FastQCFastQC Report
Sat 31 Dec 2022
SRR5844527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87680
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC23082.6322992700729926No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT21762.4817518248175183No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3690.4208485401459854No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT3520.40145985401459855No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC2890.3296076642335766No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA2190.249771897810219No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC2060.23494525547445255No Hit
TACTACGGGAATGAGTTTCTGCTGACTTGCTAGTCTTAGCAGGAGGTTGTAT1910.2178375912408759No Hit
GCTAGGGGGGTAACTAAGCATTTGTGTTCTGATATCTGAGGCCAGTAACTAT1860.21213503649635035No Hit
ATCTACGGGATTTCTAAATGAAAAATCATAGGTATTTGTTACCATGTGTGAA1830.208713503649635No Hit
AGCTCTGGGACTGCAGGTGTTACACGTTCAGCTTCAAGTCCCAGACTTTCCT1810.20643248175182483No Hit
TTTCCCGGGAAGCTTGAACCAGTTTCCATCTGGAAAGATTCTAAAATATAAT1800.20529197080291972No Hit
CTAAAAGGGACTTGAAATAGAGGGAGCTAGAACACTTAATGTTTAATCTGTT1760.20072992700729927No Hit
GGTCTTGGGAGAGCCTAAGTAGGAGCATGGTGTTCTTGTAATAGCAATCCAA1740.19844890510948904No Hit
GCAGTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1730.19730839416058393No Hit
TTACAAGGGAGGAAGAAAACTGTGTAGGCAGTTCTTCAAGGGAGAAAGAAAG1710.19502737226277372No Hit
TGTTCAGGGGTAATATGGTAAATACTGGATTCCAGATTGCATTCCTATTTCC1700.19388686131386862No Hit
GTGGGCGGGGTTACCATGGAAACTGAGTGAATGGTTTGAAATGAAGACTTTG1670.1904653284671533No Hit
TCTCAGGGCATGTAAAGTAAAAACTATTCACCTTCTCACCTCTCTGGATGAA1670.1904653284671533No Hit
GACATCTGGGAGTTAGGGATGAGCTGATTCTCATATTTTTCCAAGATACATT1640.18704379562043796No Hit
CAAGCTGGGGGAAGACTTTTAAAAGACCGTTATTCTTTCTTAAACATTTGAA1610.18362226277372262No Hit
ATTTATGGGAGAAGTTATCAGGAATGTGTTTAAAACAAGAAGAGAACTTTTC1590.1813412408759124No Hit
GTTACACGGGCAAATGAGAGAAGGTGGAGTTATTGTTGATTACCATGGTTGT1590.1813412408759124No Hit
ATCACGGGGGGGCGCGGCGACGACCGCCGGGAGCGTGTGCAGCGGCGGCGGC1550.17677919708029197No Hit
AATTAGGGGAAGAGGAAACGGAAAAGAGATGGTGGAGAAACCTTTAGATTAT1540.17563868613138686No Hit
GCAGCAGGGAGAGATTTTCTATTTTATCATTATTTCAAAAGAACTCTTACCT1540.17563868613138686No Hit
CATCTTGGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGT1530.17449817518248176No Hit
AATCACGGGAAACAAAAAAATCGTCAACGGCAGTTCCTTCAGATAATCAACA1520.17335766423357665No Hit
AATTATGGGTGGACGAATAGAAGTAAATATGTTTATTGGTGAAAAAGAATTC1490.1699361313868613No Hit
CTGCATGGGCAGGCCACGGTGCAGGCCTGAGTCCTTCCACCGGCCCCGTCCA1450.16537408759124086No Hit
AAGGTAGGGCTTGCAAGCGTGGGCAAGTTCTTCACCCCTTCTGAGTCTGTTC1450.16537408759124086No Hit
GTCCTGAGGGCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGG1430.16309306569343066No Hit
TTGCGAAGGGGTGTTAATGTTGCCTAAAGTCACAAATACTTTTATTTTTCTT1410.16081204379562042No Hit
TATATGGGACTATTAGGTTTTCTAGTAAGCTTTCCACTATTCCTGTAGAAAT1380.1573905109489051No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1380.1573905109489051No Hit
TAGCAGGGGATTGCCAATAAAAACAAAAATAGAATCCAGAGTGAAAATGATT1370.15625No Hit
CATATCGGGATGAGATGAAATGATGAAATGAAATGATGGATGAAATGATGAG1370.15625No Hit
CTTTAAGGGGAAGACTAATCCCATTGACTGTTTATTAAGCACCTACTGTGTG1370.15625No Hit
AATAACGGGAGACAAGAGACTAAAATAAAACAGAATAAAGTATGGAAAAGAA1360.1551094890510949No Hit
ACAAATGGGGATACTTGGGATGAGTGAAAAATTACATCATTGGACATGAAGG1360.1551094890510949No Hit
CTGTGTGGGATTCCTCTTCCCCTTGGTAGCAGTTCCCTGACCAAGGGCAAGG1350.1539689781021898No Hit
TGGCAGGGAGAGGTTTTATATATATAAATCACATTTTTTCCCCAGCTTCTTA1340.15282846715328466No Hit
CTAATAGGGGCTTCCGGTGCGACGCTGTCTCTCCATGCCAGGACTGAGTTGT1320.15054744525547445No Hit
GCGTGCGGGATTTATAAAAAGTACCTGTATCAAAGGAATTTTAACAAAGAGC1310.14940693430656934No Hit
GATTTAGGGAGTTGGATTTCATTATCTTAGTGAATTTAGTCATTTTACAATA1300.1482664233576642No Hit
CACATAGGGTATTCCTTTTTAGTAGGAATCCGGAAGTATTAGATTGAATGGA1300.1482664233576642No Hit
CTAGCGGGACCTGTGCACACCTGCTCAGAGCACGGCTCCTCGCAGGGGTGAA1300.1482664233576642No Hit
GACAAGGGGAGCCCAAACAGACATTGTATTTCCTCAATGTTGGTTTAAGAGT1290.14712591240875914No Hit
CGAAGAGGGAACAGCACCAGCAGGAGAGTGGGCTAGCCTGCTGGATGCTGTT1290.14712591240875914No Hit
CGTAGCGGGGAGAGCTGGGCATTCAGGACTTCACAGTCCCCTTGCCCCTTTC1280.145985401459854No Hit
TTGGGTGGGAGATCATGGTCAACTGGCCAGGCACAGTGGCTCACACAAGTAA1270.1448448905109489No Hit
ATCTTTGGGAAGACTAATCCCATTGACTGTTTATTAAGCACCTACTGTGTGC1270.1448448905109489No Hit
GGTGGACGGGAGAGGAGGCCTCTACCACCCCTGAAGAAGCCTCGAGCACTGC1270.1448448905109489No Hit
GTAATGGGACATCAAGAGGCTAAAGAAGAAAGTCCTGTGATCATATCAATAG1270.1448448905109489No Hit
ATTACGGGGTTACTCGGTGCAGTCGGACATCTGGAGCATGGGCCTGTCCCTG1250.1425638686131387No Hit
CAAAATGGAGGTTAAGGCAGATTTATTTTTATGATTTGTTTAGGAATTATTT1250.1425638686131387No Hit
ATAAGGGGGGACAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGA1250.1425638686131387No Hit
GCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAGCTTCTGACTCTTAC1250.1425638686131387No Hit
GTTTGTGGGACAGTAACTTACAGAATTGACATTGTATAATTTGTCATTTTTT1240.14142335766423358No Hit
CTTGCGAGGGGATGAATGAAAAATTGAAATTATCCAAAAGGATTTGGTGCTG1240.14142335766423358No Hit
AGAGAGGGGGGCTCATTGCAACCTCCGCCTCCCGGGTTCACGCCATTCTCCT1230.14028284671532845No Hit
CTTTGGTTGGGATACTTTGTTGATTTGTCATATCTGGGTAAAAGGTTTATGG1220.13914233576642338No Hit
CACACTGGGGCCATTCAAATGCCTCCTAACTTTTATAGTTATTTTGTTTTAT1220.13914233576642338No Hit
CGTAGAGGGAGAACAAAAAGAGGCCGAATTTCAGCAGATGCTCGAACAGCTT1220.13914233576642338No Hit
TTCTCCGGGACCGTGGGGCAGATGAACTTGTGTCAACCATGCACACCCTGTG1200.13686131386861314No Hit
TATGCCGGGTAATACAGTTTCTACGTCTATTTAAGGCTAGGAGCCGAATGTG1200.13686131386861314No Hit
GAAAGCGGGGTTTTACATTAAAAGAGAATGTCTTTGATCACTAGAGTCTGTC1200.13686131386861314No Hit
CAGGGCGGGATCCACCGGCGAAAGAGCTTTGACGCCTCTGACACACTGGCCC1200.13686131386861314No Hit
GTTTTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1200.13686131386861314No Hit
TTAGCGGGAAATTACTGTTCATCAAATCTGAAATAGTCACCTCACAGCTCTT1190.13572080291970803No Hit
ACAAGTGGGTTCGCCATGTGATGACAAACCTTGGAGAGAAGTTAACAGATGA1180.13458029197080293No Hit
AGCACAGGGGTCCAGTAGGGATCCATGAATAAACTCTCAGTTATTTTGAAAT1180.13458029197080293No Hit
TGAAGGGGAAAGTCAAGAAAGATGGTTTTACCTTTACTGACCCAGCTGTACA1170.1334397810218978No Hit
CGGGATGGGATTCTTCTGTAGAGGAAGAGAAAGGAGTGAAAGTTTAGCCCAC1170.1334397810218978No Hit
ACGCCAGGGGTAGATCACTTTGGGTAGTATTGACATCTTAACAATATTAAGT1150.1311587591240876No Hit
AAACAGGGGATTTATGCTGAATTGTGATTTTTTTATGCCAAATTTTTTTTAG1150.1311587591240876No Hit
CACTTTGGGAGAGACTGAGGCTGTAAGACGCATTGTGGAGAGGGATGGCCTC1140.13001824817518248No Hit
AGGGCACCTTCAGCAACCTGTGTGGCTGTGATGAGCCCCTGAGCTCCGTGTG1120.12773722627737227No Hit
TAAAGGGGGATCTTGAGCTGGCTAACAGATTTCCTCAGCAGGCCTGGGAAGA1100.12545620437956204No Hit
TGTAGCGGGTACACGACGCCTTCTGATCTTAGCAAGGGTGACTTTTGTCTTG1100.12545620437956204No Hit
GATAGGGGGATTCTAAGGAGTGACGTATTTTTCACCTGTTTGGATTCTATAT1100.12545620437956204No Hit
ACACGAAGGGATCAAGATAGTAGTATTATTACACAAGAAACTTGGTCTGCAG1100.12545620437956204No Hit
CTATACGGGAGGGTAAATAAATAATTTAACAAATTTGATCAGCATCTCTAGT1090.12431569343065695No Hit
GTGTGAGGGACTATTCTGCTGTTGGCTGTAGGTACCTCTTCCATTGAATGAC1080.12317518248175183No Hit
GTAGGCGGGATTTCTGGGAGCACTTTGATAAGGACGGCTGGTCCCTGTGGTA1060.1208941605839416No Hit
GATCCGGGGGAGTGATCCTGAGGAAGCTGATGTGTTATTCCTTCTCTGCATC1060.1208941605839416No Hit
AGAGACGGGATGCCTCCCTGATAGACTTCAAATTCCATGGTGGTAGGAACCA1040.11861313868613138No Hit
CTAAAGGGGTTCGTAAATGAAGCAGAATTTTTTTTTCTCTTTTTTCCAAATA1030.11747262773722628No Hit
ACTGTTGGGAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATT1030.11747262773722628No Hit
CTTGTGGGATAAAAGGTTGAGTAAAAACAGGAAAGCGTGCTATAAGTTCAAA1020.11633211678832116No Hit
CTGAATGGGAATCATGTGAATGCAGAGCTGCAAATGGAAGTTATTGAATTGC1020.11633211678832116No Hit
ATCATCGGGATCACTGAGGGACCCAGCAGGACACATAAGGCATACTCTGGAG1010.11519160583941607No Hit
ATAGTTGGGAGATGTACATTCCTGAGGTCTTTTATCTGTTGTAGCTTTGTCT1010.11519160583941607No Hit
CTTCAAGGGAAGCTTCTGAAGTGTTAGAGGTAGAGATGGTCTAGTAAAGATG1010.11519160583941607No Hit
TGCCTAGGGAACTCAAGGAGGCTGGGTTTCTGGGCACCCTCGAAGTTTTCTG1010.11519160583941607No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT1000.11405109489051093No Hit
ATAGCAGGGAGCGCAACTTTGAATGCTTTCTTACTTGTTTTGTGACCTAATT1000.11405109489051093No Hit
TCTTTGGGAGTTTTCACCCCCTCAGAAACCACTGTCATTAGTTTACAAAGTT990.11291058394160584No Hit
CTACCAGGGACCAGGGAGAATTAAGCCTCTTGTTTTCCATTTATATTTATGC990.11291058394160584No Hit
TTGTTCGGGAGGGGAGGCCGGGCCTTGCCCTCCCTTCTGCCCAGGGCCCAGT990.11291058394160584No Hit
GTCTCGGGGAGACTCAAAATGTTTAGAGAAGTGCATTAACTTTTTCAGTTCA990.11291058394160584No Hit
TTGCCAGGGTACTGCTGTATCTTTTCTACCTTCTTATTTGTAATAGAAACTA980.11177007299270073No Hit
AGACAGGGGGTAACCTGCGTGGTGATGTGACAACTGTTTCCTCCCTGACCCC970.11062956204379562No Hit
ACGCGCCTGGCTCTGCCACTGTTCTCTTCCCTCTGCTGCAAAGCCCAGTTAA970.11062956204379562No Hit
GTCGGGGTGTGTTTAAAGTCAACAATTCATTTTTAAGACTTTTTTCGAACTG960.10948905109489052No Hit
TAGGTTGGGAGTATATTCAGCTTGAGAAATACCCCCAGGATCATGCAGCCAC960.10948905109489052No Hit
AGCGCTGGGAATGCTTTGCTGGTGCTCTGGCATTTTGTCTTTATCTCATCAC950.1083485401459854No Hit
TAGAACGGGCGAAACAAAGAGCTATAGAGGTTTTCCCCTTTCACAGTAGGGT950.1083485401459854No Hit
GATCACGGGGGGAAGGACCTCTTTGTTGCTTTCCCTTCTAAGTTGTCTTCTT940.1072080291970803No Hit
ATCCATGGGAATGTTGTATAACTTTTATTCTTTATTATTGGTAACACGCCCT940.1072080291970803No Hit
TTGGTTGGGAAGATATCTTATGTAGAAATATTTTGATAATATTTTTACAGTG940.1072080291970803No Hit
CGCTATGGGATGTGCAGGAAATGACAAATTTGTATGTCAGATTATACAAGGA940.1072080291970803No Hit
TTGCCAGGATTTTGGGACTGCTGTGCCTCCTCCTCCTGTGGTGGAGGAGAAG930.10606751824817517No Hit
GAATTAGGGACCCTCTCTACTTCTGGTTTTACTTTGTCTTCATGAATGCCCT920.10492700729927008No Hit
GTGACTGGGACTTCTATTCAGCATAGTACTGGAAGTCCTACCCAGAGCAATC920.10492700729927008No Hit
CATAACGGGATATTTTAAAAAAAAACTAGAATATTTCCCTGTTTGGTAAGTT910.10378649635036497No Hit
GATCCCGGGAGTCCAAAATTCAGGAACTACTAGAGTGAAATGATATTAAGTG910.10378649635036497No Hit
AATCCAGGGCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATGTCCTCC900.10264598540145986No Hit
AGCGAAGGGAATATGGTGTTCTTACAGAATATGAGCCCATTTGAAATAAGGT900.10264598540145986No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC900.10264598540145986No Hit
CTGCTAGGGATATTTTATTATTTAATCTTATGTGAAGATAGCATTGCATTTT900.10264598540145986No Hit
ACAACTGGGGGAGTCTACTGAACATCAAGCTTGCTATGAGCAGGATCTTAGA890.10150547445255474No Hit
CGTACTGGGAGAAGACCCCTGACTCATCATTTGTGGCAGTCCCTTATAATTG890.10150547445255474No Hit
ATTTACATGGCTTGAGAGTAATATAATCTGCCATACCATCCAGGAACCTACA890.10150547445255474No Hit
AACTCTGGGCGCTTTCAGAACCCCCTTGTTTTAGTATATGAAAAAGCCTAAT880.10036496350364964No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)