGCCTCCCTGATAGACTTCAAATTCCATGGTGGTAGGAACCA930.10611229647547436No Hit
ACGCGCCTGGCTCTGCCACTGTTCTCTTCCCTCTGCTGCAAAGCCCAGTTAA930.10611229647547436No Hit
CTTCAAGGGAAGCTTCTGAAGTGTTAGAGGTAGAGATGGTCTAGTAAAGATG930.10611229647547436No Hit
TTTTTTGGGAGATTCAGTTTTTTGATATATCTTAATTGTGTACTTTGTGAAT930.10611229647547436No Hit
ATTCCGGGGGACCAGCACAAACTCCTGCTCTCCTGTGGTTATTTAATTCCGC920.1049713040402542No Hit
ACACGAAGGGATCAAGATAGTAGTATTATTACACAAGAAACTTGGTCTGCAG920.1049713040402542No Hit
TTGCCAGGGTACTGCTGTATCTTTTCTACCTTCTTATTTGTAATAGAAACTA910.10383031160503406No Hit
CTACCGAGGGGGAATAATATTCAGCAATAAAAAGGAACAAAGTATTCATATA910.10383031160503406No Hit
GTGTTGGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG900.1026893191698139No Hit
TTTACCGGGAGAGGGCACGTGGGAAATTTTCCAGCAATCAATGCCTTGAGAA890.10154832673459374No Hit
GTAATTGGGATAAACAATGGTCACGTTGTGATAGGGATAACAATAAATCTAT890.10154832673459374No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC890.10154832673459374No Hit
ATCATGGGGGTAAAGTAGGGATAATAAAAGTATCCATCTCACTGGGATATTT880.1004073342993736No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA880.1004073342993736No Hit
GTGCTTGGGATCTCTAGGGATGTTTAACATTTATAATTGCAAAATAAACCAA880.1004073342993736No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)