FastQCFastQC Report
Sat 31 Dec 2022
SRR5844523.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5844523.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63099
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGCCGTCT18412.917637363508138No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGTATGC16422.602259940728062No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCTTATAC3230.5118940078289671No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTA3150.49921551847097423No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCCTGTCTCT2200.3486584573448074No Hit
TGACTGGGGGTGAGGACTGTTCCCATCCAGGCCCAGCTGGGGCTTTCCCTTT1940.30745336693133013No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAA1930.305868555761581No Hit
TATTTGTGGGGTGACCAATAGCATCTCATGCATCCTAGAGCTTCCAGTCTAG1440.2282128084438739No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAACTCGTATGC1410.22345837493462653No Hit
AAACATGGGAGCTCTCAAAATCCACTGAAGAAGTCAAGTGAAGGTGGGTAAA1410.22345837493462653No Hit
TCGTCAGGGGCTCTCTCCTCCCGCCGCCCAAGATGCCGAAAGGAAAGAAGGC1410.22345837493462653No Hit
GCACTGGGGATATCCTGTGACTTAGAGTGTAGGATTTGTCATAAAAATCAGC1400.2218735637648774No Hit
TTGCATGGGCAACATATCCAAAATATTATATCAACAGGTAATTAATATAAAA1270.20127101855813878No Hit
GATCATGGGAGGAATTCAACATCTAGAAGTAATTCATAATATTTTTGGATAT1250.19810139621864054No Hit
TTGAAAGGGACAGCCAGCCCAGCTCCACTCAGGGCTGGCCCGGGGAGTCCCC1230.1949317738791423No Hit
TGTCGCGGGATAGGATATTAGTACATCGTACACGTGCTAGGAAAAAACAGCT1230.1949317738791423No Hit
CTGGGTGGGAGAATTCTTAAACTCTGTGATTTAGCTGTTTCAGTAAATAAAT1200.19017734036989492No Hit
ACAACCGGGCTGAAGACAGAGCAAAATGCTTTGAAAAGAATGAGGCCATACA1190.1885925292001458No Hit
GCTCCGGGGAGTTTCCATTTATGTCAATGCTAAATCCAAAGTCACTTCAGAG1180.18700771803039667No Hit
GTAGTAGGGCGATCTACGTGGGGGATCTGGAGGTGAGCAAAGTATGTTTGGT1180.18700771803039667No Hit
GCCACTGGGCCCTCGTTTACCTCAGAGAAGTAAATTTGATAGTGATGAGGAA1150.1822532845211493No Hit
ACGTTTGGGATTCCATTTTCCTAGTGCCACATGTGAAATTGGATTTTGATGA1140.18066847335140018No Hit
CTAACGGGGATGCCTTCACTACAACATTAGAATATTGTAACTCACATGCCTT1140.18066847335140018No Hit
CTCACTGGGATGAGAACTTCAGGAAAAAGCAGATTGAAGAGCTGAAGGGACA1100.1743292286724037No Hit
TCCAAAGGGAGACTAACAGTGGACTTCTCAGCAGAAACTTTACAAACCAGAA1090.17274441750265457No Hit
TTGCATGGGTGCAGAGGGGGGCCACAGTGGGCAGGTGGCACCGGGGGTCAGC1090.17274441750265457No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAAT1080.17115960633290545No Hit
GATGCTGGGAGTTCTCAGCTGTTTACCACAGCCCTCCAGGGTCCAAAGATTG1080.17115960633290545No Hit
AAGGCTGGGAATGTTGGGGAAAGTGAATTTTTCTCCTTTGTAGAAGGAGGTA1030.1632355504841598No Hit
CTTTTAGGGAGCAGCAGCATCGGAGGTACCCCCGCCGTCGCAGCCCCCGCGC1030.1632355504841598No Hit
ACCGCCGGGGAGAATCAAGCCAGCTGCAGAATTGGGGAGGAAGGGCTTTCCG1030.1632355504841598No Hit
CAAGCGGGGATGCCTTGAAAACCAGGAGAATATGGAATAAAACTTTTGAGAT1020.16165073931441068No Hit
ATGCCTGGGAGAGTTAAGCTACTAGACCTAGTGAAACAAAGAGGCTGAAATT1020.16165073931441068No Hit
AAACGCGGGATGTACAGATGTACAAACATCATTTTAGGGTTTAATTTGTTTT1020.16165073931441068No Hit
GCTGTAGGGATGTCTTTGTTATCTTATTACAAGAATTGTTATTTGAAAAAGA990.1568963058051633No Hit
ACACACGGGATTCAACACAGTTTTCAACAGTGCTACTGAAAGCTACAGGAGA980.1553114946354142No Hit
AGTCATGGGATGTACATAATGGAATACTATTCAGCCATAAAAAAGAATGAAA970.15372668346566506No Hit
GAAGTGGGGAGACATTAAAAGCATTGTTGGTGAGGACTTGAAAGGAAATGAG970.15372668346566506No Hit
TGTATGGGGATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG970.15372668346566506No Hit
TAGCTGGGGACATCACTGAAGATGGCACTGGGAAGCGATACACACAGCCAGC960.15214187229591594No Hit
GTTCTGGGGATTTGTATTAAACTTTTTCAATAAATATATACCAATTGATCCA960.15214187229591594No Hit
CATAGCGGGGCCACCATGCCTGGCTAATTTTTTGTGTGTTTTTAGTACAGAC960.15214187229591594No Hit
GTCATAGGGGGAGCTGGGACGGGCGGCGGCCGGGTGGCCTCGGCCACCCGCT950.15055706112616682No Hit
AACGGGAAGGCCAGCTACAGGTGGACTCCTGGAATTTGAGGCATCATAATGA940.1489722499564177No Hit
GCATGCGGGAAGGTCTCATCTCACTCTCTTGCCCAGGCTAGAGTGCAGTGGC940.1489722499564177No Hit
TAAATAGGGACTCCAGCCTGGGCAACAGGGCAAGAACCTGCCTCAAAAAAAG930.14738743878666857No Hit
TACATAGGGAGCTCTGGAAGGCCTTGGATGTCAGATTAAAGGACTGAGATTT930.14738743878666857No Hit
AACTTAGGGAGGAGAGGAAAAGGTGTAGGCCTGCCTTACCGAGAGCCAAACA930.14738743878666857No Hit
ATATATGGGAAGAAGTCTGATTAAAAGTTGAAATCAGTATTTCTGAATTCAA920.14580262761691942No Hit
ATGTCCGGGAGAAATGAAGTGGTTGTTATAAAAGGTTGAAATCACTAGGAAG910.1442178164471703No Hit
AAAGCTGGGGAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTTCTCGG910.1442178164471703No Hit
ATGCTAGGGATCCTTTTATAGGCATTACCTTTGCAGGGGGCACAATTTTCAC910.1442178164471703No Hit
TTAGATGGGATGTCAAGAGTTAAACCTCCTCAGGTTCAACCTGTGATAAAAG910.1442178164471703No Hit
ATGGCGGGATCTAAAAGCTGGGATAGTTCACCCCAAAAACTTTAAGTAGATC900.1426330052774212No Hit
CCCAAAGGGATAGATAACCAAATGGAAACATATCCCATGCTCATGGATGGGA900.1426330052774212No Hit
TGTCGCGGGAGTTGTTTTGGAAATTTTATGTAAAATTAAAACTATCAGTATT900.1426330052774212No Hit
TGGCTGGGATTGTTCTTCAAGCATGTTTTAGACCTAGAAAAGTATGGTTTGG900.1426330052774212No Hit
TGGCCGGGGCAAGATGTGCTTCTCTGCAATAACCAAAGACCTGAGCATGTCA890.14104819410767208No Hit
TTAGCAGGGAACCTCACTGAGTTAGAATCAGAGGTAAGTGGTATGCAGCTTA890.14104819410767208No Hit
TATCACGGGGTGTGGTAAAATTGGGGTAAAATGTGGTAAAATGTGATACGCA890.14104819410767208No Hit
CTGTTTAGGGTAGCTCCCACAGCTCCTTCAAGGGGCCGTGGGGGTGCCATTC890.14104819410767208No Hit
GGTAGCTTGGAGTCGAAGGGTGGTAGTACCCGAGCTATCAGCTGGTGTAGCC890.14104819410767208No Hit
ATCAACGGGCACTGAGATTATTCCTATAATTTTAAAGTATCATTTATTTGAA880.13946338293792296No Hit
TTATATGGGAGCTCATTTGTCCAAATTTCTGGGCTCAGCGCTTGGGAGGGCA880.13946338293792296No Hit
AATCCGGGATGAGAGAACTTGCCATGCTACGAAGACAGCAGATCAAGGTATT870.13787857176817384No Hit
ACATGTGGGGATCAAATAGAATGGCTTTCTGGAATGACTAATTTGAAATATT870.13787857176817384No Hit
CCTGACCTTGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGG870.13787857176817384No Hit
GCTGCGGGGAGACTTTGGAAGTGGCCTTGCCAGCGCTGTCCTGTCTTTCTGG870.13787857176817384No Hit
CTGTTTGGGATCATGGTGTAGTGTTCACTTACAAACATATCCTGTTGTTTCT860.13629376059842468No Hit
TAAGGTGGGAAGACATGGTTATTGACCTACTGCTTGACCAACTGACAGACCA860.13629376059842468No Hit
TGCCGTGGGAGTCCTAGGCTCTGTTTGCTTATACTAAATGTCTCTTTATCCT860.13629376059842468No Hit
CACTATGGGTATACATCTTAATTATGGTGGAATTCCTATGTAGAGTCTAAAA850.13470894942867556No Hit
AAGGTGGGATGACAGTAGCATACTGATGAGACAACACACACACACACAAAAC850.13470894942867556No Hit
AATCAAGGGTTAGCAAGAAAATGAAAGATATTCCTGAAAAATATTGCAAAAC850.13470894942867556No Hit
GACTAGGGGATTGCCCCTCCTCAGCTGTGCAAGGAGAAAGCATGCTTAGGAA830.13153932708917732No Hit
GCGCGGGGGCATCCGGCGACAGAGGACAGCCAGAAGCCCAGCCAGCCCCAGA830.13153932708917732No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCGT830.13153932708917732No Hit
TGCTAAGGGAAATATTCACATGTAATAAAATAATGTTAGCATATAAATATAC830.13153932708917732No Hit
TCTAGGGGGTGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG830.13153932708917732No Hit
AACTGGGGGGGTTTGCTTACGGTTCTGGTTTCCTACTCAGTGTCCGTGACCC820.1299545159194282No Hit
ATCTCCGGGAGTTTCATGTCCCTTAATAAATAATTAAGAAATCACTAAAATA820.1299545159194282No Hit
GATACTGGGTTATGCATTTATGCATGAGAACTAAATAGATTTTTAGATTCCT820.1299545159194282No Hit
TGTATCGGGATCTTACAGAAGTTGAGGCCAAGGGAGAATGGTAGGCACAGAA810.12836970474967907No Hit
ATTCTTGGGAGCAGCTCTCAAGCGTCACACTGCTCAGCTCCGCGAAGAGTTG810.12836970474967907No Hit
GTGCTAGGGACAGTGAAAATAGTTCTGAACGAGGACTTAAGGAGGGAAGTTG810.12836970474967907No Hit
TCACATGGGAACTAAGAACATTTTAAATGTACTTTCAAGTTTGTGTGACTAA810.12836970474967907No Hit
AATCTCGGGCGATCTAAAGACGGGCGTCTACCTGGTGTACGACCAGGAGCTG810.12836970474967907No Hit
GTATGGGGATTACAAAAGAAGAAACTGAGGGTCTCTGAGGTTAAGTAGCTTT800.12678489357992997No Hit
CGAAGCGGGACATATGTTTATTGCAGCACTATTCACAATAGCAAAGACTTGG800.12678489357992997No Hit
CTACGATGGGATTTCCCAGATTAAAAATACCAAAACTATTTTTTAAACTTAA800.12678489357992997No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTCTTATA790.12520008241018082No Hit
CAGAGCGGGATGACCTCACAGAAGGTGGCTCATGTATTGAATGGGAATTTGG790.12520008241018082No Hit
TCCGCAGGGACCAGAAAATATTTTTGTACCAGTAAAATTTAGCTGCAGTTGA790.12520008241018082No Hit
CTACGAGGGATAATGGTTTCAGTTAGTGAAGATGTTTATTTTAATAACTTTA790.12520008241018082No Hit
GCTCGGGGACGTGCGCCACCACACCCAGTTCATTTTTGTATTTTTAGTAGAG790.12520008241018082No Hit
TTGCCGGGGAAGTGCTTTATAGTTTACAGAGAGTTAAATATATATGTAGCAT790.12520008241018082No Hit
AATCAGGGGGACGCTCTTCCGATCTAACCTAGGGAACCCCCTAGGAATCACC770.12203046007068258No Hit
CGCTATGGGATGTGCATCTCTGTCAGCCTGAGTGGCTTTGTGGTCTTGGGCT770.12203046007068258No Hit
AAATGTGGGAGTTGACAGCATATTAATTGTGTGGCATTATAATTGTGAGAAA770.12203046007068258No Hit
CTTAAGGGGTATTATCAGCCCCATTTTATAGATAGACCCTAAGGCTTTGAGG760.12044564890093346No Hit
ATAATGGGGCTGGGCTACGCATTTCTCCTTACCATGGCCACAACCAGTGACA760.12044564890093346No Hit
GTGGACGGGAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACCT760.12044564890093346No Hit
AAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTC750.11886083773118433No Hit
CCGAGGGGGAAGTGGGGAGAAAACTTAGGGTGCTTCTGTGAATTAAAGCTAA750.11886083773118433No Hit
GATCCGGGGGTTTTGTAGAAGTACTTTATTACATATATGAAATAAACTATAA750.11886083773118433No Hit
GGCATAGGGAGAAGCGGATTCGGTAGGGTTTTAAACGCCTCTGCAGAAAACT740.11727602656143521No Hit
GATCGAGGGGGGGTCAGATAAAAAATGAATAAAGCAAGGATCTCTCTCTCTC720.11410640422193695No Hit
CTTGCAGCGGGAGCACCAGCGCCGGCTGCGTTCCGGGCCTCCGGTCGCCCGT710.11252159305218783No Hit
ATGACAGGGAGGCGAGGGCGCCGCACCCACACCGCGCTGCGCAGTTTTGTTC710.11252159305218783No Hit
GGTGATGGGAAATCCAGGCTATTAAAGTCACTAAATTTCTAAGTATGTCCAT710.11252159305218783No Hit
GTATATGGGAGGATTATTTAAACTATCCCAAACCTGGCTCAAATCTCTTAGA710.11252159305218783No Hit
CTCAATGGGTACGGATGACAGTTTGTTTAAACATACACCTACTAAAGGACAT710.11252159305218783No Hit
CGAGCAGGGAAGGAAAAAGGCATGCTTTCTGACTGACATGATCTTTTGCAAT710.11252159305218783No Hit
AGCTATGGGTTTCTTTTGATCATTTTTGTTCTTTATTCATTTGAACACGTTT700.1109367818824387No Hit
TTTACAGGGAAGGAGAAACAGCTTCAAAGGAGAGAGTTATTGGTGTGTGATT700.1109367818824387No Hit
TGCTATGGGATTTCCTAAATGGGATGCAAAAAGAAACTGAATGATGTTGAAT700.1109367818824387No Hit
TCACATAGGATTTCATCTTAGAAAAAGTTGCTTATATTCAGCAGGTTGGTTT700.1109367818824387No Hit
TCGATAGGGCCGATCTGTTGCTGGGAGCAGACTTTTAGTGGAAACCTTACAG700.1109367818824387No Hit
CTCCTAGGGATAACCAGCAACCATCAGAGCTACCAGCCCAAGCCATTCCATC690.1093519707126896No Hit
AAGAGTGGGATAATTTGAATCAACACAACCACCCACAGCCTAATTATTAGCA690.1093519707126896No Hit
CGTGAGGGAATAAATAGCTTCGGTAAGGAGTTAATTTCCTTCTAGAAATCAG680.10776715954294047No Hit
CAGCTGGGGCCTGTTAAAAATGTAAAAGCCATTCTTAGCTTATAGCCCATAC680.10776715954294047No Hit
CGCTTAGGGGATAAAAAGAAAAGAAATTGGATGTATTTACAGCTGTCCTTGA680.10776715954294047No Hit
TGACATGGGACCATTCCCCTATACTTTTTGTATTTTTTGTATAAACCATGCC670.10618234837319133No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGAGGCGTAATCTCTGTCTC670.10618234837319133No Hit
CTTAAGGGGATTGGCAATGGCGTTCATTCCTTACGAGTGGCTTTACTGCAGT670.10618234837319133No Hit
TCCGGCGGATTGTTAACAAGCATGTTCATCTTTTCTTGTCACTAGTCCAAGA660.10459753720344221No Hit
TCGTAAGGGAATGATATTTATTATTGAATATTGATTTGCTTATATAAATGTG660.10459753720344221No Hit
TACTAAGGGATCCGGCGCCGGCTTTCGGCGCGACGGTCGCCGCGTTCCATCG660.10459753720344221No Hit
GTCATTGGGAAGTCTATGTCATGAAGGGAAAATAAAAATGTATTCAGTAGCA650.10301272603369309No Hit
TAAAAGGGGGAGCATGGGCCTTGGGAGCCATTTGGAGGCTGGCTACCTCATG650.10301272603369309No Hit
CGACAAGGGATATATGCTCACTTCAATAAAAAGTTACTTATAAAAACAAACA650.10301272603369309No Hit
GAATTAGGGAGATTAACTTTTGGTCAAGGAGCTAAAGAACATCATGGCTTTA650.10301272603369309No Hit
ATACGCGGGATGGCAACACCCCGTCTCTAATAAAAATATAAAAATTAGCCTG640.10142791486394397No Hit
AATCAAGGGATGACAAGGAAACATCAGAATTACTAATCTAGCTAGTGTCATT640.10142791486394397No Hit
TGCTAGGGGGGCTGCTGGCCTCACATTTCCACTGGCAGTGGAGCCTTTCCCT640.10142791486394397No Hit
ACCATAGGGATCTGCCTATATGGGGGGTGGCGTTGGCCCCAAAGAGGCTTCA640.10142791486394397No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)